Microfluidic Chip with Two-Stage Isothermal Amplification Method for Highly Sensitive Parallel Detection of SARS-CoV-2 and Measles VirusArticle Published on 2021-12-192022-10-30 Journal: Micromachines [Category] COVID-19, [키워드] acute respiratory syndrome amplification clinical specificity clinically coronavirus cross-contamination detection Detection limit Diagnostic method identify isothermal amplification lowest Measle measles virus Microfluidic chip molecular multiplex Nasopharyngeal swab nucleic acid offer parallel molecular diagnostic POCT point-of-care polymerase real time RPA SARS-CoV-2 sensitive sensitivity Sensitivity and specificity target tested two-stage isothermal amplification virus [DOI] 10.3390/mi12121582 PMC 바로가기 [Article Type] Article
A chemical-enhanced system for CRISPR-Based nucleic acid detectionCRISPR 기반 핵산 검출을 위한 화학 강화 시스템Article Published on 2021-11-152022-09-11 Journal: Biosensors & bioelectronics [Category] SARS, 치료기술, 치료제, [키워드] addition albumin amplification bovine bovine serum albumin buffer Chemical condition COVID-19 COVID-19 pandemic CRISPR detection Detection sensitivity determine determined by develop diagnostics effectors ENhance enhanced help Inclusion isothermal amplification L-proline LAMP lateral flow strip nucleic acid Nucleic acid detection parameter Pathogens point-of-care provide RPA SARS-CoV-2 SARS-CoV-2 pseudovirus secure serum albumin shown viral pathogens [DOI] 10.1016/j.bios.2021.113493 PMC 바로가기 [Article Type] Article
Using Cy5-dUTP labelling of RPA-amplicons with downstream microarray analysis for the detection of antibiotic resistance genesArticle Published on 2021-10-112022-10-04 Journal: Scientific Reports [Category] 진단, [키워드] amplicons amplification antibiotic resistance gene Biological techniques Cell detect diagnostics Disadvantages downstream dTTP Escherichia coli flexible Genes Genomic DNA isothermal isothermal amplification method less microarray analysis Molecular biology overcome pathogen performed point of care primer Primers probe provide RPA sensitivity Target genes [DOI] 10.1038/s41598-021-99774-z PMC 바로가기 [Article Type] Article
An ultrafast SARS-CoV-2 virus enrichment and extraction method compatible with multiple modalities for RNA detectionRNA 검출을 위한 다중 양식과 호환되는 초고속 SARS-CoV-2 바이러스 농축 및 추출 방법Randomized Controlled Trial Published on 2021-10-022022-09-11 Journal: Analytica chimica acta [Category] MERS, SARS, 임상, 진단, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus affinity Affinity nanoparticles alternative method alternative methods amplification Analysis Chain Reaction characterization characterized clinical samples clinically comparable compatibility Concordance coronavirus Coronavirus-2 COVID-19 pandemic demonstrated diagnoses Dilution dilutions double-blind study Enzymatic extraction form isothermal amplification Laboratory LAMP Loop-mediated amplification (LAMP) Loop-mediated isothermal amplification miniaturization nasopharyngeal swabs Particle pathogenicity polymerase polymerase chain polymerase chain reaction Polymerase chain reaction (PCR) positive recombinase polymerase amplification Recombinase polymerase amplification (RPA) respiratory reverse transcription reverse transcription real-time polymerase chain reaction RNA RNA eluate RNA isolation RNA virus RPA RT-PCR Saliva SARS-CoV-2 SARS-CoV-2 viral SARS-CoV-2 virus Severe acute respiratory syndrome severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 severe acute respiratory syndrome coronavirus-2 Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) solution Stage the stage transmission rate Viral viral titer viral titers viral transport media VTM zoonotic [DOI] 10.1016/j.aca.2021.338846 PMC 바로가기 [Article Type] Randomized Controlled Trial
Isothermal recombinase polymerase amplification-lateral flow detection of SARS-CoV-2, the etiological agent of COVID-19Article Published on 2021-10-012023-06-19 Journal: Journal of virological methods [Category] MERS, SARS, [키워드] coronavirus diagnostics lateral flow point-of-care RPA SARS-CoV-2 [DOI] 10.1016/j.jviromet.2021.114227 PMC 바로가기
The Effects of Physical Inactivity and Exercise at Home in Young Patients with Congenital Heart Disease during the COVID-19 PandemicArticle Published on 2021-09-252022-10-29 Journal: International Journal of Environmental Research an [Category] COVID-19, SARS, [키워드] BMI carried CHD concerning congenital congenital heart disease COVID-19 COVID-19 mitigation measure COVID-19 pandemic decrease disease Effect effective Effects enrolled exercise Exercise training heart home hospital less lockdown material pandemic parameter Patient performed physical activity physically questionnaire risk RPA significant increase significantly Stress subject Tolerance [DOI] 10.3390/ijerph181910065 PMC 바로가기 [Article Type] Article
Sensitive electrochemical biosensor combined with isothermal amplification for point-of-care COVID-19 tests현장 진료 COVID-19 테스트를 위한 등온 증폭과 결합된 민감한 전기화학 바이오센서Article Published on 2021-06-152022-09-11 Journal: Biosensors & bioelectronics [Category] SARS, 치료기술, 치료제, [키워드] accumulate acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Amplicon amplicons amplification Combination comparable coronavirus COVID-19 COVID-19 test current density diagnosis of COVID-19 Electrochemical detection method equipment gel electrophoresis hybridization isothermal amplification limits Limits of detection N gene PCR platform point-of-care polymerase primer pulse purification RdRP RdRp gene REcombinase recombinase polymerase amplification reduction RPA SARS-CoV-2 sensitive severe acute respiratory syndrome Coronavirus target gene thiol [DOI] 10.1016/j.bios.2021.113168 PMC 바로가기 [Article Type] Article
A Ligation/Recombinase Polymerase Amplification Assay for Rapid Detection of SARS-CoV−2SARS-CoV−2의 신속한 검출을 위한 결찰/재조합효소 중합효소 증폭 분석Cellular and Infection Microbiology Published on 2021-05-282022-09-11 Journal: Frontiers in Cellular and Infection Microbiology [Category] MERS, SARS, 치료기술, 치료제, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus-2 addition addressed amplification caused Chain Reaction China CDC clinical samples comparable Concentration Coronavirus-2 deaths detection Diagnosis DNA equipment less Ligase ligation Mutation nucleic acid Nucleic acid detection optimization ORF1 ORF1ab pandemic pandemic of COVID-19 polymerase polymerase chain polymerase chain reaction Primers principle probe quantitative polymerase chain reaction Rapid reaction recombinase polymerase amplification reported respiratory reverse transcription RNA templates RPA RT-qPCR SARS-CoV-2 sensitivity Severe acute respiratory syndrome severe acute respiratory syndrome Coronavirus specific biomarker specific biomarkers T4 DNA ligase Transcription Viral Viral RNA virus would increase [DOI] 10.3389/fcimb.2021.680728 PMC 바로가기 [Article Type] Cellular and Infection Microbiology
Combined recombinase polymerase amplification/rkDNA-graphene oxide probing system for detection of SARS-CoV-2SARS-CoV-2 검출을 위한 결합된 재조합 효소 중합효소 증폭/rkDNA-그래핀 옥사이드 프로빙 시스템Article Published on 2021-05-082022-09-11 Journal: Analytica chimica acta [Category] SARS, 치료기술, 치료제, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 added amplification appear Combined complementary coronavirus Diagnosis DNA amplification Enzymatic in situ synthetic probe (rkDNA) exhibited Fluorescence Graphene Graphene oxide help Infection isothermal isothermal amplification N-gene pandemic virus point-of-care polymerase Prevent probe RPA SARS-CoV-2 selective selectivity sensitivity sequence severe acute respiratory syndrome Coronavirus Spread target [DOI] 10.1016/j.aca.2021.338390 PMC 바로가기 [Article Type] Article
Development of a reverse transcription recombinase polymerase amplification assay for rapid and direct visual detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)중증급성호흡기증후군 코로나바이러스 2(SARS-CoV-2)의 신속하고 직접적인 시각적 검출을 위한 역전사 재조합효소 중합효소 증폭 분석법 개발Research Article Published on 2021-01-062022-08-13 Journal: PLoS ONE [Category] 유전자 메커니즘, 치료기술, [키워드] acute respiratory syndrome coronavirus Amplification assay analytical sensitivity carried clinical clinical sensitivity coronavirus coronavirus 2 costly Cross reactivity Detection limit development diagnosing Diagnosis genomic RNA Intervention isothermal amplification lateral flow Nasopharyngeal samples new virus outbreak PCR polymerase polymerase chain polymerase chain reaction positive Rapid real time Real time reverse transcription polymerase chain reaction recombinase polymerase amplification respiratory reverse transcription RNA RPA RT-qPCR RT-qPCR assay RT-qPCR assays RT-RPA SARS-CoV-2 Sensitivity and specificity severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 specificity SYBR Green SYBR Green I tested viruses [DOI] 10.1371/journal.pone.0245164 PMC 바로가기 [Article Type] Research Article