Ontology-Enabled Emotional Sentiment Analysis on COVID-19 Pandemic-Related Twitter Streams코로나19 팬데믹 관련 트위터 스트림에 대한 온톨로지 지원 감성 감정 분석Public Health Published on 2021-12-062022-09-11 Journal: Frontiers in Public Health [Category] MERS, SARS, 치료기술, [키워드] analyzed approach approaches carried changed COVID-19 detect emotion ontology emotional exponential growth extraction false negatives growth hidden information latent Dirichlet allocation mechanism media natural language processing paradigm performed provide second sentiment analysis social media SPARQL stream true positive true positives twitter streams user-generated content [DOI] 10.3389/fpubh.2021.798905 PMC 바로가기 [Article Type] Public Health
Impact of the Nucleic Acid Extraction Method and the RT-qPCR Assay on SARS-CoV-2 Detection in Low-Viral SamplesArticle Published on 2021-11-302022-10-31 Journal: Diagnostics [Category] COVID-19, [키워드] acid analyzed China clinical sample COVID-19 COVID-19 case deaths demonstrated detect detection diagnostic Diagnostic method disease evaluated extraction gold standard Impact Laboratory MONITOR Novel coronavirus pandemic reported RNA extraction RNA extraction method RNA loads RT-qPCR RT-qPCR assay Sample SARS-CoV-2 selected specimen Spread the SARS-CoV-2 the WHO upper respiratory tract viral loads virus were recorded [DOI] 10.3390/diagnostics11122247 PMC 바로가기 [Article Type] Article
Extraction, Isolation and Characterization of Bioactive Compounds from Artemisia and Their Biological Significance: A Review아르템이시아에서 생리활성 화합물의 추출, 분리 및 특성화와 그 생물학적 중요성: 리뷰Review Published on 2021-11-192024-08-08 Journal: Molecules [Category] 말라리아, [키워드] Artemisia bioactive compounds biological activity extraction Isolation [DOI] 10.3390/molecules26226995 PMC 바로가기 [Article Type] Review
SARS-CoV-2 detection using reverse transcription strand invasion based amplification and a portable compact size instrument역전사 가닥 침습 기반 증폭 및 휴대용 소형 크기 기기를 사용한 SARS-COV-2 검출Comparative Study Published on 2021-11-152022-09-01 Journal: Scientific Reports [Category] MERS, SARS, 바이오마커, 진단, 치료기술, [키워드] amplification analytical sensitivity automatically clinical sensitivity closed system comparable Containment extraction help Integrated optics Invasion isothermal isothermal amplification Laboratory lockdowns molecular nucleic acid nucleic acid extraction oropharyngeal swab pandemic PCR performed Prevent professionals Rapid reaction time resulting reverse transcription SARS-CoV-2 SARS-CoV-2 detection SARS-CoV-2 virus sealed specificity System Viral viral infection Viral RNA widespread [DOI] 10.1038/s41598-021-01744-y PMC 바로가기 [Article Type] Comparative Study
Fighting SARS-CoV-2 with green seaweed Ulva sp. extract: extraction protocol predetermines crude ulvan extract anti-SARS-CoV-2 inhibition properties in in vitro Vero-E6 cells assayBioengineering Published on 2021-11-152022-10-28 Journal: PeerJ [Category] COVID-19, [키워드] Ulva sp. Ammonium analyzed anti-SARS-CoV-2 Antiviral antiviral activity antivirals average Bioactivity Cell Compound COVID-19 pandemic Cytopathic effect cytotoxicity extraction Fighting in vitro macroalgae molecular weight protocol protocols reduction SARS-CoV-2 seaweed sulfated polysaccharides Ulva Ulvan VERO E6 cells VERO-E6 [DOI] 10.7717/peerj.12398 PMC 바로가기 [Article Type] Bioengineering
Disparities in COVID-19 Outcomes by Race, Ethnicity, and Socioeconomic Status A Systematic-Review and Meta-analysisResearch Published on 2021-11-112022-10-29 Journal: JAMA Network Open [Category] COVID-19, MERS, [키워드] 95% CI adjusted Admission affected African American age Alaska analyses Asian association clinical care COVID-19 criteria database Decreased Deprivation determinant determinants disease severity disparity Ethnicity evaluate extraction finding group groups higher risk highest Hispanic ICU admission Importance incidence independent individual individuals intensive care item less lower mortality measure Meta-analysis Mortality mortality rate Native objective Odds ratio outcome outcomes Patient Point populations positive positively correlated Prevalence public health quality score race Racial random-effects model Relevance reported Reporting Result review risk risk ratio RRs screened Selection Source status synthesis systematic review the United State was performed were assessed were excluded white with COVID-19 World Health Organization [DOI] 10.1001/jamanetworkopen.2021.34147 PMC 바로가기 [Article Type] Research
Vitality of Proteinase K in rRTPCR Detection of SARS-CoV2 Bypassing RNA ExtractionSARS-CoV2 우회 RNA 추출의 rRTPCR 검출에서 Proteinase K의 활력Cellular and Infection Microbiology Published on 2021-11-032022-09-11 Journal: Frontiers in Cellular and Infection Microbiology [Category] MERS, SARS, 치료기술, [키워드] acid Chain Reaction COVID – 19 demonstrated detect detection extraction followed by group heat inactivation inoculated molecular negative sample nucleic acid nucleic acids ORF1 ORF1ab gene polymerase chain polymerase chain reaction positive processed Proteinase K reagent real time PCR real-time polymerase chain reaction RNA RNA extraction SARS-CoV-2 detection SARS-CoV2 sensitivity temperature Template tested three groups treated Treatment Viral vitality VTM were used [DOI] 10.3389/fcimb.2021.717068 PMC 바로가기 [Article Type] Cellular and Infection Microbiology
Evaluation of extraction-free RT-PCR methods for faster and cheaper detection of SARS-CoV-2 using two commercial systems두 가지 상용 시스템을 사용하여 SARS-CoV-2를 더 빠르고 저렴하게 검출하기 위한 추출 없는 RT-PCR 방법 평가Article Published on 2021-11-012022-09-11 Journal: International Journal of Infectious Diseases [Category] MERS, SARS, 진단, 치료기술, [키워드] analytical sensitivity approach avoidance Chain Reaction Ct values diagnostic diagnostic platform Dilution evaluated exhibited extraction extraction-free method False negative false negative result faster free water heat exposure help IMPROVE Including initial molecular molecular diagnostics MOST Nasopharyngeal swab nasopharyngeal swabs NPs nucleic acid nucleic acid extraction pandemic platform polymerase chain polymerase chain reaction protocol protocols reagent reagents real-time polymerase chain reaction reduce reducing Result RT-PCR SARS-CoV-2 SARS-CoV-2 detection specimen standard RNA step tested Viral Viral load water [DOI] 10.1016/j.ijid.2021.09.046 PMC 바로가기 [Article Type] Article
Interlaboratory comparison of SARS-CoV2 molecular detection assays in use by U.S. veterinary diagnostic laboratories미국 수의 진단 연구소에서 사용 중인 SARS-CoV2 분자 검출 분석의 연구소 간 비교Article Published on 2021-11-012022-09-11 Journal: Journal of veterinary diagnostic investigation : o [Category] SARS, 치료기술, [키워드] affected analyzed buffer CDC assay CDC assays clinical sample clinical samples collaboration Critical Ct values diagnostic evaluate evaluated expected extraction Factors help Host indicated interlaboratory comparison Laboratory limit of detection medium molecular Multiplex PCR National organization organizations outbreak PCR reaction Probability protocols Qualitative Quantitative quantitative assessment reaction real-time RT-PCR reported reproducibility reservoir Result RNA RNA sample RT-PCR assay sample collection SARS-CoV2 SARS-CoV2 detection sensitivity Standard deviation Standardization Surveillance TE buffer Transport transport medium tris was performed [DOI] 10.1177/10406387211029913 PMC 바로가기 [Article Type] Article
Robust Saliva-Based RNA Extraction-Free One-Step Nucleic Acid Amplification Test for Mass SARS-CoV-2 Monitoring대량 SARS-CoV-2 모니터링을 위한 강력한 타액 기반 RNA 추출 없는 원스텝 핵산 증폭 테스트Article Published on 2021-10-312022-09-10 Journal: Molecules [Category] MERS, SARS, 치료기술, [키워드] acid analyzed Asymptomatic buffer Chain Reaction Compliance COVID-19 detect Diagnosis diagnostic sensitivity early diagnosis extraction extraction-free RT-qPCR form help IMPROVE individual Infection isothermal amplification LAMP Loop-mediated isothermal amplification mass monitoring nasal Nasopharyngeal swab oral cavity oral cavity swab passive drool Patient polymerase chain polymerase chain reaction pooling populations qPCR quantitative polymerase chain reaction repeated reverse transcription RNA RNA extraction RT-LAMP RT-qPCR RT-qPCR test Saliva saliva specimen SARS-CoV-2 SARS-COV-2 infection screening program sensitivity specimen Spread subclinical infection Swab swab specimen symptomatic individuals Test The diagnostic sensitivity Viral viral spread virus was collected [DOI] 10.3390/molecules26216617 PMC 바로가기 [Article Type] Article