A high-resolution temporal atlas of the SARS-CoV-2 translatome and transcriptomeSARS-CoV-2 트랜스라톰 및 트랜스크립톰의 고해상도 시간 지도Article Published on 2021-08-252022-09-10 Journal: Nature Communications [Category] MERS, SARS, 변종, 유전자 메커니즘, 진단, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 caused coronavirus COVID-19 deaths downstream enhancer evaluate function genomic help High-resolution human cells Human genome identify infecting initiate leader sequence mRNA ORF ORFs provide regulated Regulation respiratory resulting SARS-CoV-2 Sequencing severe acute respiratory syndrome Coronavirus sRNA subgenomic RNA subgenomic RNAs suppressor the SARS-CoV-2 Transcriptome translation translation initiation unique [DOI] 10.1038/s41467-021-25361-5 PMC 바로가기 [Article Type] Article
Comprehensive mapping of SARS-CoV-2 interactions in vivo reveals functional virus-host interactions생체 내 SARS-CoV-2 상호 작용의 포괄적 인 매핑은 기능적 바이러스-숙주 상호 작용을 나타냅니다Article Published on 2021-08-252022-09-10 Journal: Nature Communications [Category] MERS, SARS, 변종, 유전자 메커니즘, 진단, [키워드] approach bind Cell cells cellular Comprehensive element epigenomics functional Genome global health Health Host host mRNA identify Illumina in viral in vivo Interaction interactions Methylation mitochondrial molecular mutant observé pathogenicity platform regions report reveal RNA RNA sequencing RNA-RNA interaction RNA-RNA interactions RNAs SARS-CoV-2 SARS-CoV-2 genome SARS-CoV-2 viral secondary structure Sequencing small nucleolar RNA structures subgenomic RNA subgenomic RNAs targeted therapy the SARS-CoV-2 genome untranslated regions Viral viral genome virus virus genome virus infection Virus-host interaction Virus-host interactions wildtype Δ382 [DOI] 10.1038/s41467-021-25357-1 PMC 바로가기 [Article Type] Article
Clinical and epidemiologic characteristics of inconclusive results in SARS-CoV-2 RT-PCR assaysSARS-COV-2 RT-PCR 분석에서 결정적인 결과의 임상 및 역학적 특성Research Published on 2021-08-212022-09-10 Journal: BMC Infectious Diseases [Category] MERS, SARS, 치료기술, [키워드] addition amplified Characteristics clinical Clinical significance clinician confirmed case Confusion COVID-19 detect Follow-up Inconclusive Inconclusive result Infection prevention infections molecular Molecular assay PCR assays positive result Prevention and control respiratory respiratory specimen respiratory specimens Result RNA RT-PCR RT-PCR assay SARS-CoV-2 SARS-CoV-2 RT-PCR sgRNA sgRNAs specimen specimens subgenomic RNA supplementary material Viral [DOI] 10.1186/s12879-021-06534-5 PMC 바로가기 [Article Type] Research
SARS-CoV-2: from its discovery to genome structure, transcription, and replicationReview Published on 2021-07-192022-11-01 Journal: Cell & Bioscience [Category] COVID-19, [키워드] abundance ACE2 acute respiratory syndrome Atypical cleaved complex conducted contagious coronavirus COVID-19 COVID-19 pandemic Efficiency ENCODE exhibit expressing full-length function Genetic Genome genome structure genomic RNA gRNA infected cell infected cells Interaction isolates motif mRNA non-structural protein Nsp12 nsp13 Nsp9 ORF ORF1a ORF1b Pneumonia polyprotein positive-sense protease Proteins Replication respiratory virus responsible RNA RNA primer RNA synthesis RTC SARS-CoV-2 SARS-CoV-2 genome sgRNA sgRNAs single-stranded subgenomic RNA susceptible cell synthesis the SARS-CoV-2 genome TMPRSS2 Transcription transcription regulatory sequence translate translation TRS upstream Vesicle viral genome viral genome replication viral replication [DOI] 10.1186/s13578-021-00643-z PMC 바로가기 [Article Type] Review
ddPCR increases detection of SARS-CoV-2 RNA in patients with low viral loadsDDPCR은 바이러스 부하가 낮은 환자에서 SARS-COV-2 RNA의 검출을 증가시킵니다.Original Article Published on 2021-07-122022-09-10 Journal: Archives of Virology [Category] SARS, 유전자 메커니즘, 진단, 치료기술, 치료법, 치료제, [키워드] airway Cell coding concerning COVID-19 diagnose Diagnosis digital PCR droplet expressed framework French Helicase hospital in vitro increase Infection investigated Molecular diagnosis MONITOR N gene N or E Nasopharyngeal swab nasopharyngeal swabs pandemic Paris Patient PCR positive proportion qPCR RdRP Region replicative RNA RNA sequence RNA sequences RT-qPCR SARS-CoV-2 SARS-CoV-2 RNA scored sensitivity significantly subgenomic RNA supplementary material targets Undetermined upper airway Viral load [DOI] 10.1007/s00705-021-05149-0 PMC 바로가기 [Article Type] Original Article
SARS-CoV-2 Subgenomic RNA Kinetics in Longitudinal Clinical SamplesMajor Articles Published on 2021-07-112022-10-29 Journal: Open Forum Infectious Diseases [Category] COVID-19, [키워드] A549 acute respiratory syndrome analyzed Cell clinical clinical sample clinical samples clinically Cohort collected coronavirus coronavirus disease correlated COVID-19 cycle Decay kinetics detectable dimethylsulfoxide Favipiravir genomic RNA gRNA in vitro infected with SARS-CoV-2 infectiousness Kinetics Lambda longitudinal marker N-gene not differ Participants patients patients with COVID-19 persistence placebo-controlled polymerase chain potential molecular quantified Quantitative R 2 remained Remdesivir reported Result reverse transcription RNA Sample SARS-CoV-2 sgRNA sgRNAs significantly subgenomic RNA Support Swab symptom onset threshold treated Treatment Trial trials viability Viral RNA [DOI] 10.1093/ofid/ofab310 PMC 바로가기 [Article Type] Major Articles
Frontline Screening for SARS-CoV-2 Infection at Emergency Department Admission by Third Generation Rapid Antigen Test: Can We Spare RT-qPCR?3세대 신속 항원 검사를 통한 응급실 입원 시 SARS-CoV-2 감염에 대한 최전선 검사: RT-qPCR을 절약할 수 있습니까?Article Published on 2021-05-012022-09-01 Journal: Viruses [Category] SARS, 진단, 치료기술, [키워드] 95% CI active viral replication acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Admission antigenic test Can Clinical use complement coronavirus country COVID-19 cycle threshold demonstrated Department Diagnosis diagnostic digital PCR droplet Emergency emergency department expected false-negative Generation genomic hospital increase infections Infectivity introduced IQR lack median Nasopharyngeal swab nasopharyngeal swabs Patient PCR persists qPCR quantification Rapid Rapid antigen tests Rapid diagnostic test respiratory risk RNA RT-qPCR SARS-CoV-2 SARS-CoV-2 RNA Screening sensitivity severe acute respiratory syndrome Coronavirus specificity subgenomic RNA subject suggested the WHO Viral Viral load [DOI] 10.3390/v13050818 PMC 바로가기 [Article Type] Article
Combined RT-qPCR and pyrosequencing of a Spike glycoprotein polybasic cleavage motif can uncover pediatric SARS-CoV-2 infections associated with heterogeneous presentationResearch Published on 2021-04-242022-10-30 Journal: Molecular and Cellular Pediatrics [Category] COVID-19, [키워드] (+)RNA acute respiratory syndrome Affect Antibody testing applied bandwidth biotinylated Blood children cleavage clinically Combined containment measures coronavirus Course COVID-19 diagnosis COVID-19 surveillance deep sequencing Detection limit diagnostic diagnostics Diseases Epidemiology exhibiting expand false-negative Genome glycoprotein gold standard help heterogeneous Hospitalized implementation Inflammatory Interpretation Laboratory Mild MIS-C motif P681H Patient pediatric pediatric cohort Pediatric SARS-CoV-2-associated diseases polybasic polymerase chain primer protocol pyrosequencing qPCR Quantitative Result RT-qPCR S-gene SARS-CoV-2 SARS-CoV-2 B.1.1.7 SARS-COV-2 infection sgRNAs specimen spike subgenomic RNA subsequent supplementary material symptom onset syndrome tested Transcription transmission of SARS-CoV-2 variants of concern Viral load [DOI] 10.1186/s40348-021-00115-x PMC 바로가기 [Article Type] Research
A Negative Feedback Model to Explain Regulation of SARS-CoV-2 Replication and TranscriptionGenetics Published on 2021-02-262022-10-31 Journal: Frontiers in Genetics [Category] COVID-19, [키워드] acute respiratory syndrome caused cleavage site cleaving comprehensive analysis Control coronavirus Coronavirus disease 2019 CoV CoV replication COVID-19 CoVs derivatives drug explain expression level feedback Gene Expression genome sequence genomic RNA hosts influence knowledge mechanism Model molecular basis motif negative Nsp15 persistence proportion Protein regulate regulated Regulation regulation model Replication Result RNA SARS-CoV-2 sgRNA sgRNAs subgenomic RNA Transcription transcription regulatory sequence TRS TRSs turn uridine [DOI] 10.3389/fgene.2021.641445 PMC 바로가기 [Article Type] Genetics
Comparison of Subgenomic and Total RNA in SARS-CoV-2-Challenged Rhesus MacaquesVaccines and Antiviral Agents Published on 2021-01-202022-10-30 Journal: Journal of Virology [Category] COVID-19, [키워드] administration anatomical antiviral drugs cell entry convalescent Critical Developing Efficacy genomic RNA gRNA in vivo inactivated infected cell Infection lysates macaque marker MONITOR monoclonal antibodies neutralized non-human primates nucleocapsid positive Prophylactic Protective public health remained replicating virus respiratory respiratory virus reverse transcription PCR rhesus macaque rhesus macaques RNA RNase RT-PCR RT-PCR assay SARS-CoV-2 SARS-CoV-2 virus sgRNA subgenomic RNA the SARS-CoV-2 virus therapeutic therapeutic efficacy Total total RNA utility Vaccines Viral load virions virus [DOI] 10.1128/JVI.02370-20 PMC 바로가기 [Article Type] Vaccines and Antiviral Agents