Identification of Natural Inhibitors Against SARS-CoV-2 Drugable Targets Using Molecular Docking, Molecular Dynamics Simulation, and MM-PBSA Approach분자 도킹, 분자 역학 시뮬레이션 및 MM-PBSA 접근 방식을 사용한 SARS-CoV-2 약물 표적에 대한 천연 억제제 식별Cellular and Infection Microbiology Published on 2021-08-122022-09-11 Journal: Frontiers in Cellular and Infection Microbiology [Category] MERS, SARS, 치료제, [키워드] (RdRp) 10-hydroxyaloin A active site Against Amino acid amino acid residue Analysis anti-SARS-CoV-2 bind binding site complex complexes Compound Cys145 docking score drug drugable targets Dynamics Efficacy experimental validation free energy Gibbs free energy hydrogen identification in silico in vitro in vivo inhibitor isoquercetin lead M pro MD simulation molecular molecular docking molecular dynamics MPro natural parameter physiochemical properties physiochemical property plant Principal component principal component analysis protease RdRP responsible Result RMSD RMSF RNA-dependent RNA polymerase SARS-CoV-2 SARS-CoV-2 spike screened Simulation spike stable complex target target protein the SARS-CoV-2 the Spike was performed [DOI] 10.3389/fcimb.2021.730288 PMC 바로가기 [Article Type] Cellular and Infection Microbiology
The Impact of Angiotensin-Converting Enzyme 2 ( ACE2 ) Expression Levels in Patients with Comorbidities on COVID-19 Severity: A Comprehensive ReviewReview Published on 2021-08-092022-10-29 Journal: Microorganisms [Category] COVID-19, [키워드] ACE2 acute respiratory syndrome age association bind binding cardiovascular diseases chronic respiratory diseases Comorbidities Comorbidity complex Comprehensive condition COPD coronavirus COVID-19 COVID-19 disease COVID-19 pandemic COVID-19 patients diabetes enzyme expression Factor Gender heart failure host cell receptor Impact increase in inhibitor Invasion Level lung Lung disease lung tissue male Myocardial obesity organ outcome pathophysiology patients patients with comorbidity Protein RAAS receptor Replication respiratory epithelium responsible review SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 spike severity of COVID-19 the SARS-CoV-2 tissues Viral receptor virus [DOI] 10.3390/microorganisms9081692 PMC 바로가기 [Article Type] Review
Highly variable SARS-CoV-2 spike antibody responses to two doses of COVID-19 RNA vaccination in patients with multiple myeloma다발성 골수종 환자에서 2회분의 COVID-19 RNA 백신 접종에 대한 고도로 가변적인 SARS-CoV-2 스파이크 항체 반응Comment Published on 2021-08-092022-09-12 Journal: Cancer cell [Category] MERS, SARS, 유전자 메커니즘, 진단, [키워드] Abstract Antibody Response COVID-19 dose hematology Multiple myeloma oncology Patient RNA SARS-CoV-2 SARS-CoV-2 spike vaccination Vaccine [DOI] 10.1016/j.ccell.2021.06.014 PMC 바로가기 [Article Type] Comment
SARS-CoV-2 infects human pancreatic β cells and elicits β cell impairmentSARS-CoV-2는 인간 췌장 β 세포를 감염시키고 β 세포 손상을 유발합니다Article Published on 2021-08-032022-09-11 Journal: Cell Metabolism [Category] SARS, 바이오마커, [키워드] ACE2 Apoptosis apoptotic attenuate beta cell Cell Cell signaling cells coronavirus disease Coronavirus disease 2019 COVID-19 COVID-19 severity demonstrated diabete diabetes elicit Evidence expressed expression Factor impairment in vitro indicate induce infect insulin islet killing neuropilin neuropilin 1 NRP1 pancreatic pancreatic beta cell pandemic Pathway analysis Patient Phosphoproteomics receptor rescued SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 receptor SARS-CoV-2 spike SARS-CoV-2 spike protein secretion severe COVID-19 Signaling Spike protein T1D tested the SARS-CoV-2 TMPRSS2 Type 1 diabetes while [DOI] 10.1016/j.cmet.2021.05.013 PMC 바로가기 [Article Type] Article
A Single-Cycle Influenza A Virus-Based SARS-CoV-2 Vaccine Elicits Potent Immune Responses in a Mouse ModelArticle Published on 2021-08-032022-10-28 Journal: Vaccines [Category] COVID-19, [키워드] 5′ UTR Antigen antigenic C-terminus Cell Cell-mediated immunity chimeric cytoplasmic demonstrated domain elicited exhibited expressed expressing Frame gene encoding hosts humoral immune response immunized increasingly infected cell Influenza Influenza virus inoculated MDCK cell Model mouse mucosal Neutralizing activity ORF passages peptide-based platform potent prime-boost regimen producing Protein Pseudotyped virus RBD response robust SARS-CoV-2 SARS-CoV-2 spike SARS-CoV-2 vaccine sera Seven single-cycle influenza virus-based vaccine spike RBD spike-pseudotyped virus the RBD the SARS-CoV-2 the vaccine Vaccine Vaccine development variants virus [DOI] 10.3390/vaccines9080850 PMC 바로가기 [Article Type] Article
Dynamics of SARS-CoV-2 Spike Proteins in Cell Entry: Control Elements in the Amino-Terminal Domains세포 진입에서 SARS-CoV-2 스파이크 단백질의 역학: 아미노 말단 도메인의 제어 요소Research Article Published on 2021-08-032022-09-10 Journal: mBio [Category] MERS, SARS, 변종, [키워드] ACE2 receptors acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 adaptive amplify associations binding cell entry change changes in comparison Comparisons complex Control coronavirus coronavirus spike protein coronavirus spike proteins correlated domain driven by Dynamics effective element Extracellular greater inactivation Increased INDEL indels Infection initial membrane fusion murine NTD NTDs Quantitative RBD RBDs Receptor binding receptor-binding domains reduced Region respiratory sarbecovirus SARS-CoV-2 SARS-CoV-2 spike SARS-CoV-2 spike protein SARS-CoV-2 variant SARS-CoV-2 variants severe acute respiratory syndrome Coronavirus spike Spike protein Spike proteins spikes stability Structure Substitution substitutions susceptible the receptor-binding domain the SARS-CoV-2 Trimers variant Variation variations virus virus entry virus infectivity virus receptors Virus-like particle virus-like particles [DOI] 10.1128/mBio.01590-21 PMC 바로가기 [Article Type] Research Article
Potent neutralizing nanobodies resist convergent circulating variants of SARS-CoV-2 by targeting diverse and conserved epitopesArticle Published on 2021-08-032022-10-04 Journal: Nature Communications [Category] MERS, SARS, 변종, [키워드] ACE2-binding site acute respiratory syndrome Analysis antibodies Antiviral bind binding binding epitope circulating variant circulating variants class comparison conserved coronavirus COVID-19 therapeutics cryo-EM structures Cryoelectron microscopy determine disrupt effective eight epitope host receptor inaccessible indicate mechanisms Microbiology Microscopy nanobody neutralization neutralize Neutralizing Neutralizing activity neutralizing antibody neutralizing epitope potent provided RBD Receptor binding domain recognize reveal SARS-CoV SARS-CoV-2 SARS-CoV-2 spike Spike protein STABLE structural biology Structure target the SARS-CoV-2 unique Vaccine variant variants of concern variety virus VoC VOCs [DOI] 10.1038/s41467-021-24963-3 PMC 바로가기 [Article Type] Article
Characterization of a Lineage C.36 SARS-CoV-2 Isolate with Reduced Susceptibility to Neutralization Circulating in Lombardy, Italy계통의 특성화 C.36 SARS-COV-2 분리 물의 중화에 대한 감수성 감소가 이탈리아 롬바르디아에서 순환Brief Report Published on 2021-07-312022-09-01 Journal: Viruses [Category] SARS, 변종, 진단, [키워드] characterization concerning COVID-19 deletions evade genomic sequencing humoral Humoral response isolate Isolation Italy L452R Lineage lombardy molecular neutralization Phenotypes R346K recurrence SARS-CoV-2 SARS-CoV-2 isolate SARS-CoV-2 isolates SARS-CoV-2 spike SARS-CoV-2 strain sera single-point mutation single-point mutations spike susceptibility traits Transmissibility Vaccine variant of concern [DOI] 10.3390/v13081514 PMC 바로가기 [Article Type] Brief Report
The SARS-CoV-2 spike L452R-E484Q variant in the Indian B.1.617 strain showed significant reduction in the neutralization activity of immune seraResearch Article Published on 2021-07-302022-10-28 Journal: Precision Clinical Medicine [Category] COVID-19, [키워드] addition amino acid substitution B.1.617 B.1.617.1 binding biologic comparable Convalescent patients COVID-19 D614G dominant variant E484Q ELISA assay Evolution of SARS-CoV-2 Factor Health authority healthy highlight immune implication India Infectivity involved L452R mRNA vaccine neutralization neutralization activity neutralizing antibody non-human primate non-human primates Non-synonymous outbreak Protein Pseudotyped virus RBD Receptor binding domain reduction in SARS-CoV-2 SARS-CoV-2 spike SARS-CoV-2 variant sera the antibody the RBD the spike protein vaccination Vaccine development vaccinee variant virus [DOI] 10.1093/pcmedi/pbab016 PMC 바로가기 [Article Type] Research Article
Rapid and stable mobilization of CD8 + T cells by SARS-CoV-2 mRNA vaccineSARS-CoV-2 mRNA 백신에 의한 CD8 + T 세포의 빠르고 안정적인 동원Article Published on 2021-07-282022-09-10 Journal: Nature [Category] Fulltext, SARS, 진단, 치료기술, [키워드] affected Analysis Arm association BNT162b2 boost CD4 CD8 circulating detectable distribution early stage effector cells epitope exhibited functional functional capacity Immunological memory longitudinal Longitudinal analyses Lymphocyte differentiation maturation mediators memory memory T memory T cell memory T cells mRNA mRNA vaccine mRNA vaccines natural infection Neutralizing antibodies neutralizing antibody precursor Rapid RNA vaccines robust SARS-CoV-2 SARS-CoV-2 spike severe disease subset T cell T cell response T cell responses T cells vaccination Vaccine-induced immunity viral infection [DOI] 10.1038/s41586-021-03841-4 PMC 바로가기 [Article Type] Article