Identification of FDA approved drugs and nucleoside analogues as potential SARS-CoV-2 A1pp domain inhibitor: An in silico study잠재적인 SARS-CoV-2 A1pp 도메인 억제제로서 FDA 승인 약물 및 뉴클레오시드 유사체 식별: 실리코 연구Article Published on 2021-03-012022-09-11 Journal: Computers in biology and medicine [Category] SARS, 신약개발, 치료제, [키워드] A1pp domain adenosine adenosine diphosphate Analysis approved binding binding affinity binding free energy biological activity citicholine conserved coronavirus crystal structure demonstrated deviation docking scores domain exhibited FDA FDA approved drug FDA approved Drugs FIVE fluctuation free energy free energy landscape gyration Host human coronavirus hydrogen Hydrogen bond hydrophobic identification in silico infect inhibit inhibitor Interaction intestine macro Macro domain molecular molecular dynamics Molecular dynamics simulation Molecular mechanics nadide nucleoside nucleoside analogue Nucleoside analogues offer Pathogenesis performed phosphatase positive control promote Protein radius radius of gyration reported respiratory tract ribose RMSD RMSF root mean square deviation root mean square fluctuation SARS-CoV-2 solvent stable complex substrate binding site surface area Therapeutic molecule Therapeutics these compound these compounds These viruses these viruses viral infection [DOI] 10.1016/j.compbiomed.2020.104185 PMC 바로가기 [Article Type] Article
Evaluation of two automated low-cost RNA extraction protocols for SARS-CoV-2 detectionResearch Article Published on 2021-02-162022-10-28 Journal: PLoS ONE [Category] COVID-19, [키워드] automated average calculated collected described detect DNA Efficiency evaluated handling magnetic bead Nasopharyngeal swab positive positive control positive sample protocol reagent Result RNA extraction RNA extraction protocol SARS-CoV-2 SARS-CoV-2 detection significant difference tested [DOI] 10.1371/journal.pone.0246302 PMC 바로가기 [Article Type] Research Article
Chimeric synthetic reference standards enable cross-validation of positive and negative controls in SARS-CoV-2 molecular testsArticle Published on 2021-01-292022-10-29 Journal: Scientific Reports [Category] COVID-19, MERS, [키워드] Accuracy approach chimeric conditions Control controls current diagnostic Diagnostic markers diagnostic tests DNA evaluate feature Genetic genetic material in vitro molecular test negative control offer Organisms Patient positive positive control protocol provide real-time RT-PCR reference standard required respiratory RNA standards RT-PCR SARS-CoV-2 sensitivity specificity target region viral infection were assessed [DOI] 10.1038/s41598-021-81760-0 PMC 바로가기 [Article Type] Article
A cluster-control approach to a coronavirus disease 2019 (COVID-19) outbreak on a stroke ward with infection control considerations for dementia and vascular units치매 및 혈관 단위에 대한 감염 제어 고려 사항이 있는 뇌졸중 병동에서 코로나바이러스 질병 2019(COVID-19) 발생에 대한 클러스터 제어 접근 방식Original Article Published on 2021-01-112022-09-10 Journal: Infection Control and Hospital Epidemiology [Category] MERS, SARS, 치료기술, [키워드] 95% CI antibody approach Asymptomatic Chain Reaction Cluster Compliance Contamination control ward control wards coronavirus disease Coronavirus disease 2019 COVID-19 cycle threshold Dementia detectable environmental contamination evaluate Factors hand hygiene hygiene IgG antibody infection control negative control no difference Non-COVID-19 observation outbreak Outbreak investigation Outbreaks Patient patient behavior Personal protective equipment polymerase chain polymerase chain reaction positive positive control PPE PPE compliance presymptomatic presymptomatic transmission Prevalence Prevent proportion Randomly resulting reverse-transcriptase polymerase chain reaction RNA RT-PCR SARS-CoV-2 SARS-CoV-2 IgG antibody secondary cases selected Seroconversion staff stroke sustained symptomatic individuals tested Vascular were assessed [DOI] 10.1017/ice.2020.1437 PMC 바로가기 [Article Type] Original Article
Cellular Immunity in COVID-19 Convalescents with PCR-Confirmed Infection but with Undetectable SARS-CoV-2–Specific IgGResearch Published on 2021-01-012022-10-31 Journal: Emerging Infectious Diseases [Category] COVID-19, [키워드] acute respiratory syndrome analyzed antibody Antibody Response B cells blood donor cellular immunity Contact convalescent convalescent patient convalescent plasma coronavirus coronavirus disease COVID-19 detect Donor ELISA ELISPOT Frequency Germany IgG immune response Immunity infected patient Infection interferon-γ investigated negative controls onset of symptoms participant PCR-confirmed PCR-confirmed infection PCR-positive positive control respiratory infections SARS SARS-CoV-2 SARS-CoV-2 IgG SARS-COV-2 infection Seronegativity Severe acute respiratory syndrome severe acute respiratory syndrome coronavirus 2 study participant T cell T cells T-cell immunity the S1 protein undetectable viruses Volunteer without symptom zoonoses [DOI] 10.3201/eid2701.203772 PMC 바로가기 [Article Type] Research
The SARS-Cov-2 Proliferation Blocked by a Novel and Potent Main Protease Inhibitor via Computer-aided Drug DesignOriginal Article Published on 2021-01-012022-10-30 Journal: Iranian Journal of Pharmaceutical Research : IJPR [Category] COVID-19, [키워드] ADME ADMET Analysis approval Area binding affinity binding energy complex Compound computation coronavirus disease COVID-19 DCCM drug drug design effort In-vitro inhibit Ligand MD simulation MM-PBSA molecular docking Molecular dynamic novel outcome pandemic PCA positive control potent Prevalence protease protease inhibitors residue selected stability surface treat Virtual screening was used were used [DOI] 10.22037/ijpr.2021.114846.15061 PMC 바로가기 [Article Type] Original Article
Development of immunohistochemistry and in situ hybridisation for the detection of SARS-CoV and SARS-CoV-2 in formalin-fixed paraffin-embedded specimens포르말린 고정 파라핀 포매 표본에서 SARS-CoV 및 SARS-CoV-2 검출을 위한 면역조직화학 및 in situ hybridization 개발Article Published on 2020-12-142022-09-01 Journal: Scientific Reports [Category] MERS, SARS, 진단, 치료기술, 치료법, [키워드] addition against SARS-CoV animal animal models antibody Antigen antigen retrieval method applied causative agent caused coronavirus coronavirus pathogenesis COVID-19 demonstrated detect determine development dissemination evaluated expressing facilitate FFPE flexibility formalin formalin-fixed paraffin-embedded IHC immunohistochemistry infected cell infected cells Live virus MERS Microscopy non-infected cell non-infected cells nucleoprotein paraffin positive control probe protocol public health Research RNA SARS-CoV SARS-CoV spike protein SARS-CoV-2 SARS-CoV-2 viral specimen spike gene Spike protein tested therapeutic Therapeutics tissues utility Vaccine Viral virus virus detection [DOI] 10.1038/s41598-020-78949-0 PMC 바로가기 [Article Type] Article
Epidemiological and viral features of a cohort of SARS-CoV-2 symptomatic and asymptomatic individuals in an area of the Colombian Caribbean콜롬비아 카리브해 지역의 SARS-CoV-2 증상 및 무증상 개인 코호트의 역학 및 바이러스 특징Short Report Published on 2020-12-072022-09-10 Journal: Annals of Clinical Microbiology and Antimicrobials [Category] MERS, SARS, 치료기술, 치료제, [키워드] 2019-nCoV assay acute respiratory syndrome acute respiratory syndrome coronavirus age approach arthralgia Asymptomatic asymptomatic individual asymptomatic infections asymptomatic patient asymptomatic patients average average age caused Chain Reaction clinical sample co-morbidities Cohort Communicable disease control Contact Córdoba coronavirus coronavirus 2 Coronavirus infections cough country COVID-19 Critical deaths Department detect develop Dilution Environment and public health feature Fever group Health hospital implication individual individuals Infection Laboratory lack lysis buffer molecular morbidities MOST multiplex nasopharyngeal sample Nasopharyngeal samples objective Occurrence odynophagia pandemic pandemic disease patients required hospitalization PCR assay polymerase chain polymerase chain reaction positive positive control Pre symptomatic disease presymptomatic proportion Public Public health intervention Public health interventions quantification real-time PCR assay reduced regions reported respiratory Result reverse transcription reverse transcription real-time polymerase chain reaction RNA RNA copies RNA copy RT-qPCR SARS-CoV-2 SARS-CoV-2 infected individual SARS-CoV-2 infected individuals SARS-COV-2 infection severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 significantly higher socio-demographic South America standard curve symptomatic symptomatic and asymptomatic symptomatic patient Symptomatic patients Symptoms target gene Target genes tested the patient Transmission Viral viral load quantification was done were used [DOI] 10.1186/s12941-020-00397-5 PMC 바로가기 [Article Type] Short Report
Clean Grinding Technique: A Facile Synthesis and In Silico Antiviral Activity of Hydrazones, Pyrazoles, and Pyrazines Bearing Thiazole Moiety against SARS-CoV-2 Main Protease (M pro )Article Published on 2020-10-062022-10-30 Journal: Molecules [Category] COVID-19, MERS, SARS, [키워드] activity anti-viral activity average Bearing binding affinity Compound compounds condensation cyclo-condensation derivatives docking Evidence Grinding hydrazide hydrazone lifecycle M pro MDs moiety molecular docking Molecular dynamics simulation nelfinavir positive control SARS-CoV-2 SARS-CoV-2 Mpro SARS-CoV-2 main protease SARS-CoV-2 Mpro Structure synthesis virus was tested [DOI] 10.3390/molecules25194565 PMC 바로가기 [Article Type] Article
High-coverage SARS-CoV-2 genome sequences acquired by target capture sequencing표적 포획 시퀀싱으로 획득한 고범위 SARS-CoV-2 게놈 서열Article Published on 2020-10-012022-09-11 Journal: Journal of medical virology [Category] SARS, 변종, 치료기술, 치료제, [키워드] Biotin can be used capture capture sequencing CDC Cell culture Concentration coronavirus Coronavirus replication cycle threshold demonstrated diagnosed with COVID-19 expression Gene Expression Genetic genetic networks genetic variability genome sequence Health heterozygosity knowledge Map Mutation Nasopharyngeal swab nasopharyngeal swab sample negative controls Patient patients without COVID-19 Phylogenetic relationship positive control probe SARS Coronavirus SARS-CoV-2 SARS-CoV-2 genome sequence SARS-CoV-2 sequence SARS-CoV-2 sequences SARS-CoV-2 viral selected sequence single-stranded DNA subtype swab sample throat swab Viral Viral particles virus detection virus strain [DOI] 10.1002/jmv.26116 PMC 바로가기 [Article Type] Article