Identifying SARS-CoV-2 antiviral compounds by screening for small molecule inhibitors of nsp14/nsp10 exoribonucleaseBiochemical Techniques & Resources Published on 2021-07-162022-10-28 Journal: Biochemical Journal [Category] COVID-19, Fulltext, SARS, [키워드] Antiviral compound Antiviral treatment antiviral treatments antivirals approach approved biochemical assay cofactor Compound contributing to coronavirus COVID-19 effective enzymatic activity identify identifying in vitro inhibit inhibitor inhibitors non-structural protein nsp14 nsp14 exoribonuclease pandemic patulin proliferation purified Remdesivir replication of SARS-CoV-2 Research SARS-CoV-2 screened small molecule inhibitor Spread target therapeutic viral genome replication [DOI] 10.1042/BCJ20210198 PMC 바로가기 [Article Type] Biochemical Techniques & Resources
Coronavirus genomic nsp14-ExoN, structure, role, mechanism, and potential application as a drug target코로나바이러스 게놈 nsp14-ExoN, 구조, 역할, 메커니즘 및 약물 표적으로서의 잠재적 응용Review Published on 2021-07-012022-09-11 Journal: Journal of medical virology [Category] SARS, 유전자 메커니즘, 치료제, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 antiviral therapy antivirals coding Combination coronavirus coronavirus disease Coronavirus disease 2019 CoV COVID-19 CoVs domain drug target drugs Effect ExoN Exoribonuclease genome replication genomic global pandemic healthcare highlight human coronavirus human coronaviruses identify mechanism nonstructural protein 14 nsp14 nsp14-ExoN nucleoside nucleoside analog nucleoside analogs nucleotide nucleotides Proofreading Protein Replication required RNA RNA recombination SARS-CoV-2 severe acute respiratory syndrome Coronavirus target targets Transcription [DOI] 10.1002/jmv.27009 PMC 바로가기 [Article Type] Review
The Antiviral Effect of the Chemical Compounds Targeting DED/EDh Motifs of the Viral Proteins on Lymphocytic Choriomeningitis Virus and SARS-CoV-2Article Published on 2021-06-242022-10-28 Journal: Viruses [Category] COVID-19, SARS, [키워드] A549 antivirals arenavirus arenaviruses cell lines Cell toxicity cells Chemical Choriomeningitis Compound compounds Concentration coronavirus Coronaviruses DED/EDh motif drug target Effect exhibited exonuclease exonuclease (ExoN) motif include indicated inhibited LCMV Lymphocytic choriomeningitis Lymphocytic choriomeningitis virus (LCMV) MERS-CoV motif motifs nsp14 pathogenic viruses performed SARS-CoV SARS-CoV-2 SARS-COV-2 infection Simulation study targeting these compound treat Vero Viral protein Viral proteins virus was used [DOI] 10.3390/v13071220 PMC 바로가기 [Article Type] Article
Translational shutdown and evasion of the innate immune response by SARS-CoV-2 NSP14 proteinSARS-CoV-2 NSP14 단백질에 의한 타고난 면역 반응의 번역 차단 및 회피423 Published on 2021-06-152022-09-10 Journal: Proceedings of the National Academy of Sciences of [Category] MERS, SARS, 유전자 메커니즘, [키워드] active site active sites activities acute respiratory syndrome acute respiratory syndrome coronavirus addition Antiviral antiviral proteins antiviral response causative agent caused complex conserved coronavirus COVID-19 COVID-19 pandemic Critical demonstrated ENhance evade ExoN Exoribonuclease Health Health crisis Host host antiviral responses Host immune response host immune responses host protein human coronaviruses IFN-I IFN-I responses immune evasion immune responses Infection inhibit inhibition activity inhibitor inhibitory innate immune response Innate immunity interferon interferon-stimulated gene interferon-stimulated genes ISGs mechanism nsp14 overcome Pathogenesis pathogenesis of SARS-CoV-2 pathogenic virus pathogenic viruses Pathogens previous study Protein Proteins replicate required respiratory reveal SARS-CoV-2 severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 Significance subversion synthesis the SARS-CoV-2 translation translation inhibition translational type I interferon Viral viral pathogens virus replication viruses [DOI] 10.1073/pnas.2101161118 PMC 바로가기 [Article Type] 423
Crucial mutation in the exoribonuclease domain of nsp14 of PEDV leads to high genetic instability during viral replicationResearch Published on 2021-06-072022-11-01 Journal: Cell & Bioscience [Category] COVID-19, [키워드] active site acute infection acute respiratory syndrome Analysis Betacoronavirus Clinical signs clone contributing to coronavirus CoV Critical deficiency diarrhea died Disabled domain drugs epidemic diarrhea evaluate Exoribonuclease fecal functional Genetic genetics gnotobiotic in viral in vitro infectious clone inoculated investigated less MHV Middle East moderate murine hepatitis virus mutant mutants Mutation nonstructural protein 14 nsp14 nsp14-ExoN Pathogenesis PEDV pig plaque forming unit Porcine epidemic diarrhea virus predicted Proofreading replication fidelity reported rescued responsible Result reverse genetics Sanger sequencing SARS-CoV-2 sensitivity supplementary material syndrome TCID variant Vero cells viral genome viral replication Viral RNA virulent virus while Zinc [DOI] 10.1186/s13578-021-00598-1 PMC 바로가기 [Article Type] Research
A Biochemical Perspective of the Nonstructural Proteins (NSPs) and the Spike Protein of SARS CoV-2비구조 단백질(NSP)과 SARS CoV-2의 스파이크 단백질에 대한 생화학적 관점Review Published on 2021-06-012022-09-11 Journal: The protein journal [Category] SARS, 변종, [키워드] 501Y.V1 501Y.V2 ACE2 receptor aspartate caused change Chemistry D614G effort ENhance Enzymes global pandemic glycine Host increasingly Interaction N501Y nonstructural protein nonstructural proteins Nsp12 nsp13 nsp14 Nsp15 Nsp3 nsp5 pandemic position Proteases Proteins Receptor binding domain SARS CoV SARS CoV-2 Spike protein strain the Spike the spike protein variant Viral proteins virus [DOI] 10.1007/s10930-021-09967-8 PMC 바로가기 [Article Type] Review
A cell-based assay to discover inhibitors of SARS-CoV-2 RNA dependent RNA polymeraseSARS-CoV-2 RNA 의존성 RNA 중합효소의 억제제를 발견하기 위한 세포 기반 분석Article Published on 2021-06-012022-09-11 Journal: Antiviral Research [Category] SARS, 치료제, [키워드] addition Antiviral antiviral therapeutics antivirals biochemical assay Cell cells chemical process Compound conversion COVID-19 COVID-19 pandemic COVID-19 patients EC50 effective Efficacy entecavir evaluate evaluated examined Favipiravir forms High-throughput highest in vitro increase inhibitors inhibitors of SARS-CoV-2 molnupiravir nsp14 nucleotide Nucleotide analog inhibitor nucleotide prodrug nucleotide prodrugs occur Penciclovir polymerase provide RdRP Remdesivir ribavirin RNA RNA polymerase RNA-dependent RNA polymerase SARS-CoV-2 SARS-CoV-2 RdRp SARS-CoV-2 RdRp inhibitor Seven suggested tenofovir threshold Tolerance treat triphosphate utility Viral RNA viral RNA-dependent RNA polymerase [DOI] 10.1016/j.antiviral.2021.105078 PMC 바로가기 [Article Type] Article
Label-Free Screening of SARS-CoV-2 NSP14 Exonuclease Activity Using SAMDI Mass SpectrometryArticle Published on 2021-04-172022-10-29 Journal: SLAS discovery : advancing life sciences R & D [Category] COVID-19, MERS, SARS, [키워드] accelerate activity acute respiratory syndrome antiviral target Combination condition coronavirus COVID-19 COVID-19 pandemic Critical described Drug discovery effort enzyme activity exonuclease feature guanine implicated inhibitors Initiating Label-free lack mass mass spectrometry mechanism methodology methyltransferase monolayer nsp14 Nuclease Pathologies Protein responsible RNA robust SARS-CoV-2 SARS-CoV-2 replication Screening small molecule specificity Spectrometry virus was used [DOI] 10.1177/24725552211008854 PMC 바로가기 [Article Type] Article
Genetic conservation of SARS-CoV-2 RNA replication complex in globally circulating isolates and recently emerged variants from humans and minks suggests minimal pre-existing resistance to remdesivir전 세계적으로 순환하는 분리주와 최근에 출현한 인간과 밍크 변이체에서 SARS-CoV-2 RNA 복제 복합체의 유전적 보존은 렘데시비르에 대한 최소한의 기존 내성을 시사합니다Article Published on 2021-04-012022-09-11 Journal: Antiviral Research [Category] SARS, 변종, 유전자 메커니즘, [키워드] active site addition Amino acid amino acid substitution analyzed antiviral activity approved circulating complex coronavirus COVID-19 Critical Efficacy exhibit Frequency Genetic genetic diversity Genetic variation highest Human in vitro isolate isolates mechanism molecular target Mutation nsp10 Nsp12 nsp12 RdRp. nsp13 nsp14 Nsp7 NSP8 P323L polymerase RdRP RDV Remdesivir Replication resistance risk RNA RNA-dependent RNA polymerase SARS-CoV-2 SARS-CoV-2 RNA SARS-CoV-2 sequence SARS-CoV-2 variant SARS-CoV-2 variants selected sequence sequence variation sequences South Africa Spread stability Treatment treatment of COVID-19 United Kingdom variant variants virus [DOI] 10.1016/j.antiviral.2021.105033 PMC 바로가기 [Article Type] Article
Development of a High-Throughput Screening Assay to Identify Inhibitors of the SARS-CoV-2 Guanine-N7-Methyltransferase Using RapidFire Mass SpectrometryArticle Published on 2021-03-162022-10-29 Journal: SLAS discovery : advancing life sciences R & D [Category] COVID-19, SARS, [키워드] activity acute respiratory syndrome Analysis Antiviral conserved coronavirus Coronavirus-2 Coronaviruses COVID-19 COVID-19 infection develop development drug drugs Efficiency enzyme evade exonuclease food Formation Health help Host in viral inhibitor mass methyltransferase nsp14 Outbreaks Protein Replication responsible SARS-CoV SARS-CoV-2 Screening selective shown similarity specific treatment Spectrometry starting point the SARS-CoV-2 therapy translation viral genome Viral RNA [DOI] 10.1177/24725552211000652 PMC 바로가기 [Article Type] Article