Identification, mapping and relative quantitation of SARS-CoV-2 Spike glycopeptides by Mass-Retention Time FingerprintingArticle Published on 2021-08-032022-10-31 Journal: Communications Biology [Category] COVID-19, [키워드] Analysis can be used Chalk Combination database Efficiency glycan glycobiology Greenland HPLC identification identify knowledge Laboratory LC-MS less mapping mass spectrometry MS3 peptide Protein protein digest quantitation resolved resolving power SARS-CoV-2 spike Structure time while [DOI] 10.1038/s42003-021-02455-w PMC 바로가기 [Article Type] Article
Lack of active SARS-CoV-2 virus in a subset of PCR-positive COVID-19 congregate care patientsPCR 양성 COVID-19 집단 치료 환자의 하위 집합에서 활성 SARS-CoV-2 바이러스 부족Article Published on 2021-08-012022-09-11 Journal: Journal of Clinical Virology [Category] SARS, 치료기술, [키워드] Accuracy antibody Antibody titer Care collected correlated COVID-19 Culture cultures detect diagnosis of COVID-19 disease genomic incidence interferon interferon signaling log NAAT Nasopharyngeal swab nasopharyngeal swab sample Nasopharyngeal swab samples non-infectious not affect nucleic acid nucleic acid amplification nucleic acid amplification tests occurred pathway Patient patients PCR-positive plaque Plaque assay plaque-forming unit positive qPCR quantitation RNA fragment RT-qPCR SARS-CoV-2 SARS-CoV-2 RNA SARS-CoV-2 virus signaling pathway Stage Standard of care Stat1 Sub-genomic RNA subset symptom onset tested Vero Vero E6 Vero E6 cell VERO E6 cells Vero STAT1(−/−). Viral RNA viral RNA load virus [DOI] 10.1016/j.jcv.2021.104879 PMC 바로가기 [Article Type] Article
Development and validation of a sensitive, fast and simple LC-MS / MS method for the quantitation of favipiravir in human serum인간 혈청에서 favipiravir의 정량을 위한 민감하고 빠르고 간단한 LC-MS/MS 방법의 개발 및 검증Article Published on 2021-06-302022-09-11 Journal: Journal of chromatography. B, Analytical technolog [Category] 치료제, [키워드] applicability applied biological samples clinical trial Concentration coronavirus disease Coronavirus disease 2019 correlation coefficient COVID-19 COVID-19 patient develop development drug level electrospray ionization extraction Favipiravir Formic acid human serum inhibitor LC-MS less linear mass spectrometry methanol patients with COVID-19 performed Phenomenex pilot study plasma samples polymerase positive protein precipitation quantitation receiving reported robust simple tandem Tandem mass spectrometry Treatment validation validation study Viral RNA was performed was used water [DOI] 10.1016/j.jchromb.2021.122768 PMC 바로가기 [Article Type] Article
In vitro Characterization of Fitness and Convalescent Antibody Neutralization of SARS-CoV-2 Cluster 5 Variant Emerging in Mink at Danish FarmsMicrobiology Published on 2021-06-252022-10-31 Journal: Frontiers in Microbiology [Category] COVID-19, [키워드] Active surveillance acute respiratory syndrome addition alteration Amino acid amino acid change amino acid residue amino acid substitution animal hosts antigenicity cell line characterization characterized consequence convalescent coronavirus country COVID-19 COVID-19 samples cynomolgus macaques Deletion demonstrated Denmark Europe evaluated Evolution farm ferrets fitness genomic hamsters humans in vitro include microneutralization mink neutralization North America Potential proportion quantitation reduced reported rhesus macaques SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 variant Spread Substitution susceptible Temporal the spike protein variant VERO-E6 Viral RNA virulence virus Virus neutralization Virus titration Wuhan-Hu-1 Y453F [DOI] 10.3389/fmicb.2021.698944 PMC 바로가기 [Article Type] Microbiology
High-throughput quantitation of SARS-CoV-2 antibodies in a single-dilution homogeneous assay단일-경막 균질 분석에서 SARS-CoV-2 항체의 고 처리량 정량Article Published on 2021-06-102022-09-01 Journal: Scientific Reports [Category] MERS, SARS, 진단, 치료기술, [키워드] analyzed antibody Antibody titer Antibody titers approach asymptomatic infections comparable convalescence COVID-19 dilution series Efficacy ELISA ELISA testing enzyme enzyme-linked immunosorbent enzyme-linked immunosorbent assay faster homogeneous Infection Infectious-disease diagnostics limitation overcome pandemic positive sample quantitation Quantitative quantity regimens representing respiratory Respiratory disease SARS-CoV-2 SARS-CoV-2 antibody screened Sensitivity and specificity specific antibodies Spread stability vaccination Vaccines viral infection virus [DOI] 10.1038/s41598-021-91300-5 PMC 바로가기 [Article Type] Article
Identify. Quantify. Predict. Why Immunologists Should Widely Use Molecular Imaging for Coronavirus Disease 2019식별하다. 정량화. 예측하다. 면역학자들이 코로나바이러스 질병 2019에 대해 분자 영상을 널리 사용해야 하는 이유Review Published on 2021-05-132022-09-12 Journal: Frontiers in Immunology [Category] SARS, 진단, [키워드] ACE-2-receptor acute infection antibody Biomarker cellular and molecular Course COVID-19 COX-2 CXCR4 Cytokine storm diagnostic procedure disease disease severity Effect Glucose highlighting imaging immune immunologist Infection knowledge mAbs management metabolism molecular molecular imaging monoclonal antibody oncology organs P2X7 (purino) receptor Pathomechanism pathophysiological peptide Perspective pet PET/CT probes prognostic quantitation Quantitative radiolabeled Research response SARS-CoV-2 target targeted therapy targets translate treatment approach [DOI] 10.3389/fimmu.2021.568959 PMC 바로가기 [Article Type] Review
A rigorous evaluation of optimal peptide targets for MS-based clinical diagnostics of Coronavirus Disease 2019 (COVID-19)Research Published on 2021-05-102022-11-01 Journal: Clinical Proteomics [Category] COVID-19, [키워드] amplifying Analysis analyzed assays automated bioinformatics Care Cell culture clinical samples collected coronavirus coronavirus 2 COVID-19 COVID-19 infection status Cutoff dataset detect detection Diagnosis diagnosis of SARS-CoV-2 diagnostic diagnostics Evidence global pandemic highest human host in vitro Infection Limits of detection mass spectrometry membrane protein molecular nucleocapsid pandemic Patient peptide Peptide-detection Proteins proteomics quantitation respiratory Result SARS-CoV-2 seeking sequence specificity Spread sudden supplementary material target the SARS-CoV-2 virus upper respiratory tract viral peptide Viral proteome Viral RNA Visualization with COVID-19 Workflows [DOI] 10.1186/s12014-021-09321-1 PMC 바로가기 [Article Type] Research
Age, Disease Severity and Ethnicity Influence Humoral Responses in a Multi-Ethnic COVID-19 Cohort다민족 COVID-19 코호트에서 연령, 질병 심각도 및 인종이 체액 반응에 영향을 미침Article Published on 2021-04-282022-09-01 Journal: Viruses [Category] MERS, SARS, 진단, 치료법, [키워드] affected age Antibody titer Antibody titers breadth C-terminal Case-control Cohort cohorts Complete Control Coverage COVID-19 COVID-19 pandemic disease severity domain Efficacy epitope epitope coverage Ethnicity expected Factors Genetic gens globe humoral humoral immune response Humoral response immunoassay in some increasing age independent individual individuals influence intrinsically Localization magnitude Microarray Mild motifs natural infection nucleocapsid protein Older Patient Population Protein protein microarray provided quantitation Quantitative quantitative antibody binding Quantitative data regions reported response SARS-CoV-2 antibodies SARS-CoV-2 nucleocapsid protein SARS-CoV-2 viral Sensitivity and specificity Serological assay severe disease significant difference significant differences significant increase significantly significantly higher strength tested the microarray the viral nucleocapsid Vaccine vaccine efficacy Viral viral nucleocapsid protein [DOI] 10.3390/v13050786 PMC 바로가기 [Article Type] Article
FIB-SEM as a Volume Electron Microscopy Approach to Study Cellular Architectures in SARS-CoV-2 and Other Viral Infections: A Practical Primer for a VirologistSARS-CoV-2 및 기타 바이러스 감염에서 세포 구조를 연구하기 위한 부피 전자 현미경 접근 방식으로서의 FIB-SEM: 바이러스 전문의를 위한 실용적인 입문서Review Published on 2021-04-022022-09-01 Journal: Viruses [Category] MERS, SARS, 진단, [키워드] architecture automated beam Cell cellular electron electron microscopy experiment FIB-SEM in vitro study increasingly interactions ion beam iterative less Microscopy Other plasma membrane positive primer quantitation reveal room temperature SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 virus SARS-CoV-2 viruses scanning electron microscopy Segmentation specimen temperature ultrastructure unique vEM Viral virological synapse Virologist viruses Visualization Volume volumeEM [DOI] 10.3390/v13040611 PMC 바로가기 [Article Type] Review
Quantitative Measurement of Anti-SARS-CoV-2 Antibodies: Analytical and Clinical EvaluationAnti-SARS-CoV-2 항체의 정량적 측정: 분석 및 임상 평가Article Published on 2021-03-192022-09-11 Journal: Journal of Clinical Microbiology [Category] SARS, 진단, 치료기술, 치료제, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 analytical sensitivity analyzed Anti-SARS-CoV-2 S antibody Antibody Response Antibody testing Antibody titer causative agent claimed clinical clinical evaluation clinical sensitivity coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 cross-reactivity detect determine diagnose Elecsy evaluated exhibited facilitate help highest immunoassays Infection Infectious disease interindividual variation limit linearity measure measurement Negative predictive value NPV Patient PCR positivity PCR-positive patient Positive predictive value PPV Predictive value Quality control quantitation Quantitative respiratory Roche Roche Diagnostics S protein SARS-CoV-2 sensitivity serologic assay serologic testing serology severe acute respiratory syndrome Coronavirus specificity specimen target the antibody response the S protein U/mL Vaccines Variation Virology while [DOI] 10.1128/JCM.03149-20 PMC 바로가기 [Article Type] Article