A novel biotinylated nanobody-based blocking ELISA for the rapid and sensitive clinical detection of porcine epidemic diarrhea virusResearch Published on 2019-09-162022-10-29 Journal: Journal of Nanobiotechnology [Category] Coronavirus, [키워드] antibody biotinylated Biotinylated-nanobody Blocking ELISA characterized cutoff value detect Diagnosis Diagnostic method diarrhea Efficacy ELISA epidemic diarrhea epidemiological evaluate the effect expression fusion protein high mortality rate immunization in vitro Infection N protein nanobody overcome PEDV periplasmic problem purified required Result Sensitivity and specificity serological serum serum sample stability strain tested time-consuming Vaccine virus vomiting watery [DOI] 10.1186/s12951-019-0531-x PMC 바로가기 [Article Type] Research
Serological Screening for Coronavirus Infections in CatsArticle Published on 2019-08-132022-10-28 Journal: Viruses [Category] Coronavirus, MERS, [키워드] addition Amino acid analyzed antibody antibody epitope antigenic Antisera cats Cell cell entry conserved coronavirus Coronavirus infection Coronaviruses CoV CoVs cross-reaction cross-reactivity Cross-species transmission diagnoses domain ELISA engage epidemic diarrhea Evidence excluded HCoV-229E human coronavirus 229E immunogenic indicated infect Infection mammal ortholog PEDV positive presence of antibody receptor Receptor binding reported S1 protein screened Screening sequence identity sera serological serum serum sample Spike protein subunit supported virus Virus neutralization widespread [DOI] 10.3390/v11080743 PMC 바로가기 [Article Type] Article
First retrospective studies with etiological confirmation of porcine transmissible gastroenteritis virus infection in ArgentinaResearch Article Published on 2018-09-242022-10-28 Journal: BMC veterinary research [Category] Coronavirus, MERS, [키워드] animal Antibiotics Antigen case sery characterized circulating Clinical diagnosis Clinical signs Complete coronavirus criteria detect determine Diagnosis diarrhea distribution Endemic epidemiological etiological evaluated Final gastroenteritis granular histological immunohistochemistry increase in Infection intestine lesion Microscopy monoclonal morbidity Mortality mRNA neonatal diarrhea Neonatal Mortality organization Particle pathogen PEDV Piglets Porcine transmissible gastroenteritis virus Region respond Result Retrospective study serological service Spike protein supplementary material TGEV viral infection virus virus infection was performed watery [DOI] 10.1186/s12917-018-1615-9 PMC 바로가기 [Article Type] Research Article
Development of a sensitive and specific xMAP assay for detection of antibodies against infectious laryngotracheitis and bronchitis virusesMethodology Published on 2018-09-212022-10-28 Journal: Virology Journal [Category] Coronavirus, MERS, [키워드] antibody bronchitis can be used coated with demonstrated detect detection system development differential diagnosis disease ELISA ELISA assays enzyme-linked immunosorbent exhibited glycoprotein IBV imperative incubated Infectious Bronchitis Virus Infectious bronchitis virus, antibody detection, ELISA Infectious laryngotracheitis virus Influenza virus Luminex xMAP technology microsphere multiplex nonspecific nucleocapsid Protein purified quantification reaction Result serological serum serum sample serum samples tested vaccination virus [DOI] 10.1186/s12985-018-1048-x PMC 바로가기 [Article Type] Methodology
Development and application of an indirect ELISA for the detection of antibodies to porcine epidemic diarrhea virus based on a recombinant spike proteinMethodology Article Published on 2018-08-202022-10-28 Journal: BMC veterinary research [Category] Coronavirus, [키워드] antibody applied caused China collected condition cross-reactivity detect development diagnose Diagnosis diarrhea ELISA enzyme-linked immunosorbent epidemic diarrhea IFA Infection pathogen PEDV Porcine epidemic diarrhea virus Protein raising Recombinant spike protein Result Sensitivity and specificity serological serum antibody serum sample Spike protein the disease virus [DOI] 10.1186/s12917-018-1570-5 PMC 바로가기 [Article Type] Methodology Article
Chaperna-Mediated Assembly of Ferritin-Based Middle East Respiratory Syndrome-Coronavirus NanoparticlesImmunology Published on 2018-05-172022-11-01 Journal: Frontiers in Immunology [Category] Coronavirus, MERS, [키워드] affected aggregation Antigen assembly Bacterial binding cellular receptor chaperone Concentration conformations contributed Control defined domain effective Escherichia coli expressed expression favor ferritin fused hDPP4 hosts immunization immunologically in silico in vitro Interaction kinetic lack linker Lysyl-tRNA synthetase MERS-CoV MERS-CoV RBD Microscopy Middle East Middle East respiratory syndrome Coronavirus molecular monomer monomeric Mutation nanoparticle pathway platform presenting proteolytic RBD Receptor-binding domain recombinant vaccine reducing respiratory RNA RNA-interacting domain of human LysRS robust scattering sera serological significantly increased stability Structure structures subsequent Vaccine viral infections Virus-like particle [DOI] 10.3389/fimmu.2018.01093 PMC 바로가기 [Article Type] Immunology
Detection of infectious bronchitis virus 793B, avian metapneumovirus, Mycoplasma gallisepticum and Mycoplasma synoviae in poultry in EthiopiaRegular Articles Published on 2016-12-062022-10-28 Journal: Tropical Animal Health and Production [Category] Coronavirus, MERS, [키워드] aMPV Antibody titre Blood circulating collected Combination conducted detection Ethiopia Ethiopian Genotype highlight IBV Infectious Bronchitis Virus métapneumovirus Mg molecular MS Mycoplasma newcastle disease virus Pathogens PCR Randomly respiratory infectious disease respiratory pathogen selected serological serology strain subset Swab Vaccine variant virulent virus was used [DOI] 10.1007/s11250-016-1195-2 PMC 바로가기 [Article Type] Regular Articles
Evaluation of serological cross-reactivity and cross-neutralization between the United States porcine epidemic diarrhea virus prototype and S-INDEL-variant strainsResearch Article Published on 2016-04-052022-10-28 Journal: BMC veterinary research [Category] Coronavirus, MERS, [키워드] antibody antibody assay Antisera Cell Cell culture collected cross-neutralization cross-reactivity Culture detect detection rate determine diagnostic ELISA epidemic diarrhea evaluated examined FIVE fluorescent generate group IFA in vitro infecting inoculated lab Laboratory medium neutralized offered PEDV Porcine epidemic diarrhea virus positive post inoculation prototype Result S-INDEL serological Serological assay serum sample strain Strains the antibody the United State variant virus Virus neutralization viruses [DOI] 10.1186/s12917-016-0697-5 PMC 바로가기 [Article Type] Research Article
Serological assays for emerging coronaviruses: Challenges and pitfallsArticle Published on 2014-03-232022-11-01 Journal: Virus research [Category] Coronavirus, [키워드] acute respiratory syndrome addition Analysis antibody Antibody detection Antigen applicability applied challenge common cold conserved Contact coronavirus CoV CoV spike protein CoVs cross-reactive antibody cross-reactivity Diagnosis diagnostic diagnostics Epidemic epitope highlight IMPROVE improvement infected individual information Isolation less majority MERS MERS-coronavirus MERS-CoV Middle East multiple method Novel viruses Nucleic acid detection nucleocapsid protein Nucleocapsid proteins occur occurred Occurrence outbreak problem public health authorities reported researcher reservoir reservoirs SARS-coronavirus SARS-CoV Sensitivity and specificity sera serological Serological assay Serological testing serology Spike protein Spread subsequent syndrome tested variety viral antigens [DOI] 10.1016/j.virusres.2014.03.018 PMC 바로가기 [Article Type] Article
Serological report of pandemic (H1N1) 2009 infection among cats in Northeastern China in 2012-02 and 2013-03Short Report Published on 2014-03-142023-06-07 Journal: Virology Journal [Category] 신종인플루엔자, [키워드] cats Pandemic (H1N1) 2009 serological [DOI] 10.1186/1743-422X-11-49 PMC 바로가기 [Article Type] Short Report