Ex vivo – Page 5 – Communicable Diseases Research

Angiopathic activity of LRG1 is induced by the IL-6/STAT3 pathway

Research

Published on 2022-03-222022-10-05
Journal: Scientific Reports [Category] 바이오마커,

Recombinant human interleukin-7 reverses T cell exhaustion ex vivo in critically ill COVID-19 patients

Research

Published on 2022-03-052022-10-28
Journal: Annals of Intensive Care [Category] COVID-19,

[키워드] administration Admission alteration alterations approaches Bacterial sepsis Blood Cell cell proliferation characteristic clinical setting collected COVID-19 COVID-19 patient COVID-19 patients Critically ill Critically ill patient CTLA-4 described evaluated Ex vivo Exhaustion expression feature flow cytometry hallmark Hospitalized ICU ICU admission ICU patient IFN-γ immune immunostimulation inhibited intensive care interleukin-7 lymphopenic marker mononuclear cell over-expression pathophysiology Pathways Patient PD-L1 peripheral proliferation recombinant Result reversed SARS-CoV-2 Sepsis severe coronavirus disease severe COVID-19 severe COVID-19 patient significantly supplementary material T cell T cell activation T cell proliferation T lymphocyte T lymphocyte response T lymphocytes therapeutic therapeutic strategy therapy transcriptomic profile treat
[DOI] 10.1186/s13613-022-00982-1 PMC 바로가기 [Article Type] Research

Early reduction of SARS-CoV-2-replication in bronchial epithelium by kinin B 2 receptor antagonism

키닌 B 2 수용체 길항작용에 의한 기관지 상피의 SARS-CoV-2 복제의 조기 감소

Original Article

Published on 2022-03-052022-09-10
Journal: Journal of Molecular Medicine (Berlin, Germany) [Category] COVID19(2023년), SARS, 유전자 메커니즘,

[키워드] ACE2 ACE2 receptor airway airway epithelial cell airway epithelial cells Airway inflammation Analysis analyzed antagonism antagonist antagonists approved assays B₂R-antagonist Bronchial epithelium cellular clinical study complex controls COVID-19 Critical cytotoxicity early-stage COVID-19 entry receptor epithelial epithelial cells Evidence Ex vivo expression of SARS-CoV-2 Future G protein Host Icatibant immune regulation in vitro in vivo in vivo model induction Infection information inhibiting inhibiting viral replication Interference Kallikrein Kinin Kinin-kallikrein-system mechanism murine nasal nasal mucosa negative nose pathway performed Plaque assay plaque assays PROTECT protection provide receptor receptor signaling reduce reduced reduction Replication RT-qPCR SARS-CoV-2 SARS-CoV-2 entry receptor SARS-CoV-2 replication SARS-CoV-2 RNA SARS-CoV-2-positive patients Signaling Spread study participant Study participants supplementary material symptomatic Transcriptome transcriptomes translational Transport treated Treatment upregulation Vero-E6 cells Viral viral replication were infected
[DOI] 10.1007/s00109-022-02182-7 PMC 바로가기 [Article Type] Original Article

Prognosis and antibody profiles in survivors of critical illness from COVID-19: a prospective multicentre cohort study

COVID-19로 인한 중병 생존자의 예후 및 항체 프로필: 전향적 다기관 코호트 연구

Multicenter Study

Published on 2022-03-012022-09-11
Journal: British journal of anaesthesia [Category] MERS, SARS, 진단, 치료기술,

[키워드] antibody Antigen carbon monoxide Characteristics Chest computed tomography China clinical Coagulation cognitive Cognitive function cohort study Computed tomography Control COVID-19 Critical Critical illness declined detectable diffusing diffusing capacity enrolled Ex vivo Follow-up function hospital IgG Immune cell immune cells Immune status Immunity Immunoglobulin impairment infected with COVID-19 inter-quartile range kidney life liver lung Lung function lung volumes lymphocyte Lymphocyte count majority mechanical ventilation Mental multicentre Multiple Neutralising Antibodies neutralising antibody Other outcome parameter participated Patient peptide physical predicted profile prospective cohort study remained required Residual volume SARS-CoV-2 significantly subtype survivor Survivors titre titres Trial Volume were assessed
[DOI] 10.1016/j.bja.2021.11.024 PMC 바로가기 [Article Type] Multicenter Study

Evolving Characteristics of Heart Transplantation Donors and Recipients: JACC Focus Seminar

Review article

Published on 2022-03-012022-10-05
Journal: Journal of the American College of Cardiology [Category] 비임상,

[키워드] allograft dysfunction Community death Donor donors Ex vivo expand focus heart heart failure hepatitis C virus include management Older age organ phenotype Prevent recent recipient Seminar transplantation ventricular dysfunction
[DOI] 10.1016/j.jacc.2021.11.064 [Article Type] Review article

Exploration of the ocular surface infection by SARS-CoV-2 and implications for corneal donation: An ex vivo study

SARS-COV-2에 의한 안구 표면 감염의 탐색 및 각막 기증에 대한 시사점 : 생체 외 연구

Research Article

Published on 2022-03-012022-09-10
Journal: PLoS Medicine [Category] MERS, SARS, 치료기술,

[키워드] 24 hour 24 hours ACE-2 acute respiratory syndrome aims Algorithms angiotensin angiotensin-converting enzyme 2 bulbar conjunctiva cathepsin cellular entry Conjunctiva Contamination contraindication contraindications Cornea Corneal Corneal Graft Corneal transplantation coronavirus coronavirus 2 coronavirus disease Coronavirus disease 2019 COVID-19 COVID-19 crisis Ct value Culture culture media decrease diagnosed diagnosed with COVID-19 Donor Donor selection donors enzyme epithelial cell epithelial cells epithelium evaluate Evidence Ex vivo exacerbated exploration expresse expressed expression expression of SARS-CoV-2 expression pattern female genetic material healthy donor immunohistochemistry implication IMPROVE increase in Infection infectious dose inoculation involved lack limbus limited sample size mandatory media median moderate molecular mRNA level nasopharyngeal Nasopharyngeal swab nasopharyngeal swabs Not contaminated Ocular surface organ organ culture pandemic pathway Patient PCR positive Positive test Postinfection promote promoted Protein qPCR quantified quantitative PCR question RdRP receptor Replication researcher respiratory respond retrieved reverse transcription Reverse transcription quantitative PCR risk risk of transmission RNA RNA copy RNA sequence RT-qPCR SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 receptor SARS-CoV-2 receptors SARS-CoV-2 RNA SARS-CoV-2 viral SARS-CoV-2 virus Severe acute respiratory syndrome severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 significant increase Support TCID tested tissue tissue culture tissues TMPRSS2 Transmission utility Viral Viral detection Viral RNA was tested were infected worsened
[DOI] 10.1371/journal.pmed.1003922 PMC 바로가기 [Article Type] Research Article

Memory B Cells Induced by Sputnik V Vaccination Produce SARS-CoV-2 Neutralizing Antibodies Upon Ex Vivo Restimulation

스푸트니크 백신 접종으로 유도된 기억 B 세포는 생체 외 재자극 시 SARS-CoV-2 중화 항체를 생성합니다

Article

Published on 2022-02-242022-09-11
Journal: Frontiers in Immunology [Category] COVID19(2023년), MERS, SARS, 변종, 진단,

[키워드] antibody Antibody Response Antibody responses approach B cell B cell response B.1.351 Beta Cell Cohort complement correlated COVID-19 cross-neutralize dependent on Effect effective vaccine Effective vaccines Ex vivo first dose Health humoral Immunity in-depth analysis induced lentivirus majority MBC MBCs memory B memory B cells memory cell naïve neutralized performed plasma Plasma cell Produce produced recipient response responses robust SARS-CoV-2 SARS-COV-2 infection serological serum antibodies Sputnik V vaccine stimulated subject the vaccine vaccination Vaccine vaccinee variant of SARS-CoV-2 variants of SARS-CoV-2 Wuhan
[DOI] 10.3389/fimmu.2022.840707 PMC 바로가기 [Article Type] Article

The Human Nose Organoid Respiratory Virus Model: an Ex Vivo Human Challenge Model To Study Respiratory Syncytial Virus (RSV) and Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Pathogenesis and Evaluate Therapeutics

Research Article

Published on 2022-02-152022-10-29
Journal: mBio [Category] COVID-19, MERS,

[키워드] acute respiratory syndrome Administered Air-liquid interface air-liquid interface (ALI) culture airway Airway organoids airways ALI cultures basolateral compartment challenge cilia ciliary ciliated cell circulation coronavirus coronavirus 2 Culture Cytokines Efficacy epithelium evaluated event Ex vivo feasibility generate Human human airway human respiratory virus human respiratory viruses immunoprophylaxis immunotherapies Infants Infection innate immune responses invasive lung organoid Model Mucus noninvasive technique nose nose organoids occurred Organoid organoids palivizumab Pathogenesis Patient predict Prevent prevented respiratory respiratory syncytial virus respiratory syncytial virus (RSV) respiratory viral respiratory virus RSV SARS-CoV-2 SARS-COV-2 infection severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Therapeutics unmet need viral infection viral shedding
[DOI] 10.1128/mbio.03511-21 PMC 바로가기 [Article Type] Research Article

Immune responses following the first dose of the Sputnik V (Gam-COVID-Vac)

Sputnik V의 첫 번째 용량 (Gam-Covid-Vac) 이후의 면역 반응

Comparative Study

Published on 2022-02-022022-09-01
Journal: Scientific Reports [Category] COVID19(2023년), MERS, SARS, 바이오마커, 진단,

[키워드] ACE2 receptor Adaptive immunity administration antibodies antibody Antimicrobial responses AZD1222 B.1.1.7 B.1.351 B.1.617.2 CD107a Cell ELISPOT ELISpot responses Ex vivo expressing first dose Frequency Gam-COVID-Vac IFNγ immunogenic immunogenicity immunology in some Infection Infectious diseases investigated Lymphocytes memory B memory B cell Microbiology Molecular medicine naïve individuals natural infection Receptor binding domain response responses second dose seroconversion rate seroconverted significantly higher significantly lower single dose T cell T cells titre Vaccine Vaccines
[DOI] 10.1038/s41598-022-05788-6 PMC 바로가기 [Article Type] Comparative Study

SARS-CoV-2 Omicron variant replication in human bronchus and lung ex vivo

Research

Published on 2022-02-012022-10-05
Journal: Nature [Category] COVID19(2023년), SARS, 변종, 진단,

[키워드] B.1.1.529 B.1.1.7 B.1.351 B.1.617.2 Beta cathepsin Cell cellular tropism Culture D614G Delta dependent on determinant epidemiological epidemiological studies evade evaluated Ex vivo exceptional explain faster global public health Human human bronchus human lung immune evasion Immunity Infection intrinsic less lung lung parenchyma Lungs multifactorial natural infection omicron other variant other variants reduced replicate Replication reported SARS-CoV-2 SARS-CoV-2 variant severity tested TMPRSS2 Transmissibility variant variant of SARS-CoV-2 variants of concern Virological wild type wild-type virus
[DOI] 10.1038/s41586-022-04479-6 [Article Type] Research