A protease-activatable luminescent biosensor and reporter cell line for authentic SARS-CoV-2 infection진정한 SARS-CoV-2 감염을 위한 프로테아제 활성화 발광 바이오센서 및 리포터 세포주Research Article Published on 2022-02-102022-09-10 Journal: PLoS Pathogens [Category] COVID19(2023년), MERS, SARS, 변종, 진단, 치료기술, [키워드] 384-well plate activate Activation antibody approaches can be used Cell cell line cleavage cleave COVID-19 demonstrated detect enzyme expressing expression faster generate gold gold standard infected cells Infection infection with SARS-CoV-2 Infectious virus Laboratory lack linkers luciferase neutralisation Neutralising Antibodies neutralising antibody number of infected optical plaque plaque assays protease Proteases PROTECT Protein quantitation released Replication reporter reporter cell reporter cells required SARS-CoV-2 SARS-COV-2 infection serological signal technique these viruses time-consuming titration to define utility variants variants of concern variants of SARS-CoV-2 Viral viral infection virus viruses [DOI] 10.1371/journal.ppat.1010265 PMC 바로가기 [Article Type] Research Article
Development and multi-center clinical trials of an up-converting phosphor technology-based point-of-care (UPT-POCT) assay for rapid COVID-19 diagnosis and prediction of protective effects신속한 CovID-19 진단 및 보호 효과의 예측을위한 UPT-POCT (Up-Converting Phosphor Technology-CARE) 분석의 개발 및 다중 센터 임상 시험Clinical Trial Published on 2022-02-032022-09-01 Journal: BMC Microbiology [Category] COVID19(2023년), Fulltext, MERS, SARS, 임상, 진단, 치료기술, 치료제, [키워드] Accuracy antibody Chain Reaction China clinical trial clinically Concentration correlation COVID-19 COVID-19 cases COVID-19 diagnosis COVID-19 patient COVID-19 patients Critical cross-reaction cross-reactions Cutoff defined development ELISA evaluated healthy hospital inactivated indicated intensity lateral flow lateral flow strip microneutralization Microneutralization assay NAb titer NAb titers Neutralizing activity neutralizing antibody OD value OD values Patient performed POCT point-of-care Polymerase chain reaction test positive protective effect Protective effects quantitation Quantitative RBD RBD-specific antibodies RBD-specific antibody RBD-specific total antibodies Reactions Receptor-binding domain recovered patient recovered patients Result reveal reverse transcriptase SARS-CoV-2 SARS-CoV-2 spike protein Sensitivity and specificity serum sample serum samples Seven significantly specific antibodies Spike protein supplementary material test results the antibody the receptor-binding domain total antibodies total antibody Up-conversion phosphor technology vaccinee vaccinees viruses was determined [DOI] 10.1186/s12866-022-02450-z PMC 바로가기 [Article Type] Clinical Trial
Omicron variant showed lower neutralizing sensitivity than other SARS-CoV-2 variants to immune sera elicited by vaccines after boost오미크론 변이체는 부스트 후 백신에 의해 유도된 면역 혈청에 대해 다른 SARS-CoV-2 변이체보다 낮은 중화 민감도를 나타냈습니다.Coronaviruses Published on 2022-01-242022-09-10 Journal: Emerging Microbes & Infections [Category] COVID19(2023년), SARS, 변종, 진단, [키워드] B.1.1.529 BBIBP-CorV Beta boost booster booster vaccination COVID-19 Delta dose elicited escaped GMT GMTs Heterologous heterologous protein homologous homologous or heterologous immune Immune escape immune protection immunogenicity IMPROVE inactivated Inactivated vaccine Inactivated Vaccines limit multiple mutations neutralization neutralization ability neutralization activity Neutralizing neutralizing antibody omicron patients positive protein subunit vaccine prototype pseudotype Pseudotyped viruses quantitation raised reduction reported SARS-CoV-2 SARS-CoV-2 variant sensitivity sera significantly increased Subunit vaccine titre Vaccine variant VoC VOCs ZF2001 [DOI] 10.1080/22221751.2021.2022440 PMC 바로가기 [Article Type] Coronaviruses
Absolute quantitation of individual SARS-CoV-2 RNA molecules provides a new paradigm for infection dynamics and variant differences개별 SARS-CoV-2 RNA 분자의 절대 정량은 감염 역학 및 변이체 차이에 대한 새로운 패러다임을 제공합니다Research Article Published on 2022-01-202022-09-10 Journal: eLife [Category] SARS, 변종, 유전자 메커니즘, 치료기술, [키워드] B.1.1.7 B.1.1.7 variant cell population cells cellular clinical implications contributing to COVID-19 Degradation detection efficiency displaying early replication early stage early stages effort event exhibit genomes genomic RNA Human Infection mechanism nucleases positive provide quantification quantitation Replication replication kinetics Research RNA genome SARS-CoV-2 SARS-CoV-2 replication SARS-CoV-2 RNA molecule significantly single-molecule fluorescence in situ hybridisation smFISH subgenomic RNAs Transcription Transmissibility Transmission variant variant of concern Victoria Viral Viral proteins viral replication Viral RNA viral RNAs viral variant viruses [DOI] 10.7554/eLife.74153 PMC 바로가기 [Article Type] Research Article
Quantitation of Trastuzumab and an Antibody to SARS-CoV-2 in Minutes Using Affinity Membranes in 96-Well Plates96웰 플레이트에서 친화성 막을 사용하여 몇 분 안에 SARS-CoV-2에 대한 Trastuzumab 및 항체 정량화Article Published on 2022-01-182022-09-12 Journal: Analytical Chemistry [Category] SARS, 진단, [키워드] 96-well plate Activation affinity Analysis average binding calibration curve Combination concentrations effective fluorescence intensity group human serum mAb mAbs membrane membranes microplate reader minute monoclonal antibody occur passage Patient peptide Plate Protein quantitation Research SARS-CoV-2 secondary antibody serum Spike protein the SARS-CoV-2 therapeutic Trastuzumab Treatment Variability [DOI] 10.1021/acs.analchem.1c03654 PMC 바로가기 [Article Type] Article
SARS-CoV-2 N-antigenemia in critically ill adult COVID-19 patients: Frequency and association with inflammatory and tissue-damage biomarkers중증 성인 COVID-19 환자의 SARS-CoV-2 N-항원혈증: 염증 및 조직 손상 바이오마커와의 빈도 및 연관성Observational Study Published on 2022-01-012022-09-11 Journal: Journal of medical virology [Category] SARS, 바이오마커, 임상, 치료기술, 치료법, [키워드] 95% confidence interval absence adult COVID-19 patient antigenemia appear association Biomarker Biomarkers C-reactive protein Chain Reaction Clinical outcome Clinical severity Cohort collected COVID-19 COVID-19 patient Critically ill D-dimer ferritin ICU patient ICU Patients immunochromatography increased mortality Inflammation inflammation biomarkers Inflammatory Lactose lateral flow Logistic regression analysis median median age moderate Mortality Odds ratio parameter patients plasma Plasma levels polymerase chain polymerase chain reaction positive quantified quantitation recruited returned RNA RNA loads RT-PCR SARS-CoV-2 SARS-CoV-2 N protein SARS-CoV-2 N-antigenemia SARS-CoV-2 nucleocapsid SARS-CoV-2 RNA SARS-CoV-2 RNAemia Serum level serum levels significantly higher specimen symptoms onset tracheal aspirate Tracheal aspirates Univariate logistic regression analysis Viral Virological were measured were used yielding [DOI] 10.1002/jmv.27300 PMC 바로가기 [Article Type] Observational Study
Gravity-Driven Microfluidic Siphons: Fluidic Characterization and Application to Quantitative ImmunoassaysArticle Published on 2021-12-022022-10-28 Journal: ACS Sensors [Category] COVID-19, [키워드] amplified anti-SARS-CoV-2 IgG antigen detection application approach automated biological sample biosensor Capillaries characterization dead volume demonstrated Dengue fever dengue NS1 dengue virus dependent on Diagnosis diagnostic ELISA enzyme-linked immunosorbent fiber IgM immunoassay immunoassays immunochromatographic intrinsically lab lateral flow membrane microfluidic overlap POC point-of-care porous porous membrane portable ELISA public health quantitation Quantitative reagent sandwich ELISA sequence serotype serotype-specific serum samples siphon smartphone diagnostics unique [DOI] 10.1021/acssensors.1c01524 PMC 바로가기 [Article Type] Article
Gravity-Driven Microfluidic Siphons: Fluidic Characterization and Application to Quantitative ImmunoassaysArticle Published on 2021-12-022022-10-28 Journal: ACS Sensors [Category] Coronavirus, Fulltext, SARS, [키워드] amplified anti-SARS-CoV-2 IgG antigen detection application approach automated biological sample biosensor Capillaries characterization dead volume demonstrated Dengue fever dengue NS1 dengue virus dependent on Diagnosis diagnostic ELISA enzyme-linked immunosorbent fiber IgM immunoassay immunoassays immunochromatographic intrinsically lab lateral flow membrane microfluidic overlap POC point-of-care porous porous membrane portable ELISA public health quantitation Quantitative reagent sandwich ELISA sequence serotype serotype-specific serum samples siphon smartphone diagnostics unique [DOI] 10.1021/acssensors.1c01524 PMC 바로가기 [Article Type] Article
Gravity-Driven Microfluidic Siphons: Fluidic Characterization and Application to Quantitative Immunoassays중력 중심 미세 유체 사이펀 : 양적 면역 분석에 대한 유체 특성 및 적용Article Published on 2021-12-022022-10-27 Journal: ACS Sensors [Category] Coronavirus, 진단, 치료기술, [키워드] a glass amplified anti-SARS-CoV-2 IgG antigen detection application approach automated biological sample biological samples biosensor Capillaries characterization dead volume demonstrated Dengue Dengue fever dengue NS1 dengue virus dependent on Device Diagnosis diagnostic Dipstick ELISA enzyme-linked immunosorbent enzyme-linked immunosorbent assay fiber glass Glass fiber gravity IgM immunoassay immunoassays immunochromatographic intrinsically lab lateral flow membrane microfluidic operation overlap POC point-of-care porous porous membrane portable ELISA public health quantitation Quantitative reagent sandwich ELISA sequence serotype serotype-specific serum samples siphon smartphone diagnostics strip unique [DOI] 10.1021/acssensors.1c01524 PMC 바로가기 [Article Type] Article
Integrated immunovirological profiling validates plasma SARS-CoV-2 RNA as an early predictor of COVID-19 mortalityResearch Article Published on 2021-11-262022-10-28 Journal: Science Advances [Category] COVID-19, [키워드] Accuracy acute respiratory syndrome adjusted hazard ratio age and sex antibody-dependent cellular cytotoxicity assessment benefit Cohort cohorts collected coronavirus COVID-19 COVID-19 mortality cytokine death description disease elevated feature help heterogeneity identify Immunoglobulin independent individuals log-transformed management marker Mortality on mechanical ventilation outcome Patient performed plasma Predictive predictor quantitation Receptor binding remained robust SARS-CoV-2 SARS-CoV-2 RNA symptom onset Therapies tissue injury Viral RNA vRNA [DOI] 10.1126/sciadv.abj5629 PMC 바로가기 [Article Type] Research Article