Expression of SARS-CoV-2 surface glycoprotein fragment 319-640 in E. coli, and its refolding and purification대장균에서 SARS-CoV-2 표면 당단백질 단편 319-640의 발현 및 이의 리폴딩 및 정제Article Published on 2021-07-012022-09-11 Journal: Protein expression and purification [Category] SARS, 유전자 메커니즘, 치료기술, 치료제, [키워드] antibody Antibody test Antigen antigenic Bacterial expression binding cells cellular receptor COVID-19 COVID-19 immunity detect determine domain E. coli Efficacy epidemiological expressed expression fragment human cells include Inclusion predict presence of antibody public health purification RBD Receptor-binding domain resulting SARS-CoV-2 sensitive serology test serology tests solubilization spike Surface glycoprotein Vaccine vaccine-induced immune response viral entry [DOI] 10.1016/j.pep.2021.105861 PMC 바로가기 [Article Type] Article
Making sense of spike D614G in SARS-CoV-2 transmissionSARS-CoV-2 전송에서 스파이크 D614G 이해하기Review Published on 2021-07-012022-09-11 Journal: Science China. Life sciences [Category] SARS, 변종, [키워드] ACE2 acute respiratory syndrome acute respiratory syndrome coronavirus adaptive Amino acid amino acid position angiotensin angiotensin converting enzyme angiotensin converting enzyme 2 antibody asparagine binding cellular receptor circulating coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 D614G D614G mutation determine dominant Efficacy ENhance enzyme etiologic agent function global pandemic glycine Host human host in viral Making Mutation pandemic Pathogenesis Replication SARS-CoV-2 SARS-CoV-2 transmission severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 Spike protein therapeutic tissue tropism Transmission Vaccine variants viral evolution viral fitness viral replication viral spike protein virus strains [DOI] 10.1007/s11427-020-1893-9 PMC 바로가기 [Article Type] Review
Up State of the SARS-COV-2 Spike Homotrimer Favors an Increased Virulence for New VariantsMedical Technology Published on 2021-06-302022-11-01 Journal: Frontiers in Medical Technology [Category] COVID-19, [키워드] ACE2 alteration Analysis Angiotensin-converting enzyme antibody production B-cell B.1.351 binding affinity carried cellular receptor Combination conformational state conformational states contribute coronavirus COVID-19 pandemic disease dose Efficiency electrostatic interactions epitope Epitopes explain Hospitalization Increased Infection Interaction mapped molecular mechanism Mutation mutations New protein–protein interactions raising RBD RBD-ACE2 released responsible S trimer SARS-CoV-2 South spike Spread stability state Structure the receptor-binding domain the SARS-CoV-2 the vaccine Therapeutic molecule therapeutic option Transmissibility trimeric variant variants variants of SARS-CoV-2 virulence virulent virus wild type [DOI] 10.3389/fmedt.2021.694347 PMC 바로가기 [Article Type] Medical Technology
Adaptation of the endemic coronaviruses HCoV-OC43 and HCoV-229E to the human hostResearch Article Published on 2021-06-242022-10-28 Journal: Virus Evolution [Category] COVID-19, [키워드] accessory proteins accompanied accumulated adaptation antigenic antigenic drift applied approach binding affinity both virus both viruses cellular receptor class coronavirus Coronaviruses driven by Endemic endemic coronavirus event Evolution evolutionary dynamics generate HCoV-229E HCoV-HKU1 HCoV-NL63 HCoV-OC43 HCoV-OC43 spike Hemagglutinin-esterase higher affinity highly pathogenic human cell human host human populations Hypothesis I, II Immunity indicated Infection Like Limited genetic diversity Membrane proteins molecular dating molecular evolution much higher Positive selection Protein RBD RBDs Receptor binding Result SARS-CoV-2 evolution selected selective Spike protein strain suggested targets the RBD the receptor-binding domain the spike protein time interval trajectory undergo virus zoonotic zoonotic origins [DOI] 10.1093/ve/veab061 PMC 바로가기 [Article Type] Research Article
N-Glycosylation of the SARS-CoV-2 Receptor Binding Domain Is Important for Functional Expression in PlantsPlant Science Published on 2021-06-152022-10-31 Journal: Frontiers in Plant Science [Category] COVID-19, [키워드] ACE2 Amino acid antibody benthamiana binding cellular receptor characterized complex convalescent sera COVID-19 CR3022 enzyme expressed glycoprotein glycosylated glycosylation growth factor had no Host monoclonal antibodies monomer N-glycan N-glycosylation Nicotiana benthamiana plant posttranslational modification Protein protein expression RBD Receptor binding domain Recombinant protein recombinant proteins SARS-CoV-2 secreted site-directed mutagenesis Spike protein the SARS-CoV-2 variant Variation Viral antigen virus while [DOI] 10.3389/fpls.2021.689104 PMC 바로가기 [Article Type] Plant Science
A Bioluminescent Biosensor for Quantifying the Interaction of SARS-CoV-2 and Its Receptor ACE2 in Cells and In VitroArticle Published on 2021-06-022022-10-28 Journal: Viruses [Category] COVID-19, SARS, [키워드] ACE2 acute respiratory syndrome angiotensin-converting enzyme 2 antibodies bioluminescent bioluminescent biosensor biosensor cellular receptor Coronavirus-2 COVID-19 develop Drug discovery drugs evaluate evaluated in vitro Interaction living cell mutant mutant virus NanoBiT peptide RBD Receptor binding domain SARS-CoV-2 SARS-CoV-2 RBD significantly small molecule the SARS-CoV-2 therapeutic drugs virus–host interaction [DOI] 10.3390/v13061055 PMC 바로가기 [Article Type] Article
Variation in predicted COVID-19 risk among lemurs and lorises여우원숭이와 로리스의 예측된 COVID-19 위험의 변동Article Published on 2021-06-012022-09-11 Journal: American journal of primatology [Category] 변종, [키워드] ACE2 ACE2 gene ACE2 sequence Amino acid Analysis angiotensin angiotensin-converting enzyme-2 anticipated caused cellular receptor clade COVID-19 effort Endemic example exposure to extinction fatality feasible group Health high risk high variability highest Human in some Infection infection rate infection rates limit locus Novel coronavirus novel coronavirus SARS-CoV-2 predict predicted primates protein sequence protein sequences recent rising risk SARS-CoV-2 SARS-COV-2 infection sequence susceptibility susceptible the disease Variability variants Variation [DOI] 10.1002/ajp.23255 PMC 바로가기 [Article Type] Article
Animal models for SARS-Cov2/Covid19 research-A commentarySARS-Cov2/Covid19 연구용 동물 모델-A 논평Review Published on 2021-06-012022-09-11 Journal: Biochemical pharmacology [Category] SARS, 신약개발, 유전자 메커니즘, 치료제, [키워드] ACE2 addition animal animal model BALB/c cellular receptor clearance clinical feature Clinical features Co-infection Combination COVID 19 COVID-19 COVID-19 disease COVID-19 pathogenesis Critical disease drugs Effect FCgRT Gene Expression hematologic homolog humanized Immunity IMPROVE Infection Initiation codon knock-out Lung pathology mechanism modeling mouse model Multigene humanized mouse models offer pathology platform Precision preparation primary antibody protease random receptor receptors Research researcher Responder responders resulting SAR-CoV-2 SARS CoV SARS CoV-2 SARS CoV-2 infection selected strain suggested targets Th1 Th2 therapeutic efficacy TMPRSS2 TMRPSS2 transgene transgenic model unnatural Vaccine Variability [DOI] 10.1016/j.bcp.2021.114543 PMC 바로가기 [Article Type] Review
FDA-approved thiol-reacting drugs that potentially bind into the SARS-CoV-2 main protease, essential for viral replication바이러스 복제에 필수적인 SARS-CoV-2 주요 프로테아제에 잠재적으로 결합하는 FDA 승인 티올 반응 약물Article Published on 2021-06-012022-08-31 Journal: Journal of biomolecular structure & dynamics [Category] MERS, SARS, 치료제, [키워드] 3CL 3CL main protease 3CL pro absence ACE-2 acute respiratory syndrome alcoholism angiotensin Angiotensin-converting enzyme antimicrobial antiviral drug antiviral drugs Antiviral treatment binding Captopril cellular receptor Compound COVID-19 Cys145 disulfiram dose drug drugs Enzymes expression FDA-approved drug FDA-approved drugs high mortality host cell in silico Approach inactivation induce Infection inhibit Interaction Ligand mechanism MERS metabolite metabolites outbreak pandemic processing protease Proteins reaction replicase polyprotein replicase polyproteins required respiratory responsible Safe SARS-CoV-2 SARS-COV-2 infection Severe acute respiratory syndrome shown Side effects susceptible symptomatic tested the patient the SARS-CoV-2 therapy thiol thiol-reacting drugs. thought translated Vaccine Viral viral replication [DOI] 10.1080/07391102.2020.1764393 PMC 바로가기 [Article Type] Article
Time-dependent viral interference between influenza virus and coronavirus in the infection of differentiated porcine airway epithelial cellsResearch Article Published on 2021-05-252022-10-28 Journal: Virulence [Category] Coronavirus, [키워드] aggravate Air-liquid interface air-liquid interface culture airway epithelial cell aminopeptidase N applied caused cellular cellular receptor circulating Clinical signs Co-infection Complete inhibition coronavirus Coronavirus infection Coronavirus replication Culture detectable determinant epithelial cell H3N2 Human implication Infection Influenza virus innate immune response intervals molecular mechanism porcine aminopeptidase N porcine respiratory coronavirus porcine respiratory disease complex receptor Replication Respiratory Coronavirus responsible SARS-CoV-2 Seasonal influenza SIV super-infection swine influenza virus the timing time intervals tracheal upregulation viral interference virus virus infection viruses [DOI] 10.1080/21505594.2021.1911148 PMC 바로가기 [Article Type] Research Article