Spectral image contrast-based flow digital nanoplasmon-metry for ultrasensitive antibody detection초고감도 항체 검출을 위한 스펙트럼 이미지 대비 기반 유동 디지털 나노플라즈몬 측정Research Published on 2022-01-042022-09-10 Journal: Journal of Nanobiotechnology [Category] SARS, 진단, 치료기술, [키워드] amplification amplified Analysis Antibody assays Antibody detection artificial saliva AuNP boundary colorimetry conducted demonstrated Digital SPR FIVE gold Gold nanoparticles immunoassay immunoassays improvement Improvements limit of detection Local Local surface plasmon resonance (LSPR) magnitude Protein provide restrict Result Saliva SARS-CoV-2 scattering selectivity sensitivity signal-to-noise ratio Spectral Spectral image contrast Spike protein Spike proteins supplementary material surface plasmon surface plasmon resonance Volume [DOI] 10.1186/s12951-021-01188-6 PMC 바로가기 [Article Type] Research
Production of full-length SARS-CoV-2 nucleocapsid protein from Escherichia coli optimized by native hydrophobic interaction chromatography hyphenated to multi-angle light scattering detection다각도 광산란 검출에 하이픈으로 연결된 천연 소수성 상호작용 크로마토그래피에 의해 최적화된 대장균으로부터 전장 SARS-CoV-2 뉴클레오캡시드 단백질 생산Article Published on 2021-12-012022-09-11 Journal: Talanta [Category] SARS, 치료기술, 치료법, 치료제, [키워드] abundantly expressed acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Analysis analytical method Cell cell lysis chaperone Chromatography complementary coronavirus cpCasp2‐based platform fusion protein process (CASPON technology). Critical diagnostic dimerized downstream Downstream processing (DSP) DSP E. coli enhanced enzyme-linked immunosorbent enzyme-linked immunosorbent assay Escherichia coli explained fermentation fraction full-length SARS-CoV-2 fusion protein host cell hydrophobic Hydrophobic interaction chromatography-multi-angle light scattering (HIC-MALS) Infection information Interaction life cycle mass spectrometry minor molecular MONITOR novel Novel coronavirus Novel coronavirus (termed SARS-CoV-2) Nuclease nucleic acids nucleocapsid protein nucleoprotein peptide platform produced Production Protein protein expression purification purified purity resolving power SARS-CoV-2 scattering SEC selectivity sequence severe acute respiratory syndrome Coronavirus target protein Treatment variable viral life cycle was used were expressed yield [DOI] 10.1016/j.talanta.2021.122691 PMC 바로가기 [Article Type] Article
Preclinical characterization of an intravenous coronavirus 3CL protease inhibitor for the potential treatment of COVID19COVID19의 잠재적인 치료를 위한 정맥 코로나바이러스 3CL 프로테아제 억제제의 전임상 특성화Article Published on 2021-10-182022-09-10 Journal: Nature Communications [Category] MERS, SARS, 변종, 신약개발, 치료제, [키워드] 3CL ADME antiviral activity broad spectrum caused clinical development clinical evaluation Combination coronavirus COVID-19 COVID19 exhibited global pandemic Human human host in vitro in vivo inhibit inhibited inhibiting intravenous mice PF-07304814 Pharmacodynamics pharmacokinetics phosphate Potential treatment Preclinical Preclinical research prodrug protease Protease activity Protease inhibitor Protein Remdesivir safety profile SARS-CoV SARS-CoV-2 SARS-CoV-2 virus selectivity Support sustained targets the SARS-CoV-2 virus Treatment [DOI] 10.1038/s41467-021-26239-2 PMC 바로가기 [Article Type] Article
Catching COVID: Engineering Peptide-Modified Surface-Enhanced Raman Spectroscopy Sensors for SARS-CoV-2코로나 잡기: SARS-CoV-2용 펩티드 수정 표면 강화 라만 분광기 센서 엔지니어링Article Published on 2021-09-242022-09-11 Journal: ACS Sensors [Category] SARS, 변종, 치료기술, [키워드] Accuracy ACE2 albumin amplification angiotensin Angiotensin-converting enzyme angiotensin-converting enzyme 2 biomimetic biosensor bovine bovine serum albumin complex COVID-19 Engineering generate globe highlighting IMPROVE limit of detection peptide peptide-based peptides probe provide quantification quantitative assay Raman spectroscopy SARS-CoV-2 selective selectivity sensor SERS serum albumin specificity Spectroscopy Spike protein the spike protein unique variants Viral protein Viral proteins virus viruses [DOI] 10.1021/acssensors.1c01344 PMC 바로가기 [Article Type] Article
Clinical Application of the Novel Cell-Based Biosensor for the Ultra-Rapid Detection of the SARS-CoV-2 S1 Spike Protein Antigen: A Practical ApproachSARS-COV-2 S1 스파이크 단백질 항원의 초경량 검출을위한 새로운 세포 기반 바이오 센서의 임상 적용 : 실용적인 접근법Comparative Study Published on 2021-07-062022-09-10 Journal: Biosensors [Category] 치료기술, [키워드] antibody Antigen antigen tests applicability application applied Asymptomatic attachment Bioelectric Recognition Assay (BERA) biosensor Cell chimeric clinical COVID-19 detect detection electric potential expressed False negative false negative result handle incidence Infection mammalian cell mass Mass screening mass surveillance membrane engineering nasopharyngeal sample novel patients plug Point-of-Care (POC) positive sample public health surveillance rapid antigen test Recognition reported RT-PCR S1 spike protein SARS-CoV-2 Screening selectivity sensitivity Serological assay severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) significantly spike Spike protein Surveillance the SARS-CoV-2 Viral Viral protein virus were recorded [DOI] 10.3390/bios11070224 PMC 바로가기 [Article Type] Comparative Study
SARS-CoV-2 Receptor Binding Domain as a Stable-Potential Target for SARS-CoV-2 Detection by Surface—Enhanced Raman Spectroscopy표면에 의한 SARS-CoV-2 검출을 위한 안정적-잠재적 표적으로서의 SARS-CoV-2 수용체 결합 도메인 - 강화된 라만 분광법Article Published on 2021-07-052022-09-10 Journal: Sensors (Basel, Switzerland) [Category] SARS, 치료기술, 치료제, [키워드] albumin antibody approach bovine serum albumin BSA Concentration detect detection Detection limit develop experiment Frequency open optical preparation Protein protein level Raman spectroscopy RBD RBD protein reported SARS-CoV-2 SARS-CoV-2 RBD protein SARS-COV-2 receptor binding domain selectivity sensitivity SERS shown silver/gold nanostructures Spectroscopy substrate substrates target the preparation virus viruses was obtained were used [DOI] 10.3390/s21134617 PMC 바로가기 [Article Type] Article
Development and Validation of a Method for Quantification of Favipiravir as COVID-19 Management in Spiked Human Plasma스파이크 인간 혈장에서 COVID-19 관리로서 Favipiravir의 정량화 방법의 개발 및 검증Article Published on 2021-06-222022-09-10 Journal: Molecules [Category] MERS, 치료제, [키워드] Accuracy acetonitrile acyclovir bio-analytical Bioanalytical Method Validation blank calibration curve Concentration concerning COVID-19 COVID-19 pandemic development dichloromethane evaluated Favipiravir flow rate Guidance heteroscedasticity HPLC Human Human plasma isocratic linear Linear regression LLOQ maximum methanol no significant difference phosphate phosphate buffer plasma Precision proportion reduced regression coefficient researcher resolved selectivity sensitivity separated solvent spiked human plasma stability US-FDA validation was obtained Weighting [DOI] 10.3390/molecules26133789 PMC 바로가기 [Article Type] Article
Challenges for Targeting SARS-CoV-2 Proteases as a Therapeutic Strategy for COVID-19COVID-19에 대한 치료 전략으로 SARS-CoV-2 프로테아제를 표적화하기 위한 과제Article Published on 2021-06-112022-09-11 Journal: ACS Infectious Diseases [Category] MERS, SARS, 진단, 치료제, [키워드] antiviral potency cathepsin cathepsin cross-reactivity Cathepsins Cell cellular challenge Compound COVID-19 cysteine cysteine protease cysteine proteases determine effective inhibitor effort evaluated explained expressing expression focus highlight host cell identify infection assays inhibited inhibitor inhibitors lead lysosomal cathepsins M pro main protease Papain Papain-like protease potency Prevent produced protease Proteases purified reported SARS-CoV-2 SARS-CoV-2 protease SARS-CoV-2 virus screened selectivity Serine Strategy targeting the SARS-CoV-2 virus TMPRSS2 transmembrane serine protease Treatment treatment of COVID-19 Viral viral entry viral entry pathway viral infectivity viral replication [DOI] 10.1021/acsinfecdis.0c00815 PMC 바로가기 [Article Type] Article
Combined recombinase polymerase amplification/rkDNA-graphene oxide probing system for detection of SARS-CoV-2SARS-CoV-2 검출을 위한 결합된 재조합 효소 중합효소 증폭/rkDNA-그래핀 옥사이드 프로빙 시스템Article Published on 2021-05-082022-09-11 Journal: Analytica chimica acta [Category] SARS, 치료기술, 치료제, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 added amplification appear Combined complementary coronavirus Diagnosis DNA amplification Enzymatic in situ synthetic probe (rkDNA) exhibited Fluorescence Graphene Graphene oxide help Infection isothermal isothermal amplification N-gene pandemic virus point-of-care polymerase Prevent probe RPA SARS-CoV-2 selective selectivity sensitivity sequence severe acute respiratory syndrome Coronavirus Spread target [DOI] 10.1016/j.aca.2021.338390 PMC 바로가기 [Article Type] Article
Development of a Highly Selective Plasmodium falciparum Proteasome Inhibitor with Anti-malaria Activity in Humanized MiceArticle Published on 2021-04-192024-08-28 Journal: Angewandte Chemie (International Ed. in English) [Category] 말라리아, [키워드] inhibitors malaria proteasome selectivity Therapeutics