Preparation and pre-clinical evaluation of flagellin-adjuvanted NOM vaccine candidate formulated with Spike protein against SARS-CoV-2 in mouse modelArticle Published on 2022-10-012022-11-15 Journal: Microbial pathogenesis [Category] COVID19(2023년), SARS, 진단, [키워드] 3D structure acute respiratory syndrome Analysis anti-S Antigen Antisera bind Cell Cell-mediated immune responses chimeric China Cluster coronavirus cytokine develop docking effective elicit ELISA ENhance evaluated expressed flagellin groups humoral IFN-γ IgG IL-10 IL-17 IL-22 IL-6 immune response immunoinformatics indicated Infection Interaction mice Ni-NTA NOM protein ORF3a outbreak performed pneumonia case preparation promoted Protein purified raised receptor S protein Salmonella SARS-CoV-2 significantly increased spike spleen TLR5 TNF-α Total vaccination Vaccine vaccine candidate vaccine candidates vaccine. was selected were assessed Wuhan [DOI] 10.1016/j.micpath.2022.105736 PMC 바로가기
SARS-CoV-2 spike protein aggregation is triggered by bacterial lipopolysaccharideArticle Published on 2022-10-012022-11-16 Journal: Febs Letters [Category] COVID19(2023년), SARS, 변종, 치료기술, [키워드] aggregate aggregation analyses assays bacterial lipopolysaccharide binding boost COVID-19 demonstrated endotoxins Evidence gel High dose Inflammation Invasion lipopolysaccharide LPS Microscopy Molecular simulation oligomers peptide predicted Protein protein-aggregation Proteins S protein SARS-CoV-2 SARS-CoV-2 spike protein sequence Spike protein spike protein. triggered virus [DOI] 10.1002/1873-3468.14490 PMC 바로가기
Immunological study of COVID-19 vaccine candidate based on recombinant spike trimer protein from different SARS-CoV-2 variants of concernArticle Published on 2022-09-292022-11-15 Journal: Frontiers in Immunology [Category] SARS, 변종, 진단, [키워드] addition AstraZeneca Beta booster booster dose CD4 CD4+ CD8+ central memory ChAdOx1 Concentration COVID-19 COVID-19 vaccine Delta evaluated follicular highest homologous IFN-gamma IgG IgG antibody IgG isotype IL-17 Immune escape immunogenic immunogenicity induce memory mice mice immunization neutralizing antibody Neutralizing antibody titer new SARS-CoV-2 performed Population populations Protein S protein SARS-CoV-2 SARS-CoV-2 S protein SARS-CoV-2 variant SARS-CoV-2 variants significantly higher spike trimer spike trimeric protein T cells tested trimeric Trimeric spike protein Vaccine vaccine candidate vaccine candidate. Vaccines variant variants viral variant virus viruses VoC Wuhan [DOI] 10.3389/fimmu.2022.1020159 PMC 바로가기
Enzyme linked oligonucleotide assay for the sensitive detection of SARS-CoV-2 variantsArticle Published on 2022-09-292022-11-15 Journal: Frontiers in Cellular and Infection Microbiology [Category] SARS, 변종, 진단, 치료기술, [키워드] antibody aptamer buffer condition conditions Delta delta variant detect detection Detection limit develop diagnostic effective elements ELONA human nasal fluid identify in vitro inactivated Modification predicted RBD reporter Research S glycoprotein S protein Sandwich SARS-CoV-2 SARS-CoV-2 RBD SARS-CoV-2 variant Screening SEM sensitivity Spike protein spread of COVID-19 stability TCID variant viral particle virus virus. was obtained was used Wuhan [DOI] 10.3389/fcimb.2022.1017542 PMC 바로가기
A computational study of metal-organic frameworks (MOFs) as potential nanostructures to combat SARS-CoV-2Article Published on 2022-09-202022-11-15 Journal: Scientific Reports [Category] SARS, 치료제, [키워드] ACE acute respiratory syndrome angiotensin-converting enzyme 2 antibody antiviral drug binding biological molecule carrier coronavirus COVID-19 Critical deformation determine drug host cells inhibitor Interaction investigated M pro MOF molecular nanobody non-structural protein protease Protein receptor S protein SARS-CoV-2 secondary structure Spike protein surface protein tested Vaccine virus replication [DOI] 10.1038/s41598-022-19845-7 PMC 바로가기
Identification and characterization of a novel cell binding and cross-reactive region on spike protein of SARS-CoV-2Article Published on 2022-09-192022-11-15 Journal: Scientific Reports [Category] SARS, 변종, 진단, [키워드] addition amino acid sequence Antisera Beas-2B cells binding Cell characterized conserved COVID-19 Cross reactivity cross-reactive develop effective vaccine Escherichia coli evade full-length Host host cell identification IgG immune imperative in viral Infection inhibited involved murine Mutation mutations neutralizing monoclonal antibody observation Prevent produced pseudovirus purified Recombinant protein S protein S-protein SARS-CoV SARS-CoV-2 Serologically Spike protein virus virus variant [DOI] 10.1038/s41598-022-19886-y PMC 바로가기
Understanding the Pharmacology of COVID-19 mRNA Vaccines: Playing Dice with the Spike?Article Published on 2022-09-172022-11-15 Journal: International Journal of Molecular Sciences [Category] SARS, 유전자 메커니즘, 진단, [키워드] adverse effects Antigen complex conventional vaccines COVID-19 COVID-19 mRNA vaccine Dice drug Effect emergency condition Evidence implication mechanism of action mRNA mRNA vaccine mRNA vaccines Pharmacodynamics pharmacokinetic pharmacokinetics Pharmacology representing resulting S protein Safe Safety Safety assessment SARS-CoV-2 SARS-CoV-2 S protein Spike protein spike protein. the SARS-CoV-2 took place translation undergo understanding vaccination [DOI] 10.3390/ijms231810881 PMC 바로가기
Isolation of a human SARS-CoV-2 neutralizing antibody from a synthetic phage library and its conversion to fluorescent biosensorsArticle Published on 2022-09-152022-11-15 Journal: Scientific Reports [Category] SARS, 진단, 치료기술, [키워드] acute respiratory syndrome addition angiotensin-converting enzyme 2 antibody antibody fragment antigen detection binding Clinical treatment clone coronavirus demonstrated diagnose effective Fab fluorescent Health help Human monoclonal antibody human SARS-CoV-2 IgG neutralize neutralizing antibody outbreak Prevent public health S protein S1 domain SARS-CoV-2 selected spike glycoprotein spread of COVID-19 viral infection viral infections virus detection [DOI] 10.1038/s41598-022-19699-z PMC 바로가기
Intranasal administration of a single dose of MVA-based vaccine candidates against COVID-19 induced local and systemic immune responses and protects mice from a lethal SARS-CoV-2 infectionArticle Published on 2022-09-122022-11-15 Journal: Frontiers in Immunology [Category] SARS, 변종, 진단, 치료기술, [키워드] absence acute respiratory syndrome Administered administration animal model blocked bronchoalveolar C57BL/6 caused CD4 CD8 coronavirus Coronavirus disease-19 COVID-19 COVID-19 progression current demonstrated effective Efficacy elicited evaluated expressing full-length histopathological IgA antibody IgG immune responses Immunity immunogenic immunogenicity induce intramuscular intranasal intranasal delivery K18-hACE2 transgenic mice Local lung lung lesion lymph nodes mice modified vaccinia virus morbidity and mortality mucosal mucosal immune MVA nasal washe Nasal washes neutralizing antibody parental Prevent pro-inflammatory cytokine PROTECT Protective protective efficacy Protein responses Result S protein SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 transmission SARS-CoV-2 variant SARS-CoV-2 virus SARS-CoV-2-specific immune response serum single dose spleen susceptible systemic immune response systemically T-cell immune response the SARS-CoV-2 Transmission undetectable upper respiratory tract vaccination Vaccine vaccine administration vaccine candidate vaccine candidates vaccine candidates. vaccine dose virus infection [DOI] 10.3389/fimmu.2022.995235 PMC 바로가기
Dok3 restrains neutrophil production of calprotectin during TLR4 sensing of SARS-CoV-2 spike proteinArticle Published on 2022-09-122022-11-16 Journal: Frontiers in Immunology [Category] SARS, 변종, [키워드] activate blocking calprotectin circulating contribute COVID-19 Cytokine storm Delta Dok3 downstream dysregulated immune response effective elevated hallmark Increased JAK2 mechanism neutrophil Neutrophils neutrophils. omicron Prevent Protein recruit S protein SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 spike SARS-CoV-2 spike protein severe COVID-19 severe COVID-19 manifestation Signaling STAT3 susceptible patients target therapy TLR4 variant variants [DOI] 10.3389/fimmu.2022.996637 PMC 바로가기