Plaque assay – Page 3 – Communicable Diseases Research

Improving human coronavirus OC43 (HCoV-OC43) research comparability in studies using HCoV-OC43 as a surrogate for SARS-CoV-2

Article

Published on 2021-10-092022-10-04
Journal: Journal of virological methods [Category] MERS, SARS, 치료기술, 치료제,

Scalable, effective, and rapid decontamination of SARS-CoV-2 contaminated N95 respirators using germicidal ultraviolet C (UVC) irradiation device

생식신 자외 C (UVC) 조사 장치를 사용한 SARS-COV-2 오염 된 N95 호흡기의 확장 가능하고 효과적이며 빠른 오염 제거

Article

Published on 2021-10-072022-09-10
Journal: Scientific Reports [Category] MERS, SARS, 치료기술,

[키워드] Antimicrobials average biosafety COVID-19 pandemic decontamination Disease prevention Diseases distance dose effective exposure times facilitate germicidal inactivation Infectious diseases Infectious virus information inoculated irradiation LAMP lentivirus lentiviruses Log reduction measure Microbiology Microbiology techniques N95 respirator N95 respirators Other outcome Particulate Personal protective equipment Plaque assay plaque assays plaque-forming unit plaque-forming units positioning PPE protocol provide public health Respirator Respiratory tract diseases reuse Safe SARS-CoV-2 side Support supporting information tested These data titration ultraviolet virus
[DOI] 10.1038/s41598-021-99431-5 PMC 바로가기 [Article Type] Article

Discrepancies in Infectivity of Flavivirus and SARS-CoV-2 Clinical Samples: An Improved Assay for Infectious Virus Shedding and Viremia Assessment

플라비바이러스와 SARS-CoV-2 임상 샘플의 감염력 불일치: 감염성 바이러스 배출 및 바이러스혈증 평가를 위한 개선된 분석

Article

Published on 2021-09-182022-09-10
Journal: International Journal of Environmental Research an [Category] SARS, 치료기술,

[키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 addition alternative Antibody titers assessment Avicel Cellulose clinical clinical sample clinical samples coronavirus COVID-19 culture medium Dengue evaluated flavivirus Improved infectious particles Infectivity infectivity assessment Laboratory MCC medium methylcellulose microcrystalline microcrystalline cellulose Neutralizing antibody titer neutralizing antibody titers nucleic acid nucleic acid amplification nucleic acid amplification tests overlay medium Particle plaque Plaque assay plaques respiratory SARS-CoV-2 SARS-CoV-2 strains sensitivity severe acute respiratory syndrome Coronavirus shedding significantly time-consuming Viremia virus virus infectivity virus strains virus titer virus titers Virus titration was performed was used while
[DOI] 10.3390/ijerph18189845 PMC 바로가기 [Article Type] Article

Antiviral Activity of Umifenovir In Vitro against a Broad Spectrum of Coronaviruses, Including the Novel SARS-CoV-2 Virus

Article

Published on 2021-08-232022-10-28
Journal: Viruses [Category] COVID-19, SARS,

Paradoxical effects of cigarette smoke and COPD on SARS-CoV-2 infection and disease

SARS-COV-2 감염 및 질병에 대한 담배 연기 및 COPD의 역설적 영향

Research

Published on 2021-08-232022-09-10
Journal: BMC Pulmonary Medicine [Category] Fulltext, MERS, SARS, 치료법,

[키워드] ACE2 ACE2 protein acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Affect airway airways alveolar alveolar epithelial cell bronchial epithelial cell C57BL/6 Calu Calu-3 cell calu-3 cells chronic obstructive chronic obstructive pulmonary disease cigarette smoke Control controls COPD coronavirus coronavirus 2 determined by disease double-stranded RNA dsRNA Effect ELISA entry receptor epithelia epithelial cells evaluated expression of SARS-CoV-2 HBECs human bronchial epithelial cells immunofluorescence immunofluorescence staining immunostaining in vitro In vitro infection in vivo independent Infection inhibited investigated mice mRNA level mRNA levels N protein obstructive pulmonary disease Patient Plaque assay pulmonary disease qPCR receptor ACE2 replicative respiratory Result RT-qPCR SARS-CoV-2 entry receptor SARS-COV-2 infection SARS-CoV-2 replication SARS-CoV2 Severe acute respiratory syndrome severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 severity staining supplementary material TMPRSS2 Treatment Viral viral replication western blot
[DOI] 10.1186/s12890-021-01639-8 PMC 바로가기 [Article Type] Research

SARS-CoV-2 Proteins Bind to Hemoglobin and Its Metabolites

SARS-CoV-2 단백질은 헤모글로빈과 그 대사산물에 결합합니다

Article

Published on 2021-08-212022-09-10
Journal: International Journal of Molecular Sciences [Category] MERS, SARS, 유전자 메커니즘,

[키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 addition Adsorption affinity column Analysis analyzed approaches bind binding caused coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 domain explain FIVE hematological heme hemin hemoglobin identify in silico analysis in vitro inhibitory effect Interaction M metabolite Microarray motif motifs N N protein N-terminal domain Nsp3 Nsp7 NTD nucleoprotein peptide peptides Plaque assay Protein protein–protein binding Proteins proteomic Proteomic analysis proteomics protoporphyrin RBD RBD region receptor recruit recruitment Replication respiratory S protein SARS-CoV-2 SARS-CoV-2 proteins SARS-CoV-2 spike sequence severe acute respiratory syndrome Coronavirus spike Spike protein suppressed the N protein the RBD the S protein the spike protein these compound these compounds Vero cells Viral viral replication virus was used
[DOI] 10.3390/ijms22169035 PMC 바로가기 [Article Type] Article

Prolonged viral shedding of SARS-CoV-2 in two immunocompromised patients, a case report

두 명의 면역 저하 환자에서 SARS-COV-2의 장기간 바이러스 흘림, 사례 보고서

Case Reports

Published on 2021-08-032022-09-01
Journal: BMC Infectious Diseases [Category] MERS, SARS, 진단,

[키워드] acute respiratory syndrome Admission Analysis blood sample Blood samples Case report CD8+ T cell cellular Chain Reaction Clinical course clinical courses collected coronavirus coronavirus 2 coronavirus disease Coronavirus disease 2019 courses COVID-19 Culture Dexamethasone during admission first positive flow cytometry full genome sequencing Genome sequencing Hospitalization Humoral immunity ICU immune marker immune markers Immunocompromised Immunocompromised host Immunocompromised patient Immunocompromised patients immunological infected with SARS-CoV-2 intensive care intensive care unit Isolation marker one patient Patient PCR test Plaque assay polymerase chain polymerase chain reaction Prolonged reactive real time Remdesivir respiratory respiratory symptoms reverse transcription reverse transcription polymerase chain reaction RT-PCR RT-PCR test SARS-CoV-2 SARS-CoV-2 IgG severe acute respiratory syndrome coronavirus 2 severe respiratory symptom severe respiratory symptoms supplementary material T cells Treatment undetectable Viral viral culture Viral load viral shedding virus Virus shedding without symptom
[DOI] 10.1186/s12879-021-06429-5 PMC 바로가기 [Article Type] Case Reports

Lack of active SARS-CoV-2 virus in a subset of PCR-positive COVID-19 congregate care patients

PCR 양성 COVID-19 집단 치료 환자의 하위 집합에서 활성 SARS-CoV-2 바이러스 부족

Article

Published on 2021-08-012022-09-11
Journal: Journal of Clinical Virology [Category] SARS, 치료기술,

[키워드] Accuracy antibody Antibody titer Care collected correlated COVID-19 Culture cultures detect diagnosis of COVID-19 disease genomic incidence interferon interferon signaling log NAAT Nasopharyngeal swab nasopharyngeal swab sample Nasopharyngeal swab samples non-infectious not affect nucleic acid nucleic acid amplification nucleic acid amplification tests occurred pathway Patient patients PCR-positive plaque Plaque assay plaque-forming unit positive qPCR quantitation RNA fragment RT-qPCR SARS-CoV-2 SARS-CoV-2 RNA SARS-CoV-2 virus signaling pathway Stage Standard of care Stat1 Sub-genomic RNA subset symptom onset tested Vero Vero E6 Vero E6 cell VERO E6 cells Vero STAT1(−/−). Viral RNA viral RNA load virus
[DOI] 10.1016/j.jcv.2021.104879 PMC 바로가기 [Article Type] Article

D614G Substitution of SARS-CoV-2 Spike Protein Increases Syncytium Formation and Virus Titer via Enhanced Furin-Mediated Spike Cleavage

SARS-CoV-2 스파이크 단백질의 D614G 대체는 강화된 푸린 매개 스파이크 절단을 통해 융합체 형성 및 바이러스 역가를 증가시킵니다

Research Article

Published on 2021-07-272022-09-10
Journal: mBio [Category] MERS, SARS, 변종, 유전자 메커니즘,

[키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 analyzed Cell cleavage coronavirus D614G D614G substitution enhanced enhancement expressing S protein Formation furin furin cleavage site furin inhibitor increase isolates mechanism Mutation Plaque assay promote Protein rapid expansion respiratory S protein S proteins SARS-CoV-2 SARS-CoV-2 strain SARS-CoV-2 strains severe acute respiratory syndrome Coronavirus significantly higher spike Substitution syncytium syncytium formation the S protein titer Transmissibility Treatment variant Viral viral isolate virus titer
[DOI] 10.1128/mBio.00587-21 PMC 바로가기 [Article Type] Research Article

Atomic force microscopy analysis of native infectious and inactivated SARS-CoV-2 virions

원자력 현미경의 원자력 및 불 활성화 된 SARS-COV-2 비리 온의 원자력 현미경 분석

Article

Published on 2021-06-042022-09-01
Journal: Scientific Reports [Category] MERS, SARS, 진단,