Identification of the RNase-binding site of SARS-CoV-2 RNA for anchor primer-PCR detection of viral loading in 306 COVID-19 patients306명의 COVID-19 환자에서 앵커 프라이머-PCR 검출을 위한 SARS-CoV-2 RNA의 RNase 결합 부위 식별Multicenter Study Published on 2021-03-222022-09-11 Journal: Briefings in Bioinformatics [Category] SARS, 치료기술, 치료제, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Analysis anchor primer bioinformatics Bioinformatics analysis bioinformatics analysis. Chain Reaction confirmed case confirmed cases convalescent coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 COVID-19 patient COVID-19 patients Cycle threshold value demonstrated Diagnosis feces first positive Gender identification IMPROVE male molecular Molecular diagnosis N gene nasal nuclear Older age ORF ORF gene pandemic Pharyngeal swab polymerase chain polymerase chain reaction primer reveal RNA RNase SARS-CoV-2 SARS-CoV-2 RNA sensitivity severe acute respiratory syndrome Coronavirus sputum stability supported Urine Viral RNA [DOI] 10.1093/bib/bbaa193 PMC 바로가기 [Article Type] Multicenter Study
Host Defence RNases as Antiviral Agents against Enveloped Single Stranded RNA Viruses외피 단일 가닥 RNA 바이러스에 대한 항바이러스제로서의 숙주 방어 RNaseReview Published on 2021-03-042022-09-10 Journal: Virulence [Category] SARS, 치료법, [키워드] agent antiviral activity antiviral drugs apoptosis pathways approved coronavirus coronavirus disease COVID-19 Defense develop drug drug candidate Drug development effective Enveloped single-stranded RNA Viruses evade focus Host host defense immune immune modulation immune regulation include information innate immune system Innate immunity outbreak pandemic Pathogens Pathways Perspective Prevent Recognition reported RNA RNase RNases Safe single-stranded ssRNA Stress treat unique Viral viral genome viral infection viral infections viral pathogens virus virus-host interplay viruses [DOI] 10.1080/21505594.2021.1871823 PMC 바로가기 [Article Type] Review
RNase in the saliva can affect the detection of severe acute respiratory syndrome coronavirus 2 by real-time one-step polymerase chain reaction using saliva samplesShort Communication Published on 2021-02-162023-06-16 Journal: Pathology, research and practice [Category] MERS, SARS, [키워드] COVID-19 PCR RNase Saliva SARS-CoV-2 [DOI] 10.1016/j.prp.2021.153381 PMC 바로가기 [Article Type] Short Communication
Feasibility of using alternative swabs and storage solutions for paired SARS-CoV-2 detection and microbiome analysis in the hospital environmentResearch Published on 2021-01-222022-10-29 Journal: Microbiome [Category] COVID-19, [키워드] 16S rRNA gene Abstract analyses Analysis Antibiotics collected COVID COVID-19 COVID-19 testing demonstrated detect Determining diagnostic driven by evaluated experiment FIVE global health Government hospital ICU impacted in viral include indicated infections initiative intensive care Laboratory lack limit of detection microbial community Microbiome nasal Particles Patient patients performed public health Result RNA RNase RNases RT-qPCR SARS-CoV-2 SARS-CoV-2 detection Sequencing supplementary material Swab swabs tested the patient transmission of SARS-CoV-2 Transport media transported Viral particles virus VTM was measured [DOI] 10.1186/s40168-020-00960-4 PMC 바로가기 [Article Type] Research
Comparison of Subgenomic and Total RNA in SARS-CoV-2-Challenged Rhesus MacaquesVaccines and Antiviral Agents Published on 2021-01-202022-10-30 Journal: Journal of Virology [Category] COVID-19, [키워드] administration anatomical antiviral drugs cell entry convalescent Critical Developing Efficacy genomic RNA gRNA in vivo inactivated infected cell Infection lysates macaque marker MONITOR monoclonal antibodies neutralized non-human primates nucleocapsid positive Prophylactic Protective public health remained replicating virus respiratory respiratory virus reverse transcription PCR rhesus macaque rhesus macaques RNA RNase RT-PCR RT-PCR assay SARS-CoV-2 SARS-CoV-2 virus sgRNA subgenomic RNA the SARS-CoV-2 virus therapeutic therapeutic efficacy Total total RNA utility Vaccines Viral load virions virus [DOI] 10.1128/JVI.02370-20 PMC 바로가기 [Article Type] Vaccines and Antiviral Agents
Efficient SARS-CoV-2 detection in unextracted oro-nasopharyngeal specimens by rRT-PCR with the Seegene Allplex™ 2019-nCoV assaySeegene Allplex™ 2019-nCoV assay로 rRT-PCR을 통해 추출되지 않은 구강비인두 검체에서 SARS-CoV-2를 효율적으로 검출Methodology Published on 2020-12-182022-09-01 Journal: Virology Journal [Category] MERS, SARS, 진단, 치료기술, [키워드] 2019-nCoV assay addition amplified automated can not clinical laboratories COVID-19 COVID-19 pandemic cycle demonstrated detect detectable detection detection rate Efficiency efficient False negative Laboratory N gene nasopharyngeal swabs negative sample offer PCR testing performed positive sample proteinase Proteinase K reagent reduction Result RNA RNA extraction RNase RNase-free water rRT-PCR rRT-PCR testing SARS-CoV-2 SARS-CoV-2 detection SARS-CoV-2 RNA sensitivity specimen specimens Surveillance Swab swabs thermal threshold value Treatment Universal UTM Viral viral gene viral genes Viral load was increased water [DOI] 10.1186/s12985-020-01468-x PMC 바로가기 [Article Type] Methodology
Combination Therapy Using Inhalable GapmeR and Recombinant ACE2 for COVID-19Molecular Biosciences Published on 2020-08-072022-10-31 Journal: Frontiers in Molecular Biosciences [Category] COVID-19, [키워드] ACE2 acute respiratory syndrome angiotensin-converting enzyme 2 cells clinical trials Combination coronavirus COVID-19 COVID-19 (2019-nCoV) COVID-19 patient COVID-19 patients effective Efficacy enzyme evaluated Extracellular frontline healthcare worker gapmer glycoprotein host cells lung alveolar nasal delivery Perspective Prophylactic Protein recombinant recombinant ACE2 RNase SARS-CoV-2 single-stranded DNA Therapeutic approach therapy Treatment viral infectivity Viral RNA [DOI] 10.3389/fmolb.2020.00197 PMC 바로가기 [Article Type] Molecular Biosciences
Pneumococcal RNase R globally impacts protein synthesis by regulating the amount of actively translating ribosomesarticle Published on 2019-02-282024-09-01 Journal: RNA Biology [Category] 폐렴구균 감염증, [키워드] 70S EF-G IF3 protein synthesis ribosome ribosome dissociation RNase RRF translation [Article Type] article
A novel mechanism of RNase L inhibition: Theiler’s virus L* protein prevents 2-5A from binding to RNase LResearch Article Published on 2018-04-132022-10-29 Journal: PLoS Pathogens [Category] Coronavirus, [키워드] accumulate activate activating Activation Antiviral antiviral response Apoptosis ATP bind binding Cell cellular Central nervous system chimeric contributing to Coronaviruses degrade double-stranded RNA effective enzyme expressing flu IFN in vivo inactive infected cell infected cells Infection inhibit interferon killing mechanism MHV mouse hepatitis virus murine OAS oligomerization pathway Pathways Prevent produced promote Protein Replication RNase these cell triggering upstream Viral protein Viral RNA virus virus replication virus spread [DOI] 10.1371/journal.ppat.1006989 PMC 바로가기 [Article Type] Research Article
Bovine milk RNases modulate pro-inflammatory responses induced by nucleic acids in cultured immune and epithelial cellsarticle Published on 2017-03-312024-09-01 Journal: Developmental & Comparative Immunology [Category] 살모넬라증, [키워드] Bovine PBMCs host defence immune modulation Macrophage mastitis RNase [Article Type] article