Impact of SARS-CoV-2 RBD Mutations on the Production of a Recombinant RBD Fusion Protein in Mammalian CellsArticle Published on 2022-08-242022-11-16 Journal: Biomolecules [Category] SARS, 변종, [키워드] accumulation assist B.1.351 B.1.351 variant cells chaperone chaperones contribute correlated COVID-19 diagnostic diagnostics disulphide bond domain E484K E484K mutation effort Endoplasmic reticulum expressed fusion Impact interfere mammalian mammalian cells misfolding mutant Mutation PDIA2 producing Production Protein protein disulphide isomerase protein yield. protocols RBD RBD mutation RBD protein RBD-Fc fusion protein recombinant recombinant RBD protein reduced resulting SARS-CoV-2 SARS-CoV-2 RBD SARS-CoV-2 variant secretion the RBD unfolded protein response UPR upregulation Vaccine variants [DOI] 10.3390/biom12091170 PMC 바로가기
Surface charge changes in spike RBD mutations of SARS-CoV-2 and its variant strains alter the virus evasiveness via HSPGs: A review and mechanistic hypothesisArticle Published on 2022-08-242022-11-16 Journal: Frontiers in Public Health [Category] SARS, 변종, 유전자 메커니즘, [키워드] Affect Alter antibodies antibody binding binding affinity calculated cells change changes changes in Characteristics complexes contagious COVID-19 pandemic distribution docking domain drug design electrostatic electrostatic interaction ENhance entry of SARS-CoV-2 epitope hACE2 HSPGs human Angiotensin-converting enzyme hypothese Hypothesis immune evasion Invasion involved molecular mechanism mutant Mutation mutations neutralizing antibody occurred omicron pathophysiological Point mutation positive Protein Proteoglycan RBD RBD mutation RBD mutations residue S protein S trimer SARS-CoV-2 SARS-CoV-2 mutation SARS-CoV-2 variants strain surface the RBD Therapies Vaccine development variant variant strain viral fitness viral infectivity virulence virus virus receptor [DOI] 10.3389/fpubh.2022.952916 PMC 바로가기
Genetically Engineered MRI-Trackable Extracellular Vesicles as SARS-CoV-2 Mimetics for Mapping ACE2 Binding In VivoArticle Published on 2022-08-232022-11-16 Journal: ACS Nano [Category] SARS, 변종, 유전자 메커니즘, [키워드] ACE2 ACE2 receptor approach binding complex coronavirus event EVs Extracellular extracellular vesicle Genetic genetic engineering hazard in vivo Interaction interactions ligand−receptor binding mapping mechanism MRI Mutagenesis mutant pandemic pathogenic virus platform predicted Protein raised RBD Receptor binding domain Safe SARS-CoV-2 subjects superparamagnetic iron oxide nanoparticles (SPIONs) the RBD tissues variant virus virus mimetics. viruses [DOI] 10.1021/acsnano.2c03119 PMC 바로가기
Cross-Neutralization of SARS-CoV-2-Specific Antibodies in Convalescent and Immunized Human Sera against the Bat and Pangolin Coronaviruses박쥐 및 천산갑 코로나바이러스에 대한 회복기 및 면역화된 인간 혈청에서 SARS-CoV-2-특이적 항체의 교차 중화Article Published on 2022-08-162022-09-12 Journal: Viruses [Category] SARS, 진단, [키워드] ACE2 analyzed appear bat bat coronavirus binding Cell cell-cell fusion chimeric chimeric proteins contribute coronavirus COVID-19 COVID-19 patient cross-neutralize highest Human human ACE2 receptor human host immunized inactivated induce less neutralization neutralization activity neutralized Neutralizing Neutralizing activity Neutralizing antibodies neutralizing antibody overexpressing pandemic pangolin pangolin coronavirus pangolins pre-existing immunity promote Protein pseudo-virus pseudo-virus. RaTG13 RBD replaced sarbecovirus SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 vaccine serum sample significantly significantly higher spike the RBD the receptor-binding domain thought transmitted vaccination VeroE6 cells while widespread [DOI] 10.3390/v14081793 PMC 바로가기 [Article Type] Article
Antibodies induced by an ancestral SARS-CoV-2 strain that cross-neutralize variants from Alpha to Omicron BA.1Alpha에서 Omicron BA.1까지 변이체를 교차 중화하는 조상 SARS-CoV-2 균주에 의해 유도된 항체Article Published on 2022-08-122022-09-11 Journal: Science Immunology [Category] Fulltext, SARS, 변종, 진단, [키워드] ACE2 binding antibodies antibody B cell binding binding site breadth caused Cell Clusters complexes cross-neutralize Defense defined Delta determinant determinants determine disease driven by Efficacy escape Fab FIVE greater highest Host host defense identifying Immune escape immune protection Infection initial memory B memory B cell Mitigation Mutation neutralization neutralization potency neutralize neutralized Neutralizing activity Neutralizing antibodies neutralizing antibody omicron Prevalence RBD real-world data Receptor binding domain recognize recognizing Region SARS-CoV-2 SARS-CoV-2 spike glycoprotein SARS-CoV-2 strain sensitivity Specificities specificity spike glycoprotein Spike receptor binding domain Structure structures tested the RBD the SARS-CoV-2 Vaccine vaccine efficacy variant variants variants of concern Viral viral escape viral invasion Wuhan strain [DOI] 10.1126/sciimmunol.abo3425 PMC 바로가기 [Article Type] Article
Targeting the Receptor-Binding Motif of SARS-CoV-2 with D-Peptides Mimicking the ACE2 Binding Helix: Lessons for Inhibiting Omicron and Future Variants of ConcernACE2 결합 나선을 모방한 D-펩타이드로 SARS-CoV-2의 수용체 결합 모티프 표적화: 오미크론 및 우려되는 미래 변이를 억제하기 위한 교훈Article Published on 2022-08-082022-09-11 Journal: Journal of Chemical Information and Modeling [Category] COVID19(2023년), SARS, 변종, [키워드] ACE2 ACE2 binding Alpha approach B.1.1.529 Beta binding blocked concern COVID-19 pandemic demonstrated domain Future high-affinity binding IC50 IC50s in vitro In vitro infection Infection inhibiting inhibitor lesson motif Mutation neutralizing monoclonal antibodies omicron Omicron variant overlapping predicted public interest RBD receptor-binding motif SARS-CoV-2 Spike protein Spread targeting the RBD the spike protein variant VoC VOCs [DOI] 10.1021/acs.jcim.2c00500 PMC 바로가기 [Article Type] Article
Conformational flexibility in neutralization of SARS-CoV-2 by naturally elicited anti-SARS-CoV-2 antibodies자연적으로 유도된 항-SARS-CoV-2 항체에 의한 SARS-CoV-2 중화의 구조적 유연성Article Published on 2022-08-052022-09-11 Journal: Communications Biology [Category] SARS, 변종, 진단, [키워드] ACE2 ACE2 binding site Alpha anti-RBD anti-SARS-CoV-2 anti-SARS-CoV-2 antibody antibody Arm array Beta bind binding conformations convalescent Cross linking cross-neutralizing Culture Delta Donor elicited epitope evaluate exhibit FIVE flexible Gamma likelihood mAb mAbs mechanism molecular monoclonal antibodies monoclonal antibody mutated neutralization neutralize Neutralizing nine omicron Proteins provide pseudoviruses RBD Receptor binding domain recruited SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 spike trimer SARS-CoV-2 variants Spike protein Spike proteins spike trimer Structure structures subunits tested the RBD the SARS-CoV-2 unique variant variants of concern variants of SARS-CoV-2 Viral viral evolution virus viruses VoC VOCs wild type Wuhan-Hu-1 [DOI] 10.1038/s42003-022-03739-5 PMC 바로가기 [Article Type] Article
A nanoparticle-based COVID-19 vaccine candidate elicits broad neutralizing antibodies and protects against SARS-CoV-2 infectionArticle Published on 2022-08-012022-11-15 Journal: Nanomedicine : nanotechnology, biology, and medici [Category] COVID19(2023년), SARS, 변종, 진단, [키워드] antibodies Antibody titer Antigen binding domain COVID-19 vaccine Delta elicit generate immune response immunization in vitro Infection K18-hACE2 transgenic mice mice neutralization neutralized neutralizing antibody omicron Papaya Papaya mosaic virus (PapMV) platform PROTECT RBD Receptor binding domain Receptor binding domain (RBD), Sortase (SrtA), broad protection Rod-shaped nanoparticle SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 variant susceptible T-cell the RBD Vaccine vaccine candidate vaccine platform variants viral respiratory infections virus [DOI] 10.1016/j.nano.2022.102584 PMC 바로가기
Insights into the structural peculiarities of the N-terminal and receptor binding domains of the spike protein from the SARS-CoV-2 Omicron variantSARS-CoV-2 Omicron 변이체의 스파이크 단백질 N-말단 및 수용체 결합 도메인의 구조적 특성에 대한 통찰력Article Published on 2022-08-012022-09-11 Journal: Computers in biology and medicine [Category] COVID19(2023년), SARS, 변종, [키워드] Accessibility ACE2 addition Analysis antibody B.1.1.529 BA.1) binding binding domain binding efficiency changes in computational analysis COVID-19 COVID-19 vaccines Effect Epitopes evaluated free energy glycan glycans higher infectivity insight Interaction mAb molecular dynamics molecular dynamics simulations Mutation N-terminal N-terminal domain N-terminal domain (NTD) Neutralizing NTD NTDs omicron Omicron (B.1.1.529 Omicron variant performed Protein provide raised RBD RBD domain RBDs Receptor binding domain receptor binding domain (RBD) reduce reducing residue SARS-CoV-2 specific antibodies Spike protein stability the RBD the SARS-CoV-2 the spike protein variant variant of SARS-CoV-2 Wuhan [DOI] 10.1016/j.compbiomed.2022.105735 PMC 바로가기 [Article Type] Article
Serological response to a third booster dose of BNT162b2 COVID-19 vaccine among seronegative cancer patients혈청 음성 암 환자에서 BNT162b2 COVID-19 백신의 3차 추가 접종에 대한 혈청학적 반응Article Published on 2022-08-012022-09-11 Journal: Cancer Reports [Category] COVID19(2023년), MERS, SARS, 진단, 치료기술, [키워드] Administered antibody Antibody Response anticancer BNT162b2 booster booster dose Cancer cancer patient COVID-19 COVID-19 vaccine detect domain dose elicit ELISA enzyme enzyme-linked immunosorbent enzyme-linked immunosorbent assay evaluated IgG IgG titers Immunoglobulin Immunoglobulin G of BNT162b2 Patient Patients with cancer Pfizer/BioNTech pilot study Receptor-binding domain Sample SARS-CoV-2 second vaccination serological response seronegative seropositive small sample size student t-test the RBD two-sided vaccine dose vaccine doses [DOI] 10.1002/cnr2.1645 PMC 바로가기 [Article Type] Article