Two pan-SARS-CoV-2 nanobodies and their multivalent derivatives effectively prevent Omicron infections in miceArticle Published on 2023-01-122024-09-05 Journal: Cell Reports Medicine [Category] update2024, [키워드] cross-reactive antibody IgM antibody inhalable administration nanobody omicron sarbecovirus SARS-CoV-2 [DOI] 10.1016/j.xcrm.2023.100918 PMC 바로가기 [Article Type] Article
Screening, Expression, and Identification of Nanobody against SARS-CoV-2 Spike ProteinArticle Published on 2022-10-242023-07-10 Journal: Cells [Category] COVID19(2023년), [키워드] nanobody phage display SARS-CoV-2 single-domain antibody Spike protein [DOI] 10.3390/cells11213355 PMC 바로가기
A computational study of metal-organic frameworks (MOFs) as potential nanostructures to combat SARS-CoV-2Article Published on 2022-09-202022-11-15 Journal: Scientific Reports [Category] SARS, 치료제, [키워드] ACE acute respiratory syndrome angiotensin-converting enzyme 2 antibody antiviral drug binding biological molecule carrier coronavirus COVID-19 Critical deformation determine drug host cells inhibitor Interaction investigated M pro MOF molecular nanobody non-structural protein protease Protein receptor S protein SARS-CoV-2 secondary structure Spike protein surface protein tested Vaccine virus replication [DOI] 10.1038/s41598-022-19845-7 PMC 바로가기
Isolation of an escape-resistant SARS-CoV-2 neutralizing nanobody from a novel synthetic nanobody libraryArticle Published on 2022-09-162022-11-15 Journal: Frontiers in Immunology [Category] SARS, 변종, 진단, [키워드] accelerated affinity Affinity maturation amplification antibodies antibody binding CDR Cdr1 CDR2 COVID-19 COVID-19 pandemic Electroporation expand fusion protein humanized IC50 Isolation moderate Mutagenesis Mutation nanobody neutralization Neutralizing omicron performed plaque reduction neutralization PRNT randomization RBD Reactions Receptor binding domain robust SARS-CoV-2 SARS-CoV-2 neutralization SARS-CoV-2 variant SARS-CoV-2 variants subsequent synthetic library. therapeutic potential trimeric Vaccine development variant VHH [DOI] 10.3389/fimmu.2022.965446 PMC 바로가기
Mucosal nanobody IgA as inhalable and affordable prophylactic and therapeutic treatment against SARS-CoV-2 and emerging variantsArticle Published on 2022-09-122022-11-15 Journal: Frontiers in Immunology [Category] SARS, 변종, 진단, [키워드] administration antibodies Antibody therapeutics Antiviral antiviral prophylaxis and therapeutics Biological sciences comparable conserved effective epitope fusion protein IgA IgG Fc Immunity infected with SARS-CoV-2 Infection intranasal invasive K18-ACE2 less Lineage mammalian Microbiology mucosal nanobody neutralization neutralize omicron Pichia pastoris produced Prophylactic Prophylaxis provide RBD recognize SARS-CoV-2 SARS-CoV-2 spike protein SARS-CoV-2 variant severe respiratory disease Therapeutic treatment transgenic mice variant variants VoC [DOI] 10.3389/fimmu.2022.995412 PMC 바로가기
Arsenal of nanobodies shows broad-spectrum neutralization against SARS-CoV-2 variants of concern in vitro and in vivo in hamster modelsArticle Published on 2022-09-092022-11-15 Journal: Communications Biology [Category] SARS, 변종, 진단, 치료기술, [키워드] anti-RBD anti-S2 anti-SARS-CoV-2 antibody fragment arsenal candidate characterized conserved cross-reactivity demonstrated detectable Efficacy epitope feasibility IgG in vitro in vivo intrinsic mAb magnitude nanobodies nanobody neutralization offer provide reducing SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 variant SARS-CoV-2 variants shown specificity spike glycoprotein therapeutic thermodynamic viral burden [DOI] 10.1038/s42003-022-03866-z PMC 바로가기
A humanized nanobody phage display library yields potent binders of SARS CoV-2 spike인간화된 나노바디 파지 디스플레이 라이브러리는 SARS CoV-2 스파이크의 강력한 결합제를 생성합니다Article Published on 2022-08-102022-09-11 Journal: PLoS ONE [Category] SARS, 변종, 진단, [키워드] (alpha affinity antibody antibody treatment B.1.1.7 binding modes complex effective Ex vivo humanized mitigate molecular dynamics Molecular dynamics simulation molecular dynamics simulations N501Y N501Y mutation nanobodies nanobody neutralization Neutralizing Neutralizing antibodies primary human airway RBD RBD mutation reducing SARS CoV-2 SARS-CoV-2 SARS-CoV-2 infections SARS-CoV-2 spike protein SARS-CoV-2 variants shown Spike protein Structural analysis the SARS-CoV-2 variant Viral viral burden WA1 [DOI] 10.1371/journal.pone.0272364 PMC 바로가기 [Article Type] Article
Hetero-bivalent nanobodies provide broad-spectrum protection against SARS-CoV-2 variants of concern including OmicronArticle Published on 2022-07-292022-10-05 Journal: Cell Research [Category] COVID19(2023년), SARS, 변종, 유전자 메커니즘, 진단, 치료제, [키워드] adaptive antibody benefit Beta binding binding affinity broad-spectrum antiviral caused complex Contact COVID-19 crystal structure Delta ENhance epitope fused fusing Gamma hamster Infection Lambda mice mouse-adapted SARS-CoV-2 Mutation nanobody neutralization activity neutralized omicron Prophylactic prophylactically provide provided Pseudotyped viruses RBD RBD residue resolved SARS-CoV-2 SARS-CoV-2 variant tested the RBD the receptor-binding domain the spike protein therapeutic Treatment variant variants variants of concern wild-type Wuhan [DOI] 10.1038/s41422-022-00700-3 [Article Type] Article
A Novel Nanobody-Horseradish Peroxidase Fusion Based-Competitive ELISA to Rapidly Detect Avian Corona-Virus-Infectious Bronchitis Virus Antibody in Chicken SerumArticle Published on 2022-07-082023-08-30 Journal: International Journal of Molecular Sciences [Category] E형 간염, [키워드] avian coronavirus competitive ELISA Infectious Bronchitis Virus nanobody phage display technology. [DOI] 10.3390/ijms23147589 PMC 바로가기
A panel of nanobodies recognizing conserved hidden clefts of all SARS-CoV-2 spike variants including OmicronOmicron을 포함한 모든 SARS-CoV-2 스파이크 변이체의 보존된 숨겨진 틈을 인식하는 나노바디 패널Article Published on 2022-07-062022-09-11 Journal: Communications Biology [Category] COVID19(2023년), MERS, SARS, 변종, 진단, 치료기술, 치료제, [키워드] Accessibility Analysis antibodies antibody caused clone conserved Contact coronavirus COVID-19 detect diagnostics ELISA epitope Epitopes FIVE flow cytometric hidden Human Imbalance Infectious disease kinetic lateral flow Medicines Microscopy N-terminal domain nanobodies nanobody neutralize NTD omicron pandemic populations pseudotyped RBD Receptor-binding domain recognizing regions SARS-CoV-2 SARS-CoV-2 spike protein SARS-CoV-2 spike proteins SARS-CoV-2 spike variant SARS-CoV-2 variant Spike proteins spike variants target the receptor-binding domain the spike protein Vaccines Viral viral infection VOCs western blotting [DOI] 10.1038/s42003-022-03630-3 PMC 바로가기 [Article Type] Article