Engineering of Chinese Hamster Ovary Cells With NDPK-A to Enhance DNA Nuclear Delivery Combined With EBNA1 Plasmid Maintenance Gives Improved Exogenous Transient Reporter, mAb and SARS-CoV-2 Spike Protein ExpressionBioengineering and Biotechnology Published on 2021-06-042022-10-31 Journal: Frontiers in Bioengineering and Biotechnology [Category] COVID-19, [키워드] approach cell line characterisation Chinese Chinese hamster ovary cells CHO cells co-expression Combined complementary complex delivery dependent on Dilution DNA Efficiency element Engineering ENhance Epstein Barr Nuclear Antigen-1 Exogenous expressing Gene Expression glycoprotein growth hamster Improved initial mAb maintenance mammalian cell membranes NLS nuclear nuclear localisation signal nucleoside diphosphate kinase nucleus Ovary plasma Plasmid Protein reduce SARS-CoV-2 SARS-CoV-2 spike SARS-CoV-2 spike protein spike time-consuming Transient transient expression transient gene expression utilised [DOI] 10.3389/fbioe.2021.679448 PMC 바로가기 [Article Type] Bioengineering and Biotechnology
DNA vaccination induced protective immunity against SARS CoV-2 infection in hamsterssResearch Article Published on 2021-05-272022-10-28 Journal: PLoS Neglected Tropical Diseases [Category] COVID-19, SARS, [키워드] ACE2 acute respiratory syndrome analyses analyzed antibody antigen-specific antibody approach binding blocked cloned collected coronavirus COVID-19 COVID-19 epidemic COVID-19 pandemic COVID-19 vaccine demonstrated DNA DNA vaccine Efficacy Electroporation elicited entry receptor expression expression vectors global public health hamster hamsters Health HEK293T cells humoral immune response immunization in vivo inactivated induce Infection levels of antibody long-lasting mammalian cell neutralizing antibody Neutralizing antibody titer outbreak pandemic Protective protective effect protective immunity Protein respond SARS CoV-2 SARS-CoV SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 RBD SARS-CoV-2 S SARS-CoV-2 vaccine sera serum antibody Side effects spike gene Spike protein Spike protein expression suggested T cell response Th1 Th1-biased immune response the receptor-binding domain the SARS-CoV-2 transient expression vaccination Vaccine virus [DOI] 10.1371/journal.pntd.0009374 PMC 바로가기 [Article Type] Research Article
Purification and characterization of the receptor‐binding domain of SARS‐CoV‐2 spike protein from Escherichia coliResearch Article Published on 2021-05-072022-10-31 Journal: Engineering in Life Sciences [Category] COVID-19, [키워드] ACE2 antigenic epitope binding characterized COVID‐19 COVID‐19 drug E. coli Escherichia coli expected expressed Flow glycosylation HEK293 cell Host host cell inhibitors lack low expression mammalian cell Neutralizing antibodies pandemic Protein protein expression purification purified RBD receptor‐binding domain responsible SARS‐CoV‐2 SARS‐CoV‐2 secondary structure severe respiratory disease spike Spike protein the vaccine Vaccines viral entry virus [DOI] 10.1002/elsc.202000106 PMC 바로가기 [Article Type] Research Article
Vaccines based on replication incompetent Ad26 viral vectors: Standardized template with key considerations for a risk/benefit assessmentReview article Published on 2021-05-012022-10-05 Journal: Vaccine [Category] SARS, 비임상, [키워드] Ad26 adenoviral vector Adenovirus baseline Benefit/Risk cellular immune responses Characteristics clinical clinical evaluation clinical study clinical trials collaboration COVID-19 vaccine database Ebola virus effective evaluate evaluated Express expression Extensive feature FIVE flexible Genetic group Human humoral key consideration mammalian cell Neutralizing antibody titer New participant Phase 3 platform pre-existing immunity profiles regimen Replication Replication-incompetent Ad26 risk Safety T cell response transgene Trial Vaccine Vaccine development vaccine immunogenicity Vaccines variety vector vectors Viral vector Viral vector vaccines working [DOI] 10.1016/j.vaccine.2020.09.018 [Article Type] Review article
Single-Cell RNA-seq Reveals Angiotensin-Converting Enzyme 2 and Transmembrane Serine Protease 2 Expression in TROP2 + Liver Progenitor Cells: Implications in Coronavirus Disease 2019-Associated Liver DysfunctionMedicine Published on 2021-04-222022-10-31 Journal: Frontiers in Medicine [Category] COVID-19, [키워드] ACE2 acute respiratory syndrome analyzed angiotensin-converting enzyme 2 binding caused cell death cell population China cirrhotic liver co-expression Comorbidities coronavirus coronavirus disease COVID-19 COVID-19 patient Cytokine storms Deleterious develop disease fetal FIVE globe healthy host cell human liver impair implicated indicated liver Liver cirrhosis liver dysfunction liver tissue mammalian mammalian cell pandemic pathophysiology Patient prevalent progenitor Protein receptor reported respiratory symptom Respiratory virus infection RNA-Seq SARS-CoV-2 scRNA-seq serine protease significantly Single Cell single-cell RNA-seq spike the S protein tissue TMPRSS2 transmembrane serine protease Trop2 viral infection [DOI] 10.3389/fmed.2021.603374 PMC 바로가기 [Article Type] Medicine
Potential APOBEC-mediated RNA editing of the genomes of SARS-CoV-2 and other coronaviruses and its impact on their longer term evolutionArticle Published on 2021-04-012022-10-28 Journal: Virology [Category] COVID-19, MERS, SARS, [키워드] Amino acid antiviral activity APOBEC base changes in coronavirus coronavirus genomes Coronaviruses COVID-19 pandemic Cytidine deaminase cytosine determine directional DNA viruses Evidence Evolution Genome genomic hallmark herpesvirus homoplasy Innate immunity knowledge mammalian cell member Occurrence other coronavirus other coronaviruses Potential reported RNA RNA virus SARS-CoV-2 SARS-CoV-2 genome sequence SARS-CoV-2 sequence sequence sustained variant Viral proteins Virus evolution [DOI] 10.1016/j.virol.2020.12.018 PMC 바로가기 [Article Type] Article
Bepridil is potent against SARS-CoV-2 in vitroBepridil은 시험관 내 SARS-CoV-2에 강력합니다.410 Published on 2021-03-092022-08-13 Journal: Proceedings of the National Academy of Sciences of [Category] Biochemistry, COVID-19, SARS, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Analysis animal model animal models Antiviral bepridil Cell characterized Clinical tests Clinical use Concentration coronavirus COVID-19 COVID-19 patients docking dose-dependent manner drug Drug administration dual ebastine effective endosomal pH food function Host human cell in vitro indicated infected cells inhibit inhibiting interfere Live virus main protease mammalian cell medication Medicine microneutralization pathogen pimozide protease raising respiratory SARS-CoV-2 SARS-CoV-2 entry severe acute respiratory syndrome Coronavirus Significance small molecule Small molecules tested Vero E6 [DOI] 10.1073/pnas.2012201118 PMC 바로가기 [Article Type] 410
Development of Spike Receptor-Binding Domain Nanoparticles as a Vaccine Candidate against SARS-CoV-2 Infection in FerretsResearch Article Published on 2021-03-022022-10-29 Journal: mBio [Category] COVID-19, SARS, [키워드] ACE2 acute respiratory syndrome angiotensin-converting enzyme 2 Antigen Body weight loss candidate clearance Clinical symptoms coronavirus CoV COVID-19 demonstrated development disease domain effective ferret ferritin Fever Helicobacter pylori Host host cell immunization induce Infectious virus Interaction intramuscular intranasal lung mammalian cell nanoparticle nasal washe neutralizing antibody organs pandemic produced protective immunity Protein purified RBD RBD-nanoparticle Receptor-binding domain SARS-CoV-2 SARS-COV-2 infection spike the RBD the spike protein Vaccine vaccine candidate Vaccines Viral RNA [DOI] 10.1128/mBio.00230-21 PMC 바로가기 [Article Type] Research Article
Atlas of ACE2 gene expression reveals novel insights into transmission of SARS-CoV-2Research Article Published on 2020-12-262022-10-31 Journal: Heliyon [Category] COVID-19, [키워드] 2019-nCoV ACE2 ACE2 expression ACE2 gene analyzed Atla carrier caused clinical symptom conserved COVID-19 COVID-19 patients DNA expresse expression expression pattern Host hosts human tissue infect mammal mammalian mammalian cell mammals MOST novel Novel coronavirus observé pandemic peptide Potential Potential host reveal SARS-CoV-2 susceptibility tissue transmembrane protein transmission of SARS-CoV-2 viral infection yielding [DOI] 10.1016/j.heliyon.2020.e05850 PMC 바로가기 [Article Type] Research Article
An ultrapotent synthetic nanobody neutralizes SARS-CoV-2 by stabilizing inactive SpikeReport Published on 2020-12-182022-10-28 Journal: Science (New York, N.y.) [Category] COVID-19, [키워드] ACE2 acute respiratory syndrome affinity airway epithelia angiotensin-converting enzyme 2 Anti-spike antibody Bacteria bind binding cells coronavirus cryo-EM disrupt group host cell host cell receptor inaccessible inactive Interaction intravenously mammalian cell maturation Microscopy multivalent nanobodies nanobody neutralization neutralize potent produced Protein Receptor binding domain SARS-CoV-2 SARS-COV-2 infection Science screened selected spike stability synthetic nanobody the spike protein therapeutic Treatment trivalent virus yeast [DOI] 10.1126/science.abe3255 PMC 바로가기 [Article Type] Report