Whole-Genome Sequence of SARS-CoV-2 Isolate Siena-1/2020Genome Sequences Published on 2020-09-242022-10-29 Journal: Microbiology Resource Announcements [Category] COVID-19, [키워드] acute respiratory syndrome Amino acid Amplicon sequencing approaches coding sequence Complete coronavirus D614G Direct RNA sequencing Europe genome sequence isolate Lineage Mutation nanopore sequencing prevalent SARS-CoV-2 the spike protein was obtained [DOI] 10.1128/MRA.00944-20 PMC 바로가기 [Article Type] Genome Sequences
A Comparison of Whole Genome Sequencing of SARS-CoV-2 Using Amplicon-Based Sequencing, Random Hexamers, and Bait CaptureArticle Published on 2020-08-152022-10-28 Journal: Viruses [Category] COVID-19, MERS, [키워드] acute respiratory syndrome adaptive addition Amplicon sequencing amplification Analysis analyzed approaches assembly bait capture bioinformatics capture cDNA clinical sample Concentration concentrations Consensus coronavirus Coverage diagnostics disadvantage ecosystem effective Efficacy Evolution examined facilitated genome coverage Genome sequencing highest Illumina increasingly MONITOR Multiplex PCR PCR performed polymerase chain protocol protocols provided SARS-CoV-2 SARS-CoV-2 cDNA SARS-CoV-2 genome sequence sequenced Sequencing the SARS-CoV-2 genome Transmission Viral RNA virus while [DOI] 10.3390/v12080895 PMC 바로가기 [Article Type] Article
Integrated genomic view of SARS-CoV-2 in IndiaResearch Article Published on 2020-08-032022-10-28 Journal: Wellcome Open Research [Category] COVID-19, [키워드] A2a Amplicon sequencing Analysis Asia Cluster Combination condition country COVID COVID-19 Europe Genome genomic genomic data Haplotype host response India Indonesia Local Malaysia management Middle East MinION molecular occurred outbreak Oxford pandemic persistence prevalent SARS-CoV-2 SARS-CoV-2 genomes Sequencing Surveillance technology Transmission triggered USA viral genomes Whole genome sequencing Wuhan, China [DOI] 10.12688/wellcomeopenres.16119.1 PMC 바로가기 [Article Type] Research Article
Evaluation of NGS-based approaches for SARS-CoV-2 whole genome characterisationRapid Communication Published on 2020-07-052022-10-28 Journal: Virus Evolution [Category] COVID-19, [키워드] Amplicon sequencing amplicon-based enrichment approach ARTIC Network bioinformatics capture method characterisation Complete consensus sequence Coverage COVID-19 COVID-19 outbreak DNA enrichment evaluated Genome genome coverage genomes Genomic surveillance Genomics identity Illumina metagenomic metagenomic sequencing next generation sequencing NGS ONT ORF6 Oxford Nanopore Technology Panel performed producing protocol reference sequence Region required respiratory respiratory sample SARS-CoV-2 sequenced Sequencing SNP threshold Viral load WGS whole-genome sequencing [DOI] 10.1093/ve/veaa075 PMC 바로가기 [Article Type] Rapid Communication
Multiple approaches for massively parallel sequencing of SARS-CoV-2 genomes directly from clinical samples임상 샘플에서 직접 SARS-CoV-2 게놈의 대규모 병렬 시퀀싱을 위한 여러 접근 방식Research Published on 2020-06-302022-08-13 Journal: Genome Medicine [Category] SARS, 유전자 메커니즘, [키워드] (coronavirus disease 2019 Accuracy acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Amplicon amplicon method Amplicon sequencing analyses Analysis applied approach approaches best capture capture method caused Characteristics clinical sample clinical samples Complete coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 cultured isolate demonstrated diagnostics Dilution Efficiency eight Emerging infectious diseases Epidemic Epidemiology Evolution examined Factor full-length SARS-CoV-2 genetic information Genome Genome sequencing genomes Genomic surveillance Guidance Hybrid capture inter- and intra-individual variations inter-individual inter-individual and intra-individual variations intra-host dynamics intra-individual variation iSNV knowledge Metatranscriptomic sequencing Multiple Multiplex PCR novel multiplex PCR amplicon offer PCR amplicon Quasispecies reported respiratory Result SARS-CoV-2 SARS-CoV-2 genome scarcity sensitivity Sequencing severe acute respiratory syndrome Coronavirus Therapeutics ultra-high-throughput metatranscriptomic variations Viral Viral load viral loads viral read Viral transmission virus Virus evolution Virus-host interaction Virus-host interactions viruses whole-genome sequencing [DOI] 10.1186/s13073-020-00751-4 PMC 바로가기 [Article Type] Research
Fitness Costs and the Rapid Spread of kelch13 -C580Y Substitutions Conferring Artemisinin Resistance피트니스 비용과 아르테미시닌 저항성을 부여하는 kelch13 -C580Y 치환의 빠른 확산Article Published on 2018-06-182024-08-11 Journal: Med Instrum [Category] 말라리아, [키워드] Amplicon sequencing CRISPR/Cas9 fitness costs selection coefficient [DOI] 10.1128/AAC.00605-18 PMC 바로가기
A metagenomic viral discovery approach identifies potential zoonotic and novel mammalian viruses in Neoromicia bats within South AfricaResearch Article Published on 2018-03-262022-10-28 Journal: PLoS ONE [Category] Coronavirus, Fulltext, 조류인플루엔자, [키워드] Adenoviridae Adenovirus African Amplicon sequencing Analysis anthropogenic approach bat bats Circoviridae coding collected Complete conserved Contact Conventional Coronaviridae coronavirus Coronaviruses Coverage determine eight fecal FIVE gene region generate genetic diversity Genome Herpesviridae highlighting Host hosts identify identity Illumina Miseq intestinal tissue lack library mammalian metagenomic metagenomic sequencing Middle East MiSeq nucleotide Papillomaviridae Parvoviridae PCR analysis PCR assay platform Population producing rectal Region regions Sample sequence sequenced sequencing data sequencing depth South Africa Specy Structure syndrome viral family Viral particles virus was determined were used widespread zoonotic [DOI] 10.1371/journal.pone.0194527 PMC 바로가기 [Article Type] Research Article
Using Amplicon Deep Sequencing to Detect Genetic Signatures of Plasmodium vivax RelapseArticle Published on 2015-09-152024-08-29 Journal: Nanoscale [Category] 말라리아, [키워드] Amplicon sequencing deep sequencing genetic diversity hypnozoite malaria Microsatellite multiplicity of infection Plasmodium vivax Pvmsp1 Relapse