Preparation and characterization of a novel monoclonal antibody specific to severe acute respiratory syndrome-coronavirus nucleocapsid proteinArticle Published on 2006-08-302022-11-01 Journal: Virus research [Category] Coronavirus, [키워드] Antigen assays C-terminus Cell coronavirus cross-reactivity Diagnosis diagnostic early diagnosis epitope expressed FIVE found generate Host IgG immune Infection insect cell linear epitopes mAb modulation monoclonal antibody N protein N proteins N-terminus nucleocapsid nucleocapsid protein pathology phosphorylated preparation Protein reactivity related SARS-CoV SARS-CoV N selected severe Severe acute respiratory syndrome suggested viral pathogenesis virus replication [DOI] 10.1016/j.virusres.2006.07.004 PMC 바로가기 [Article Type] Article
Generation of human antibody fragments recognizing distinct epitopes of the nucleocapsid (N) SARS-CoV protein using a phage display approachResearch Article Published on 2005-09-192022-10-28 Journal: BMC Infectious Diseases [Category] Coronavirus, [키워드] acute respiratory syndrome antibody antibody fragment Antiviral compound approach cause characterized coated with antigen described detect diagnostic test disease domain ELISA epitope Generation high mortality rate Human immunocytochemistry immunohistochemistry in vitro Infected Isolation MONITOR N protein neutralizing antibody nucleocapsid Particle Prevent Protein reagent recognize recognizing Result SARS epidemic SARS virus SARS-CoV SARS-CoV N protein scFv selected severe Spread target cells treat Vaccine Vero cells virus was used [DOI] 10.1186/1471-2334-5-73 PMC 바로가기 [Article Type] Research Article
G0/G1 arrest and apoptosis induced by SARS-CoV 3b protein in transfected cellsShort Report Published on 2005-08-172022-10-28 Journal: Virology Journal [Category] Coronavirus, [키워드] amino acids Analysis Annexin Apoptosis apoptosis induction Atypical Cell cell cycle arrest cells apoptosis characterized coronavirus COS-7 cell COS-7 cells ENCODE expression found fusion protein Genome immune induce infect labeling life-threatening liver lung mechanism Necrosis non-structural protein non-structural proteins organ organs over-expression Pathogenesis Pneumonia predicted Protein Proteins related Replication respiratory SARS pathogenesis SARS-CoV severe similarity structural protein Till transfected cell Vero virulence viruses [DOI] 10.1186/1743-422X-2-66 PMC 바로가기 [Article Type] Short Report
SARS Vaccine DevelopmentSynopsis Published on 2005-07-012022-10-31 Journal: Emerging Infectious Diseases [Category] Coronavirus, [키워드] acute respiratory syndrome can be used Clinical use conformational coronavirus develop Developing development effective inactivated inducer Infection Neutralizing antibodies Neutralizing epitone neutralizing epitopes Prevent Protein RBD Receptor-binding domain Recombinant protein S protein Safe SARS SARS-CoV SARS-CoV vaccine sequence Spike protein the RBD the receptor-binding domain the S1 subunit Vaccine Vaccines vector [DOI] 10.3201/eid1107.050219 PMC 바로가기 [Article Type] Synopsis
Pseudoknots: RNA Structures with Diverse FunctionsPrimer Published on 2005-06-142022-10-28 Journal: PLoS Biology [Category] Coronavirus, [키워드] adopted function motifs prevalent Protein RNA RNA molecules Structure [DOI] 10.1371/journal.pbio.0030213 PMC 바로가기 [Article Type] Primer
Development of a homogeneous screening assay for automated detection of antiviral agents active against severe acute respiratory syndrome-associated coronavirusArticle Published on 2005-06-142022-11-01 Journal: Journal of virological methods [Category] Coronavirus, [키워드] 2003 active against Antiviral antiviral activity antiviral agent Antiviral compounds antiviral drug antiviral potency assay automated be applied compounds coronavirus cytotoxicity demonstrated detect development Drug discovery EGFP expression evaluated Express facilitate fluorescent gastroenteritis Health High-throughput screening homogeneous inhibitor initial screening outbreak Protein risk SARS-CoV SARS-CoV infection severity specificity Spread therapeutic agent viral disease virus viruses [DOI] 10.1016/j.jviromet.2005.05.010 PMC 바로가기 [Article Type] Article
Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infectionsResearch Published on 2005-02-102022-10-28 Journal: Virology Journal [Category] Coronavirus, [키워드] Analysis binding Camelpox cell line cellular compared criteria cytomegalovirus determined experiment expression expression level Gene Human Infected infected cells Infection mRNA expression Protein quantitative real-time PCR ranking Real-time PCR reference reference gene reported SARS Coronavirus tested viral replication virus were infected yellow fever β-actin [DOI] 10.1186/1743-422X-2-7 PMC 바로가기 [Article Type] Research
The Severe Acute Respiratory Syndrome (SARS)-coronavirus 3a protein may function as a modulator of the trafficking properties of the spike proteinHypothesis Published on 2005-02-102022-10-28 Journal: Virology Journal [Category] Coronavirus, [키워드] addition biochemically Cell Coronaviruses cytoplasmic cytoplasmic tail decrease different domain Effect examined expressed expression genetics Genome homologue Hypothesis Impact in vitro in vivo infected cell Infection Interaction mechanism methodology modulate motif Open reading frame other coronaviruses pathway presentation Protein Replication reported respiratory resulting RNA SARS-CoV severe shown significantly Spike protein Testing the spike protein undergo unique viral infection viral pathogenesis Viral protein viral spread [DOI] 10.1186/1743-422X-2-5 PMC 바로가기 [Article Type] Hypothesis
The Structure of a Rigorously Conserved RNA Element within the SARS Virus GenomeResearch Article Published on 2005-01-282022-10-28 Journal: PLoS Biology [Category] Coronavirus, [키워드] 3′ end absence Anti-viral approach bind Comparisons component conserved coronavirus element explained extreme feature function Genome homology Host host cell host protein Human genome in viral include Interaction interactions interior ions macromolecular mechanism motif mutable occur pathogen pathogenic Protein protein synthesis reactive related required ribosomal RNA RNA RNA genome RNA genomes SARS virus Sequence conservation Structure target therapeutic agents unique Viral RNA virus [DOI] 10.1371/journal.pbio.0030005 PMC 바로가기 [Article Type] Research Article
Nucleocapsid Protein as Early Diagnostic Marker for SARSDispatch Published on 2004-11-012022-10-31 Journal: Emerging Infectious Diseases [Category] Coronavirus, [키워드] acute respiratory syndrome can be used coronavirus diagnostic early N protein nucleocapsid nucleocapsid protein, monoclonal antibody, enzyme-linked immunosorbent assay, early diagnosis, severe acute respiratory syndrome, dispatch Patient Protein SARS-CoV sensitivity serum sample specificity tested [DOI] 10.3201/eid1011.040516 PMC 바로가기 [Article Type] Dispatch