A Treatment to Eliminate SARS-CoV-2 Replication in Human Airway Epithelial Cells Is Safe for Inhalation as an Aerosol in Healthy Human Subjects인간 기도 상피 세포에서 SARS-CoV-2 복제를 제거하는 치료법은 건강한 인간 대상에서 에어로졸로 흡입하기에 안전합니다Article Published on 2021-01-012022-08-31 Journal: Respiratory care [Category] SARS, 유전자 메커니즘, 치료법, 치료제, [키워드] Activation aerosol airway Airway inflammation airway pH. Cell cells clinical trial Concentration COVID-19 Defense determine Diseases endosomal trafficking expression had no healthy subjects Host host defense Human human airway epithelial humans impair in vivo Infection inhalation Inhaled inhibited isotonicity Lung function mechanisms multiplicity multiplicity of infection N protein no effect nucleocapsid osmolality Oxygenation Patient patient safety phase Placebo Plaque assay Prevent prevented protein levels raise raised Replication SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 viral replication subject subjects Treatment Trial USA-WA1/2020 viability Viral viral infection viral replication vital signs was determined [DOI] 10.4187/respcare.08425 PMC 바로가기 [Article Type] Article
Improved plaque assay for human coronaviruses 229E and OC43Biotechnology Published on 2020-12-212022-10-28 Journal: PeerJ [Category] COVID-19, [키워드] 229E Agarose antivirals approach Biosafety Level 3 BSL-2 BSL-3 can be used Cell cells comparable conditions Contact coronavirus Coronavirus infection COVID-19 COVID-19 pandemic effective facilitate faster growth HCoV 229E HCoV-229E Host immune response human coronavirus human coronaviruses 229E immunostaining Improved less Lung epithelial cells OC43 Plaque assay produced Research researcher SARS-CoV-2 sensitivity Stress temperature Tolerance turn [DOI] 10.7717/peerj.10639 PMC 바로가기 [Article Type] Biotechnology
Massive and rapid COVID-19 testing is feasible by extraction-free SARS-CoV-2 RT-PCRArticle Published on 2020-09-232022-10-04 Journal: Nature Communications [Category] SARS, 진단, 치료기술, 치료법, [키워드] acute respiratory syndrome buffer caused clinically coronavirus Coronavirus disease 2019 COVID-19 COVID-19 testing detect develop diagnosed Diagnosis diagnostic Effectiveness Efficiency evaluate feasible heat inactivation heat-inactivated infection control Laboratory techniques and procedures lysed massive medium Patient Patient care PCR-based testing Plaque assay polymerase chain protocol reverse transcription RNA extraction RT-PCR SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 RT-PCR Significant subject Transport viral infection Viral RNA [DOI] 10.1038/s41467-020-18611-5 PMC 바로가기 [Article Type] Article
Propagation, Inactivation, and Safety Testing of SARS-CoV-2Article Published on 2020-06-062022-10-28 Journal: Viruses [Category] COVID-19, SARS, [키워드] acute respiratory syndrome Biosafety Level 3 causes Cellulose Chinese coronavirus COVID-19 deaths described DNA effective facilitated focus forming assay inactivation infected cell Infection Infectious virus multiple cell line neutral buffered formalin new virus Novel coronavirus pandemic performed Plaque assay Propagation Protein quantification Research responsible resulting RNA Safe Safety SARS-CoV-2 Testing the disease Trizol Virology virus virus infectivity widespread worldwide pandemic Wuhan [DOI] 10.3390/v12060622 PMC 바로가기 [Article Type] Article
Pathogen reduction of SARS-CoV-2 virus in plasma and whole blood using riboflavin and UV lightResearch Article Published on 2020-05-292022-10-28 Journal: PLoS ONE [Category] COVID-19, MERS, SARS, [키워드] average Blood blood product categories clade coronavirus 2 COVID-19 Critically ill patient described dose Efficacy exposure to Human plasma Immunity infectious titer limit of detection Log reduction pathogen performed Plaque assay plasma question reduce reduced reducing reduction repeated respiratory Result Riboflavin SARS-CoV-2 SARS-CoV-2 virus serum System technology tested the mean transmitted Treatment ultraviolet light USA-WA1/2020 UV light VERO E6 cells Viral RNA viral titer was used Whole blood with COVID-19 [DOI] 10.1371/journal.pone.0233947 PMC 바로가기 [Article Type] Research Article
Divalent copper complexes as influenza A M2 inhibitorsComparative Study Published on 2017-11-012023-06-23 Journal: Antiviral Research [Category] 신종인플루엔자, [키워드] Electrophysiology Medicinal metals Plaque assay Proton transport Transfected oocytes Tridentate chelation. [DOI] 10.1016/j.antiviral.2017.10.009 [Article Type] Comparative Study
Bacillus subtilis and surfactin inhibit the transmissible gastroenteritis virus from entering the intestinal epithelial cellsResearch Articles Published on 2017-03-072022-10-28 Journal: Bioscience Reports [Category] Coronavirus, [키워드] addition aminopeptidase N Analysis Antiviral APN Apoptosis apoptotic cells B. subtilis Bacillus subtilis Bacillus subtilis OKB105 candidate Cell coronavirus declined develop dose-dependent manner EGFR ENhance Entry epidermal epithelial cell epithelial cell line experiment expression gastroenteritis growth factor host cell in vitro indicated Infection inhibit intestinal intestinal epithelial cell mechanisms Particle Pathogens performed Plaque assay Protein receptor receptors reduce reducing surfactin target TGEV Toll-like receptor up-regulating viral entry virus western blotting [DOI] 10.1042/BSR20170082 PMC 바로가기 [Article Type] Research Articles
Effective inhibition of MERS-CoV infection by resveratrolResearch Article Published on 2017-02-132022-10-28 Journal: BMC Infectious Diseases [Category] Coronavirus, [키워드] addition administration analyzed Antiviral antiviral activity antiviral medicine antiviral property Apoptosis approved can be used cause cellular Concentration coronavirus demonstrated down-regulated Effectiveness expression in vitro inhibited inhibition inhibitory MERS-CoV MERS-CoV infection MERS-CoV virus infection MERS-CoV-infected patients Middle East Middle East Respiratory Syndrome Virus morbidity and mortality MTT neutral red nucleocapsid performed Plaque assay Protein protein expression quantitative PCR reduce Replication respiratory Result resveratrol significantly survival survival rate treat Treatment Vaccine VERO E6 cells viral pathogen Viral RNA virus infection [DOI] 10.1186/s12879-017-2253-8 PMC 바로가기 [Article Type] Research Article
Optimization of a Quantitative Micro-neutralization AssayArticle Published on 2016-12-142023-06-23 Journal: Journal of visualized experiments : JoVE [Category] 신종인플루엔자, [키워드] antigenicity flatbed scanner Hemagglutination inhibition high-throughput imaging Infection Influenza Issue 118 Micro-neutralization Plaque assay virus-infected cell quantitation [DOI] 10.3791/54897 PMC 바로가기
Molecular Characterization of Polio from Environmental Samples: ISSP, The Israeli Sewage Surveillance Protocol환경 샘플에서 폴리오의 분자적 특성 분석: 이스라엘 하수 감시 프로토콜 (ISSP)book-chapter Published on 2016-01-012024-08-01 Journal: Methods in molecular biology (Clifton, N.J.) [Category] 폴리오, [키워드] Environmental surveillance Intratypic differentiation oral polio vaccine (OPV) Plaque assay Poliovirus qRT-PCR RNA purification RT-PCR Sequence analysis tissue culture Vaccine-derived poliovirus (VDPV) wild poliovirus [DOI] 10.1007/978-1-4939-3292-4_5 [Article Type] book-chapter