GENERATING RESPONSES IMMUNE IN CELLULAR AND HUMORAL TREATMENT WITH EPITOPE SPIKE, EPITOPE ENVELOPE PROTEIN, AND EPITOPE MEMBRANE PROTEIN SARS-COV-2, HONEY, SAUSSUREA LAPPA , AND NIGELLA SATIVAArticle Published on 2021-09-012022-10-27 Journal: African Journal of Infectious Diseases [Category] COVID-19, [키워드] Sausseria lappa administration animal model Cellular immune response cellular immunity clinical trial COVID-19 CPE CTL domination envelope protein epitope evaluated four group four groups Honey humoral immune response Humoral immunity IgG IL-4 immunomodulator Indonesia lung marker material membrane protein mice Nigella Sativa NK cell NK cells organ pandemic PBS Placebo Research Respons immune Spike protein β-defensin [DOI] 10.21010/ajidv15i2S.3 PMC 바로가기 [Article Type] Article
Processing Hundreds of SARS-CoV-2 Samples with an In-House PCR-Based Method without Robotics로봇 공학이없는 사내 PCR 기반 방법으로 수백 개의 SARS-COV-2 샘플을 처리Article Published on 2021-08-282022-09-01 Journal: Viruses [Category] MERS, SARS, 진단, 치료기술, [키워드] Accuracy buffer clinic COVID-19 infection COVID-19 infections COVID-19 pandemic COVID-19 testing detect diagnostic protocol digestion DNA human RNase P gene Internal control magnetic bead RNA extraction MONITOR multiple testing NaCl PBS PCR test per day processing protocol qPCR required reverse transcription RNA extraction RNase P robotics RT-qPCR Sample SARS-CoV-2 SARS-CoV-2 pandemic SARS-CoV-2 PCR detection sensitivity TaqMan chemistry tested Viral viral infection [DOI] 10.3390/v13091712 PMC 바로가기 [Article Type] Article
Single‐dose immunisation with a multimerised SARS‐CoV‐2 receptor binding domain (RBD) induces an enhanced and protective response in mice다량체화된 SARS-CoV-2 수용체 결합 도메인(RBD)을 사용한 단일 용량 면역은 마우스에서 강화된 보호 반응을 유도합니다Research Letter Published on 2021-08-222022-09-12 Journal: Febs Letters [Category] MERS, 진단, [키워드] Analysis Antibody titre Antigen antigens Bacteria buffer caused complexes coronavirus COVID‐19 COVID‐19 defined demonstrated Dps elicited exclusion experiment expressed form health emergency highlight immunisation increase induce introduced lung mammalian cells mice Microscopy monomeric RBD neutralising antibody pandemic Particle PBS preparation Protective purified Quality control RBD Receptor binding domain SARS‐CoV‐2 SARS‐CoV‐2 infection SARS‐CoV‐2 SDS spike Subunit vaccine triggered Vaccine viral clearance were expressed [DOI] 10.1002/1873-3468.14171 PMC 바로가기 [Article Type] Research Letter
Protective Effects of Astodrimer Sodium 1% Nasal Spray Formulation against SARS-CoV-2 Nasal Challenge in K18-hACE2 MiceK18-HACE2 마우스에서 SARS-COV-2 코 챌린지에 대한 아스 토드리머 나트륨 1% 코 스프레이 제제의 보호 효과Article Published on 2021-08-202022-08-31 Journal: Viruses [Category] MERS, SARS, 치료기술, 치료제, [키워드] administration animal animal model animals anti-SARS-CoV-2 Antiviral astodrimer blocked Brain challenge chemokine COVID-19 Cytokines Day dendrimer detectable Effect Efficacy Formulation hACE2 IL-1 IL-1α IL-1β IL-6 Incubation infected intranasally Infection Infectious virus intranasally K18-hACE2 K18-hACE2 mice lack liver lung MCP-1 nasal Nasal cavity Nasal spray neutralisation pathogen PBS Post-infection pro-inflammatory cytokine pro-inflammatory cytokines product PROTECT reduced reducing reduction reduction in SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 replication secretion serum severity of COVID-19 significantly sodium SPL7013 spray Strategy TGFβ These data TNFα Trachea treated Treatment undetectable Viraemia Viral viral genome viral replication viral shedding virus virus replication virus transmission was used were infected [DOI] 10.3390/v13081656 PMC 바로가기 [Article Type] Article
Immunogenicity of Low-Dose Prime-Boost Vaccination of mRNA Vaccine CV07050101 in Non-Human PrimatesArticle Published on 2021-08-192022-10-28 Journal: Viruses [Category] COVID-19, SARS, [키워드] acute respiratory syndrome animal models antibody approved binding boost candidate Cellular immune response cellular response coronavirus COVID COVID-19 CureVac ENCODE etiological agent humoral immune response immunogenicity injected intramuscular vaccination investigated mRNA mRNA vaccine neutralizing antibody NHP non-human primate non-human primates PBS platform pre-fusion primate Protein SARS-CoV-2 SARS-CoV-2 S shown T cell response vaccination Vaccine vaccine candidate Vaccines volunteers was performed [DOI] 10.3390/v13081645 PMC 바로가기 [Article Type] Article
Environmental stability of porcine respiratory coronavirus in aquatic environmentsResearch Article Published on 2021-07-142022-10-28 Journal: PLoS ONE [Category] COVID-19, [키워드] accumulate added aquatic environment aquatic environments Cell culture common cold Concentration conducted Contact coronavirus CoV COVID-19 CoVs droplet experiment faster filtered human disease human-to-human transmission inactivation Interaction MERS microbial Middle East North Sea nucleases PBS Proteases respiratory Respiratory Coronavirus responsible SARS-CoV-2 Scheldt Spread stability TCID temperature terrestrial testicle transmission of SARS-CoV-2 Treatment viral inactivation Viral load virus virus titre [DOI] 10.1371/journal.pone.0254540 PMC 바로가기 [Article Type] Research Article
Development of flexible electrochemical impedance spectroscopy-based biosensing platform for rapid screening of SARS-CoV-2 inhibitorsSARS-CoV-2 억제제의 신속한 스크리닝을 위한 유연한 전기화학 임피던스 분광기 기반 바이오센싱 플랫폼 개발Article Published on 2021-07-012022-09-11 Journal: Biosensors & bioelectronics [Category] SARS, 치료기술, [키워드] ACE2 ACE2 binding ACE2-SARS CoV 2 S-Protein interaction acute respiratory syndrome acute respiratory syndrome coronavirus angiotensin Angiotensin-converting enzyme angiotensin-converting enzyme 2 attenuate binding binding affinity biosensor Cell cell surface Concentration coronavirus coronavirus disease Coronavirus disease 2019 CoV COVID-19 demonstrated detect development electrochemical impedance spectroscopy (EIS) flexible in vitro include inhibitor inhibitors Interference oral cavity palladium Palladium nano-thin-film electrode PBS platform progression pyrrole PYRROLE-2-CARBOXYLIC ACID reduced S-protein SARS CoV SARS-CoV-2 SARS-CoV-2 cell SARS-COV-2 infection SARS-CoV-2 infection inhibitors. severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 Spike protein the binding affinity Volume [DOI] 10.1016/j.bios.2021.113213 PMC 바로가기 [Article Type] Article
Rapid and unamplified identification of COVID-19 with morpholino-modified graphene field-effect transistor nanosensor모르폴리노 변형 그래핀 전계 효과 트랜지스터 나노센서로 COVID-19의 신속하고 증폭되지 않은 식별Article Published on 2021-07-012022-09-11 Journal: Biosensors & bioelectronics [Category] SARS, 치료기술, [키워드] amplification Amplification-free AuNP clinical samples complementary COVID-19 COVID-19 diagnosis COVID-19 patient detection develop Fast response field-effect transistor gold Gold nanoparticle Graphene limit of detection PBS platform probe provide Rapid rapid response RdRP RNA RNA extract RNA extracts RT-PCR SARS-CoV SARS-CoV-2 SARS-CoV-2 RdRp SARS-CoV-2 RNA sensor serum specimens the healthy throat swab zone [DOI] 10.1016/j.bios.2021.113206 PMC 바로가기 [Article Type] Article
Ivermectin reduces in vivo coronavirus infection in a mouse experimental modelArticle Published on 2021-03-302022-10-04 Journal: Scientific Reports [Category] 신약개발, 치료제, [키워드] alanine aminotransferase Aspartate aminotransferase control group coronavirus diseases Coronavirus infection demonstrated disease Diseases Effectiveness euthanized general health status Health status hepatocellular histopathological in vivo infection levels infiltration Inflammatory Ivermectin liver Liver damage MHV MHV-A59 mice mouse hepatitis virus Necrosis PBS reduce RNA coronavirus SARS-CoV-2 serum transaminase significant difference significantly lower Signs and symptoms single dose therapy tissue treated Treatment viral infection Viral load were infected [DOI] 10.1038/s41598-021-86679-0 PMC 바로가기 [Article Type] Article
Detection of SARS-CoV-2 in fecal samples with different pretreatment methods and PCR kits다양한 전처리 방법 및 PCR 키트로 대변 샘플에서 SARS-CoV-2 검출Research Article Published on 2021-02-192022-09-10 Journal: BMC Microbiology [Category] MERS, 치료기술, [키워드] adjusted asymptomatic infection best can be used choice correlated COVID-19 COVID-19 patient COVID-19 patients detection detection rate distribution fecal Fecal sample fecal samples Fecal–oral transmission Fecal–oral transmission Gastrointestinal gastrointestinal symptoms groups had no inactivation inactive lead less N gene no significant difference nucleic acid test ORF1 ORF1ab PBS PCR phosphate Phosphate Buffered Saline positive rate real-time RT-PCR reported Result RT-PCR RT-PCR diagnostic kits RT-PCR kits Safe Saline SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 nucleic acid SARS-CoV-2 RNA Severe case severe cases severity severity of illness significant difference supplementary material Symptom the disease Trizol was obtained was related while [DOI] 10.1186/s12866-021-02118-0 PMC 바로가기 [Article Type] Research Article