Cell surface SARS-CoV-2 nucleocapsid protein modulates innate and adaptive immunity세포 표면 SARS-CoV-2 뉴클레오캡시드 단백질은 선천성 및 후천성 면역을 조절합니다Article Published on 2022-08-052022-09-11 Journal: Science Advances [Category] SARS, 변종, 치료기술, 치료법, 치료제, [키워드] activate adaptive antibody antigenicity bind Cell cell surface cells chemokine chemokines chemotaxis Combination conserved CoVs cross-reactive cytosol detect electrostatic heparan sulfate heparin high affinity high-affinity binding human CoVs immune cells Immunity induce infected cells inhibited Innate immune cells Innate immunity leukocyte leukocytes live cells MERS-CoV modulate neighboring cell neighboring cells nucleocapsid protein Protein released SARS-CoV-1 SARS-CoV-2 SARS-CoV-2 nucleocapsid SARS-CoV-2 variant SARS-CoV-2 variants SARS-CoV-2-infected cell SARS-CoV-2-infected cells sequestering Strains sulfate sulfated T cell T cell responses transfected cell Vaccine zoonotic [DOI] 10.1126/sciadv.abp9770 PMC 바로가기 [Article Type] Article
Assessing and improving the validity of COVID-19 autopsy studies – A multicentre approach to establish essential standards for immunohistochemical and ultrastructural analysesResearch article Published on 2022-08-022022-10-05 Journal: EBioMedicine [Category] Coronavirus, SARS, 치료기술, [키워드] Alliance Analysis antibody approach Assessing Autopsy autopsy study available data Berlin cell line cellular structure center correlated COVID-19 criteria education Effect Effects electron microscopy evaluated finding foundation funding Guidance help high variability highest IHC immune response immunohistochemistry Interpretation Microscopy Ministry of Health multicentre organ organ damage pathology pathophysiology positive correlation Protein provided Quantitative recommendation reproducibility RT-qPCR SARS-CoV-2 SARS-CoV-2 detection SARS-CoV-2 nucleocapsid SARS-CoV-2 viral RNA Sensitivity and specificity specificity the SARS-CoV-2 tissue tissues university Validity Variability Viral protein virus [DOI] 10.1016/j.ebiom.2022.104193 [Article Type] Research article
Low molecular weight chitooligosaccharide inhibits infection of SARS-CoV-2 in vitro저분자량 키토올리고당은 시험관 내에서 SARS-CoV-2의 감염을 억제합니다Article Published on 2022-08-012022-09-11 Journal: Journal of Applied Microbiology [Category] COVID19(2023년), SARS, 치료법, 치료제, [키워드] Analysis Antiviral Antiviral effect antiviral effects candidate chitooligosaccharide chitosan COVID-19 cytotoxicity dose-dependent manner Drug development E gene fluorescence intensity gel in vitro infected cells Infection inhibit low molecular weight molecular weight MTT natural antiviral natural treatment. permeation Plaque assay Protein Quantitative RdRP respond SARS-CoV-2 SARS-CoV-2 nucleocapsid SARS-CoV-2 virus Significance suggested tested the SARS-CoV-2 virus Treatment Vero cells Viral RNA virus-infected cell virus-infected cells was measured [DOI] 10.1111/jam.15618 PMC 바로가기 [Article Type] Article
CRISPR-Cas12a-mediated label-free electrochemical aptamer-based sensor for SARS-CoV-2 antigen detectionSARS-CoV-2 항원 검출을 위한 CRISPR-Cas12a 매개 무표지 전기화학 앱타머 기반 센서Article Published on 2022-08-012022-09-11 Journal: Bioelectrochemistry (Amsterdam, Netherlands) [Category] SARS, 치료기술, 치료법, [키워드] amplification Antigen aptamer Biomarkers cleavage competitive binding complex COVID-19 CRISPR CRISPR-Cas12a cross-reactivity diagnostic DNA electrochemical biosensor expand generate gold impedance in vitro nucleocapsid antigen Quantitative resulting Rolling circle amplification SARS-CoV-2 SARS-CoV-2 antigen SARS-CoV-2 antigens SARS-CoV-2 nucleocapsid selective significant decrease significantly [DOI] 10.1016/j.bioelechem.2022.108105 PMC 바로가기 [Article Type] Article
Safety and immunogenicity of intramuscular, single-dose V590 (rVSV-SARS-CoV-2 Vaccine) in healthy adults: Results from a phase 1 randomised, double-blind, placebo-controlled, dose-ranging trial건강한 성인을 대상으로 한 단일 용량 V590(rVSV-SARS-CoV-2 Vaccine)의 근육 내 안전성 및 면역원성: 1상 무작위 배정, 이중 맹검, 위약 대조, 용량 범위 시험 결과Clinical Trial Published on 2022-08-012022-09-11 Journal: EBioMedicine [Category] COVID19(2023년), MERS, SARS, 임상, 진단, 치료법, [키워드] Administered administration Adults adverse event adverse events AEs anti-SARS-CoV-2 Antibody responses comparable COVID-19 COVID-19 vaccine Day dose double-blind Endpoint fatigue global pandemic headache healthy highest dose immunogenicity Inc. Injection-site pain intramuscular lower dose neutralisation neutralising antibody response Older overcome Part participant pfu Phase 1 phase 1 study Placebo placebo-controlled plaque plaque-forming unit plaque-forming units primary endpoints PRNT50 randomised receive reduction Registration reported Result Safety SARS-CoV-2 SARS-CoV-2 nucleocapsid seronegative seronegative participant seronegative participants seropositive serum Sharp spike-specific antibody response stomatitis subsidiary Trial USA V590 Vaccine vesicular stomatitis vesicular stomatitis virus vesicular stomatitis virus (VSV) virus VSV [DOI] 10.1016/j.ebiom.2022.104138 PMC 바로가기 [Article Type] Clinical Trial
Utility of Newborn Dried Blood Spots to Ascertain Seroprevalence of SARS-CoV-2 Antibodies Among Individuals Giving Birth in New York State, November 2019 to November 20212019년 11월부터 2021년 11월까지 뉴욕주에서 출생한 개인들 사이에서 SARS-CoV-2 항체의 혈청유병성을 확인하기 위한 신생아 건조 혈액 반점의 유용성Article Published on 2022-08-012022-09-11 Journal: JAMA Network Open [Category] SARS, 진단, 치료기술, [키워드] 95% CI age analyzed association Birth Birth weight Blood can be used Characteristics collected conducted correlated COVID-19 COVID-19 case COVID-19 vaccination cross-sectional cross-sectional study cumulative DBS sample detect Dried blood spots exposure to female fluctuation gestational age Giving identify IgG antibodies IgG antibody immunoassay individual individuals Infant Infants Low birth weight Lower male maternal age median microsphere MONITOR Multiple birth New York Newborn Odds ratio Older outcome pregnant Prevalence Program reflected repeated reported S antigen SARS-CoV-2 SARS-CoV-2 antibodies SARS-CoV-2 antibody SARS-CoV-2 N SARS-CoV-2 nucleocapsid SARS-CoV-2 seroprevalence Screening screening program Seroprevalence Serosurvey Serosurveys significantly lower spot state stratified study period submitted utility vaccination Vaccinations was measured women [DOI] 10.1001/jamanetworkopen.2022.27995 PMC 바로가기 [Article Type] Article
SARS-CoV-2 nucleocapsid protein triggers hyperinflammation via protein-protein interaction-mediated intracellular Cl – accumulation in respiratory epitheliumSARS-CoV-2 뉴클레오캡시드 단백질은 단백질-단백질 상호작용 매개 세포내 Cl-호흡 상피에서의 축적을 통해 과염증을 유발합니다Article Published on 2022-07-272022-09-11 Journal: Signal Transduction and Targeted Therapy [Category] COVID19(2023년), SARS, 신약개발, 치료법, [키워드] abrogated Airway inflammation CAMP cell lines cells CFTR Concentration COVID-19 cystic fibrosis Cytokine storms Defense demonstrated depleting downregulated elicit epithelial epithelial cell epithelial cells expression fibrosis glucocorticoid Host host defense human airway epithelial hyperinflammation immune response immune responses Inflammatory response Inflammatory responses inhibition inhibitor Intracellular knockout microRNA modulating murine model murine models N protein pathogen phosphodiesterase Phosphorylation promoted Protein pulmonary inflammation raised reducing regulated respiratory epithelium resulting robust Rolipram SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 nucleocapsid second messenger selective serum SGK1 signaling pathway Smad3 structural protein suggested sustained targeting therapeutic strategy transmembrane Trigger upregulation [DOI] 10.1038/s41392-022-01048-1 PMC 바로가기 [Article Type] Article
The global succinylation of SARS-CoV-2-infected host cells reveals drug targetsSARS-CoV-2에 감염된 숙주 세포의 세계적인 숙시닐화는 약물 표적을 드러낸다Article Published on 2022-07-262022-09-11 Journal: Proceedings of the National Academy of Sciences of [Category] Coronavirus, COVID19(2023년), SARS, 변종, 치료법, 치료제, [키워드] Analysis Antiviral antiviral drug antiviral drug target antiviral effects antiviral therapies Caco-2 Caco-2 cells causative agent cellular cellular pathway cellular pathways conserved COVID-19 COVID-19 pandemic demonstrated drug target enzyme Evolution highlighting Host host cell host protein inhibit inhibitor Interaction mechanism membrane protein Membrane proteins metabolic pathways Modification nsp14 overexpressed promote Protein Quantitative regulated Regulatory reveal SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 nucleocapsid SIRT5 sirtuin succinylproteomics. variants Viral proteins virus replication [DOI] 10.1073/pnas.2123065119 PMC 바로가기 [Article Type] Article
Smartphone-Based High-Throughput Fiber-Integrated Immunosensing System for Point-of-Care Testing of the SARS-CoV-2 Nucleocapsid ProteinSARS-CoV-2 뉴클레오캡시드 단백질의 현장 진료 테스트를 위한 스마트폰 기반 고처리량 섬유 통합 면역 감지 시스템Article Published on 2022-07-222022-09-11 Journal: ACS Sensors [Category] COVID19(2023년), SARS, 치료기술, 치료법, [키워드] 95% CI acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Analysis blood sample Blood samples clinical centers clinically Community confidence interval coronavirus coronavirus disease Coronavirus disease 2019 correlated COVID-19 COVID-19 patient cut-off value diagnosed diagnosis of COVID-19 ELISA ELISA kit healthy blood donor healthy blood donors high-throughput detection hospital identify Infection limit of detection linear membrane negative control sample nucleocapsid protein optical Optical fiber pandemic Protein SARS-CoV-2 SARS-CoV-2 nucleocapsid SARS-CoV-2 nucleocapsid protein Sensitivity and specificity serum sample serum samples severe acute respiratory syndrome Coronavirus significantly simple Smartphone System tested Testing the SARS-CoV-2 track-etched membrane. [DOI] 10.1021/acssensors.2c00754 PMC 바로가기 [Article Type] Article
Kinetics of anti-nucleocapsid IgG response in COVID-19 immunocompetent convalescent patientsCOVID-19 면역적격 회복기 환자에서 항뉴클레오캡시드 IgG 반응의 동역학Article Published on 2022-07-202022-09-11 Journal: Scientific Reports [Category] COVID19(2023년), MERS, SARS, 진단, 치료기술, 치료법, [키워드] absence anti-Nucleocapsid IgG anti-SARS-CoV-2 anti-SARS-CoV-2 humoral antibody Antibody Response Antibody testing BMI comprehension convalescent patient Convalescent patients country COVID-19 COVID-19 convalescent patient COVID-19 disease decrease detectable develop disease severity humoral humoral immune response IgG IgG antibodies IgG antibody immunodeficiency inclusion criteria Kinetics knowledge longitudinal study majority Nasopharyngeal swab nasopharyngeal swab sample Nasopharyngeal swab samples nucleocapsid Patient positive Protein Re-infection robust RT- PCR SARS-CoV-2 SARS-CoV-2 nucleocapsid serum Sex Treatment Vaccination strategies Vaccination strategy virus infection [DOI] 10.1038/s41598-022-16402-0 PMC 바로가기 [Article Type] Article