Beneficial Effects of Mineralocorticoid Receptor Pathway Blockade against Endothelial Inflammation Induced by SARS-CoV-2 Spike ProteinArticle Published on 2021-06-032022-10-28 Journal: Biomedicines [Category] COVID-19, [키워드] abnormal coagulation Activation antagonist blockade complications COVID-19 patients downstream dysfunction Effect ELISA endothelial Endothelial cell endothelial cells galectin-3 induced Inflammation Inflammatory Inflammatory response inhibitor interferon-γ interleukin-6 involved marker Mineralocorticoid mineralocorticoid receptor monocyte chemoattractant protein-1 pathogenesis of COVID-19 pathway prevented Protein receptor recombinant S protein reduce reversed S protein SARS-CoV-2 SARS-COV-2 infection SARS-CoV2 secretion Signaling spike supernatant thrombosis treated were assessed western blotting [DOI] 10.3390/biomedicines9060639 PMC 바로가기 [Article Type] Article
Human cardiosphere-derived stromal cells exposed to SARS-CoV-2 evolve into hyper-inflammatory/pro-fibrotic phenotype and produce infective viral particles depending on the levels of ACE2 receptor expressionSARS-CoV-2에 노출된 인간 심장권 유래 기질 세포는 ACE2 수용체 발현 수준에 따라 과염증/친섬유증 표현형으로 진화하고 감염성 바이러스 입자를 생성합니다Article Published on 2021-05-252022-09-11 Journal: Cardiovascular Research [Category] SARS, 치료기술, [키워드] ACE2 ACE2 receptor analysed angiotensin Angiotensin-converting enzyme angiotensin-converting enzyme-2 cardiac cardiac complication Cardiac complications cardiac injury Cardiac stromal cells caused Cell cellular Complication conditions COVID-19 demonstrated detect determine electron microscopy explain exposure to expression fibrosis High-resolution Human in vitro infected patients Infection infective Inflammation Injury Innate immunity involved Microscopy Myocardial Myocardium pathway PCR performed phenotype proteomics regulated reported RT-qPCR assay RT-qPCR assays SARS-CoV-2 SARS-COV-2 infection sensitivity Severe case severe cases severe respiratory syndrome stromal cell stromal cells supernatant susceptible these cell These cells transcriptomics undergo viral particle Viral particles virus [DOI] 10.1093/cvr/cvab082 PMC 바로가기 [Article Type] Article
The detection and stability of the SARS-CoV-2 RNA biomarkers in wastewater influent in Helsinki, Finland핀란드 헬싱키의 폐수 유입수에서 SARS-CoV-2 RNA 바이오마커의 검출 및 안정성Article Published on 2021-05-202022-09-11 Journal: The Science of the total environment [Category] MERS, SARS, 바이오마커, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 addition Analysis analyzed Betacoronavirus Biomarker collected conditions copy numbers coronavirus COVID-19 disease decay Decay characteristics fraction Frequency Helsinki IMPROVE influent linear lockdown lockdowns many cases MONITOR Municipal sewage New New coronavirus Norovirus Particulate matter persistence Prevalence quantified quantitative PCR remained respiratory RNA RNA copy number RNA copy numbers RT-qPCR SARS-CoV-2 SARS-CoV-2 RNA SARS-CoV-2 virus severe acute respiratory syndrome Coronavirus stability statistically significant storage storage condition storage conditions supernatant Surveillance temperature the SARS-CoV-2 the SARS-CoV-2 virus Treatment Ultrafiltration was tested wastewater water [DOI] 10.1016/j.scitotenv.2021.145274 PMC 바로가기 [Article Type] Article
Attenuation of porcine deltacoronavirus disease severity by porcine reproductive and respiratory syndrome virus coinfection in a weaning pig modelResearch Article Published on 2021-04-022022-10-28 Journal: Virulence [Category] Coronavirus, [키워드] attenuation average blocked cause caused cells cellular entry Coinfection cytokine Cytokines diarrhea disease manifestation disease severity DPI expressed fecal groups IHC IL-1 IL-6 immunosuppressive indicated inhibit inoculated Interaction intestine intestines intranasally lesion less macrophages microenvironment Neonatal PAM pathogen Pathogenesis pathogenicity Porcine deltacoronavirus porcine reproductive and respiratory syndrome virus Proinflammatory cytokine proinflammatory cytokines proliferation Regulation Replication reported reproductive risk secreted severity supernatant syndrome TNF-α virus weaning zoonotic [DOI] 10.1080/21505594.2021.1908742 PMC 바로가기 [Article Type] Research Article
Efficient detection of SARS-CoV-2 RNA in the solid fraction of wastewater폐수의 고체 분획에서 SARS-CoV-2 RNA의 효율적인 검출Article Published on 2021-04-012022-09-11 Journal: The Science of the total environment [Category] SARS, 치료기술, [키워드] Adsorption assays collected Concentration coronavirus disease Coronavirus disease 2019 correlated correlation COVID-19 COVID-19 case Data collection demonstrated effective efficient Environmental surveillance expected feces fraction glycol increasingly infected individuals investigated Japan manhole membrane Mild pandemic plant Polyethylene glycol prevalent quantitative PCR recorded reported RNA RNA concentration RNA concentrations SARS-CoV-2 SARS-CoV-2 RNA sewage significantly Spearman supernatant Surveillance the SARS-CoV-2 Treatment Ultrafiltration virus wastewater [DOI] 10.1016/j.scitotenv.2020.144587 PMC 바로가기 [Article Type] Article
Expression and Purification of a PEDV-Neutralizing Antibody and Its Functional VerificationArticle Published on 2021-03-122022-10-28 Journal: Viruses [Category] Coronavirus, [키워드] antibodies antibody demonstrated epidemic diarrhea eukaryotic expression vector experiment expressed Genes HEK293 cell Immunity Infection inhibited monoclonal antibody morbidity and mortality neutralized neutralizing antibody Newborn oral administration pathogenic virus PEDV problem produced S protein Side effects Strains supernatant Vaccines Variation verification virus [DOI] 10.3390/v13030472 PMC 바로가기 [Article Type] Article
A patient infected with SARS-CoV-2 over 100 days100일 이상 SARS-CoV-2에 감염된 환자Case Reports Published on 2021-02-182022-09-11 Journal: QJM: An International Journal of Medicine [Category] MERS, 진단, 치료기술, [키워드] acute infection be positive Blood Cell Cell culture cells Chain Reaction clinic visit COVID-19 infection Ct value Ct values Culture culture supernatant Cytopathic effect cytopathic effects diagnosed FIVE Health Illumina infected with COVID-19 infected with SARS-CoV-2 Infection inoculated Isolation Japanese nasopharyngeal specimen Nasopharyngeal swab Neutralizing antibodies neutralizing antibody outpatient clinic passage Patient positive qRT-PCR Quantitative respiratory respiratory tract infection reverse transcriptase Saliva SARS-CoV-2 SARS-CoV-2 RNA sequence Sequencing specimen sputum Stool supernatant Symptom symptoms of COVID-19 tested the patient TMPRSS2 cells Transmission upper respiratory tract upper respiratory tract infection Urine Viral Viral RNA [DOI] 10.1093/qjmed/hcaa296 PMC 바로가기 [Article Type] Case Reports
Fluoxetine Can Inhibit SARS-CoV-2 In VitroArticle Published on 2021-02-092022-10-29 Journal: Microorganisms [Category] COVID-19, [키워드] acute infection acute respiratory syndrome added Administered affecting Antiviral antiviral activity Can controls coronavirus coronavirus disease Cytopathic effect Diseases drug explain Fluoxetine Health in vitro in vivo inhibit inhibit SARS-CoV-2 inhibitor inhibitors of SARS-CoV-2 investigated limit of detection mechanism outbreak pandemic Particle Post-infection Replication RNA viruses SARS-CoV-2 SARS-CoV-2 infections selective Serotonin supernatant the disease treat Treatment strategies VERO E6 cells virus [DOI] 10.3390/microorganisms9020339 PMC 바로가기 [Article Type] Article
Human Dental Pulp Stem Cells Modulate Cytokine Production in vitro by Peripheral Blood Mononuclear Cells From Coronavirus Disease 2019 PatientsCell and Developmental Biology Published on 2021-02-052022-10-31 Journal: Frontiers in Cell and Developmental Biology [Category] COVID-19, [키워드] activated acute respiratory syndrome co-culture Concentration conditions Contact coronavirus coronavirus disease COVID-19 COVID-19 patient COVID-19 patients cytokine dental dental pulp stem cells deregulated DPSC DPSCs form GM-CSF granulocyte healthy subject Human hyper-inflammation IFNγ IL-10 IL-12 IL-13 IL-17A IL-18 IL-1β IL-2 IL-21 IL-22 IL-23 IL-27 IL-4 IL-5 IL-6 IL-9 immune response immunomodulation in vitro interleukin investigated Macrophage mechanism mesenchymal stem cell Mesenchymal stem cells modulate modulation mononuclear cell MSC MSCs Multi-organ damage not affected Patient PBMC PBMCs peripheral Peripheral blood Pneumonia Production promote Pulp Real-time PCR Result SARS-CoV-2 STEM stem cell subset supernatant tissue regeneration TNFα [DOI] 10.3389/fcell.2020.609204 PMC 바로가기 [Article Type] Cell and Developmental Biology
Real Time PCR and Culture-Based Virus Isolation Test in Clinically Recovered Patients: Is the Subject Still Infectious for SARS-CoV2?Article Published on 2021-01-152022-10-30 Journal: Journal of Clinical Medicine [Category] COVID-19, [키워드] absence age Analysis Asymptomatic asymptomatic patient average Biosafety Level 3 Cell culture chain clearance clinically conducted contagious COVID-19 COVID-19 disease criteria Culture discharge hospitalized patient hospitalized patients in vitro Laboratory N gene nasopharyngeal Nasopharyngeal swab P/F ratio PaO Patient patients hospitalized PCR performed persistence Pneumonia positive positive RT-PCR public health reaction recovered RT-PCR SARS-CoV-2 Severe case specimen Still subject supernatant Swab swabbing Symptoms Test time viral clearance viral culture viral fragment viral growth Viral transmission virus [DOI] 10.3390/jcm10020309 PMC 바로가기 [Article Type] Article