The role of cysteine peptidases in coronavirus cell entry and replication: The therapeutic potential of cathepsin inhibitors코로나바이러스 세포 진입 및 복제에서 시스테인 펩티다제의 역할: 카텝신 억제제의 치료 가능성Review Published on 2020-11-022022-08-13 Journal: PLoS Pathogens [Category] Biochemistry, COVID-19, [키워드] Activation antiviral drug cathepsin inhibitor Cathepsins cell entry cells chymotrypsin Coronaviral coronavirus CoV CoVs cysteine Diseases generate Host host cell humans inhibitor involved lysosomal nonstructural protein nonstructural proteins Nsps outcomes over Papain peptidase positive-sense prevalent processed replicase polyprotein replicase polyproteins Replication respiratory respiratory diseases RNA viruses severe respiratory diseases single-stranded spike glycoprotein spike glycoproteins Spike protein targets therapeutic potential treatment strategy utilization Viral Viral RNA viral RNA synthesis virus attenuation [DOI] 10.1371/journal.ppat.1009013 PMC 바로가기 [Article Type] Review
Structural insight into the recognition of S -adenosyl-L-homocysteine and sinefungin in SARS-CoV-2 Nsp16/Nsp10 RNA cap 2′-O-MethyltransferaseResearch Article Published on 2020-10-012022-10-31 Journal: Computational and Structural Biotechnology Journal [Category] COVID-19, [키워드] Abstract acute respiratory syndrome antiviral drug approaches atomic binding binding affinity binding mechanism caused Cell clinically complex coronavirus coronavirus disease COVID-19 effective enzyme Free energy calculation group H-bond inhibitor Innate immunity MD simulations mechanisms methyltransferase mimicking molecular Molecular dynamics simulation non-structural protein Nsp16/nsp10 Nsps nucleoside analog pandemic Rational drug design residue residues RNA SARS-CoV-2 Spread the SARS-CoV-2 transfer Vaccine virus detection [DOI] 10.1016/j.csbj.2020.09.032 PMC 바로가기 [Article Type] Research Article
The Enzymatic Activity of the nsp14 Exoribonuclease Is Critical for Replication of MERS-CoV and SARS-CoV-2Genome Replication and Regulation of Viral Gene Expression Published on 2020-09-162022-10-30 Journal: Journal of Virology [Category] COVID-19, SARS, [키워드] activities activity acute respiratory syndrome affecting Alpha Amino acid antiviral drug Betacoronavirus betacoronaviruses boost can be used complex coronavirus CoV CoVs Critical decrease displaying domains enzymatic activity enzyme evaluated example Exoribonuclease Frequency Genetic genetics genome replication guanine-N7-methyltransferase In vitro assay MERS-CoV MHV Middle East MONITOR mouse hepatitis virus mRNA mutant mutants Mutation nidovirus nonstructural protein nsp14 Nsps observation occurred phenotype Phenotypes progeny promote Proofreading purified replicase replicate Replication replication fidelity replicative reported respiratory syndrome coronavirus reverse genetics RNA genome RNA synthesis SARS-CoV SARS-CoV-2 sequence identity single mutant subunit Support thought viral RNA synthesis viruses while [DOI] 10.1128/JVI.01246-20 PMC 바로가기 [Article Type] Genome Replication and Regulation of Viral Gene Expression
Corona virus versus existence of human on the earth: A computational and biophysical approachReview Published on 2020-06-052023-06-15 Journal: International journal of biological macromolecules [Category] MERS, SARS, [키워드] antiviral drugs COVID-19 homology Nsps SARS-CoV-2 [DOI] 10.1016/j.ijbiomac.2020.06.007 PMC 바로가기 [Article Type] Review
Infectious Bronchitis Virus Nonstructural Protein 4 Alone Induces Membrane PairingArticle Published on 2018-09-062022-10-28 Journal: Viruses [Category] Coronavirus, [키워드] bronchitis Cell cellular membrane Combination coronavirus Coronaviruses Effect electron tomography Endoplasmic reticulum expressing expression Factor highlight Host IBV indicate induce Infectious Bronchitis Virus investigated mechanism membrane membrane rearrangements membrane vesicle Microscopy non-structural protein Nsp3 nsp4 Nsps organelle paired membranes Pairing pathogenic Proteins Replication replicative required responsible RNA viruses shown spherules strain structures transmembrane transmembrane protein vesicles Viral protein viral RNA synthesis virus were expressed zippered ER [DOI] 10.3390/v10090477 PMC 바로가기 [Article Type] Article
The ADRP domain from a virulent strain of infectious bronchitis virus is not sufficient to confer a pathogenic phenotype to the attenuated Beaudette strainShort Communication Published on 2018-08-122022-10-30 Journal: The Journal of General Virology [Category] Coronavirus, [키워드] ADP-ribose adRP avirulent Complete conserved coronavirus crystal structure demonstrated domain ENCODE IBV in vitro in vivo Infectious Bronchitis Virus investigated M41 macrodomain Modification motif Mutation non-structural protein NSP Nsp3 Nsps pathogenic pathogenicity phenotype predicted Protein restored strain virulence virulent [DOI] 10.1099/jgv.0.001098 PMC 바로가기 [Article Type] Short Communication
Crystal structure of Middle East respiratory syndrome coronavirus helicaseResearch Article Published on 2017-06-262022-10-29 Journal: PLoS Pathogens [Category] Coronavirus, [키워드] acute respiratory syndrome Analysis arterivirus C-terminal caused component conserved coronavirus CoV crystal structure domain domains drug design effective eukaryotic Fatality rate full-length Helicase information Interaction membrane MERS-CoV Middle East N-terminal nidoviruses nonstructural protein nsp13 Nsps Pandemics Protein Proteins public health remained replication-transcription complex respiratory syndrome coronavirus SARS-CoV SF1 targets Vaccine viral RNA synthesis while [DOI] 10.1371/journal.ppat.1006474 PMC 바로가기 [Article Type] Research Article
In Situ Tagged nsp15 Reveals Interactions with Coronavirus Replication/Transcription Complex-Associated ProteinsResearch Article Published on 2017-01-312022-10-29 Journal: mBio [Category] Coronavirus, [키워드] association biochemical carried complexes conserved coronavirus Coronavirus infection Coronaviruses CoV CoV infection CoV protein CoV replication defined determine domain Endoplasmic reticulum enzymatic activity enzyme epitope function genomic RNA help independent Infection interact Interaction interactions involved knowledge membrane MHV mouse hepatitis virus nonstructural protein not affect Nsp12 Nsp15 Nsps P/S polymerase Protein replaced Replication replication complex replicative required RNA RTC Tagged Transcription Viral protein viral replication virus virus replication while [DOI] 10.1128/mBio.02320-16 PMC 바로가기 [Article Type] Research Article
Infidelity of SARS-CoV Nsp14-Exonuclease Mutant Virus Replication Is Revealed by Complete Genome SequencingResearch Article Published on 2010-05-062022-10-29 Journal: PLoS Pathogens [Category] Coronavirus, [키워드] accumulate acute respiratory syndrome Author bioinformatics clone compared competent Complete complex consensus genome coronavirus coronavirus exoribonuclease Coronaviruses Critical Culture decrease deep sequencing demonstrated Effect Evolution examined experiment Frequency full-length genome Genetic Genome genome replication genome sequence growth Impact in vitro inactivation increase in Infidelity lack less limit mechanism MOST murine hepatitis virus mutant mutant virus Mutation nonstructural protein nonstructural protein 14 nsp14 nsp14-ExoN Nsps passage Pathogenesis powerful tool quasispecy recognize Replication replication fidelity required resulting Revealed RNA genome RNA genomes RNA virus SARS-coronavirus SARS-CoV sequence stability the Coronaviridae to define total population unique viral growth viral replication viral RNA genome viral system virus wild-type SARS-CoV [DOI] 10.1371/journal.ppat.1000896 PMC 바로가기 [Article Type] Research Article
SARS-Coronavirus Replication/Transcription Complexes Are Membrane-Protected and Need a Host Factor for Activity In VitroResearch Article Published on 2008-05-022022-10-29 Journal: PLoS Pathogens [Category] Coronavirus, [키워드] activity acute respiratory syndrome addition Author cause centrifugation characteristic complex complexes connection Critical cytoplasm cytoplasmic Degradation dependent on develop digestion dissect eight enzyme Enzymes Factor fraction functional genomic RNA Host host cell membranes host cells in vitro in vitro activity Infected infected cell life-threatening membrane membrane structure membrane structures membranes non-structural protein NSP Nsps polyproteins precursor produced protease PROTECT protein synthesis released remained replicate Replication required RNA RNA synthesis robust RTC SARS-coronavirus SARS-CoV SARS-CoV-infected cells Structure subgenomic mRNA subgenomic mRNA synthesis subunit susceptible Transcription Treatment viral genome replication Viral RNA viral RNA synthesis virus-induced membrane structure virus-induced membrane structures [DOI] 10.1371/journal.ppat.1000054 PMC 바로가기 [Article Type] Research Article