In Silico Molecular Dynamics of Griseofulvin and Its Derivatives Revealed Potential Therapeutic Applications for COVID-19Griseofulvin 및 그 파생물의 In Silico 분자 역학, COVID-19에 대한 잠재적인 치료 응용 프로그램 공개Article Published on 2022-06-212022-09-11 Journal: International Journal of Molecular Sciences [Category] COVID19(2023년), SARS, 신약개발, 치료제, [키워드] ACE2 Analysis angiotensin angiotensin-converting enzyme 2 anti-SARS-CoV-2 application approved drug approved drugs Computational approaches coronavirus disease Coronavirus disease 2019 COVID-19 derivative docking docking score domain Drug repurposing Dynamics Efficacy examined followed by future therapeutic griseofulvin griseofulvin derivatives highest human host hydrogen hydrogen bonds inhibitor inhibitory molecular molecular docking analysis molecular dynamics option Potential protease RBD RdRP Revealed SARS-CoV-2 SARS-COV-2 infection Spike protein Therapeutic approach therapeutic approaches [DOI] 10.3390/ijms23136889 PMC 바로가기 [Article Type] Article
Production of a functionally active recombinant SARS-CoV-2 (COVID-19) 3C-like protease and a soluble inactive 3C-like protease-RBD chimeric in a prokaryotic expression system원핵생물 발현 시스템에서 기능적으로 활성인 재조합 SARS-CoV-2(COVID-19) 3C 유사 프로테아제 및 가용성 불활성 3C 유사 프로테아제-RBD 키메라의 생산Article Published on 2022-06-202022-09-11 Journal: Epidemiology and Infection [Category] COVID19(2023년), SARS, 치료제, [키워드] 3C-like protease acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 addition antigenicity approach blocked carrier cells Chimer chimeric chymotrypsin co-expressed complex coronavirus COVID-19 COVID-19 vaccines cysteine cysteine protease cysteine proteases dimeric disease drug ELISAs enzyme Escherichia coli Escherichia coli BL21 exhibit expression function inactive inhibitors inhibitory main protease non-structural protein non-structural proteins Papain Papain-like protease PLPro polyproteins pp1a pp1ab processing produced Production prokaryotic expression system protease Proteases Protein purification RBD Receptor binding domain RTC SARS-CoV-2 Serine serum severe acute respiratory syndrome Coronavirus solubility Spike protein target tested the Spike Transcription vaccinated individual vaccinated individuals viral replication [DOI] 10.1017/S0950268822001078 PMC 바로가기 [Article Type] Article
Synergistic anti-SARS-CoV-2 activity of repurposed anti-parasitic drug combinations용도 변경 구충제 조합의 상승적 항 SARS-CoV-2 활성Article Published on 2022-06-182022-09-11 Journal: BMC Pharmacology & Toxicology [Category] COVID19(2023년), SARS, 신약개발, 치료제, [키워드] Analysis anti-parasitic drug Anti-parasitic drugs Anti-SARS-CoV-2 Activity antiviral drug antiviral drugs approach benefit Calu Calu-3 calu-3 cells Chloroquine claimed clinical trials Combination combinations country COVID-19 pandemic demonstrated drug drug combination drug combinations drugs Economy effective Effectiveness ENhance epithelial cells evaluated Health Service highest in vitro inhibitory inhibitory concentration Ivermectin Lung epithelial cells niclosamide pandemic reduction in Repurposed drug repurposing resulting SARS-CoV-2 shown Spread synergistic synergy tested Treatment vaccination Vero E6 Vero E6 cell VERO E6 cells [DOI] 10.1186/s40360-022-00580-8 PMC 바로가기 [Article Type] Article
Human Superantibodies to 3CL pro Inhibit Replication of SARS-CoV-2 across Variants3CL pro에 대한 인간 초항체는 변이체 전반에 걸쳐 SARS-CoV-2의 복제를 억제합니다Article Published on 2022-06-132022-09-11 Journal: International Journal of Molecular Sciences [Category] COVID19(2023년), SARS, 변종, [키워드] 3CL pro 3CLpro Betacoronavirus betacoronaviruses binding blocked catalytic activity Cell cell-penetrating antibody clone conserved Contact E. coli effective enzyme Escherichia coli Host Human human cells human single-chain antibody variable fragments (HuscFvs) in vitro infect inhibited inhibitory investigated Major major protease (3CLpro) membrane omicron Omicron variants peptide phage predicted produced protease Replication residue residues Safe SARS-CoV-2 single-chain antibody superantibody. tested the SARS-CoV-2 variant virus replication was used were used wild type Wuhan [DOI] 10.3390/ijms23126587 PMC 바로가기 [Article Type] Article
Early administration of COVID-19 convalescent plasma with high titer antibody content by live viral neutralization assay is associated with modest clinical efficacy살아있는 바이러스 중화 분석에 의한 고역가 항체 함량을 가진 COVID-19 회복기 혈장의 조기 투여는 보통의 임상 효능과 관련이 있습니다Article Published on 2022-06-012022-09-11 Journal: American journal of hematology [Category] COVID19(2023년), MERS, SARS, 변종, 진단, [키워드] absolute reduction administration administration of CCP antibody Antibody titer benefit CCP clinical benefit Clinical efficacy convalescent plasma correlation COVID-19 COVID-19 convalescent plasma Cox regression death rate death rates determined by develop Dilution Efficacy evaluate evaluated Hospitalization hospitalized patient hospitalized patients inhibitory intubated patients multivariable Cox regression neutralized Neutralizing antibody titer Non-intubated non-intubated patient non-intubated patients Patient receiving Risk factors shown stratified titer transfused Treatment two groups Viral viral neutralization viral variant Viral variants was performed with COVID-19 [DOI] 10.1002/ajh.26531 PMC 바로가기 [Article Type] Article
Ursodeoxycholic acid ameliorates cell migration retarded by the SARS-CoV-2 spike protein in BEAS-2B human bronchial epithelial cellsUrsodeoxycholic acid는 BEAS-2B 인간 기관지 상피 세포에서 SARS-CoV-2 스파이크 단백질에 의해 지연된 세포 이동을 개선합니다Article Published on 2022-06-012022-09-11 Journal: Biomedicine & pharmacotherapy = Biomédecine & phar [Category] COVID19(2023년), SARS, 유전자 메커니즘, [키워드] ACE2 ACE2 expression airway analyzed angiotensin angiotensin converting enzyme angiotensin converting enzyme 2 basal layer BEAS-2B Beas-2B bronchial epithelial cell BEAS-2B cell Beas-2B cells binding bronchial epithelial cell caused Cell Cell migration cell proliferation cells changes in conditions contribute control cells coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 damage damaged differentiation DNA ENhance enzyme epithelial epithelial cell flow cytometry followed by Gene Expression greater HEK293T Hela immunostaining inhibitory Interaction investigated mechanism Migration overexpression Plasmid Prevent Protective RBD receptor Receptor-binding domain reduced repair repair mechanism Repair mechanisms restoration restored RT-PCR SARS- CoV-2 SARS-CoV-2 spike SARS-CoV-2 spike protein significantly Single Cell SP binding Spike protein syndrome the receptor-binding domain the SARS-CoV-2 the spike protein transfection Treatment Ursodeoxycholic acid Ursodeoxycholic acid (UDCA). virus infection was increased was measured western blotting wound healing [DOI] 10.1016/j.biopha.2022.113021 PMC 바로가기 [Article Type] Article
Inhibition of SARS-CoV-2 replication by zinc gluconate in combination with hinokitiol히노키티올과 함께 글루콘산아연에 의한 SARS-CoV-2 복제 억제Article Published on 2022-06-012022-09-11 Journal: Journal of inorganic biochemistry [Category] COVID19(2023년), SARS, 치료제, [키워드] 3CLpro acute respiratory syndrome coronavirus antiviral therapy approach binding catalytic site chymotrypsin Combination Concentration coronavirus 2 coronavirus disease Coronavirus disease 2019 Coronavirus infections COVID-19 elevated Enzyme inhibition exhibited experimental evidence global public health gluconate hinokitiol in vitro inhibit inhibited inhibition inhibitory Ionophore mammalian cells metal ion MPro pandemic Papain Papain-like protease PLPro potential benefit potential benefits protease Protease activity Proteases protein structure proteolytic proteolytic activity residues respiratory SARS-CoV-2 SARS-CoV-2 replication screened Severe acute respiratory syndrome severe acute respiratory syndrome coronavirus 2 significantly small molecule target Treatment Zinc zinc gluconate Zinc gluconate. [DOI] 10.1016/j.jinorgbio.2022.111777 PMC 바로가기 [Article Type] Article
Single-cell transcriptome profiling of the immune space-time landscape reveals dendritic cell regulatory program in polymicrobial sepsis면역 시공간 환경의 단일 세포 전사체 프로파일링은 다균성 패혈증에서 수지상 세포 조절 프로그램을 보여줍니다Article Published on 2022-05-292022-09-11 Journal: Theranostics [Category] 치료제, [키워드] activated adopted alteration Analysis applied available data Cancer canonical CD4 cDCs characterized co-cultured comprehensive view conserved COVID-19 patients Cytometry demonstrated dendritic dendritic cell dendritic cells described determined by dissect experiment experiments expression Flow flow cytometry generate Genes heterogeneity Host immune response hyperinflammation identify IFNGR2 immune Immune cell immune cell types immune cells immunofluorescence Immunosuppression indicated inhibitory initiated Intracellular majority maturation morbidity multiplex immunofluorescence myeloid cells naïve natural killer observation organ dysfunction pathway Pathways progression promoted protein level Puncture rationale Regulatory regulatory T cells reveal scRNA-seq ScRNA-seq analysis Sepsis septic septic patients signaling pathway single-cell single-cell analysis Single-cell RNA sequencing specific type staining STAT3 subset T cell T cells tissue tissue sites Transcriptome unique upregulation [DOI] 10.7150/thno.72760 PMC 바로가기 [Article Type] Article
Repurposing an In Vitro Measles Virus Dissemination Assay for Screening of Antiviral Compounds항바이러스 화합물의 스크리닝을 위한 시험관내 홍역 바이러스 보급 분석의 용도 변경Review Published on 2022-05-292022-09-11 Journal: Viruses [Category] 치료제, [키워드] Adults Antiviral antiviral assay Antiviral assays antiviral efficacy approved can not children Compound contagious contribute Coverage COVID-19 pandemic described dissemination effective evaluate fusion inhibitory peptide immunocompromised individuals immunoglobulins in vitro individual Infant inhibit inhibitory Intervention intravenous lifted limit Measle measles measles virus morbidity and mortality outbreak Outbreaks pandemic pandemic response box peptide populations present reactive Remdesivir repurposing respiratory virus responsible resulting resurgence risk Safe Screening Standard of care therapeutic interventions Treatment vaccination Vaccine [DOI] 10.3390/v14061186 PMC 바로가기 [Article Type] Review
Impact of an Immune Modulator Mycobacterium-w on Adaptive Natural Killer Cells and Protection Against COVID-19면역 조절제 Mycobacterium-w가 적응성 자연 살해 세포 및 COVID-19에 대한 보호에 미치는 영향Article Published on 2022-05-042022-09-12 Journal: Frontiers in Immunology [Category] COVID19(2023년), MERS, SARS, 진단, [키워드] adaptive adaptive NK cells ADCC administration Against antibody-dependent cellular cytotoxicity baseline both groups Cell Cohort control group correlated COVID-19 downregulation expression healthcare worker high-risk population IFN-γ Impact incidence inhibitory Innate immunity modulator Mw for COVID-19 Mycobacterium w (Mw) Mycobacterium natural natural killer NKG2A NKG2C non-immune pathway Pathways protection provided reduction in risk RNA-seq analysis SARS-CoV-2 SARS-COV-2 infection severity of COVID-19 translated upregulation Virus Disease [DOI] 10.3389/fimmu.2022.887230 PMC 바로가기 [Article Type] Article