The Production of Standardized Samples with Known Concentrations for Severe Acute Respiratory Syndrome Coronavirus 2 RT-qPCR Testing Validation for Developing Countries in the Period of the Pandemic EraResearch Article Published on 2021-08-032022-10-27 Journal: BioMed Research International [Category] COVID-19, MERS, SARS, [키워드] Accuracy acute respiratory syndrome Bioanalytical Method Validation Biosafety Level 3 caused Concentration coronavirus coronavirus 2 coronavirus disease country COVID-19 criterion database dependent on Developing diagnose diagnostic evaluated Factors gold standard implementation inactivated Laboratory less limit limit of detection Linear regression NCBI pandemic pandemic era Period Pneumonia Precision Production provide public health Quantitative R 2 reached respiratory Result reverse transcription PCR RT-qPCR Sample SARS-CoV-2 SARS-CoV-2 testing Sensitivity and specificity Spread synthesized RNA Testing the disease threat validation virus sample [DOI] 10.1155/2021/5516344 PMC 바로가기 [Article Type] Research Article
The Production of Standardized Samples with Known Concentrations for Severe Acute Respiratory Syndrome Coronavirus 2 RT-qPCR Testing Validation for Developing Countries in the Period of the Pandemic Era중증 급성 호흡기 증후군 코로나 바이러스 2 RT-QPCR 테스트 검증을위한 공지 된 농도를 갖는 표준화 된 샘플의 생산은 전염병 시대의 개발 도상국을위한 국가를위한 국가에 대한 검증Research Article Published on 2021-08-032022-09-10 Journal: BioMed Research International [Category] 진단, 치료기술, [키워드] Accuracy acute respiratory syndrome acute respiratory syndrome coronavirus Bioanalytical Method Validation biosafety level Biosafety Level 3 biosafety level 3 laboratory caused Concentration coronavirus coronavirus 2 coronavirus disease Coronavirus disease 2019 country COVID-19 criterion database dependent on Developing diagnose diagnostic diagnostic testing evaluated Factors gold gold standard implementation inactivated Laboratory less level 3 limit limit of detection Linear regression linearity long term NCBI pandemic pandemic era Period Pneumonia Precision Production provide public health Quantitative R 2 reached respiratory Result reverse transcription PCR RT-qPCR Sample SARS-CoV-2 SARS-CoV-2 testing Sensitivity and specificity severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 Spread synthesized RNA Testing the disease threat validation verification virus sample [DOI] 10.1155/2021/5516344 PMC 바로가기 [Article Type] Research Article
Comparison of Immunological Profiles of SARS-CoV-2 Variants in the COVID-19 Pandemic Trends: An Immunoinformatics ApproachArticle Published on 2021-05-062022-10-27 Journal: Antibiotics [Category] COVID-19, [키워드] antigenicity approach B-cell epitope circulating variant Combination combinations concerning COVID-19 COVID-19 cases COVID-19 pandemic COVID-19 severity descendant determine disease disease severity docking Efficacy England epidemiological epidemiological fitness epitope evaluated FIVE GISAID greater Hospital admission Human IEDB immune responses immunogenic profile immunoinformatics immunoinformatics approach immunological parameter increased risk India infection rate less moderate Mortality mutant Mutation NCBI Neutralizing neutralizing capacity non-allergic non-toxic pathogen Probability profile Protein protein sequence recorded reported SARS-CoV-2 SARS-CoV-2 antigenicity SARS-CoV-2 epitope prediction SARS-CoV-2 immunogenicity SARS-CoV-2 variant SARS-CoV-2 variants selected epitope sequence severe disease severity severity of COVID-19 simulation studies South Africa spike gene Spike protein T-cell the SARS-CoV-2 virus the spike protein the vaccine Toxicity translated Transmission USA vaccine modification variant variants Viremia virus Wuhan strain [DOI] 10.3390/antibiotics10050535 PMC 바로가기 [Article Type] Article
Data mining of coronavirus: SARS-CoV-2, SARS-CoV and MERS-CoVResearch Note Published on 2021-04-202022-10-27 Journal: BMC Research Notes [Category] COVID-19, [키워드] Amino acid Apriori BLAST Characteristics codon coronavirus decision Decision tree experiment experiments Incubation period initial Machine learning algorithms MERS-CoV NCBI objective outbreak question Result SARS-CoV SARS-CoV-2 SARS-CoV-2 virus sequence shown similarity SVM transmitted tree virus [DOI] 10.1186/s13104-021-05561-4 PMC 바로가기 [Article Type] Research Note
Data mining of coronavirus: SARS-CoV-2, SARS-CoV and MERS-CoVResearch Note Published on 2021-04-202022-10-27 Journal: BMC Research Notes [Category] Coronavirus, COVID-19, SARS, [키워드] Amino acid Apriori BLAST Characteristics codon coronavirus decision Decision tree experiment experiments Incubation period initial Machine learning algorithms MERS-CoV NCBI objective outbreak question Result SARS-CoV SARS-CoV-2 SARS-CoV-2 virus sequence shown similarity SVM transmitted tree virus [DOI] 10.1186/s13104-021-05561-4 PMC 바로가기 [Article Type] Research Note
Deepening of In Silico Evaluation of SARS-CoV-2 Detection RT-qPCR Assays in the Context of New Variants새로운 변이체의 맥락에서 SARS-CoV-2 검출 RT-qPCR 분석의 실리코 평가 심화Article Published on 2021-04-132022-09-10 Journal: Genes [Category] MERS, SARS, 변종, 진단, 치료기술, 치료제, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 annealing bioinformatics tool China CDC collected Context coronavirus coronavirus disease COVID-19 COVID-19 diagnosis decrease Deepening detection evaluate evaluated Evolution Genome GISAID highlight impede in silico in silico Approach in silico specificity evaluation independent investigated Mismatches Mutation NCBI New new SARS-CoV-2 oligonucleotide oligonucleotides pandemic performed primer Primers primers and probes probe probe sequence probe sequences probes qPCR qPCR assays reported respiratory RT-qPCR RT-qPCR assay RT-qPCR assays SARS-CoV-2 SARS-CoV-2 detection SARS-CoV-2 genome SARS-CoV-2 genomes SARS-CoV-2 testing SARS-CoV-2 variants severe acute respiratory syndrome Coronavirus unique variants variants of concern Viral Viral RNA virus WGS data [DOI] 10.3390/genes12040565 PMC 바로가기 [Article Type] Article
The phylogenetic relationship within SARS-CoV-2s: An expanding basal cladeSARS-CoV-2s 내의 계통발생적 관계: 확장되는 기초 분기군Article Published on 2021-04-012022-09-11 Journal: Molecular phylogenetics and evolution [Category] SARS, 변종, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 added Analysis basal Basal clade caused clade collected coronavirus COVID-19 COVID-19 pandemic cross dataset exhibit Genome genome replication GISAID high mutation rate human host identify mutated Mutation mutation rate NCBI nonstructural protein nsp13 outbreak Pairwise comparison Parsimony principle Phylogenetic relationship Positive selection Proofreading RNA RNA proofreading capability SARS-CoV-2 SARS-CoV-2 genome sequences SARS-CoV-2 sequence SARS-CoV-2 sequences sequence Sequence alignment Seven severe acute respiratory syndrome Coronavirus species the SARS-CoV-2 Transmission unique viral sequence viral sequences whole genome sequence [DOI] 10.1016/j.ympev.2020.107017 PMC 바로가기 [Article Type] Article
Whole-genome sequencing of SARS-CoV-2 reveals the detection of G614 variant in PakistanSARS-CoV-2의 전체 게놈 시퀀싱은 파키스탄에서 G614 변이체의 검출을 보여줍니다Research Article Published on 2021-03-232022-08-13 Journal: PLoS ONE [Category] SARS, 유전자 메커니즘, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Amino acid Analysis aspartic acid characteristic China clade coronavirus D614G D614G mutation Diego Evolution FIVE G614 variant genetic diversity Genome Genome sequences genomic GISAID glycine highlight Illumina Inc. India lead library Missense Mutation mutations NCBI Oman oropharyngeal swab oropharyngeal swabs pandemic Patient Phylogenetic analysis positive PrEP prevalent real-time RT-PCR Region respiratory reveal RNA SARS-CoV-2 SARS-CoV-2 strain selected sequence sequences Sequencing severe acute respiratory syndrome Coronavirus shown Slovakia Spike protein Spread strain Strains submitted United States virus Virus evolution viruses was performed whole genome Whole genome sequencing whole-genome whole-genome sequencing [DOI] 10.1371/journal.pone.0248371 PMC 바로가기 [Article Type] Research Article
Genomic Variations in SARS-CoV-2 Genomes From Gujarat: Underlying Role of Variants in Disease Epidemiology구자라트의 SARS-CoV-2 게놈의 게놈 변이: 질병 역학에서 변이의 기본 역할Genetics Published on 2021-03-192022-08-13 Journal: Frontiers in Genetics [Category] 유전자 메커니즘, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 affected allele frequency analyzed average caused clade Cluster coronavirus coronavirus disease Coronavirus disease 2019 country Course COVID-19 dataset datasets death death rate deaths Deceased Deleterious Ebola Evolution genomic Genomic surveillance GISAID globe Gujarat Haplotype haplotyping highest in viral include India list measles MERS Middle East Middle East respiratory syndrome Missense mutation Missense mutations Mortality Mutation mutation analysis National NCBI Novel coronavirus nucleocapsid ORF3a P -value Pandemics past Pathogenesis Pathogens Patient phylogenetic distribution recovered patient recovered patients respiratory role SARS-CoV-2 SARS-CoV-2 (2019-nCoV) SARS-CoV-2 genome SARS-CoV-2 genomes sequenced Severe acute respiratory syndrome severe acute respiratory syndrome Coronavirus significantly syndrome variant variants Viral viral epidemiology viral genome viral genomes viral pathogenesis virulence while [DOI] 10.3389/fgene.2021.586569 PMC 바로가기 [Article Type] Genetics
Phylo-geo-network and haplogroup analysis of 611 novel coronavirus (SARS-CoV-2) genomes from India인도의 611개 신종 코로나바이러스(SARS-CoV-2) 게놈의 계통-지리 네트워크 및 하플로그룹 분석Research Article Published on 2021-03-162022-09-10 Journal: Life Science Alliance [Category] SARS, 변종, 유전자 메커니즘, [키워드] A2a accession number Analysis analyzed appear China clade country Epidemic full-length genome Genome GISAID initiative had more haplogroup India Lineage maximum NC_045512.2 NCBI network Novel coronavirus observation observations pandemic pangolin predominant prevalent lineage reference sequence remaining Repository SARS-CoV-2 SARS-CoV-2 genome SARS-CoV-2 genomes sequence Viral viral evolution Wuhan [DOI] 10.26508/lsa.202000925 PMC 바로가기 [Article Type] Research Article