Structure-based Design of a Specific, Homogeneous Luminescence Enzyme Reporter Assay for SARS-CoV-2SARS-CoV-2에 대한 특정 균질 발광 효소 리포터 분석의 구조 기반 설계Article Published on 2021-06-252022-09-11 Journal: Journal of Molecular Biology [Category] MERS, SARS, 진단, 치료기술, [키워드] antibody applied assays binding C-terminal catalytic activity complementary Complementation COVID-19 detectable develop diagnostic enzyme epitope Epitopes Express Gaussia Luciferase high affinity Incubation luciferase other diseases pathogenic PCA promise Protein protein-fragment complementation assay recombinant recombinant antibodies reporter reporters S-protein SARS CoV SARS CoV-2 SARS-CoV-2 SARS-CoV-2 antigen SARS-CoV-2 virus sensitivity Specific specificity spike spike (S) protein steric the SARS-CoV-2 virus threat Treatment Trimers Viral viral spike Virus-like particle virus-like particles [DOI] 10.1016/j.jmb.2021.166983 PMC 바로가기 [Article Type] Article
Potent neutralizing RBD‐specific antibody cocktail against SARS‐CoV‐2 and its mutantOriginal Article Published on 2021-06-242022-10-30 Journal: MedComm [Category] COVID-19, [키워드] ACE2 binding acute respiratory syndrome antibody antibody cocktail can be used caused cocktail Combination contagious coronavirus Cyro‐EM demonstrated described dissemination effective epitope epitopes group exhibited global public health group high affinity hotspot Intervention isolates mAb mAb cocktail mAbs mice monoclonal antibody mutant mutants neutralization Neutralizing neutralizing antibody pandemic potent Prevent Prophylactic Protein pseudotype pseudovirus RBD receptor‐binding domain receptor‐binding domain recognize recognizing reveal SARS‐CoV‐2 SARS‐CoV‐2 selected the RBD the spike protein therapeutic Transmission treat V367F vaccination Vaccine variant virus wildtype [DOI] 10.1002/mco2.79 PMC 바로가기 [Article Type] Original Article
Immunodominant T-cell epitopes from the SARS-CoV-2 spike antigen reveal robust pre-existing T-cell immunity in unexposed individualsSARS-CoV-2 스파이크 항원의 면역 우세 T 세포 에피토프는 노출되지 않은 개인에서 강력한 기존 T 세포 면역을 나타냅니다Article Published on 2021-06-232022-09-10 Journal: Scientific Reports [Category] MERS, SARS, 유전자 메커니즘, [키워드] Adaptive immunity Algorithm Antigen Asymptomatic CD4 CD8 T-cell clinical outcomes CMV common cold-causing coronaviruses contributed COVID-19 pandemic determine disease phenotype Donor donors epitope Epitopes exposure to flu Immunity immunodominant immunology individual infected patient infected patients Infection Influenza lack mechanism Mild outcome peptide peptide pool pre-existing T-cell predicted reactive reactivity recognize robust SARS-CoV-2 SARS-CoV-2 antigen SARS-CoV-2 epitopes sequence identity suggested T-cell T-cell activation T-cell epitope T-cells TCR TCRs the SARS-CoV-2 Viral Viral antigen viruses [DOI] 10.1038/s41598-021-92521-4 PMC 바로가기 [Article Type] Article
T cell epitopes of SARS-CoV-2 spike protein and conserved surface protein of Plasmodium malariae share sequence homologyResearch Article Published on 2021-06-232022-10-28 Journal: Open Life Sciences [Category] COVID-19, [키워드] acute respiratory syndrome Amino acid Analysis approach B cell conserved coronavirus descendant epitope IEDB Immune Epitope Database immune response Immunity immunodominant epitope immunogenic profile information malaria MONITOR motif Pairwise comparison pathogen Phylogenetic analysis Phylogenetic relationship Plasmodium malariae proteome prototype replaced SARS-CoV SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 spike protein sequence sequence homology Seven spike glycoprotein Spread surface protein T cell epitope the spike protein [DOI] 10.1515/biol-2021-0062 PMC 바로가기 [Article Type] Research Article
The Fc-mediated effector functions of a potent SARS-CoV-2 neutralizing antibody, SC31, isolated from an early convalescent COVID-19 patient, are essential for the optimal therapeutic efficacy of the antibody초기 회복기 COVID-19 환자로부터 분리 된 강력한 SARS-COV-2 중화 항체, SC31의 FC- 매개 이펙터 기능은 항체의 최적의 치료 효능에 필수적이다.Research Article Published on 2021-06-232022-08-13 Journal: PLoS ONE [Category] Biochemistry, MERS, SARS, 변종, 유전자 메커니즘, 진단, [키워드] ACE2 addition Administered animal models Anti-SARS-CoV-2 Activity anti-viral immune responses antibody Antibody-dependent enhancement Antibody-mediated neutralization convalescent convalescent patient COVID-19 COVID-19 patient demonstrated dose-dependent efficacy effective Efficacy epitope evidenced exhibited Fc-mediated effector function Fc-mediated effector functions Fc-null LALA variant Histopathology IFNγ immune responses Inflammatory responses K18-human ACE2 transgenic mice LALA variant Lower respiratory tract lung Lungs mice neutralization ability Neutralizing antibodies neutralizing antibody occur preclinical studies preclinical study prevented pro-inflammatory response pro-inflammatory responses promote Protein Receptor-binding domain reduced viral load reduced viral loads reduction respiratory rhesus macaques SARS-CoV-2 SARS-CoV-2 neutralizing antibody SARS-CoV-2 spike protein SARS-CoV-2-infected hamsters SARS-CoV-2-neutralizing antibodies SARS-CoV-2-neutralizing antibody SC31 SC31 treatment severity of COVID-19 significantly spike Spike protein sub-therapeutic doses the receptor-binding domain the SARS-CoV-2 therapeutic therapeutic efficacy therapeutic potential Therapeutics transgenic mice treated treatment with SC31 undetectable Viral Viral load viral loads viral-induced viral-induced lung inflammatory responses weight loss [DOI] 10.1371/journal.pone.0253487 PMC 바로가기 [Article Type] Research Article
Immune Evasion of SARS-CoV-2 Emerging Variants: What Have We Learnt So Far?SARS-COV-2 신흥 변종의 면역 회피 : 지금까지 무엇을 배웠습니까?Review Published on 2021-06-222022-09-01 Journal: Viruses [Category] MERS, SARS, 변종, 진단, [키워드] accumulation Alpha antibody B.1.1.7 B.1.351 B.1.617 B.1.617.2 Beta Characteristics close monitoring convalescent individual convalescent individuals COVID-19 COVID-19 pandemic Delta Diversity elicited epitope Epitopes Epsilon evade Evolutionary rate Gamma genetic diversity human population Immune escape immune evasion Infection Lineage monoclonal antibodies Mutation mutations neutralization neutralization capacity P.1 recipient recipients reduced RNA viruses SARS-CoV-2 sera Spike protein the spike protein therapeutic Transmission vaccination Vaccines Variability variant variant of concern variants VoC while [DOI] 10.3390/v13071192 PMC 바로가기 [Article Type] Review
Isolation and characterization of cross-neutralizing coronavirus antibodies from COVID-19+ subjectsCOVID-19+ 대상에서 교차 중화 코로나바이러스 항체의 분리 및 특성화Article Published on 2021-06-222022-09-11 Journal: Cell Reports [Category] MERS, SARS, 변종, 진단, [키워드] Abstract ACE2 receptor anti-RBD anti-S2 anti-SARS-CoV-2 anti-SARS-CoV-2 antibodies antibodies antibody B.1.351 B.1.351 variant binding caused coronavirus coronavirus vaccine cross-neutralize cross-neutralizing cross-neutralizing antibody CV3-25 disease epitope Epitopes human coronavirus identify immunogen immunogens K18-hACE mAb mice monoclonal antibodies mouse mutant N-terminal domain NAb neutralization neutralization potency neutralize neutralized Neutralizing Neutralizing antibodies neutralizing antibody NTD Pandemics Patient patients Prevent Protective protective effect RBD Receptor-binding domain recognize regulate reveal S2 subunit SARS-CoV-1 SARS-CoV-2 SARS-CoV-2 neutralizing antibodies SARS-CoV-2 RBD specificity subject target the receptor-binding domain Transgenic mouse Vaccines widespread [DOI] 10.1016/j.celrep.2021.109353 PMC 바로가기 [Article Type] Article
The landscape of antibody binding in SARS-CoV-2 infectionSARS-COV-2 감염에서 항체 결합의 풍경Research Article Published on 2021-06-182022-08-13 Journal: PLoS Biology [Category] Biochemistry, MERS, SARS, 진단, 치료법, [키워드] Accuracy acute respiratory syndrome acute respiratory syndrome coronavirus antibody Antibody binding B cell epitope B cell epitopes binding convalescent coronavirus correlated CoV proteins COVID-19 CoVs cross-reactivity develop diagnostic Diagnostic accuracy diagnostics ELISA enzyme enzyme-linked immunosorbent enzyme-linked immunosorbent assay epitope Epitopes homologous human CoVs human sera humoral immunogen humoral immunogens identify illness Illness severity induce membrane naïve nucleocapsid occurred ORF3a ORF8 protein ORF8 proteins other coronavirus other coronaviruses pandemic peptide peptide sequences Profiling Protein Proteins proteome reactive reactivity respiratory robust antibody response robust antibody responses SARS-CoV-2 SARS-CoV-2 epitope SARS-CoV-2 epitopes SARS-COV-2 infection SARS-CoV-2 proteome sequence severity the SARS-CoV-2 Therapeutics ultradense peptide microarray Vaccines [DOI] 10.1371/journal.pbio.3001265 PMC 바로가기 [Article Type] Research Article
Landscape of epitopes targeted by T cells in 852 individuals recovered from COVID-19: Meta-analysis, immunoprevalence, and web platformCOVID-19에서 회복된 852명의 T 세포가 표적으로 하는 에피토프의 풍경: 메타 분석, 면역 유병률 및 웹 플랫폼Meta-Analysis Published on 2021-06-152022-09-11 Journal: Cell Reports Medicine [Category] SARS, 변종, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Analysis CD4 CD8 Cohort cohorts complement convalescent Convalescent patients coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 COVID-19 vaccine COVID-19 vaccines diagnostic diagnostics epitope Epitopes fraction help identify Immunity immunodominant immunodominant epitopes immunological Immunological studies immunoprevalent epitopes individual individuals information landscape majority Meta-analysis platform population coverage recorded SARS-CoV-2 severe acute respiratory syndrome Coronavirus T cell T cell epitope T cell epitopes T cell response T cell responses T cells tested Vaccine variants variants of concern web-based platform [DOI] 10.1016/j.xcrm.2021.100312 PMC 바로가기 [Article Type] Meta-Analysis
Multiple Roles of SARS-CoV-2 N Protein Facilitated by Proteoform-Specific Interactions with RNA, Host Proteins, and Convalescent AntibodiesArticle Published on 2021-06-152022-10-30 Journal: JACS Au [Category] COVID-19, [키워드] antibodies antigenic approved bind C-terminal C-terminus convalescent convalescent plasma coronavirus COVID-19 cyclosporin A epitope exhibited functional highlight identify IgA antibody IgG IgM immunogenic Interaction mass spectrometry motif motifs Multiple N protein N-terminal nucleocapsid Protein Proteolytic processing Replication ribonucleoprotein RNA role SARS-CoV-2 SARS-CoV-2 N SARS-CoV-2 nucleocapsid significantly therapeutic intervention Therapies Viral protein virion assembly virus [DOI] 10.1021/jacsau.1c00139 PMC 바로가기 [Article Type] Article