Evaluation of the Serologic Cross-Reactivity between Transmissible Gastroenteritis Coronavirus and Porcine Respiratory Coronavirus Using Commercial Blocking Enzyme-Linked Immunosorbent Assay KitsResearch Article Published on 2019-03-132022-10-30 Journal: mSphere [Category] Coronavirus, MERS, [키워드] Accuracy Affect Analysis antibodies antibody Antigen blocking collected comparison Conventional cross-reactivity Culture current diagnostic early stage ELISA ELISA kit ELISAs enzyme-linked immunosorbent assay evaluated False positive gastroenteritis include individual infections inoculated measure medium Miller observation period porcine respiratory coronavirus positive Prevent proportion quarantine reported Research respiratory Respiratory Coronavirus S protein sensitivity serologic serological test serology serum serum antibodies serum sample specificity swine TGEV transmissible gastroenteritis virus [DOI] 10.1128/mSphere.00017-19 PMC 바로가기 [Article Type] Research Article
Decline of transmissible gastroenteritis virus and its complex evolutionary relationship with porcine respiratory coronavirus in the United StatesArticle Published on 2019-03-082022-10-29 Journal: Scientific Reports [Category] Coronavirus, MERS, [키워드] Affect amino acid change amino acid changes Characteristics circulating Complete complex Coronaviruses Decline Deletion dominant Endemic Epidemiology Evolution gastroenteritis Genetic genetic diversity Genome Genotype investigated occurred Occurrence Prevalence provide ranged real time recombination event Respiratory Coronavirus RT-PCR strain TGEV the United State unique variant virus [DOI] 10.1038/s41598-019-40564-z PMC 바로가기 [Article Type] Article
Porcine epidemic diarrhea virus S1 protein is the critical inducer of apoptosisResearch Published on 2018-11-072022-10-28 Journal: Virology Journal [Category] Coronavirus, MERS, [키워드] accompanied Activation age AIFM1 analyzed Apoptosis Apoptosis-inducing factor mitochondria associated 1 (AIFM1) caspase-3 caspase-8 caspase-9 caused Cell cell membrane characterized cleavage Concentration contagious contribute coronavirus Coronavirus infection Critical cytopathic effects demonstrated diarrhea disease effective epidemic diarrhea expression flow cytometry functional protein high mortality host cell host cells IBV immunofluorescence assay in vitro in vivo induce inducer infect infected cell infected cells inhibit less MERS molecular non-structural protein Nsp1–16 nuclear ORF3 overexpressing overexpression PARP PEDV Plasmid porcine epidemic diarrhea virus (PEDV) Protein Result S1 and S2 S1 protein spike spike S1 protein structural protein supplementary material syncytium formation tested TGEV the S1 protein transfected cells Vero VERO-E6 Vero-E6 cell virus was measured watery wild-type strain [DOI] 10.1186/s12985-018-1078-4 PMC 바로가기 [Article Type] Research
Porcine transmissible gastroenteritis virus nonstructural protein 2 contributes to inflammation via NF-κB activationResearch Paper Published on 2018-10-292022-10-28 Journal: Virulence [Category] Coronavirus, [키워드] activated activating acute enteritis Affect Amino acid amino acid residue bovine cause caused Cell contribute Critical Culture demonstrated domain down-regulation Eagle epithelial cell line exhibited expression fetal gastroenteritis indicated individual Infection infections Inflammation Inflammatory response inhibitor intestinal intestinal inflammation involved IκB monoclonal Necrosis NF-κB NF-κB NF-κB activation nitric oxide NSP2 nuclear p65 Pathogenesis pro-inflammatory cytokine pro-inflammatory cytokines Protein Regulation Replication reporter reverse transcription significantly suggested TGEV These data tissue transcription factor transmissible gastroenteritis virus Viral protein virus [DOI] 10.1080/21505594.2018.1536632 PMC 바로가기 [Article Type] Research Paper
Transferrin receptor 1 is a supplementary receptor that assists transmissible gastroenteritis virus entry into porcine intestinal epitheliumResearch Published on 2018-10-202022-10-31 Journal: Cell Communication and Signaling : CCS [Category] Coronavirus, [키워드] age Anemia antibody assist blocked Caco-2 cell cell line Clustering diarrhea down-regulated epithelial cell expressed expression Extracellular gastroenteritis Hypothesis infect Infection inhibited interact intestinal epithelium Invasion investigated IPEC-J2 cells mechanism mortality rate Newborn over-expressed primary target cell promoted Protein receptor Replication Result S1 protein significantly supplementary material Support TGEV transferrin receptor 1 transmissible gastroenteritis virus viral infection virus entry vomiting [DOI] 10.1186/s12964-018-0283-5 PMC 바로가기 [Article Type] Research
Molecular detection of enteric viruses and the genetic characterization of porcine astroviruses and sapoviruses in domestic pigs from Slovakian farmsResearch Article Published on 2018-10-192022-10-28 Journal: BMC veterinary research [Category] Coronavirus, [키워드] age circulating clinically collected diagnostic value diarrhea epidemic diarrhea European evaluate FIVE gastroenteritis Genetic genome sequence groups Health healthy Impact indicated Infection investigated less Lineage MOST no significant difference PEDV Phylogenetic analysis pig Porcine astrovirus Porcine sapovirus provide RdRP rectal swab Result risk sequence Slovakia strain TGEV virus virus strain [DOI] 10.1186/s12917-018-1640-8 PMC 바로가기 [Article Type] Research Article
Resistance to coronavirus infection in amino peptidase N-deficient pigsOriginal Paper Published on 2018-10-122022-10-28 Journal: Transgenic Research [Category] Coronavirus, [키워드] absence age alphacoronaviruses ANPEP caused collected coronavirus Coronavirus infection CRISPR/Cas9 disease Disease resistance Enterocyte enterocytes epidemic diarrhea evaluated gastroenteritis Genetic implication Infection knockout morbidity and mortality Necrosis Neonatal PCR PEDV phenotype premature stop codon putative receptor reactivity receptor resistance resulting retained supplementary material Support susceptibility Swab TGEV Viral receptor virus virus nucleic acid was determined wild type [DOI] 10.1007/s11248-018-0100-3 PMC 바로가기 [Article Type] Original Paper
Differentially expressed non-coding RNAs induced by transmissible gastroenteritis virus potentially regulate inflammation and NF-κB pathway in porcine intestinal epithelial cell lineResearch Article Published on 2018-10-122022-10-28 Journal: BMC Genomics [Category] Coronavirus, [키워드] activate Activation Analysis analyzed binding cell line cells circular RNA differentially expressed Effects epithelial epithelial cell line expressed gastroenteritis Infection Inflammation inhibited Interaction intestinal intestinal epithelial cell KEGG Kyoto microRNA miRNA miRNAs mitochondrial mRNA mRNAs ncRNA ncRNAs Next-generation sequencing NF-κB NF-κB pathway NGS non-coding RNA nucleus opposite overexpression p65 pathway Pathways Phosphorylation profile promoted Protein receptor reduction regulate reporter Result RNA severe inflammation supplementary material TGEV TNF translated transmissible gastroenteritis virus viral infection virus was used [DOI] 10.1186/s12864-018-5128-5 PMC 바로가기 [Article Type] Research Article
Establishment of porcine enterocyte/myofibroblast co-cultures for the growth of porcine rota- and coronavirusesArticle Published on 2018-10-122022-10-29 Journal: Scientific Reports [Category] Coronavirus, [키워드] bovine can be used Cell Characteristics co-culture co-cultured contributing to coronavirus Culture demonstrated enteric virus Enterocyte epithelial cells evaluated Extracellular matrix fecal fetal Genotype growth growth factor infect Last Miller PEDV proliferation Replication rotavirus strain secrete serum strain subepithelial susceptibility susceptible TGEV Vero viruses [DOI] 10.1038/s41598-018-33305-1 PMC 바로가기 [Article Type] Article
First retrospective studies with etiological confirmation of porcine transmissible gastroenteritis virus infection in ArgentinaResearch Article Published on 2018-09-242022-10-28 Journal: BMC veterinary research [Category] Coronavirus, MERS, [키워드] animal Antibiotics Antigen case sery characterized circulating Clinical diagnosis Clinical signs Complete coronavirus criteria detect determine Diagnosis diarrhea distribution Endemic epidemiological etiological evaluated Final gastroenteritis granular histological immunohistochemistry increase in Infection intestine lesion Microscopy monoclonal morbidity Mortality mRNA neonatal diarrhea Neonatal Mortality organization Particle pathogen PEDV Piglets Porcine transmissible gastroenteritis virus Region respond Result Retrospective study serological service Spike protein supplementary material TGEV viral infection virus virus infection was performed watery [DOI] 10.1186/s12917-018-1615-9 PMC 바로가기 [Article Type] Research Article