A Method To Prevent SARS-CoV-2 IgM False Positives in Gold Immunochromatography and Enzyme-Linked Immunosorbent AssaysImmunoassays Published on 2020-04-102022-10-30 Journal: Journal of Clinical Microbiology [Category] COVID-19, MERS, SARS, [키워드] acute respiratory syndrome analyzed Concentration coronavirus COVID-19 COVID-19 patient detect Diabetes Mellitus disease dissociate ELISA enzyme-linked immunosorbent enzyme-linked immunosorbent assay Factor False-positive False-positive results gold Gold immunochromatography assay HIV infection hypertensive patients IgM immunochromatography influenza A virus influenza B Legionella pneumophila Mycoplasma pneumoniae Novel coronavirus Novel coronavirus infection patients positive reactivity reducing SARS-CoV-2 SARS-CoV-2 IgM sera serum serum samples the SARS-CoV-2 urea virus were used [DOI] 10.1128/JCM.00375-20 PMC 바로가기 [Article Type] Immunoassays
Development of indirect enzyme-linked immunosorbent assay for detection of porcine epidemic diarrhea virus specific antibodies (IgG) in serum of naturally infected pigsResearch Article Published on 2019-11-122022-10-28 Journal: BMC veterinary research [Category] Coronavirus, [키워드] (PEDV) Accuracy addition Analysis antibody Antigen applied Asymptomatic AUC characteristic coated contagious Culture cut-off value develop development Diagnostic method diarrhea disease Efficacy ELISA ELISA plate ELISA test enzyme-linked immunosorbent epidemic diarrhea evaluate evaluated high mortality high mortality rate IgG Infection Infectious disease Japan Long-term preservation neutralization neutralization test Newborn occur occurred outbreak PEDV Porcine epidemic diarrhea virus positive positive correlation Pre-coated plate preserved reference method Result ROC Sensitivity and specificity sera serological survey serum serum samples specific antibody symptomatic Vero Cell virus vomiting was determined watery [DOI] 10.1186/s12917-019-2123-2 PMC 바로가기 [Article Type] Research Article
Sensitive and Specific Detection of Low-Level Antibody Responses in Mild Middle East Respiratory Syndrome Coronavirus InfectionsResearch Published on 2019-10-012022-10-31 Journal: Emerging Infectious Diseases [Category] Coronavirus, MERS, [키워드] antibodies Asymptomatic asymptomatic to mild Camels coronavirus cross-reactivity detectable detection diagnostics ELISA Human Infection lack Lung disease MERS MERS-CoV Microarray Middle East Middle East respiratory syndrome Coronavirus mild clinical symptom mild infection Neutralizing PCR-confirmed PCR-confirmed patient Prevalence Protein Qatar reported respiratory respiratory syndrome coronavirus response risk S1 sensitive sensitivity Seroconversion serology serum samples South Korea Specific spike Surveillance The Netherlands virus transmission virus-neutralizing antibodies viruses [DOI] 10.3201/eid2510.190051 PMC 바로가기 [Article Type] Research
Preliminary investigation on feline coronavirus presence in the reproductive tract of the tom cat as a potential route of viral transmissionOriginal Articles Published on 2019-03-222022-10-29 Journal: Journal of Feline Medicine and Surgery [Category] Coronavirus, MERS, [키워드] appear association Blood blood sample cattery management Contact coronavirus correlated disorder Feline coronavirus Feline infectious peritonitis histology immune-mediated disease immunohistochemistry Infection initiated male molecular objective PCR performed positive prevention Probability quantitative PCR reproductive Result reverse transcriptase PCR RT-PCR RT-qPCR Semen Semen samples Seroconversion serology serum serum sample serum samples testicle tom cat reproduction transcriptase Transmission unlikely Viral load Viral RNA Viral transmission virus was performed [DOI] 10.1177/1098612X19837114 PMC 바로가기 [Article Type] Original Articles
Development of a sensitive and specific xMAP assay for detection of antibodies against infectious laryngotracheitis and bronchitis virusesMethodology Published on 2018-09-212022-10-28 Journal: Virology Journal [Category] Coronavirus, MERS, [키워드] antibody bronchitis can be used coated with demonstrated detect detection system development differential diagnosis disease ELISA ELISA assays enzyme-linked immunosorbent exhibited glycoprotein IBV imperative incubated Infectious Bronchitis Virus Infectious bronchitis virus, antibody detection, ELISA Infectious laryngotracheitis virus Influenza virus Luminex xMAP technology microsphere multiplex nonspecific nucleocapsid Protein purified quantification reaction Result serological serum serum sample serum samples tested vaccination virus [DOI] 10.1186/s12985-018-1048-x PMC 바로가기 [Article Type] Methodology
Development of a one-step RT-PCR assay for detection of pancoronaviruses (α-, β-, γ-, and δ-coronaviruses) using newly designed degenerate primers for porcine and avian `fecal samplesArticle Published on 2018-02-272022-11-01 Journal: Journal of virological methods [Category] Coronavirus, MERS, [키워드] amplify Analysis Asia bat biological sample biological samples characterized conditions conserved Coronaviruses CoV CoVs Critical degenerate Degenerate primers demonstrated detect develop development domain ENCODE enteric diseases epidemic diarrhea Epidemics fecal gastroenteritis intestinal Laboratory measure Pancoronavirus RT-PCR pathogen PEDV Porcine deltacoronavirus positive primer Protein reported RNA RNA-dependent RNA polymerase RNA-dependent RNA polymerase (RdRP) gene RT-PCR RT-PCR assay RT-PCR assays Sensitivity and specificity sequenced sequenced samples sera serum samples tested universal primers virus [DOI] 10.1016/j.jviromet.2018.02.021 PMC 바로가기 [Article Type] Article