Porcine epidemic diarrhea virus E protein causes endoplasmic reticulum stress and up-regulates interleukin-8 expressionResearch Published on 2013-01-192022-10-28 Journal: Virology Journal [Category] Coronavirus, [키워드] activated addition BCL-2 cause Cell cell cycle progression cells E protein Effect Endoplasmic reticulum epidemic diarrhea ER stress Express expressing expression function growth had no IEC IL-8 intestinal epithelial cell investigated NF-Κb NF-κB nucleus pathogen PEDV previous study Research responsible Result Stress subcellular localization survival the cell up-regulate virus [DOI] 10.1186/1743-422X-10-26 PMC 바로가기 [Article Type] Research
Recombinant parainfluenza virus 5 vaccine encoding the influenza virus hemagglutinin protects against H5N1 highly pathogenic avian influenza virus infection following intranasal or intramuscular vaccination of BALB/c miceArticle Published on 2013-01-012022-10-06 Journal: Journal of Virology [Category] 진단, [키워드] administration Antigen approach approach BALB/c BALB/c mice booster contraindication different effectively controlled Efficacy ENCODE expressing H5N1 highly pathogenic immune responses immunization inactivated Infection Influenza Influenza virus intramuscular intramuscular vaccination intranasal lung mucosal IgA response Neutralizing New pandemic parainfluenza virus Prevent PROTECT Protective recombinant required robust serum antibody response shown single vaccine tested the vaccine threat vaccination Vaccine Vaccine development Vaccines virion Virology virus virus replication viruses while [DOI] 10.1128/JVI.02330-12 PMC 바로가기 [Article Type] Article
Human Monoclonal Antibodies against Highly Conserved HR1 and HR2 Domains of the SARS-CoV Spike Protein Are More Broadly NeutralizingResearch Article Published on 2012-11-212022-10-28 Journal: PLoS ONE [Category] Coronavirus, [키워드] Amgen antibody binding conserved convalescent patient different ectodomain effective expressing glycoprotein Human Human monoclonal antibody immune implication Infected isolates monoclonal antibody neutralize Neutralizing Patient produced pseudotyped virus Receptor binding domain Region respiratory S protein S1 domain S2 domain SARS-CoV SARS-CoV spike sequence sera severe shown Subunit vaccine the S protein therapeutic therapy Treatment variant viruses [DOI] 10.1371/journal.pone.0050366 PMC 바로가기 [Article Type] Research Article
In vitro inhibition of transmissible gastroenteritis coronavirus replication in swine testicular cells by short hairpin RNAs targeting the ORF 7 geneResearch Published on 2012-08-282022-10-28 Journal: Virology Journal [Category] Coronavirus, [키워드] Apoptosis approach assays Cell cell proliferation characterized contagious Coronavirus replication Cytopathic effect demonstrated diarrhea effective Efficiency Evidence examined expressing expression expression plasmid expression plasmids gastroenteritis Genome high mortality in vitro indicated Infection inhibit inhibited inhibitory effect N gene ORF ORF 7 Quantitative Replication reporter Result RNA RNA interference RT-PCR analysis selected sequence short hairpin RNA shown significantly specificity STABLE target gene TCID TGEV the vaccine therapeutic transmissible gastroenteritis virus Treatment viral disease viral infections viral replication virus virus replication virus titer vomiting [DOI] 10.1186/1743-422X-9-176 PMC 바로가기 [Article Type] Research
Reverse Genetics of SARS-Related Coronavirus Using Vaccinia Virus-Based RecombinationResearch Article Published on 2012-03-072022-10-28 Journal: PLoS ONE [Category] Coronavirus, [키워드] absence acute respiratory syndrome Analysis antiviral drug approach caused cDNA Cell characterization classical cloned Comparative analysis culture supernatant dendritic cell effective expressing facilitate Gene Expression generate Genome HCoV-229E Health Heterologous human coronavirus 229E in vitro transcription infectious Infectious virus led to less original form outbreak parental pathogenic plaque produced public health measures released Renilla luciferase Renilla luciferase gene Replication replication kinetics reporter reverse genetic system risk RNA transcript RNA transcripts robust SARS-CoV SARS-CoV Frankfurt-1 SARS-related coronavirus severe transfection Vaccines vaccinia vaccinia virus vectors virulent virus zoonotic disease [DOI] 10.1371/journal.pone.0032857 PMC 바로가기 [Article Type] Research Article
Coronavirus Papain-like Proteases Negatively Regulate Antiviral Innate Immune Response through Disruption of STING-Mediated SignalingResearch Article Published on 2012-02-012022-10-06 Journal: PLoS ONE [Category] 진단, [키워드] Activation acute respiratory syndrome Antiviral block catalytic catalytically active Cell cells complex complexes contributing to coronavirus CoV Defense disrupting Disruption domain Endoplasmic reticulum evade expressing expression form found HCoV-NL63 Host IFN IKKε inactive Infected inhibit Innate innate immune innate immune response interferon MAVS mechanism membrane mutant NL63 nuclear translocation Papain-like protease PLP promoters Protein reduced regulate required response RIG-I SARS-CoV Signaling signaling molecule subsequent TBK1 wild type [DOI] 10.1371/journal.pone.0030802 PMC 바로가기 [Article Type] Research Article
SARS Coronavirus 3b Accessory Protein Modulates Transcriptional Activity of RUNX1bResearch Article Published on 2012-01-122022-10-28 Journal: PLoS ONE [Category] Coronavirus, [키워드] Accessory accessory protein activity acute respiratory syndrome Affect AP-1 binding Cell cells cellular chemokine co-immunoprecipitation cytokine dendritic cells downstream genes elevated ENCODE ERK expressing finding found functions Genome Host IL2 increase in Infected Infection inhibitor Interaction interferon isoform kinase lab led to MIP-1α monocyte mRNA level Pathogenesis pharmacological Phosphorylation physiological promoter Protein recruitment related reporter gene assay Runt RUNX1 SARS Coronavirus SARS virus SARS-CoV target gene transcription factor transcriptional activity Treatment unique upregulated upregulating upregulation yeast [DOI] 10.1371/journal.pone.0029542 PMC 바로가기 [Article Type] Research Article
Severe Acute Respiratory Syndrome Coronavirus Envelope Protein Regulates Cell Stress Response and ApoptosisResearch Article Published on 2011-10-202022-10-29 Journal: PLoS Pathogens [Category] Coronavirus, MERS, [키워드] absence activating Activation acute respiratory syndrome addition alleviate Apoptosis apoptotic Author Cell cell metabolism cell stress cells compared contribute contributed control cell different dissemination down-regulated drug drugs E gene E protein elicit envelope enzyme expressing expression Factor Gene Gene Expression homeostasis Host host gene expression identify immunoregulation in vivo increase in Infected Infection Inflammation involved lack mechanisms mutant pathway Pathways potential mechanism proinflammatory Proinflammatory cytokine PROTECT Protein reduced reduction reduction in regulated respiratory respiratory syncytial virus response reversed SARS-CoV SARS-CoV E severe Signal transduction signaling pathway Stress subset Transcription transcription factor treated Treatment unfolded protein response upregulated virus virus production was reduced [DOI] 10.1371/journal.ppat.1002315 PMC 바로가기 [Article Type] Research Article
A Recombinant Avian Infectious Bronchitis Virus Expressing a Heterologous Spike Gene Belonging to the 4/91 SerotypeResearch Article Published on 2011-08-302022-10-28 Journal: PLoS ONE [Category] Coronavirus, [키워드] absence backbone Belonging Clinical sign Clinical signs defined different ectodomain effective expressing Genetic Heterologous homologous IBV Infected M41 observation pathogenic pathogenicity qRT-PCR recombinant removed RNA S gene serotype shown spike the S protein the spike protein Trachea Vaccines virus wild type [DOI] 10.1371/journal.pone.0024352 PMC 바로가기 [Article Type] Research Article
SARS coronavirus protein 7a interacts with human Ap 4 A-hydrolaseResearch Published on 2010-02-092022-10-28 Journal: Virology Journal [Category] Coronavirus, [키워드] activated Activation Amino acid Apoptosis cell proliferation cellular co-immunoprecipitation culture cell cytoplasm DNA repair DNA replication ENCODE expressing Frame Host Human human cell interact Interaction involved nucleotide ORF pathogenic processe Protein protein synthesis Proteins Regulation responsible RNA SARS Coronavirus SARS-CoV sequence homology shown tissue virus yeast [DOI] 10.1186/1743-422X-7-31 PMC 바로가기 [Article Type] Research