The YXXΦ motif within the severe acute respiratory syndrome coronavirus (SARS-CoV) 3a protein is crucial for its intracellular transportResearch Published on 2014-04-242022-10-28 Journal: Virology Journal [Category] Coronavirus, [키워드] absence accumulated acute respiratory syndrome Apoptosis Aprotinin association Brefeldin A carried Cell coronavirus Degradation detect distribution expressed function Golgi immunofluorescence Increased induce lipid droplets lysosomal marker motif mRNA mutant Mutation plasma membrane Protein protein level protein synthesis Proteins residue Result reversed RT-PCR SARS Coronavirus SARS-CoV significantly lower staining subcellular distribution Transcription transfected with translation Transport transported tyrosine viral pathogenesis was performed while wild type [DOI] 10.1186/1743-422X-11-75 PMC 바로가기 [Article Type] Research
Approaching the Functional Annotation of Fungal Virulence Factors Using Cross-Species Genetic Interaction Profiling종간 유전적 상호작용 프로파일링을 사용하여 진균 독성 인자의 기능적 주석 접근Research Article Published on 2012-12-272022-09-06 Journal: PLoS Genetics [Category] Communicable Disease, [키워드] accurate AIDS analyzed annotation approach Author C. neoforman C. neoformans C. neoformans gene C. neoformans genes calculation Cancer Cell cell surface Chemotherapy compared conserved correlated correlation correlations Cryptococcus neoforman Cryptococcus neoformans death deletion mutant deletion mutants demonstrated develop disease enhancement expressed expression Factor found function functional functional annotation fungal fungal pathogens fungi Gene gene deletion gene function Genetic genetic interaction genetic interactions Golgi Golgi apparatus growth HIV/AIDS HIV/AIDS patients human pathogen identify immunostimulatory individuals Infection inhibiting Interaction interactions lack mannose molecular mutant Organ transplant organism Organisms ortholog pathogen pathogenicity pathogenicity protein Pathogens patients phagocytosis predict predicted profile profiles Profiling promote quantification Quantitative recipient required S. cerevisiae Saccharomyces cerevisiae sequence supported suppression susceptible tested the cell understanding utility validation virulence virulence gene yeast [DOI] 10.1371/journal.pgen.1003168 PMC 바로가기 [Article Type] Research Article
A Single Polar Residue and Distinct Membrane Topologies Impact the Function of the Infectious Bronchitis Coronavirus E ProteinResearch Article Published on 2012-05-032022-10-29 Journal: PLoS Pathogens [Category] Coronavirus, [키워드] activities adopted affected Antiviral strategies Author coronavirus correlated defined different disrupt disrupting domain E protein examined found function functions Golgi Host hydrophobic IBV identify Impact implicated in vitro independent infected cells Infection infectious Infectious Bronchitis Virus Infectious virus less mapped mechanism of action membrane membrane protein mutant occur Particle Particles predicted promote Protein reported required residue secretory pathway Substitution thought Topology transmembrane virion assembly virus [DOI] 10.1371/journal.ppat.1002674 PMC 바로가기 [Article Type] Research Article
Human Coronavirus NL63 Open Reading Frame 3 encodes a virion-incorporated N-glycosylated membrane proteinResearch Published on 2010-01-152022-10-28 Journal: Virology Journal [Category] Coronavirus, [키워드] accessory protein addition Alpha Amino acid Analysis antiserum C-terminus Cell conserved coronavirus Coronaviruses cytosolic detected digestion ENCODE ERGIC Evidence expression Extracellular FLAG-tagged fluorescence microscopy Frame function Golgi Huh-7 cell Human human coronavirus immunofluorescence In-vitro Infected lysosomal membrane membrane protein N-terminus NL63 nucleocapsid Occurrence ORF pathogenic coronavirus predicted Protein purified reading Respiratory tract infections Result sequence shown structural protein subsequent Topology translation viral particle virion [DOI] 10.1186/1743-422X-7-6 PMC 바로가기 [Article Type] Research