Immunoassays and Mass Spectrometry for Determination of Protein Concentrations in Genetically Modified Cropsarticle Published on 2024-04-122024-09-05 Journal: Journal of agricultural and food chemistry [Category] update2024, [키워드] GM crops immunoassay LC−MS/MS mass spectrometry protein concentration quantification quantitation [DOI] 10.1021/acs.jafc.3c09188 PMC 바로가기 [Article Type] article
Technical advancement and practical considerations of LC-MS/MS-based methods for host cell protein identification and quantitation to support process developmentReview Published on 2023-05-222024-09-05 Journal: mAbs [Category] update2024, [키워드] Host cell protein (HCP) identification Impurities liquid chromatography tandem mass spectrometry (LC-MS/MS) Process development quantitation [DOI] 10.1080/19420862.2023.2213365 PMC 바로가기 [Article Type] Review
Clinical Utility of Quantitative HBV Core Antibodies for Solving Diagnostic DilemmasReview Published on 2023-01-282023-06-11 Journal: Viruses [Category] B형 간염, [키워드] clinical utility Diagnostic method HBV core antibody Hepatitis B virus (HBV) new biomarker qAnti-HBc quantitation [DOI] 10.3390/v15020373 PMC 바로가기 [Article Type] Review
Standardization of SARS-CoV-2 Cycle Threshold Values: Multisite Investigation Evaluating Viral Quantitation across Multiple Commercial COVID-19 Detection Platformsarticle Published on 2023-01-182024-09-05 Journal: Microbiology Spectrum [Category] update2024, [키워드] harmonization quantitation SARS-CoV-2 Standardization Viral load [DOI] 10.1128/spectrum.04470-22 PMC 바로가기 [Article Type] article
Development and Validation of an Innovative Analytical Approach for the Quantitation of Tris(Hydroxymethyl)Aminomethane (TRIS) in Pharmaceutical Formulations by Liquid Chromatography Tandem Mass SpectrometryArticle Published on 2022-12-222023-07-11 Journal: Molecules [Category] COVID19(2023년), [키워드] COVID-19 vaccine derivatization GMP isobutyl chloroformate LC-MS Pharmaceuticals quantitation tris tromethamine validation [DOI] 10.3390/molecules28010073 PMC 바로가기
Harmonization of SARS-CoV-2 reverse transcription quantitative PCR tests to the first WHO international standard for SARS-CoV-2 RNASARS-CoV-2 RNA에 대한 최초의 WHO 국제 표준에 SARS-CoV-2 역전사 정량 PCR 테스트의 조화Article Published on 2022-09-012022-09-11 Journal: Journal of Clinical Virology [Category] COVID19(2023년), SARS, 유전자 메커니즘, 치료법, [키워드] 95% CI 95% confidence interval Analysis assays association calibration Cepheid clinical samples comparable confidence Ct value Ct values cycle cycle threshold Emergency use evaluate Generalizability generate greater harmonization IMPROVE International international standard interquartile range interval IQR log mean difference median nucleocapsid platform positive quantitation Quantitative quantitative PCR Range Regression reverse transcription RT-qPCR RT-qPCRs SARS-CoV-2 SARS-CoV-2 RNA Standard target targets the mean the WHO threshold upper respiratory viral burden was tested WHO [DOI] 10.1016/j.jcv.2022.105242 PMC 바로가기 [Article Type] Article
Equipment-free, gold nanoparticle based semiquantitative assay of SARS-CoV-2-S1RBD IgG from fingertip blood: A practical strategy for on-site measurement of COVID-19 antibodies손끝 혈액에서 SARS-CoV-2-S1RBD IgG의 장비가 필요 없는 금 나노입자 기반 반정량적 분석: COVID-19 항체의 현장 측정을 위한 실용적인 전략Article Published on 2022-08-152022-09-11 Journal: Talanta [Category] COVID19(2023년), SARS, 진단, 치료기술, [키워드] antibodies antibody AuNR Colorimetric reagent complementary correlated COVID-19 COVID-19 antibody declined ELISA assay estimation fingertip Fingertip blood gold Gold nanoparticle IgG IgG antibody Immunoglobulin Immunoglobulin G implementation management pandemic plate reader provide quantitation RBD Receptor binding domain revaccination SARS-CoV-2 semi-quantitative Serum neutralizing antibody SRAS-CoV-2 vaccination [DOI] 10.1016/j.talanta.2022.123498 PMC 바로가기 [Article Type] Article
Discovery and intranasal administration of a SARS-CoV-2 broadly acting neutralizing antibody with activity against multiple Omicron subvariantsArticle Published on 2022-08-082022-10-06 Journal: Med (New York, N.Y.) [Category] COVID19(2023년), SARS, 변종, 진단, [키워드] acting acute respiratory syndrome Administered administration antibody approved B.1.1.529 breadth cell line cGMP characterized coronavirus COVID-19 CRIPT DARPA defined diagnosed domains Emergency use FDA H2L2 mice Harbor human clinical trials human IgG1 intranasal intranasally intravenous intravenously K18-hACE2 transgenic mice LALA limit lineages Live virus lung titer NAb neutralization assays Neutralizing Neutralizing activity neutralizing antibody omicron Omicron BA.1 Omicron BA.1.1 Omicron BA.2 Pre-clinical research provided pseudotype quantitation reduced required retained SARS-CoV-2 SARS-CoV-2 neutralizing antibody SARS-CoV-2 spike subset tested therapeutic Therapies treat vaccination variant variants of concern virus weight loss [DOI] 10.1016/j.medj.2022.08.002 PMC 바로가기 [Article Type] Article
RT-qPCR and ATOPlex sequencing for the sensitive detection of SARS-CoV-2 RNA for wastewater surveillance폐수 감시를 위한 SARS-CoV-2 RNA의 민감한 검출을 위한 RT-qPCR 및 ATOPlex 시퀀싱Article Published on 2022-07-152022-09-11 Journal: Water research [Category] COVID19(2023년), SARS, 변종, 유전자 메커니즘, 치료기술, 치료제, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 appear approach assays assessment China CDC Combination Concentration Concentration method condition coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 deep sequencing detect detection Detection limit detection rate Detection sensitivity enveloped virus greater handling impacted less limit of detection MONITOR multiplex Mutation N1 and N2 nucleic acid nucleic acid extraction offer ORF1ab pandemic PCR platform powerful tool Probability promise quantitation quantitative PCR recovery required Result reverse transcription RT-qPCR RT-qPCR assay RT-qPCR assays SARS-CoV-2 SARS-CoV-2 RNA Sequencing severe acute respiratory syndrome Coronavirus shown Spread Surveillance US CDC variants variants of concern viral genome virus VoC wastewater [DOI] 10.1016/j.watres.2022.118621 PMC 바로가기 [Article Type] Article
Rapid self-test of unprocessed viruses of SARS-CoV-2 and its variants in saliva by portable wireless graphene biosensor휴대용 무선 그래핀 바이오센서를 통한 타액 내 처리되지 않은 SARS-CoV-2 바이러스 및 그 변이체의 신속한 자가 테스트Article Published on 2022-07-122022-09-11 Journal: Proceedings of the National Academy of Sciences of [Category] COVID19(2023년), SARS, 변종, 치료기술, 치료법, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus aptamer biosensor clinically Cognate Concentration coronavirus COVID-19 D614G detect determined by diagnostic DNA domain early phase environmental monitoring epidemiological epidemiologically field-effect transistor Graphene limit limit of detection LoQ MERS Middle East Middle East respiratory syndrome N protein N501Y nucleocapsid omicron pandemic plaque plaque-forming unit platform positive Precision Protein Proteins public health quantitation Rapid RBD Receptor-binding domain respiratory RT-PCR S protein Saliva saliva samples SARS-CoV-2 SARS-CoV-2 variant sensitivity sensor severe acute respiratory syndrome coronavirus 2 significantly lower specificity Spike protein statistically variant variants of concern viral particle Viral particles Viral proteins virus Y453F [DOI] 10.1073/pnas.2206521119 PMC 바로가기 [Article Type] Article