Comparative evaluation of flavonoids reveals the superiority and promising inhibition activity of silibinin against SARS-CoV-2플라보노이드의 비교 평가는 SARS-CoV-2에 대한 silibinin의 우수성과 유망한 억제 활성을 나타냅니다Article Published on 2022-07-012022-09-11 Journal: Phytotherapy research : PTR [Category] COVID19(2023년), SARS, 치료제, [키워드] amino acids antiviral activity binding binding affinity Comparative Compound enzyme Enzyme inhibition evaluated exhibited FDA-approved drug flavonoid flavonoids followed by highest highest binding affinity in silico in silico study in vitro in vitro evaluation in vitro study indicated inhibition activity inhibition assay Interaction interfere key amino acid key amino acids M pro mammalian cell mapping molecular docking plant plants protease Protein RdRP Replication reveal S protein Safe SARS-CoV-2 SARS-CoV-2 entry SARS-CoV-2 protein SARS-CoV-2 S protein Screening selectivity index Serine significantly silibinin silibinin. systematic literature search targets TMPRSS2 transmembrane serine protease virucidal was tested [DOI] 10.1002/ptr.7486 PMC 바로가기 [Article Type] Article
Broadly Applicable, Virus-Free Dual Reporter Assay to Identify Compounds Interfering with Membrane Fusion: Performance for HSV-1 and SARS-CoV-2막 융합을 방해하는 화합물을 식별하기 위해 광범위하게 적용 가능한 바이러스 없는 이중 보고자 분석: HSV-1 및 SARS-CoV-2에 대한 성능Article Published on 2022-06-212022-09-11 Journal: Viruses [Category] COVID19(2023년), SARS, 치료기술, 치료제, [키워드] alkaline alkaline phosphatase antiviral agent Antiviral agents cell-cell fusion cell–cell fusion Compound doxycycline dual reporter assay Efficacy enzyme expressed expression fluorescent fusion inhibition HSV-1 identify inhibitors Internal control marker membrane fusion nanoluciferase nuclear Pathogens performance phosphatase quantification Replication reported reporter reporter fusion reporter gene SARS-CoV-2 secreted secreted alkaline phosphatase secreted nanoluciferase. Sensitivity and specificity Tetracycline transient transfection Vero Cell Vero cells viral pathogens was tested [DOI] 10.3390/v14071354 PMC 바로가기 [Article Type] Article
Developing Pseudovirus-Based Neutralization Assay against Omicron-Included SARS-CoV-2 Variants오미크론 포함 SARS-CoV-2 변이체에 대한 의사 바이러스 기반 중화 분석 개발Article Published on 2022-06-182022-09-11 Journal: Viruses [Category] COVID19(2023년), MERS, SARS, 변종, 진단, [키워드] Alpha antibodies approved backbone Beta biosafety BSL-2 candidate collection time conditions conducted convalescent Convalescent patients convalescent plasma COVID-19 convalescent patient COVID-19 pandemic Developing drugs effective Efficiency expression plasmid full-length Gamma Genome Health HIV HIV-1 immune evasion in vitro luciferase reporter gene monoclonal antibodies neutralization Neutralization assay Neutralizing antibodies neutralizing capacity omicron parameter Particles Plasmid Prevention and control Protein pseudovirus Pseudovirus neutralization assay pseudoviruses reduced REGN10933 REGN10987 reporter gene S protein expression plasmids S309 SARS-CoV-2 SARS-CoV-2 variant SARS-CoV-2 variants Spread the S protein therapeutic Vaccine Vaccines variant was tested Wuhan [DOI] 10.3390/v14061332 PMC 바로가기 [Article Type] Article
Clinical evaluation of the Diagnostic Analyzer for Selective Hybridization (DASH): A point-of-care PCR test for rapid detection of SARS-CoV-2 infectionDASH(Diagnostic Analyzer for Selective Hybridization) 임상 평가: SARS-CoV-2 감염의 신속한 검출을 위한 현장 진료 PCR 검사Article Published on 2022-06-162022-09-11 Journal: PLoS ONE [Category] COVID19(2023년), SARS, 치료기술, [키워드] analyzed Analyzer Antigen calculated Cepheid clinical clinical setting collected Concordance conducted correlated COVID-19 COVID-19 symptoms COVID-19 vaccine cross-sectional cross-sectional study DASH dose doses enrolled evaluated female gold gold standard hybridization information Laboratory median age mRNA nasal swab negative and positive Negative predictive value participant PCR PCR test performed POC point-of-care positive Positive predictive value Predictive Predictive value provided SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 PCR sensitivity specificity speed Swab symptomatic symptoms of COVID-19 vaccination was tested without symptom [DOI] 10.1371/journal.pone.0270060 PMC 바로가기 [Article Type] Article
Assessment of antiphospholipid antibodies profiles based on severity of COVID-19 pneumoniaArticle Published on 2022-06-092022-11-15 Journal: The Pan African Medical Journal [Category] 진단, [키워드] antibodies antibody Anticoagulant anticoagulation Antiphospholipid APL assessment association clinical impact conducted coronavirus disease COVID-19 COVID-19 group COVID-19 patient COVID-19 patients Critical Critically ill ELISA enzyme-linked immunosorbent event Factor Frequency highest hospitalized patient ICU IgA IgM intensive care involved lupus anticoagulant mechanism no significant difference non-ICU Patient patients Pneumonia positive predominant profile profiles Prophylactic Retrospective study severe complication severity of COVID-19 tested thrombosis thrombotic Thrombotic events thrombotic risk was tested [DOI] 10.11604/pamj.2022.42.110.33020 PMC 바로가기
Machine learning prediction of 3CL pro SARS-CoV-2 docking scores3CL pro SARS-CoV-2 도킹 점수의 기계 학습 예측Article Published on 2022-06-012022-09-11 Journal: Computational biology and chemistry [Category] COVID19(2023년), SARS, 신약개발, 치료제, [키워드] 3CL pro 3CLpro 3CLpro Mpro 6WQF addition approach approaches AutoDock AutoDock molecular docking Compound COVID19 docking result docking score docking scores Final Gradient in vivo Kera machine machine learning molecular MPro Neural position predicted Randomly SARS-CoV-2 selected Smooth subsequent TensorFlow TensorFlow XGBoost SchNetPack. was tested were used Zinc [DOI] 10.1016/j.compbiolchem.2022.107656 PMC 바로가기 [Article Type] Article
Interference of Chaga mushroom terpenoids with the attachment of SARS-CoV-2; in silico perspectiveSARS-CoV-2의 부착으로 차가버섯 테르페노이드의 간섭; 실리코 관점에서Article Published on 2022-06-012022-09-11 Journal: Computers in biology and medicine [Category] COVID19(2023년), SARS, 변종, 신약개발, [키워드] 501.V2 affinities approach binding affinities binding affinity binding energy can be used cancer cell Cancer cells Chaga terpenoids compounds COVID-19 domain drug finding Glucose GRP78 host cell host cell receptor HSPA5 in silico in vivo in-silico inhibitor interfere Interference investigator MM-GBSA molecular docking molecular dynamics Molecular dynamics simulation molecular dynamics simulations NAMD pandemic peptide performed Perspective Protein receptor receptors Recognition reported Result SARS-CoV-2 SARS-CoV-2 receptor targeting terpenoid tested the binding affinity the SARS-CoV-2 therapeutic variants variants of SARS-CoV-2 was tested [DOI] 10.1016/j.compbiomed.2022.105478 PMC 바로가기 [Article Type] Article
Lysozyme Protects Against Severe Acute Respiratory Syndrome Coronavirus 2 Infection and Inflammation in Human Corneal Epithelial Cells리소자임은 인간 각막 상피 세포의 중증 급성 호흡기 증후군 코로나바이러스 2 감염 및 염증으로부터 보호합니다Article Published on 2022-06-012022-09-11 Journal: Investigative ophthalmology & visual science [Category] COVID19(2023년), SARS, 치료기술, 치료제, [키워드] ACE2 acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 addition Against analyzed angiotensin Angiotensin-converting enzyme angiotensin-converting enzyme 2 cell entry Cell toxicity characterized Corneal coronavirus coronavirus 2 Effect entry of SARS-CoV-2 enzyme epithelial cell epithelial cells expressed expression Human IL-6 Infection Inflammatory cytokine Inflammatory response luciferase lysozyme MCP-1 Pathogens pro-inflammatory molecule pro-inflammatory molecules proinflammatory molecule proinflammatory molecules PROTECT protective effect Protective effects pseudotyped respiratory robust RT-PCR SAR-CoV-2 SARS-CoV-2 SARS-COV-2 infection secretion Serine Spike protein Spike proteins stimulated suggested susceptibility tears TMPRSS2 TNF-α Toxicity transmembrane serine protease was measured was tested western blotting [DOI] 10.1167/iovs.63.6.16 PMC 바로가기 [Article Type] Article
The use of a surgical helmet system with a high-efficiency particulate air filter as possible protection equipment during the coronavirus disease 2019 pandemic: a double-blinded randomized control studyRandomized Controlled Trial Published on 2022-06-012022-10-04 Journal: International Orthopaedics [Category] COVID19(2023년), SARS, 임상, [키워드] aerosol Aerosol generating procedures cause Cohort cohorts coronavirus disease Coronaviruses COVID-19 double-blinded droplet droplets Efficacy element equipment Filter FIVE groups Hood Innovation marker Mask microorganisms participant Patient Personal protective equipment Placebo placebo group positive PROTECT Protective qualitative test random randomized control study randomized controlled study recruited reported Respirator Respiratory droplets saccharin Safe SARS-CoV-2 Spread statistically significant difference surgeon surgical surgical mask sweet Total virus was performed was tested [DOI] 10.1007/s00264-022-05371-8 PMC 바로가기 [Article Type] Randomized Controlled Trial
Analysis of SARS-CoV-2 known and novel subgenomic mRNAs in cell culture, animal model, and clinical samples using LeTRS, a bioinformatic tool to identify unique sequence identifiers고유한 서열 식별자를 식별하는 생물정보학 도구인 LeTRS를 사용하여 세포 배양, 동물 모델 및 임상 샘플에서 SARS-CoV-2 알려진 및 신규 서브게놈 mRNA 분석Article Published on 2022-05-262022-09-11 Journal: GigaScience [Category] COVID19(2023년), SARS, 변종, 유전자 메커니즘, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus animal model animal models bioinformatics Biology biology of SARS-CoV-2 can be used Cell Cell culture clinical sample clinical samples complex coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 data set diagnostics Direct RNA sequencing Each evaluate generate host response identify implication in some indicated Infection junction longitudinal samples mammalian mechanism medical countermeasure Medical countermeasures Middle East Middle East respiratory syndrome Coronavirus multiphasic Nanopore nasopharyngeal sample nucleic acid open reading frames other coronavirus other coronaviruses Patient plasticity provide Quantitative reflected Regulatory respiratory syndrome coronavirus RNA RNA modification SARS-CoV-2 selection pressure sequence Sequencing sequencing data severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 sgmRNA sgmRNAs subgenomic mRNA subject target targets Transcription transcriptional regulatory sequences transcriptional regulatory sequences. Transmission model unique variant Viral RNA viral RNA synthesis virus was tested [DOI] 10.1093/gigascience/giac045 PMC 바로가기 [Article Type] Article