Wide Application of Minimally Processed Saliva on Multiple RT-qPCR Kits for SARS-CoV-2 Detection in Indonesia인도네시아에서 SARS-CoV-2 검출을 위한 다중 RT-qPCR 키트에 최소 처리된 타액의 광범위한 적용Cellular and Infection Microbiology Published on 2021-08-182022-09-11 Journal: Frontiers in Cellular and Infection Microbiology [Category] MERS, SARS, 치료기술, 치료제, [키워드] applicability application assistance bypasse COVID-19 demonstrated described detection direct-PCR FIVE human saliva individual Indonesia large capacity Local Multiple nasopharyngeal nine oropharyngeal swab Real-time PCR reducing remained required RNA-extraction-free room temperature RT-qPCR Saliva saliva specimen saliva specimens SARS-CoV-2 specimen stability target gene Target genes temperature Treatment Variability was tested [DOI] 10.3389/fcimb.2021.691538 PMC 바로가기 [Article Type] Cellular and Infection Microbiology
Evaluation of Self-Collected Midturbinate Nasal Swabs and Saliva for Detection of SARS-CoV-2 RNASARS-CoV-2 RNA 검출을 위한 자가 수집 중비갑개 비강 면봉 및 타액 평가Article Published on 2021-08-182022-09-11 Journal: Journal of Clinical Microbiology [Category] SARS, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 authorization Care Consensus coronavirus COVID-19 COVID-19 pandemic detection diagnostic diagnostic testing Emergency Emergency use authorization exhibited extraction FDA Health Health care Heating individual investigated Laboratory midturbinate NAAT nasal swab nasopharyngeal NP swab NP swabs patients PCR assay positive proteinase Proteinase K Rapid reduced routine monitoring Saliva saliva specimen saliva specimens SARS-CoV-2 SARS-CoV-2 detection SARS-COV-2 infection SARS-CoV-2 RNA sensitivity severe acute respiratory syndrome Coronavirus specificity Supply shortages Swab swabs These data [DOI] 10.1128/JCM.00848-21 PMC 바로가기 [Article Type] Article
Saliva is Comparable to Nasopharyngeal Swabs for Molecular Detection of SARS-CoV-2타액은 SARS-CoV-2의 분자 검출을 위한 비인두 면봉과 비슷합니다.Research Article Published on 2021-08-182022-09-10 Journal: Microbiology Spectrum [Category] MERS, SARS, 진단, 치료기술, [키워드] a minor Abbott acquisition acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 alternative average clinical sensitivity Concordance coronavirus COVID-19 COVID-19 testing decrease detection drink Follow-up food fraction guanidinium individual infectious risk initial leak less limit of detection limits Limits of detection majority medium minor molecular nasopharyngeal Nasopharyngeal swab NP swab NP swabs Outpatient outpatients Patient platform presenting respiratory restriction reverse transcriptase reverse transcriptase PCR RT-PCR Saliva saliva specimen saliva specimens SARS-CoV-2 severe acute respiratory syndrome Coronavirus smoking specimen specimen collection Swab tested Transport transport medium treated Viral Viral load viral loads Viral RNA virus [DOI] 10.1128/Spectrum.00162-21 PMC 바로가기 [Article Type] Research Article
Direct detection of SARS-CoV-2 antisense and sense genomic RNA in human saliva by semi-autonomous fluorescence in situ hybridization: A proxy for contagiousness?반자동 형광에서 인간 타액에서 SARS-COV-2 안티센스 및 감각 게놈 RNA의 직접 검출 : 현장 하이브리드 화 : 전염성을위한 대리?Research Article Published on 2021-08-172022-09-10 Journal: PLoS ONE [Category] MERS, SARS, 유전자 메커니즘, 진단, 치료기술, 치료제, [키워드] addition All participants antisense applied Cell collected contagiousness cytological described diagnostic Direct DNA Fluorescence genomic genomic RNA healthy human saliva In situ hybridization information interpretability matrix Mild moderate Moderate COVID-19 Participants pathogen transmission pilot study positive presenting probe probes provide Saliva saliva samples SARS-CoV-2 SARS-CoV-2 RNA sense Symptoms test results tested the cells the healthy Validity vector Viral Viral load viral replication virus Visualization [DOI] 10.1371/journal.pone.0256378 PMC 바로가기 [Article Type] Research Article
Impact of Full Vaccination with mRNA BNT162b2 on SARS-CoV-2 Infection: Genomic and Subgenomic Viral RNAs Detection in Nasopharyngeal Swab and Saliva of Health Care WorkersArticle Published on 2021-08-142022-10-29 Journal: Microorganisms [Category] COVID-19, [키워드] analysed Analysis Asymptomatic B.1.1.7 variant baseline BNT162b2 Concordance coronavirus Course cumulative detection droplet digital PCR eight expression Frequency HCW HCWs Health care worker hospitals identify Impact incidence individual individuals infected individuals Infection investigated majority MONITOR mRNA BNT162b2 Mutation N501Y NPs performed positive provided real time real time RT-PCR real-time RT-PCR receiving resulting RNA RT-PCR Saliva saliva specimen SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 surveillance specimens subgenomic subjects symptomatic symptomatic cases tested Transmission vaccination Vaccine vaccinee variants Viral load Viral mutations Viral RNA was measured worker [DOI] 10.3390/microorganisms9081738 PMC 바로가기 [Article Type] Article
COVID-19 diagnosis by RT-qPCR in alternative specimens대체 표본에서 RT-qPCR에 의한 COVID-19 진단Original Article Published on 2021-08-132022-09-10 Journal: Memórias do Instituto Oswaldo Cruz [Category] MERS, SARS, 진단, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 alternative specimens asymptomatic subject cause Chain Reaction concordance rate Contamination coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 COVID-19 diagnosis cycle threshold Diagnosis discomfort gingival fluid gold gold standard healthcare worker Healthcare workers identify Inconclusive inherent Nasopharyngeal swab NP swab NP swabs pandemic patients polymerase chain polymerase chain reaction positive real-time polymerase chain reaction respiratory reverse transcription risk RT-qPCR Saliva saliva samples SARS-CoV-2 SARS-CoV-2 detection severe acute respiratory syndrome Coronavirus specimen Swab [DOI] 10.1590/0074-02760210085 PMC 바로가기 [Article Type] Original Article
A molecular test based on RT-LAMP for rapid, sensitive and inexpensive colorimetric detection of SARS-CoV-2 in clinical samples임상 샘플에서 SARS-COV-2의 신속하고 민감하며 저렴한 비색 검출을위한 RT-LAMP에 기초한 분자 시험Article Published on 2021-08-122022-09-01 Journal: Scientific Reports [Category] MERS, SARS, 진단, 치료기술, [키워드] clinical sample colorimetric detection COVID-19 diagnosis COVID-19 vaccination diagnostic test diagnostic tests disease spread disparate distribution DNA extraction effective enzyme Enzymes expected gold gold standard implementation inactivation independent independent of Infectious-disease diagnostics lack LAMP less Measures Molecular biology molecular test nasopharyngeal pandemic performed Plasmid Prevent problems produced protocol reagent reagents remained RNA RNA extraction RNA samples robust RT-LAMP RT-PCR Saliva saliva samples SARS-CoV-2 sensitivity slowdown specificity Supply chain Viral viral genome Viral load viral loads Visualization [DOI] 10.1038/s41598-021-95799-6 PMC 바로가기 [Article Type] Article
Developing methods to detect and diagnose chronic traumatic encephalopathy during life: rationale, design, and methodology for the DIAGNOSE CTE Research Project생애 동안 만성 외상성 뇌병증을 탐지하고 진단하는 방법 개발 : 진단 CTE 연구 프로젝트를위한 이론적 근거, 설계 및 방법론Multicenter Study Published on 2021-08-122022-09-10 Journal: Alzheimer's Research & Therapy [Category] MERS, SARS, 유전자 메커니즘, 치료기술, 치료제, [키워드] accelerate age anonymized data baseline biological sample biological samples Biomarker Biomarkers Blood blood draw Brain can not chronic Chronic traumatic encephalopathy Clinical research cognitive Cognitive function College football Community Concussion Consensus Contact COVID-19 pandemic detect develop Developing diagnose diagnosed Diagnosis diagnostic Diagnostic Criteria disease Donor Encephalopathy Enrollment Epidemiology evaluated examined facilitate finding Follow-up Football Genetic Head trauma imaging impacts in vivo life Lumbar puncture magnetic resonance imaging male participants Measures mechanisms methodology MRI MRS multicenter National Football League network neurodegenerative Neurodegenerative disease Neuroimaging neurologic neurological Neurological disorders Neuropsychiatric objective organized participant Participation positron emission Positron emission tomography reliability Remote assessment Repetitive head impacts Research Research diagnostic criteria resource Result risk Risk factors Saliva Sample size self-report measure Spectroscopy Subconcussion supplementary material syndrome Tau TES trauma Traumatic brain injury Traumatic encephalopathy syndrome Treatment Validity [DOI] 10.1186/s13195-021-00872-x PMC 바로가기 [Article Type] Multicenter Study
Long-term SARS-CoV-2-specific immune and inflammatory responses in individuals recovering from COVID-19 with and without post-acute symptoms급성 후 증상이 있거나 없는 COVID-19에서 회복하는 개인의 장기 SARS-CoV-2 특이적 면역 및 염증 반응Article Published on 2021-08-102022-09-11 Journal: Cell Reports [Category] MERS, SARS, 진단, 치료법, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 antibody antibody levels CD107a CD4 CD8 coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 degranulation expressing Frequency immune immune response immune responses Immunity individual Infection Inflammation Inflammatory response initial Long COVID Lung disease magnitude marker neutralization capacity observé participant PASC PCR-confirmed peptide peptide pool Post-acute sequelae of SARS-CoV-2 infection recovering from COVID-19 respiratory Saliva SARS-CoV-2 SARS-COV-2 infection severe acute respiratory syndrome Coronavirus severity Spectrum Symptom symptom onset T cell T cell response T cell responses T cells Viral viral shedding [DOI] 10.1016/j.celrep.2021.109518 PMC 바로가기 [Article Type] Article
Rapid and Efficient Detection of the SARS-CoV-2 Spike Protein Using an Electrochemical Aptamer-Based SensorArticle Published on 2021-08-102022-10-28 Journal: ACS Sensors [Category] COVID-19, [키워드] aptasensors biological fluid conformational change COVID-19 demonstrated detect detection diagnostic EAB sensors efficient electrochemical sensors infectious diseases platform Protein quantitative measurement Rapid real time Saliva SARS-CoV-2 selected Sensitivity and specificity sensor serum spike spread of COVID-19 tested the S protein the SARS-CoV-2 undergo [DOI] 10.1021/acssensors.1c01222 PMC 바로가기 [Article Type] Article