SARS-CoV-2 Variant of Concern 202012/01 Has about Twofold Replicative Advantage and Acquires Concerning MutationsSARS-CoV-2 우려 변종 202012/01은 약 2배의 복제 이점을 갖고 돌연변이와 관련하여 획득Article Published on 2021-03-012022-09-10 Journal: Viruses [Category] SARS, 변종, 유전자 메커니즘, 치료기술, [키워드] 20A.EU1 variant B.1.1.7 binding cleavage site collected concern country COVID-19 pandemic Denmark E484K Efficiency England Evolution expand fixation Genome Genome sequencing growth rate growth rates immune escape mutation immune escape mutations initiated L18F Lineage molecular molecular evolution MONITOR mutant Mutation Neutralizing antibodies polybasic Q677H receptor binding motif replicative reported S1/S2 boundary SARS-CoV-2 SARS-CoV-2 genome SARS-CoV-2 genomes sequenced spike L18F Spike protein Spread United Kingdom USA Vaccine VoC VOC-202012/01 [DOI] 10.3390/v13030392 PMC 바로가기 [Article Type] Article
A Negative Feedback Model to Explain Regulation of SARS-CoV-2 Replication and TranscriptionGenetics Published on 2021-02-262022-10-31 Journal: Frontiers in Genetics [Category] COVID-19, [키워드] acute respiratory syndrome caused cleavage site cleaving comprehensive analysis Control coronavirus Coronavirus disease 2019 CoV CoV replication COVID-19 CoVs derivatives drug explain expression level feedback Gene Expression genome sequence genomic RNA hosts influence knowledge mechanism Model molecular basis motif negative Nsp15 persistence proportion Protein regulate regulated Regulation regulation model Replication Result RNA SARS-CoV-2 sgRNA sgRNAs subgenomic RNA Transcription transcription regulatory sequence TRS TRSs turn uridine [DOI] 10.3389/fgene.2021.641445 PMC 바로가기 [Article Type] Genetics
A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entryArticle Published on 2021-02-112022-10-04 Journal: Nature Communications [Category] MERS, 진단, 치료제, [키워드] ACE2 Affect affecting angiotensin-converting enzyme 2 Cell cleavage site clone Coronaviruses CRISPR Critical disrupting endosomal entry pathway entry of SARS-CoV-2 expression Factor genome-wide CRISPR screen highlight Host identify insertion modulating multi-basic cleavage site plasma membrane public health reduced regulate residue Restriction factors S1/S2 S1/S2 boundary S1/S2 site SARS-CoV-2 SARS-CoV-2 entry SARS-CoV-2 spike protein Spread subunit Transmission virus virus entry [DOI] 10.1038/s41467-021-21213-4 PMC 바로가기 [Article Type] Article
The Polybasic Cleavage Site in SARS-CoV-2 Spike Modulates Viral Sensitivity to Type I Interferon and IFITM2Cellular Response to Infection Published on 2021-02-092022-10-30 Journal: Journal of Virology [Category] COVID-19, SARS, [키워드] Activation Antiviral antiviral activity cathepsin Cell cellular entry cleavage cleavage site consequence coronavirus COVID-19 entry of SARS-CoV-2 exacerbated expression furin cleavage furin cleavage site furin-like protease glycoprotein IFITM IFITM2 IFITM3 individuals inhibit inhibited innate immune Innate immunity interferons (IFNs late endosomes maturation membrane protein mitigate polybasic potential therapeutic target precursor producer cell promote protease Protein reduce reduce viral replication Replication restrict S1/S2 boundary SARS-CoV-1 SARS-CoV-1 and -2 SARS-CoV-2 SARS-CoV-2 entry SARS-CoV-2 replication SARS-CoV-2 spike sensitivity Site spike suggested the spike protein therapeutic strategy transmembrane Transmissibility transmissibility of SARS-CoV-2 Transmission Treatment Type type 1 interferon type I type I IFNs type I interferon type II IFN viral entry viral spike viruses [DOI] 10.1128/JVI.02422-20 PMC 바로가기 [Article Type] Cellular Response to Infection
Development of a Cell-Based Luciferase Complementation Assay for Identification of SARS-CoV-2 3CL pro InhibitorsArticle Published on 2021-01-242022-10-28 Journal: Viruses [Category] COVID-19, [키워드] 3CL pro 3CLpro Antiviral antiviral drug BSL-2 BSL-3 facility Cell cleavage cleavage site Complementation compounds COVID-19 current cytotoxicity development effort exhibited facilitate False positive FIVE HCV HIV identification identify in vitro inhibit SARS-CoV-2 inhibitor inhibitors of SARS-CoV-2 lack lentiviral limitations luciferase luciferase activity other compound performed protease protease inhibitors Proteases reduce replication-competent virus reported reporter SARS-CoV-2 screened small molecule specificity viral replication Z-FA-FMK [DOI] 10.3390/v13020173 PMC 바로가기 [Article Type] Article
SARS-CoV-2 variants with mutations at the S1/S2 cleavage site are generated in vitro during propagation in TMPRSS2-deficient cellsS1/S2 절단 부위에 돌연변이가 있는 SARS-CoV-2 변이체는 TMPRSS2 결핍 세포에서 증식하는 동안 시험관 내에서 생성됩니다Research Article Published on 2021-01-212022-08-13 Journal: PLoS Pathogens [Category] Biochemistry, COVID-19, [키워드] alteration antivirals bind Cell cell lines cell tropism cleavage cleavage site cleaved Coronaviruses Critical de novo demonstrated direct fusion dominant entry pathway exhibited experiments facilitate generate Host host cell host cell receptor host protease in vitro inefficient infect motif mutant mutants Mutation performed plaque plaques polybasic Protein proteolytic cleavage researcher respiratory S gene S protein S1/S2 cleavage site SARS-CoV-2 SARS-CoV-2 entry SARS-CoV-2 propagation SARS-CoV-2 S gene SARS-CoV-2 variant SARS-CoV-2 variants screened Serine Severe acute respiratory syndrome severe acute respiratory syndrome-coronavirus-2 shown target cells the cell the S protein TMPRSS2 transmembrane serine protease Transmissibility type strain undergo Vaccine variant Vero Cell Vero cells Viral viral entry virus virus strains virus variants viruses wild-type strain [DOI] 10.1371/journal.ppat.1009233 PMC 바로가기 [Article Type] Research Article
Structure of the multiple functional domains from coronavirus nonstructural protein 3Coronaviruses Published on 2021-01-172022-10-31 Journal: Emerging Microbes & Infections [Category] COVID-19, [키워드] active site activity Antiviral asymmetric unit catalytic domain cleavage site cleave complex conducted conserved coronavirus CoV CoVs domain drug design enzyme activity exhibit fluorescent functional homodimer Host infect Interaction macro Macro domain mimicking monomer motif Multiple functional domains mutant Neurological diseases nonstructural protein 3 Nsp3 pandemic Papain-like protease papain-like protein pathogen peptide PLPro protease protein 3 replication mechanism significantly stability stimulated Structure substrate substrates subunit targets ubiquitin-like domain variety [DOI] 10.1080/22221751.2020.1865840 PMC 바로가기 [Article Type] Coronaviruses
Free SARS-CoV-2 Spike Protein S1 Particles May Play a Role in the Pathogenesis of COVID-19 Infection무료 SARS-CoV-2 스파이크 단백질 S1 입자는 COVID-19 감염의 병인에서 역할을 할 수 있습니다Hypothesis Published on 2020-12-302022-09-01 Journal: Biochemistry. Biokhimiia [Category] SARS, 변종, 진단, [키워드] accompanied ACE ACE2 angiotensin angiotensin (1-7) Angiotensin II Angiotensin-converting enzyme angiotensin-converting enzyme 2 association caused Cell cell surface circulation cleavage site COVID-19 COVID-19 disease COVID-19 infection COVID-19 pathogenesis D614G D614G mutation decrease disease downregulate downregulated Effect expression fraction Free furin furin protease higher infectivity Hypothesis Imbalance in vivo infected cell infected cells Infection Inflammation investigated lack lead less lung Lungs membrane-bound murine model mutant natural infection Particle Pathogenesis pathogenesis of COVID-19 Prevalence Protein pulmonary damage receptor-binding domains Recombinant spike protein Renin renin-angiotensin system reported role S protein S1 and S2 S1 subunit S1 subunit shedding SARS-CoV virus SARS-CoV-2 SARS-CoV-2 S protein SARS-CoV-2 strain separation severity shown spike Spike protein subunits the cell the receptor-binding domain the S protein the S1 subunit the SARS-CoV-2 thrombosis trimer unlikely Viral Viral load virion virions wild type [DOI] 10.1134/S0006297921030032 PMC 바로가기 [Article Type] Hypothesis
Design, Expression, Purification, and Characterization of a YFP-Tagged 2019-nCoV Spike Receptor-Binding Domain ConstructBioengineering and Biotechnology Published on 2020-12-212022-10-31 Journal: Frontiers in Bioengineering and Biotechnology [Category] COVID-19, [키워드] 2019-nCoV 2019-nCoV spike protein 2019-nCoV spike-RBD production using standard cell culture equipment and techniques ACE2 adherent cell Analysis Antigen assays binding C-terminal Cell cells characterization cleavage site coronavirus disease COVID-19 disulfide bond domain experiment expression FLAG-tag fluorescent fluorescent spike RBD fusion protein fusion protein human Angiotensin-converting enzyme Human rhinovirus IFNα2 immunization in vitro include Interferon alpha labeling mammalian protein secretion Microscopy N-linked glycosylation N-terminal Ni-NTA pandemic produced protease Protein protein reagent for COVID-19 research proteolytic cleavage protocol purification RBD receptor required Research SDS-PAGE SEC secreted secreted glycosylated YFP-labeled protein secreted protein secretion serum-free medium signal peptide spike staining the RBD Vaccine virus YFP-spike_RBD [DOI] 10.3389/fbioe.2020.618615 PMC 바로가기 [Article Type] Bioengineering and Biotechnology
Identification of Host Cellular Protein Substrates of SARS-COV-2 Main ProteaseSARS-COV-2 주요 프로테아제의 숙주 세포 단백질 기질의 확인Article Published on 2020-12-152022-09-10 Journal: International Journal of Molecular Sciences [Category] MERS, SARS, 유전자 메커니즘, [키워드] 3CL Protease 3CLpro acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Algorithms Analysis causative agent cellular cleavage cleavage site cleavage site identification cleavage site prediction cleavage sites cleaved contribute coronavirus coronavirus disease Coronavirus disease-19 COVID-19 CTBP1 develop help highlight homologous Host host cell host protein cleavage Human human protein human proteins identification In silico method in vitro Interaction knowledge Like limitation main protease molecular mechanism molecular mechanisms NetCorona Pathogenesis pathogenesis of SARS-CoV-2 polyprotein predict predicted processed protease Protein protein sequence Replication respiratory SARS SARS-CoV SARS-CoV-2 sequence severe pneumonia SSHHPS substrate target tested Viral viruses was used [DOI] 10.3390/ijms21249523 PMC 바로가기 [Article Type] Article