Using human iPSC-derived kidney organoids to decipher SARS-CoV-2 pathology on single cell level단일 세포 수준에서 SARS-CoV-2 병리를 해독하기 위해 인간 iPSC 유래 신장 오르가노이드 사용Article Published on 2022-07-192022-09-12 Journal: STAR protocols [Category] COVID19(2023년), MERS, SARS, 진단, [키워드] Cell Cell Biology cells changes in Complete kidney kidney organoid kidney organoids Microbiology organ Organoid organoids pathology protocol reveal SARS-CoV-2 Sequencing analysis Single Cell single cell level single cell suspensions Single-cell RNA sequencing STEM stem cells Transwell virus virus infection [DOI] 10.1016/j.xpro.2022.101612 PMC 바로가기 [Article Type] Article
Alternatives to animal models and their application in the discovery of species susceptibility to SARS-CoV-2 and other respiratory infectious pathogens: A reviewReview Published on 2022-07-012023-07-08 Journal: Veterinary pathology [Category] COVID19(2023년), [키워드] air–liquid interface COVID-19 in vitro organoids pathophysiology primary cell culture respiratory tract SARS-CoV-2 tissue explants zoonosis [DOI] 10.1177/03009858211073678 [Article Type] Review
JAK inhibitor blocks COVID-19 cytokine-induced JAK/STAT/APOL1 signaling in glomerular cells and podocytopathy in human kidney organoidsJAK 억제제는 사구체 세포에서 COVID-19 사이토카인 유도 JAK/STAT/APOL1 신호전달 및 인간 신장 오르가노이드에서 족세포병증을 차단합니다Article Published on 2022-06-082022-09-11 Journal: JCI Insight [Category] COVID19(2023년), MERS, SARS, 유전자 메커니즘, 진단, [키워드] abundantly expressed alleles APOL1 Baricitinib best block blocked capillary carried carrier cause Cell Cell Biology cells collapse controls COVID-19 COVID-19 infection cytokine Cytokines demonstrated Endothelial cell endothelial cells expression glomerular glomerular capillaries human kidney Infection inhibitor iPS cells. JAK inhibitor jak inhibitors JAK/STAT JAK/STAT pathway JAK/STAT signaling JAK1 kidney Kidney disease knowledge majority mechanism Nephrology nephropathy Organoid organoids pathogenic Patient phenotype podocyte Podocytes Protein reduced rescued resulting risk SARS-CoV-2 Signaling STAT Support Therapeutic benefit therapeutic benefits Trigger viability [DOI] 10.1172/jci.insight.157432 PMC 바로가기 [Article Type] Article
A diabetic milieu increases ACE2 expression and cellular susceptibility to SARS-CoV-2 infections in human kidney organoids and patient cells당뇨병 환경은 인간 신장 오르가노이드 및 환자 세포에서 SARS-CoV-2 감염에 대한 ACE2 발현 및 세포 감수성을 증가시킵니다Article Published on 2022-06-072022-09-11 Journal: Cell Metabolism [Category] COVID19(2023년), SARS, 치료기술, 치료제, [키워드] ACE2 ACE2 expression aerobic aerobic glycolysis angiotensin Angiotensin-converting enzyme angiotensin-converting enzyme 2 biopsy Cell cells cellular condition COVID-19 COVID-19 pathogenesis Critical develop diabete diabetes diabetes 2 Diabetic diabetic kidney diabetic patient diabetic patients dichloroacetate door energy metabolism enzyme exhibited Glycolysis hallmark human kidney human kidney organoids. increase individual inhibitor Intervention kidney Kidney disease kidney organoid kidney organoids metabolism mitochondrial Organoid organoids Patient prevented programming reduced resulting SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 infections severe COVID-19 susceptibility Viral detection Viral load [DOI] 10.1016/j.cmet.2022.04.009 PMC 바로가기 [Article Type] Article
Protease inhibitor Camostat Mesyalte blocks wild type SARS-CoV-2 and D614G viral entry in human engineered miniature lungs프로테아제 억제제 Camostat Mesyalte는 인간이 조작한 소형 폐에서 야생형 SARS-CoV-2 및 D614G 바이러스 진입을 차단합니다Article Published on 2022-06-012022-09-11 Journal: Biomaterials [Category] Coronavirus, COVID19(2023년), SARS, 치료제, [키워드] 3D model alveolar alveolar epithelial cell AT2 block camostat catastrophic cell line cell lines COVID-19 Culture D614G develop distal Effect epithelial epithelial cells evaluate Ex vivo highlight human lung in vitro model induce Infection inhibitor involved lentivirus lung lung epithelium maturation moderate organ organ culture organoids pandemic Pathogenesis pathogenesis of COVID-19 phenotype platform pluripotent stem cell Prevent progression Protease inhibitor provided pseudotyped real time reduced Research respiratory tract SARS-CoV-2 SARS-CoV-2 pandemic significantly the SARS-CoV-2 tissue treat type 2 alveolar epithelial cells viral entry viral infection viral infections virulent while wild type wildtype [DOI] 10.1016/j.biomaterials.2022.121509 PMC 바로가기 [Article Type] Article
Ex vivo and in vivo suppression of SARS-CoV-2 with combinatorial AAV/RNAi expression vectors조합 AAV/RNAi 발현 벡터를 사용한 SARS-CoV-2의 생체외 및 생체내 억제Article Published on 2022-05-042022-09-11 Journal: Molecular therapy : the journal of the American So [Category] COVID19(2023년), SARS, 변종, 치료제, [키워드] AAV acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 addition adeno-associated virus Antiviral Antiviral agents antiviral RNAi Cell clinically cocktail complement coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 disease symptom effective escape mutant escape mutants experiment expression vector flexible Genetic global effort Gut hACE2 in vivo infected cell infected cells intestinal cells intranasal mice mutational escape. N gene N genes organoids pandemic prevented RdRP RNA RNAi SARS virus SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 RdRp SARS-CoV-2 strains severe acute respiratory syndrome Coronavirus suppressed the SARS-CoV-2 therapeutic translation triggers Vaccine vector vectors viral adaptation Viral load viral loads virus wild-type [DOI] 10.1016/j.ymthe.2022.01.024 PMC 바로가기 [Article Type] Article
Potential SARS-CoV-2 kidney infection and paths to injury잠재적인 SARS-CoV-2 신장 감염 및 부상 경로Comment Published on 2022-05-012022-09-11 Journal: Nature reviews. Nephrology [Category] COVID19(2023년), SARS, 변종, [키워드] Autopsies Autopsy fibrosis human kidney Injury kidney infection organoids Pathways patients with COVID-19 Potential pro-fibrotic pro-inflammatory SARS-CoV-2 SARS-COV-2 infection upregulate [DOI] 10.1038/s41581-022-00551-6 PMC 바로가기 [Article Type] Comment
Protocols for SARS-CoV-2 infection in primary ocular cells and eye organoidsArticle Published on 2022-04-222023-07-09 Journal: STAR protocols [Category] COVID19(2023년), [키워드] Health Sciences immunology Microbiology organoids stem cells [DOI] 10.1016/j.xpro.2022.101383 PMC 바로가기
The Human Nose Organoid Respiratory Virus Model: an Ex Vivo Human Challenge Model To Study Respiratory Syncytial Virus (RSV) and Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Pathogenesis and Evaluate TherapeuticsResearch Article Published on 2022-02-152022-10-29 Journal: mBio [Category] COVID-19, MERS, [키워드] acute respiratory syndrome Administered Air-liquid interface air-liquid interface (ALI) culture airway Airway organoids airways ALI cultures basolateral compartment challenge cilia ciliary ciliated cell circulation coronavirus coronavirus 2 Culture Cytokines Efficacy epithelium evaluated event Ex vivo feasibility generate Human human airway human respiratory virus human respiratory viruses immunoprophylaxis immunotherapies Infants Infection innate immune responses invasive lung organoid Model Mucus noninvasive technique nose nose organoids occurred Organoid organoids palivizumab Pathogenesis Patient predict Prevent prevented respiratory respiratory syncytial virus respiratory syncytial virus (RSV) respiratory viral respiratory virus RSV SARS-CoV-2 SARS-COV-2 infection severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Therapeutics unmet need viral infection viral shedding [DOI] 10.1128/mbio.03511-21 PMC 바로가기 [Article Type] Research Article
Human Organoids and Organs‐on‐Chips for Addressing COVID‐19 ChallengesReview Published on 2022-02-022023-06-15 Journal: Advanced Science [Category] COVID19(2023년), MERS, SARS, [키워드] Coronavirus disease 2019 human models in vitro organoids organs‐on‐chips severe acute respiratory syndrome coronavirus 2 [DOI] 10.1002/advs.202105187 PMC 바로가기 [Article Type] Review