A novel LC-MS/MS method for determination of the potential antiviral candidate favipiravir for the emergency treatment of SARS-CoV-2 virus in human plasma: Application to a bioequivalence study in Egyptian human volunteers인간 혈장에서 SARS-CoV-2 바이러스의 긴급 치료를 위한 잠재적인 항바이러스 후보 favipiravir의 결정을 위한 새로운 LC-MS/MS 방법: 이집트 인간 지원자의 생물학적 동등성 연구에 적용Article Published on 2021-05-302022-09-11 Journal: Journal of pharmaceutical and biomedical analysis [Category] SARS, 치료제, [키워드] acetic acetic acid adopted Antiviral antiviral agent application applied bioanalysis Bioequivalence Bioequivalence study composed Concentration COVID-19 Eclipse electrospray ionization Favipiravir flow rate healthy Human Human plasma isocratic LC–MS/MS Linear regression linearity m/z mass spectrometer methanol pharmacokinetic plasma sample positive protein precipitation pumped pyrazinamide quantification SARS-CoV-2 SARS-CoV-2 virus simple Treatment US-FDA Volunteer volunteers was used [DOI] 10.1016/j.jpba.2021.114057 PMC 바로가기 [Article Type] Article
Estimation of the Actual Incidence of Coronavirus Disease (COVID-19) in Emergent Hotspots: The Example of Hokkaido, Japan during February–March 2020Article Published on 2021-05-282022-10-30 Journal: Journal of Clinical Medicine [Category] COVID-19, [키워드] 95% confidence interval actual approach assessments confirmed case coronavirus disease COVID-19 COVID-19 case cumulative diagnosed Emerging infectious diseases Epidemiology estimates estimation incidence Infection initial Japan Linear regression model Mild no symptom outbreak pandemic predict provided public health quantification statistical framework Surveillance Transmission Travel medicine were used [DOI] 10.3390/jcm10112392 PMC 바로가기 [Article Type] Article
Point-of-care lung ultrasound in COVID-19 patients: inter- and intra-observer agreement in a prospective observational studyClinical Trial Published on 2021-05-212022-10-04 Journal: Scientific Reports [Category] SARS, 바이오마커, 임상, 치료기술, [키워드] analyzed consolidation coronavirus disease COVID-19 COVID-19 patients disease evaluated highest ICU intensive care investigator lowest lung median moderate pathology patients point-of-care prospective observational study quantification random recorded Respiratory distress syndrome Respiratory signs and symptoms score sequence single-center study subpleural Variability viral infection [DOI] 10.1038/s41598-021-90153-2 PMC 바로가기 [Article Type] Clinical Trial
SalivaSTAT: Direct-PCR and Pooling of Saliva Samples Collected in Healthcare and Community Setting for SARS-CoV-2 Mass SurveillanceArticle Published on 2021-05-192022-10-31 Journal: Diagnostics [Category] COVID-19, [키워드] alleviate CDC comparable COVID-19 diagnostic evaluated extraction extraction-free FDA homogenization limitation limitations mass media PerkinElmer pooling positive protocol quantification RT-PCR RT-PCR assay RT-PCR kits RT-PCR test Saliva saliva sample Sample SARS-CoV-2 setting stages tested Treatment widespread [DOI] 10.3390/diagnostics11050904 PMC 바로가기 [Article Type] Article
Assessing Biocompatibility of Face Mask Materials during COVID-19 Pandemic by a Rapid Multi-Assays StrategyArticle Published on 2021-05-182022-10-29 Journal: International Journal of Environmental Research an [Category] Coronavirus, SARS, [키워드] assays Assessing biocompatibility biologic collected coronavirus disease COVID-19 Cytokines cytotoxicity emergency situation evaluate face Face mask fibroblasts IL-6 in vivo incompatible indicated Inflammation interleukin-6 marker Mask material Materials MTT multi nitric oxide pandemic Predictive quantification Rapid skin fibroblast Strategy suggested tested variety [DOI] 10.3390/ijerph18105387 PMC 바로가기 [Article Type] Article
A real-time and high-throughput neutralization test based on SARS-CoV-2 pseudovirus containing monomeric infrared fluorescent protein as reporter적외선 형광단백단백을 리포터로 포함하는 SARS-CoV-2 유사바이러스 기반의 실시간 고처리량 중화시험Research Article Published on 2021-05-182022-09-10 Journal: Emerging Microbes & Infections [Category] SARS, 변종, 유전자 메커니즘, 진단, [키워드] animals antibody autofluorescence biosafety biosafety level Biosafety Level 3 biosafety level 3 laboratory Conventional correlated country D614G D614G mutation develop disease severity dual Efficacy flow cytometry fluorescent green fluorescent protein humans Infection Laboratory level 2 level 3 limit live SARS-CoV-2 luciferase mechanism monomeric monomeric infrared fluorescent protein natural infection neutralization neutralization assays neutralization test neutralization titer Neutralizing antibodies pandemic Particles plaque reduction neutralization Protein pseudovirus pseudoviruses quantification reporter S1 protein SARS-CoV-2 SARS-CoV-2 neutralization SARS-CoV-2 pseudovirus sensitivity sera shedding shown Support survival time-consuming vaccination Vaccine vaccine efficacy Variability variant [DOI] 10.1080/22221751.2021.1925163 PMC 바로가기 [Article Type] Research Article
Randomized clinical trial to evaluate safety and efficacy of convalescent plasma use among hospitalized patients with COVID-19 (PERUCONPLASMA): a structured summary of a study protocol for a randomized controlled trial입원 중인 COVID-19 환자의 회복기 혈장 사용의 안전성과 효능을 평가하기 위한 무작위 임상 시험(PERUCONPLASMA): 무작위 대조 시험을 위한 연구 프로토콜의 구조화된 요약Letter Published on 2021-05-172022-09-01 Journal: Trials [Category] SARS, 임상, 진단, 치료기술, [키워드] 1:1 ABO blood type Acute coronary syndrome administration Adverse reaction Adverse reactions aliquot Aliquots All participants allocation anaphylaxis antibody Antigen assessment Bilirubin Blinding block blocks blood gas blood gas analysis blood type C-reactive protein calculated Capacity carried case report form clinical clinical trial clinical trials Coagulation Coma completion confidence level congestive heart failure contraindication control group convalescent plasma coronary syndrome COVID-19 COVID-19 disease COVID-19 pneumonia creatinine Critical Critical disease CRP current D-dimer decrease defined detect Diagnosis dialysis disease Disseminated intravascular coagulation dissemination Donor donors drop Duration Dynamics eCRF Efficacy electronic case report form element eligible enrolled enrolment etiology evaluate evaluated events Evolution exclusion criteria expected experimental group exploratory failure following gas analysis hemodynamic instability hemorrhage hospital hospital discharge Hospitalization Hospitalized hospitalized patient hospitalized patients Hypothesis Hypoxemia ICU IIb in-hospital mortality Inclusion Infarction Inflammatory information Informed consent intensive care intensive care unit Intervention intracranial hemorrhage intravascular coagulation Invasive mechanical ventilation investigator investigators jaundice Laboratory laboratory confirmation Laboratory test Lactate lactate dehydrogenase Length less Local lymphocyte male marker Mechanical mechanical ventilation mmHg molecular Mortality multi-organ failure Myocardial infarction Neutralizing antibodies number objective occur Older open open label outcome oxygen Oxygen requirement oxygen saturation PaO participant Patient performed plasma Platelets Pneumonia power power of 80% previous diagnosis Probability protocol Public Hospital public hospitals quantification randomization Randomized Randomized controlled trial rationale receive reduce reducing reduction Registered resolution of symptom respiratory respiratory rate routine clinical practice Safe Safety Sample size SARS-COV-2 infection score Secondary objectives serious adverse reaction Serious adverse reactions serologic serum serum creatinine severe COVID-19 Severe COVID-19 pneumonia severe disease SOFA specific type standard treatment standard treatment alone statistical analysis Study protocol Stupor subject supplementary material Total total bilirubin transfusion treatment arm Trial uncontrolled vasopressors Ventilation website with COVID-19 [DOI] 10.1186/s13063-021-05189-6 PMC 바로가기 [Article Type] Letter
Using excess deaths and testing statistics to determine COVID-19 mortalitiesCovid-19 Published on 2021-05-172022-10-31 Journal: European Journal of Epidemiology [Category] COVID-19, SARS, [키워드] average complicate country COVID-19 COVID-19 mortality death deaths Denmark determine Disease prevalence Ecuador eight Epidemic excess deaths Factor fatality Germany IMPROVE in some Mortality mortality data New York City Norway Other quantification regions reported significantly higher Spain statistical statistically supplementary material Test statistics [DOI] 10.1007/s10654-021-00748-2 PMC 바로가기 [Article Type] Covid-19
Semen impairment and occurrence of SARS-CoV-2 virus in semen after recovery from COVID-19COVID-19 회복 후 정액 손상 및 정액 내 SARS-CoV-2 바이러스 발생Article Published on 2021-05-172022-09-11 Journal: Human reproduction (Oxford, England) [Category] SARS, 치료기술, [키워드] absence acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Administered affected age all subjects Analysis azoospermia biological fluid caution chance collected Contagion coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 COVID-19 severity cross-sectional cross-sectional study demonstrated ELISA enrolled evaluated Fertility form function General population healing Health Organization IL-8 impairment implication index Infection interleukin interleukin-8 leukocyte leukocytes limit men Nasopharyngeal swab Occurrence oligo-crypto-azoospermia parameter Participants Patient performed positive quantification Questionnaires registration number reproductive routine RT-PCR Saliva SARS-CoV-2 SARS-CoV-2 genome SARS-CoV-2 infections SARS-CoV-2 virus SARS-CoV2 Semen Semen analysis severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 sexual sexual transmission significantly specimen Statistical power tested the SARS-CoV-2 genome Urine virus virus RNA was performed World Health Organization [DOI] 10.1093/humrep/deab026 PMC 바로가기 [Article Type] Article
Dead space estimates may not be independently associated with 28-day mortality in COVID-19 ARDS데드 스페이스 추정치는 COVID-19 ARDS의 28일 사망률과 독립적으로 연관되지 않을 수 있습니다.Observational Study Published on 2021-05-172022-09-11 Journal: Critical Care [Category] 바이오마커, 임상, [키워드] 28-day mortality acute respiratory distress acute respiratory distress syndrome added Analysis ARDS baseline case sery Comorbidity consecutive patient consecutive patients Course COVID-19 dead Dead space dead space ventilation determine direct method eligible end-tidal estimate estimation fraction ICU impairment increase in increased risk information intensive care unit invasive ventilation marker mechanical ventilation Mortality multicenter National Netherlands non-survivor observational study outbreak Participation Patient prognostic Prognostic value Prognostication quantification Registered Respiratory dead space respiratory distress Respiratory distress syndrome Result retrospective risk secondary severity shown significantly significantly higher supplementary material surrogate markers survivor syndrome the dead Trial registration Ventilation ventilatory Ventilatory ratio [DOI] 10.1186/s13054-021-03570-0 PMC 바로가기 [Article Type] Observational Study