Next-Generation Sequencing and Proteomics of Cerebrospinal Fluid From COVID-19 Patients With Neurological Manifestations신경학적 징후가 있는 COVID-19 환자의 뇌척수액의 차세대 시퀀싱 및 단백질체학Immunology Published on 2021-12-092022-09-11 Journal: Frontiers in Immunology [Category] SARS, 변종, 유전자 메커니즘, 치료기술, [키워드] Ageusia alterations Analysis Anosmia Case series case sery cellular cerebrospinal fluid changes in CNS complement complement system conducted Control COVID-19 COVID-19 confirmed patients COVID-19 patient CSF detection rate Diagnosis differentially expressed difficulty feature headache Infection Interaction Macrophage macrophages manifestation neurological Neurological involvement neurological manifestation neurological manifestations Next-generation sequencing NGS non-COVID-19 patient outbreak patch Pathogenesis pathological mechanism pathway Patient patients PI3K PI3K-Akt signaling Protein proteomic Proteomic analysis proteomics reported SARS-CoV-2 SARS-CoV-2 RNA screened selected Sequencing serum sample serum samples variant [DOI] 10.3389/fimmu.2021.782731 PMC 바로가기 [Article Type] Immunology
Identification of serum prognostic biomarkers of severe COVID-19 using a quantitative proteomic approach정량적 단백질 학적 접근법을 사용하여 심한 코비드 -19의 혈청 예후 바이오 마커의 확인Article Published on 2021-10-192022-08-31 Journal: Scientific Reports [Category] MERS, SARS, 바이오마커, 유전자 메커니즘, 진단, 치료법, [키워드] acquisition approach best Biomarkers Cardiovascular disorders characterized CHI3L1 Clinical prognosis COVID-19 pandemic COVID-19 patient COVID-19 patients COVID-19 prognosis cytokine cytokine signaling determine diagnostic differential expression differentially expressed ELISA assay evaluated Health help identification identify IGFALS IL-6 implicated Ingenuity Pathway Analysis interleukin introduced mass spectrometry Necrosis Patient predicted predictor Prognosis prognostic biomarker Prognostic factor Prognostic factors Prognostic markers progression Protein proteomic Proteomic analysis Quantitative regulated serum Serum protein serum proteins severe COVID-19 severe COVID-19 patients severe disease severely ill COVID-19 patients systemic inflammatory response the disease TNF Treatment Tumor tumor necrosis tumor necrosis factor viral infection with COVID-19 [DOI] 10.1038/s41598-021-98253-9 PMC 바로가기 [Article Type] Article
Dried blood sample analysis by antibody array across the total testing processArticle Published on 2021-10-152022-10-04 Journal: Scientific Reports [Category] 바이오마커, [키워드] Analysis analyzed antibody approach Assay systems Blood blood sample Cytokines database heterogeneity High-throughput screening overcome Protein proteomic Proteomic analysis proteomics provided quantitation Quantitative serum utility Volume volumetric widespread [DOI] 10.1038/s41598-021-99911-8 PMC 바로가기 [Article Type] Article
SARS-CoV-2 Coronavirus Spike Protein-Induced Apoptosis, Inflammatory, and Oxidative Stress Responses in THP-1-Like-Macrophages: Potential Role of Angiotensin-Converting Enzyme Inhibitor (Perindopril)Immunology Published on 2021-09-202022-11-01 Journal: Frontiers in Immunology [Category] COVID-19, SARS, [키워드] ACE ACE2 activated Activation Analysis analyzed angiotensin-converting enzyme 2 angiotensin-converting enzyme inhibitor Apoptosis binding Blood Cell cells changed circulation component conditioned media coronavirus CoV cytokine expression Effect Effects Endothelial cell entry of SARS-CoV-2 expression glycoprotein HUVEC cells increase in Inflammation Inflammatory marker inhibitor intracellular calcium intracellular cytokine involved Local Macrophage macrophage polarization macrophages MCP-1 MHC-II modulation monocyte/macrophages mononuclear cell oxidative stress pathway PBMC PBMCs Perindopril Peripheral blood phenotype Potential proinflammatory Proteins Proteomic analysis purified receptors reduced regulated response role ROS S protein SARS-CoV-2 sensitivity significantly increased spike Spike protein stimulated Stimulation systemically the S protein THP-1 TNFα treating Treatment upregulation Vascular viability was used [DOI] 10.3389/fimmu.2021.728896 PMC 바로가기 [Article Type] Immunology
SARS-CoV-2 Proteins Bind to Hemoglobin and Its MetabolitesSARS-CoV-2 단백질은 헤모글로빈과 그 대사산물에 결합합니다Article Published on 2021-08-212022-09-10 Journal: International Journal of Molecular Sciences [Category] MERS, SARS, 유전자 메커니즘, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 addition Adsorption affinity column Analysis analyzed approaches bind binding caused coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 domain explain FIVE hematological heme hemin hemoglobin identify in silico analysis in vitro inhibitory effect Interaction M metabolite Microarray motif motifs N N protein N-terminal domain Nsp3 Nsp7 NTD nucleoprotein peptide peptides Plaque assay Protein protein–protein binding Proteins proteomic Proteomic analysis proteomics protoporphyrin RBD RBD region receptor recruit recruitment Replication respiratory S protein SARS-CoV-2 SARS-CoV-2 proteins SARS-CoV-2 spike sequence severe acute respiratory syndrome Coronavirus spike Spike protein suppressed the N protein the RBD the S protein the spike protein these compound these compounds Vero cells Viral viral replication virus was used [DOI] 10.3390/ijms22169035 PMC 바로가기 [Article Type] Article
Systematic profiling of SARS-CoV-2-specific IgG responses elicited by an inactivated virus vaccine identifies peptides and proteins for predicting vaccination efficacyArticle Published on 2021-08-172022-10-04 Journal: Cell Discovery [Category] 바이오마커, 비임상, 진단, [키워드] Antibody Response applied BBIBP-CorV China collected convalescent correlated COVID-19 COVID-19 patient Effectiveness elicited facilitate hospitalized COVID-19 patients Humoral response identify immunized immunology inactivated individual Microarray neutralization titer Nsp7 peptide Potential biomarker profile protective mechanism Protein Proteomic analysis response SARS-CoV-2 SARS-CoV-2 vaccine SARS-CoV-2 vaccines SARS-CoV-2-specific IgG serum sample the microarray the N protein the spike protein the vaccine triggered vaccination vaccination efficacy Vaccine Vaccines virus Volunteer [DOI] 10.1038/s41421-021-00309-7 PMC 바로가기 [Article Type] Article
Proteomic Signature of Host Response to SARS-CoV-2 Infection in the Nasopharynx비인두에서 SARS-CoV-2 감염에 대한 숙주 반응의 단백질 서명Research Published on 2021-08-142022-09-10 Journal: Molecular & Cellular Proteomics : MCP [Category] 진단, 치료제, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 addition AGC, automatic gain control airway AKT Analysis antiviral property antiviral signaling asymptomatic infection caused CEP131 CFAP57 characterized ciliary component components confirmed COVID-19 case controls coronavirus coronavirus disease Coronavirus disease 2019 Coronavirus infections COVID-19 COVID-19 severity COVID-19, Coronavirus disease 2019 CXCL10 downregulation dysregulated immune response dysregulation E3 ubiquitin ligases FDR, false discovery rate Health High-resolution host response humans IFN, interferon immune response implicated infected individual infected individuals Infection Inflammatory response interferon interferon-stimulated gene interferon-stimulated genes investigator investigators involved ISG15 ISGs, interferon-stimulated genes mechanism MERS-CoV, Middle East respiratory syndrome coronavirus molecular MPB, mobile phase B multiorgan disease MX1 nasopharyngeal swabs negative control negative controls NHBE, normal human bronchial epithelial novel NP, nasopharyngeal OGFR, opioid growth factor receptor OPRPN, Opiorphin prepropeptide pandemic Pathogenesis pathogenesis of COVID-19 Pathways performed phosphoproteome Phosphoproteomics Phosphorylation PKC PPIase, peptidyl-prolyl cis/trans isomerase PRM, parallel reaction monitoring proteasomal Protein protein synthesis proteomic Proteomic analysis proteomics PRRs, pathogen-recognition receptors respiratory response RIG-I SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2, Severe acute respiratory syndrome coronavirus 2 severe acute respiratory syndrome Coronavirus shown signaling pathway signature Stat1 subunits Swab These data upper airway upregulated upregulation valuable resource Viral viral entry viral infection viral infections virus [DOI] 10.1016/j.mcpro.2021.100134 PMC 바로가기 [Article Type] Research
O -glycosylation pattern of the SARS-CoV-2 spike protein reveals an “O-Follow-N” ruleLetter to the Editor Published on 2021-08-022022-11-01 Journal: Cell Research [Category] COVID-19, MERS, SARS, [키워드] glycosylation O -glycosylation Proteomic analysis reveal Spike protein the SARS-CoV-2 [DOI] 10.1038/s41422-021-00545-2 PMC 바로가기 [Article Type] Letter to the Editor
Type 2 Diabetes Coagulopathy Proteins May Conflict With Biomarkers Reflective of COVID-19 Severity제2형 당뇨병 응고병증 단백질은 COVID-19 심각성을 반영하는 바이오마커와 충돌할 수 있습니다Endocrinology Published on 2021-06-252022-09-11 Journal: Frontiers in Endocrinology [Category] MERS, SARS, 바이오마커, 유전자 메커니즘, [키워드] Analysis aptamer baseline Biomarker Biomarkers blood glucose change changed changes in clinical Coagulation coagulation cascade coagulation factor Cohort cohort of patient complement condition conflict Control controls COVID-19 COVID-19 disease COVID-19 severity Critical degranulation demonstrated disease disease severity euglycemia factor IX Glucose heparin hypoglycemia induced Kininogen neutrophil normoglycemia objective other conditions patients Platelet predict Protein proteomic Proteomic analysis proteomics Registration Result severity of COVID-19 slow suggested T2D Thrombospondin Type type 2 diabetes Vitamin vitamin K was performed were measured [DOI] 10.3389/fendo.2021.658304 PMC 바로가기 [Article Type] Endocrinology
Multianalyte serology in home-sampled blood enables an unbiased assessment of the immune response against SARS-CoV-2가정에서 채취한 혈액의 다중 분석물 혈청학은 SARS-CoV-2에 대한 면역 반응의 편견 없는 평가를 가능하게 합니다.Article Published on 2021-06-172022-09-12 Journal: Nature Communications [Category] MERS, SARS, 진단, [키워드] 95% CI accumulated antibody approach Asymptomatic biomedical engineering Blood collected consequence coronavirus COVID-19 pandemic develop Diagnostic markers Diversity dried blood spot Dried blood spots Epidemiology healthcare IgG IgG and IgM IgM immune response include individual Influenza influenza-like symptom Influenza-like symptoms Proteomic analysis random RBD S protein SARS-CoV-2 SARS-CoV-2 protein SARS-CoV-2 proteins Screen Serological testing serology seropositive Seroprevalence spring the S protein Viral proteins [DOI] 10.1038/s41467-021-23893-4 PMC 바로가기 [Article Type] Article