COVID-19 active case findings based on self-collected saliva samples with CRISPR-Cas12a detectionArticle Published on 2022-07-012023-07-09 Journal: Experimental Biology and Medicine [Category] COVID19(2023년), [키워드] active case findings COVID-19 CRISPR-Cas12a qRT-PCR RPA saliva samples [DOI] 10.1177/15353702221090181 PMC 바로가기
Photocontrolled crRNA activation enables robust CRISPR-Cas12a diagnosticsArticle Published on 2022-06-282023-07-09 Journal: Proceedings of the National Academy of Sciences of [Category] COVID19(2023년), [키워드] CRISPR-Cas12a Nucleic acid detection one-pot assay. PC linker RPA [DOI] 10.1073/pnas.2202034119 PMC 바로가기
Advances in nucleic acid amplification techniques (NAATs): COVID-19 point-of-care diagnostics as an exampleReview article Published on 2022-06-012022-10-05 Journal: Biosensors & bioelectronics [Category] COVID19(2023년), SARS, 진단, 치료기술, [키워드] advance amplification Bio-detection chain Characteristics Circle clinical settings Combination COVID-19 COVID-19 pandemic Diagnosis diagnostics Efficiency example feature greater limitations Molecular diagnosis NAAT nucleic acid amplification Nucleic acid amplification technologies offer PCR POCT point-of-care point-of-care diagnostics RCA reaction REcombinase Research Rolling RPA SARS-CoV-2 sensitivity Sensitivity and specificity specificity stability unique [DOI] 10.1016/j.bios.2022.114109 [Article Type] Review article
CASCADE: Naked eye-detection of SARS-CoV-2 using Cas13a and gold nanoparticlesCASCADE: Cas13a와 금 나노입자를 이용한 SARS-CoV-2 육안 탐지Article Published on 2022-05-012022-09-11 Journal: Analytica chimica acta [Category] COVID19(2023년), MERS, SARS, 진단, 치료기술, 치료법, 치료제, [키워드] acid activate aggregation approach approaches cascade cleave clinical sample clinical samples Concentration COVID-19 pandemic CRISPR CRISPR/Cas13 detect Diagnosis diagnostics Diseases effort gold Gold nanoparticle Gold nanoparticles Infectious diseases isothermal isothermal amplification molecular Naked-eye nanomaterials NASBA nasopharyngeal swabs nucleic acid nucleic acid amplification nucleic acids oligonucleotide Pathogens point-of-care Prevent Protein RNA RNase RPA SARS-CoV-2 SARS-CoV-2 RNA Scientific community sensitivity Spread ssRNA variety Viral viral nucleic acids Viral RNA [DOI] 10.1016/j.aca.2022.339749 PMC 바로가기 [Article Type] Article
A one-pot CRISPR/Cas13a-based contamination-free biosensor for low-cost and rapid nucleic acid diagnostics저렴하고 신속한 핵산 진단을 위한 원팟 CRISPR/Cas13a 기반 무오염 바이오센서Article Published on 2022-04-152022-09-11 Journal: Biosensors & bioelectronics [Category] COVID19(2023년), SARS, 치료기술, [키워드] 2019 coronavirus disease acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus-2 African amplicon contamination amplification automated carried caused centrifugation Chain Reaction cleavage Complete completion Contamination Contamination-free detection coronavirus disease Coronavirus-2 COVID-19 CRISPR CRISPR/Cas13a diagnostic diagnostic testing diagnostics Diseases DNA virus effective Epidemic equipment Fever hydrophobic independent Infectious diseases Inner lid limit of detection Low-cost detection meet negative and positive nucleic acid Nucleic acid detection Nucleic acid diagnostics One-pot reaction. outbreak pandemic PCR polymerase chain polymerase chain reaction public health Quantitative raised Rapid REcombinase recombinase polymerase amplification reliability required RNA virus RPA SARS-CoV-2 shaking spreading Treatment tube vessel virus were used [DOI] 10.1016/j.bios.2022.113994 PMC 바로가기 [Article Type] Article
Rational Design of CRISPR/Cas12a-RPA Based One-Pot COVID-19 Detection with Design of Experiments실험 계획을 통한 CRISPR/Cas12a-RPA 기반 One-Pot COVID-19 탐지의 합리적 설계Article Published on 2022-04-152022-09-11 Journal: ACS synthetic biology [Category] COVID19(2023년), SARS, 진단, 치료기술, [키워드] accelerate addition amplification applied approach benefit buffer complex component components condition COVID-19 COVID-19 pandemic CRISPR CRISPR/Cas12a critical impact definitive screening design demonstrated design of experiments detection Detection sensitivity diagnostic Diagnostic method diagnostics DNA DNA sequence DoE Effect effective experiment Factor full-length SARS-CoV-2 Genome highlight isothermal molecular Molecular diagnosis N gene one-pot COVID-19 testing optimization polymerase reaction reaction optimization Reactions recombinase polymerase amplification recombinase polymerase amplification (RPA). reverse transcription RNA RNase RNase inhibitor robust RPA RT-qPCR sensitivity significant effect significantly simple statistical variables [DOI] 10.1021/acssynbio.1c00617 PMC 바로가기 [Article Type] Article
Combining recombinase polymerase amplification and DNA-templated reaction for SARS-CoV-2 sensing with dual fluorescence and lateral flow assay outputSARS-CoV-2 감지를 위한 재조합 효소 중합효소 증폭 및 DNA 주형 반응을 이중 형광 및 측면 흐름 분석 출력과 결합Article Published on 2022-04-012022-09-11 Journal: Biopolymers [Category] COVID19(2023년), SARS, 치료기술, [키워드] absence acid acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 amplification applied Biotin chain Chain Reaction coronavirus coumarin diagnostic disease detection DNA DNA templated reaction early disease early phase effective exacerbated Isolation lateral flow lateral flow assay magnitude methodology naked eye nucleic acid nucleic acid sensing. pandemic Patient peptide PNA point-of-care polymerase polymerase chain polymerase chain reaction reaction recombinase polymerase amplification RNA sequence RPA SARS-CoV-2 severe acute respiratory syndrome Coronavirus stimulated the SARS-CoV-2 transfer Transmission Vaccines while [DOI] 10.1002/bip.23485 PMC 바로가기 [Article Type] Article
An integrated rapid nucleic acid detection assay based on recombinant polymerase amplification for SARS-CoV-2SARS-CoV-2에 대한 재조합 중합효소 증폭을 기반으로 하는 통합된 신속한 핵산 검출 분석Article Published on 2022-02-012022-09-11 Journal: Virologica Sinica [Category] COVID19(2023년), SARS, 치료기술, [키워드] amplification cartridge cross reaction limit of detection Nucleic acid detection polymerase respiratory tract RPA SARS-CoV-2 sealed Treatment viruses [DOI] 10.1016/j.virs.2022.01.006 PMC 바로가기 [Article Type] Article
Fabrication of Wearable PDMS Device for Rapid Detection of Nucleic Acids via Recombinase Polymerase Amplification Operated by Human Body HeatArticle Published on 2022-01-272022-10-27 Journal: Biosensors [Category] COVID-19, [키워드] acid amplification amplify applied Body body heat condition conducted Contact coronavirus disease COVID-19 detection Device DNA DNA amplification DNA plasmid E. coli Escherichia coli fabrication gel electrophoresis Genomic DNA heat Human human body heat IMPROVE limit of detection Modification nucleic acid amplification pandemic Plasmid poly(dimethylsiloxane) (PDMS) polymerase Rapid REcombinase Recombinase polymerase amplification (RPA) RPA SARS-CoV-2 Support temperature wearable RPA microdevice [DOI] 10.3390/bios12020072 PMC 바로가기 [Article Type] Article
Fabrication of Wearable PDMS Device for Rapid Detection of Nucleic Acids via Recombinase Polymerase Amplification Operated by Human Body HeatArticle Published on 2022-01-272022-10-27 Journal: Biosensors [Category] Coronavirus, COVID-19, SARS, [키워드] acid amplification amplify applied Body body heat condition conducted Contact coronavirus disease COVID-19 detection Device DNA DNA amplification DNA plasmid E. coli Escherichia coli fabrication gel electrophoresis Genomic DNA heat Human human body heat IMPROVE limit of detection Modification nucleic acid amplification pandemic Plasmid poly(dimethylsiloxane) (PDMS) polymerase Rapid REcombinase Recombinase polymerase amplification (RPA) RPA SARS-CoV-2 Support temperature wearable RPA microdevice [DOI] 10.3390/bios12020072 PMC 바로가기 [Article Type] Article