Structure Based Affinity Maturation and Characterizing of SARS-CoV Antibody CR3022 against SARS-CoV-2 by Computational and Experimental ApproachesArticle Published on 2022-01-192022-10-28 Journal: Viruses [Category] COVID-19, SARS, [키워드] affinity against SARS-CoV antibody antibody affinity approach Autoimmune binding binding free energy CDRs complementarity-determining region conserved Coronaviruses COVID-19 COVID-19 epidemic CR3022 criteria cross-react cross-reactivity demonstrated drug ELISA enzyme-linked immunosorbent experiments higher affinity hydrophobic interactions IMPROVE in vitro indicate MM/PBSA molecular molecular dynamics (MD) simulation Molecular simulation mutant mutate mutated Mutation neutralizing antibody performed plasma powerful tool provided RBD reactivity Receptor-binding domain (RBD) recognizing residue salt-bridge SARS-CoV SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 RBD selected significantly SPR Structure the binding affinity Treatment wild-type [DOI] 10.3390/v14020186 PMC 바로가기 [Article Type] Article
Discovery of Highly Potent Fusion Inhibitors with Potential Pan-Coronavirus Activity That Effectively Inhibit Major COVID-19 Variants of Concern (VOCs) in Pseudovirus-Based AssaysArticle Published on 2021-12-312022-10-28 Journal: Viruses [Category] COVID-19, SARS, [키워드] activities activity acute respiratory syndrome ADME African Analysis B.1.1.7 B.1.351 B.1.617.2 bind Candidates Compound concern coronavirus COVID-19 Cytopathic effect delta variant demonstrated distribution facilitate fusion fusion inhibitor half-maximal inhibitory concentration in vivo India inhibited inhibitor inhibitors inhibitory lowest Major MERS-CoV metabolism Middle East Middle East respiratory syndrome (MERS) oral bioavailability pan-coronavirus potent Potential prefusion prevented respiratory syndrome coronavirus SARS-CoV SARS-CoV-2 SARS-CoV-2 spike protein Severe acute respiratory syndrome (SARS) small molecule South SPR surface plasmon resonance trimer variant variants of concern VOCs [DOI] 10.3390/v14010069 PMC 바로가기 [Article Type] Article
Antibody affinity maturation and cross-variant activity following SARS-CoV-2 mRNA vaccination: Impact of prior exposure and sexResearch paper Published on 2021-12-102022-11-01 Journal: EBioMedicine [Category] COVID-19, SARS, [키워드] Advancing Affinity maturation Alliance antibody antibody affinity Award binding center contribute convalescent convalescent individual convalescent individuals countermeasure COVID-19 Department described determine dose FDA female females finding funding Human Impact individual Infection Infectious disease initiative knowledge Limited male manuscript maturation mRNA mRNA vaccination mutant mutants naïve neutralization titer neutralizing antibody NIAID NIH no difference number Participants Plasmon prefusion profile Pseudovirus neutralization assay RBD Receptor binding domain resonance SARS-CoV-2 Science sera service Sex sex differences significantly higher SPR supported surface the SARS-CoV-2 university Vaccine vaccine dose variants variants of concern variants of interest Virus neutralization VOCs VOIs was used [DOI] 10.1016/j.ebiom.2021.103748 PMC 바로가기 [Article Type] Research paper
Development of a simple and miniaturized sandwich-like fluorescence polarization assay for rapid screening of SARS-CoV-2 main protease inhibitorsMethodology Published on 2021-12-052022-11-01 Journal: Cell & Bioscience [Category] COVID-19, [키워드] 3CL protease inhibitor acute respiratory syndrome Analysis antiviral agent approved BAS catalytic site caused Chinese traditional medicine conserved coronavirus coronavirus disease COVID-19 COVID-19 patients develop development Dieckol docking Ecklonia cava enzyme evaluated exhibited Fluorescence polarization genomic RNA high affinity High-throughput screening highest identify in viral in vitro inhibitor Interaction Main protease inhibitor MPro pandemic polyprotein predicted protease Proteases Replication Result SARS-CoV-2 SARS-CoV-2 main protease SARS-CoV-2 Mpro selective SPR Spread therapeutic agent therapeutic target Treatment [DOI] 10.1186/s13578-021-00720-3 PMC 바로가기 [Article Type] Methodology
Production of scFv, Fab, and IgG of CR3022 Antibodies Against SARS-CoV-2 Using Silkworm-Baculovirus Expression System누에-배큘로바이러스 발현 시스템을 이용한 SARS-CoV-2에 대한 CR3022 항체의 scFv, Fab 및 IgG 생산Article Published on 2021-12-012022-09-11 Journal: Molecular biotechnology [Category] SARS, 유전자 메커니즘, 진단, [키워드] affinity Against antibody caused COVID-19 CR3022 CR3022 antibody diagnostic diagnostic agents Emerging infectious diseases expression expression vector Fab IgG IgG antibodies IgG antibody Infectious disease Infectious diseases mammalian monoclonal antibody produced Production RBD Receptor binding domain Research S protein SAME AS SARS patients SARS-CoV SARS-CoV-2 scFv shown Silkworm-baculovirus expression vector system spike Spike protein SPR System the spike protein therapeutic agents Viral [DOI] 10.1007/s12033-021-00373-0 PMC 바로가기 [Article Type] Article
Anti-SARS-CoV-2 Activity of Rhamnan Sulfate from Monostroma nitidum모노 스트로마 니티 디움으로부터의 Rhamnan 설페이트의 항 -SARS-COV-2 활성Article Published on 2021-11-302022-08-31 Journal: Marine Drugs [Category] SARS, 변종, 치료제, [키워드] ACE2 activities activity acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 addition angiotensin Angiotensin-converting enzyme angiotensin-converting enzyme 2 antiviral activity backbone binding Characteristics competitive binding coronavirus COVID-19 COVID-19 pandemic delta variant demonstrated enzyme evaluated fucoidans glycans Health heparan sulfate heparin Host host cell host cell receptor host cells in vitro Interaction investigated monosaccharide Monostroma much lower mutant nuclear nuclear magnetic resonance Particle pathogen polysaccharide previous study Protein RBD receptor Receptor binding domain respiratory responsible rhamnan sulfate rhamnose S-protein SARS-CoV-2 SARS-CoV-2 spike protein severe acute respiratory syndrome Coronavirus Spike protein SPR sulfate sulfated surface plasmon surface plasmon resonance the SARS-CoV-2 two-dimensional variant variants Viral viral infectivity wild type [DOI] 10.3390/md19120685 PMC 바로가기 [Article Type] Article
Cross-reactivity of antibodies from non-hospitalized COVID-19 positive individuals against the native, B.1.351, B.1.617.2, and P.1 SARS-CoV-2 spike proteins근원이없는 COVID-19 긍정적 인 개체로부터의 항체의 교차 반응성, B.1.351, B.1.617.2 및 p.1 SARS-COV-2 스파이크 단백질Article Published on 2021-11-082022-09-01 Journal: Scientific Reports [Category] MERS, SARS, 변종, 진단, 치료기술, [키워드] ACE-2 ACE-2 receptor Adults affinity analyzed antibodies antibody antibody level antibody levels B.1.351 B.1.617.1 B.1.617.2 Beta BNT162b2 ChAdOx1 ChAdOx1 nCoV-19 collected COVID-19 cross-reactivity Delta ectodomain Effectiveness elicited enrolled Evidence evidence of except for Gamma humoral Humoral response implication individual individuals infected with SARS-CoV-2 inhibited Interaction interactions nCoV neutralization Optical sensors P.1 P.1 variant pandemic PCR-confirmed positive individual Proteins relative SARS-CoV-2 spike SARS-CoV-2 spike protein SARS-CoV-2 variant SARS-CoV-2 variants sera Spike protein Spike proteins SPR Spread sustained the spike protein vaccinated individual vaccinated individuals vaccination variant spike VoC VOCs [DOI] 10.1038/s41598-021-00844-z PMC 바로가기 [Article Type] Article
SARS-CoV-2 Exacerbates COVID-19 Pathology Through Activation of the Complement and Kinin SystemsSARS-CoV-2는 보체 및 키닌 시스템의 활성화를 통해 COVID-19 병리를 악화시킵니다Immunology Published on 2021-11-052022-09-11 Journal: Frontiers in Immunology [Category] MERS, SARS, 진단, 치료법, [키워드] activate Activation acute respiratory syndrome best binding blockade bradykinin C1q classical pathway complement complement system contribute COVID-19 Efficiency ELISA ENCODE generate Genome globular head Human plasma include inflammatory pathway Kallikrein Kallikrein-kinin system Kinin kinin-kallikrein system membrane molecular weight multi-organ failure normal human plasma nucleocapsid peptide peptides platform post COVID “long-haulers” Protein receptor Recombinant protein respiratory SARS-CoV-2 Severe acute respiratory syndrome solid phase SPR structural protein structural proteins surface plasmon resonance System the SARS-CoV-2 therapeutic Treatment treatment of COVID-19 Trigger triggers turn Vasoactive Viral viral particle [DOI] 10.3389/fimmu.2021.767347 PMC 바로가기 [Article Type] Immunology
Active components in Ephedra sinica stapf disrupt the interaction between ACE2 and SARS-CoV-2 RBD: Potent COVID-19 therapeutic agentsEphedra Sinica STAPF의 활성 성분은 ACE2와 SARS-COV-2 RBD 사이의 상호 작용을 방해합니다 : 강력한 COVID-19 치료제Article Published on 2021-10-052022-08-31 Journal: Journal of ethnopharmacology [Category] MERS, SARS, 치료제, [키워드] 293T-ACE2 4-hydroxy-6-methoxyquinoline-2-carboxylic acid (PubChem CID: 22017520) 4-hydroxyquinoline-2-carboxylic acid (PubChem CID: 3845) 4,6-dihydroxyquinoline-2-carboxylic acid (PubChem CID: 440752) ACE2 ACE2-RBD activity alleviate angiotensin Angiotensin-converting enzyme angiotensin-converting enzyme 2 Asia Asthma binding domain Calu calu-3 cells carboxylic acid carried cells Chinese Cold component Compound compounds cough COVID-19 COVID-19 therapeutic agent Deterioration disrupt disrupting docking domain effort ephedra Ephedra sinica Ethanol HPLC hydrogen Hydrogen bond hydroxy indicated inhibit inhibit SARS-CoV-2 inhibiting Inhibition assays inhibitory activity Interaction Isolation knock-out Lung diseases material mechanism molecular docking NMR observé potent potential therapeutic agent potential therapeutic agents PPI PPI inhibitor Prevent protein-protein interaction provided pseudoviruses PubChem quinoline Quinoline-2-carboxylic acid S protein SARS-CoV-2 SARS-CoV-2 RBD SARS-CoV-2 S SARS-CoV-2 S protein SARS-CoV-2 virus SARS-CoV-2 virus infection Spike protein SPR Structure structures suggested surface plasmon surface plasmon resonance Symptom TCM the SARS-CoV-2 therapeutic agents these compound these compounds Traditional Chinese medicine treat viropexis virus infection were used [DOI] 10.1016/j.jep.2021.114303 PMC 바로가기 [Article Type] Article
SARS-CoV-2 spike protein detection through a plasmonic D-shaped plastic optical fiber aptasensor플라즈몬 D형 플라스틱 광섬유 앱타센서를 통한 SARS-CoV-2 스파이크 단백질 검출Article Published on 2021-10-012022-09-11 Journal: Talanta [Category] SARS, 치료기술, [키워드] Analysis aptamer binding BSA characterized detection develop diagnostic domain fiber film fluorescence microscopy gold human serum Laboratory MERS MERS spike protein optical Optical fiber plastic Plastic optical fiber (POF) point-of-care Polyethyleneglycol (PEG) probe Protein Protein binding rapid response RBD Receptor-binding domain SARS-CoV-2 SARS-CoV-2 spike glycoprotein SARS-CoV-2 spike protein self Self assembled monolayer (SAM) sequence specificity spike glycoprotein Spike protein SPR surface surface plasmon surface plasmon resonance surface plasmon resonance (SPR) tested the SARS-CoV-2 [DOI] 10.1016/j.talanta.2021.122532 PMC 바로가기 [Article Type] Article