The Role of Human Coronaviruses in Children Hospitalized for Acute Bronchiolitis, Acute Gastroenteritis, and Febrile Seizures: A 2-Year Prospective StudyResearch Article Published on 2016-05-122022-10-28 Journal: PLoS ONE [Category] Coronavirus, [키워드] Admission age association blood sample Bronchiolitis children clinical presentation coronavirus detect disease etiological examined febrile Follow-up gastroenteritis healthy control healthy controls highest Hospitalized Human nasopharyngeal NP swab Patient polymerase positive proportion Prospective Prospective Study Registration remains uncertain role Seizure specimen specimens Stool Surgical Procedure Swab variety virus viruses was used [DOI] 10.1371/journal.pone.0155555 PMC 바로가기 [Article Type] Research Article
Reverse Transcription Cross-Priming Amplification–Nucleic Acid Test Strip for Rapid Detection of Porcine Epidemic Diarrhea VirusArticle Published on 2016-04-192022-10-29 Journal: Scientific Reports [Category] Coronavirus, [키워드] acid amplification amplification product applied binding antibody cause conducted contribute coronavirus detection Detection limit detection system disease effective epidemic diarrhea evaluated N gene Neonatal nucleic acid test offer PCR PCR method PEDV Plasmid primer probe Rapid Result reverse transcription RNA sensitivity sequence specimens strip target gene Test virus were used [DOI] 10.1038/srep24702 PMC 바로가기 [Article Type] Article
Molecular characterization of human coronaviruses and their circulation dynamics in Kenya, 2009–2012Research Published on 2016-02-012022-10-28 Journal: Virology Journal [Category] Coronavirus, MERS, [키워드] Acute respiratory infection all age group amplification Analysis analyzed cells characterized children circulation diagnosed distribution eight Evidence FIVE HCoV-229E HCoV-HKU1 HCoV-NL63 HCoV-OC43 Hospitalization Human human coronavirus human coronaviruses identity Immunity individual Infection information inoculated isolate Kenya MERS-CoV molecular molecular characterization monolayer morbidity nasopharyngeal specimen polymerase positive Prevalence Primers real time Respiratory illness responsible Result RT-PCR sequence homology Sequencing shown specimen specimens strain Strains Variation virus [DOI] 10.1186/s12985-016-0474-x PMC 바로가기 [Article Type] Research
Development of a real-time reverse transcription loop-mediated isothermal amplification method for the rapid detection of porcine epidemic diarrhea virusResearch Published on 2015-05-142022-10-28 Journal: Virology Journal [Category] Coronavirus, MERS, [키워드] characterized clinical sample clinical samples Clinical studies contagious detect detection Detection limit develop development diarrhea disease epidemic diarrhea equipment isothermal amplification Laboratory less outbreak PEDV real-time RT-PCR Result reverse transcription RNA RT-LAMP RT-PCR sensitivity specimens the epidemic virus viruses vomiting watery [DOI] 10.1186/s12985-015-0297-1 PMC 바로가기 [Article Type] Research
Development of loop-mediated isothermal amplification assay for detection of human coronavirus-NL63Article Published on 2011-04-272022-11-01 Journal: Journal of virological methods [Category] Coronavirus, [키워드] Analysis cell culture supernatant conserved coronavirus cross-react Detection limit develop development diagnostic distribution found Genome HCoV-NL63 Human isothermal amplification Laboratory LAMP Loop-mediated isothermal amplification motif Netherlands NL63 nucleic acid Other respiratory viruses pathogen PCR Prevalence Real-time PCR RNA specimens technique [DOI] 10.1016/j.jviromet.2011.04.024 PMC 바로가기 [Article Type] Article
Detection of novel respiratory viruses from influenza‐like illness in the Philippines †Research Article Published on 2010-04-192022-11-01 Journal: Journal of medical virology [Category] Coronavirus, [키워드] collected coronavirus country detect detected detection Epidemiology HKU1 human bocavirus human metapneumovirus Inc KI virus Melaka virus métapneumovirus nasopharyngeal aspirate NL63 not found novel virus Patient PCR performed polymerase chain Respiratory samples respiratory virus respiratory viruses RT‐PCR specimens subtype Transcription tropical virus WU virus [DOI] 10.1002/jmv.21763 PMC 바로가기 [Article Type] Research Article
Detection of four human coronaviruses in respiratory infections in children: A one‐year study in ColoradoResearch Article Published on 2009-07-222022-11-01 Journal: Journal of medical virology [Category] Coronavirus, [키워드] assay assays association children CNS coronavirus death detected detection diagnostic diarrhea disease Diseases Epidemiology evaluate Evidence healthy children Hospitalized human coronavirus illness Immunocompromised patient Inc Infection Laboratory Lower Lower respiratory tract majority occurred Patient patients PCR polymerase chain positive positive patient raise respiratory respiratory infection respiratory specimen respiratory tract infection respiratory virus rhinovirus RNA RT‐PCR Seizures Seven specimen specimens submitted tested vomiting [DOI] 10.1002/jmv.21541 PMC 바로가기 [Article Type] Research Article
Coronavirus HKU1 Infection in the United StatesResearch Published on 2006-05-012022-10-31 Journal: Emerging Infectious Diseases [Category] Coronavirus, [키워드] Adenovirus age Analysis children circulating Clinical features collected coronavirus Coronavirus HKU1 Coronaviruses disease Evidence HCoV-HKU1 HKU1 Hong Kong Infection Influenza virus Lower respiratory tract parainfluenza positive respiratory infections respiratory specimen respiratory syncytial virus respiratory tract screened specimens tested the United State Two patient viruses [DOI] 10.3201/eid1205.051316 PMC 바로가기 [Article Type] Research
Evidence of human coronavirus HKU1 and human bocavirus in Australian childrenArticle Published on 2005-10-272022-11-01 Journal: Journal of Clinical Virology [Category] Coronavirus, [키워드] absence Acute respiratory tract disease aetiology age applied assay children compared contribute contributing to coronavirus described diagnoses diagnostic disease Epidemiology evidence of found Genetic HCoV-HKU1 HKU1 human bocavirus human coronavirus incidence infectious Infectious disease instance Lineage Lower respiratory tract microbe microbial molecular occur Patient PCR assay reductions in respiratory tract risk of infection significantly specimens strain virus viruses [DOI] 10.1016/j.jcv.2005.09.008 PMC 바로가기 [Article Type] Article
Rapid and sensitive detection of multiple genes from the SARS‐Coronavirus using quantitative RT‐PCR with dual systemsResearch Article Published on 2005-08-242022-11-01 Journal: Journal of medical virology [Category] Coronavirus, [키워드] 2003 ABI accurate acute respiratory syndrome addition Applied Biosystem caused Cell center comparable Control coronavirus cross demonstrated described detect detection detection efficiency Diagnosis evaluated examined Gene human sera in vitro Inc Infection isolate membrane nucleocapsid other coronavirus outbreak PCR prevention primer Prism Quantitative Rapid reagent real‐time quantitative RT‐PCR relative sensitivity RNA Roche RT‐PCR Safe SARS SARS patients SARS‐CoV structural genes SARS2 sensitivity sequence specimen specimens System TaqMan assay transcribed Viral RNA virus viruses [DOI] 10.1002/jmv.20432 PMC 바로가기 [Article Type] Research Article