Structural Insight into the Binding of Cyanovirin-N with the Spike Glycoprotein, M pro and PL pro of SARS-CoV-2: Protein–Protein Interactions, Dynamics Simulations and Free Energy CalculationsSARS-CoV-2의 스파이크 당단백질, M pro 및 PL pro와 시아노비린-N의 결합에 대한 구조적 통찰력: 단백질-단백질 상호작용, 역학 시뮬레이션 및 자유 에너지 계산Article Published on 2021-08-242022-09-10 Journal: Molecules [Category] MERS, 치료제, [키워드] Antiviral approach attachment binding binding affinities binding energy calculated calculation claimed contributed COVID-19 cyanobacteria cyanovirin-N Dynamics energy Evidence form Free global public health highest human ACE2 receptor in vitro in vivo experiment insight Interaction investigated involved M pro MPro molecular molecular docking molecular dynamics simulations Molecular mechanics offer pandemic phycocyanin PLpro polar protease Protein Proteins Replication replication of SARS-CoV-2 residue SARS-CoV-2 scytovirin Simulation Spike protein surface area the binding affinity the Spike the spike protein threat Viral Viral proteins [DOI] 10.3390/molecules26175114 PMC 바로가기 [Article Type] Article
DNA Aptamers Block the Receptor Binding Domain at the Spike Protein of SARS-CoV-2Molecular Biosciences Published on 2021-08-122022-10-31 Journal: Frontiers in Molecular Biosciences [Category] COVID-19, [키워드] ACE2 ACE2 receptor affected Angiotensin converting enzyme-2 aptamer binding binding site block cell receptors conditions curative develop DNA DNA aptamers effective Evidence free energies genetic material host cell host cell membrane human ACE2 receptor human cells inaccessible Interaction membrane molecular molecular dynamics molecular simulations nucleotide Probability Proteins recognize recognizing reducing S-protein S1 domain SARS-CoV-2 sensitivity significantly single-stranded Spike protein subsequent the SARS-CoV-2 the Spike trimer virus [DOI] 10.3389/fmolb.2021.713003 PMC 바로가기 [Article Type] Molecular Biosciences
Modelling conformational state dynamics and its role on infection for SARS-CoV-2 Spike protein variants모델링 형태 상태 역학 및 SARS-COV-2 스파이크 단백질 변이체에 대한 감염에 대한 역할Research Article Published on 2021-08-052022-08-13 Journal: PLoS Computational Biology [Category] MERS, SARS, 변종, 유전자 메커니즘, [키워드] 501.V2 ACE2 alternative methods B.1.1.7 calculate cell entry computational method conformational COVID-19 D614G decrease facilitate flexibility glycine helping human ACE2 receptor identify increase in Infection K417 knowledge less matter mechanism molecular mechanism molecular mechanisms multiple mutation multiple mutations Mutation N501Y N501Y mutation new strain normal mode analysis open Pandemics predict proof proof of concept Protein public health residue residues resource SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 spike protein several variant several variants Simulation specific mutation specific mutations spike Spike protein Strains Surveillance the SARS-CoV-2 the Spike transition probabilities transition probability treat variant variants Viral viral entry virus [DOI] 10.1371/journal.pcbi.1009286 PMC 바로가기 [Article Type] Research Article
Computational design of SARS-CoV-2 peptide binders with better predicted binding affinities than human ACE2 receptorArticle Published on 2021-08-022022-10-04 Journal: Scientific Reports [Category] MERS, SARS, 변종, [키워드] ACE2 angiotensin-converting enzyme 2 approach bind binding binding affinity candidate coronavirus COVID-19 COVID-19 pandemic domain human ACE2 receptor human cells inhibit inhibitors Interaction molecular dynamics Mutation peptide peptides predicted Protein design receptor Receptor binding domain reported residue S1 subunit SARS-CoV-2 SARS-CoV-2-RBD single mutation stability suggested therapeutic α1 helix [DOI] 10.1038/s41598-021-94873-3 PMC 바로가기 [Article Type] Article
Combination of a Sindbis-SARS-CoV-2 Spike Vaccine and αOX40 Antibody Elicits Protective Immunity Against SARS-CoV-2 Induced Disease and Potentiates Long-Term SARS-CoV-2-Specific Humoral and T-Cell Immunitysindbis-sars-cov-2 스파이크 백신과 αOX40 항체의 조합은 SARS-COV-2 유도 질환에 대한 보호 면역을 유도하고 장기 SARS-COV-2- 특이 적 유머 및 T- 세포 면역을 강화시킨다.Immunology Published on 2021-07-292022-08-31 Journal: Frontiers in Immunology [Category] SARS, 바이오마커, 변종, 진단, [키워드] αOX40 activated T cells Against alphavirus vaccine Analysis antibody approach can be used caused cellular coronavirus coronavirus SARS-CoV-2 COVID-19 pandemic COVID19 develop dose effective effective vaccine effector memory effort elicit elicited expressing functions generate hACE2 human ACE2 receptor immune protection immune response immunization immunize Immunophenotyping immunostimulatory induced Infection infection assay inhibit long-lasting neutralizing antibodies long-lasting neutralizing antibody lung tissue mice MOST mounted Neutralizing antibodies OX40 Pathogens platform profiles protective immunity Protein binding provided reprogramming RNA robust SARS-CoV-2 SARS-CoV-2 immunity SARS-CoV-2 spike protein SARS-CoV-2 vaccine sera Sindbis virus vaccine spike Spike protein synergistic combination SARS-CoV-2 vaccine strategy T cell T cells T-cell Response T-cell responses the SARS-CoV-2 the vaccine Transcriptome transgenic mice Vaccine vaccine platform variant variants [DOI] 10.3389/fimmu.2021.719077 PMC 바로가기 [Article Type] Immunology
Implementation of Adenovirus-Mediated Pulmonary Expression of Human ACE2 in HLA Transgenic Mice Enables Establishment of a COVID-19 Murine Model for Assessment of Immune Responses to SARS-CoV-2 InfectionCommunication Published on 2021-07-262022-10-28 Journal: Pathogens [Category] COVID-19, [키워드] ACE2 Adenovirus Vector animal model anti-viral immune responses assessment Cellular immune response COVID-19 days post-infection develop Efficacy Evolution expression full recovery HLA HLA transgenic mice Human human ACE2 human ACE2 receptor humoral immune response immunized mice implementation Infection infection with SARS-CoV-2 mice Model Post-infection Protective protocol pulmonary Re-infection reproducibility response SARS-CoV-2 SARS-COV-2 infection spike-protein strain subsequent the disease transgenic mice Transient Variability viral infection virus [DOI] 10.3390/pathogens10080940 PMC 바로가기 [Article Type] Communication
Structural Modeling of the SARS-CoV-2 Spike/Human ACE2 Complex Interface can Identify High-Affinity Variants Associated with Increased TransmissibilitySARS-CoV-2 스파이크/인간 ACE2 복합 인터페이스의 구조 모델링은 전파율 증가와 관련된 고친화도 변이를 식별할 수 있습니다.Article Published on 2021-07-232022-09-11 Journal: Journal of Molecular Biology [Category] SARS, 변종, [키워드] ACE2 affinity Analysis antibody antibody resistance Applying binding binding affinities binding affinity changes in circulating Cluster Combination combinatorial mutations complex complexes COVID-19 pandemic determine E484K Effect flexible functional G476S generate hACE2 help human ACE2 human ACE2 receptor Increased Infection Interaction interface Intervention K31 K353 modeling mutant Mutation N501Y pathogenic Point mutation predict predicted Protein Protein binding public health measure Region reveal S477N SARS-CoV-2 SARS-CoV-2 spike protein screening method sequence spike spike mutations Spike protein Structural analysis structural modeling susceptibility susceptibility to infection the SARS-CoV-2 Transmissibility transmissible variants transmissible variants. triggered variant variants Variation viral strains viral variant wild-type [DOI] 10.1016/j.jmb.2021.167051 PMC 바로가기 [Article Type] Article
Antiviral effects of probiotic metabolites on COVID-19COVID-19에 대한 프로바이오틱 대사물의 항바이러스 효과Article Published on 2021-07-012022-09-11 Journal: Journal of biomolecular structure & dynamics [Category] SARS, 치료제, [키워드] ACE2 ACE2 receptor protein angiotensin Angiotensin-converting enzyme binding binding energy catalytic site complexes COVID-19 Docking studies docking study Effect effort enzyme Epidemic Health host cells Human human ACE2 receptor inhibiting Interaction mechanism metabolite metabolites molecular dynamics other compounds pathogenic plantaricin polymerase Probiotics Protein RBD RdRP receptor protein residue residues RNA RNA dependent RNA polymerase RNA-dependent RNA polymerase S protein S proteins SARS Coronavirus SARS-CoV-2 RdRp Spike protein Spike proteins stability Treatment virus [DOI] 10.1080/07391102.2020.1775123 PMC 바로가기 [Article Type] Article
Attractive and repulsive residue fragments at the interface of SARS-CoV-2 and hACE2Article Published on 2021-06-152022-10-29 Journal: Scientific Reports [Category] COVID-19, [키워드] addition antibody binding binding affinity Biophysics conformation Contact coronavirus cryo-EM Epitopes hACE2 human ACE2 receptor human cell identify include indicate initial Interaction intrinsically involved mechanism Microscopy molecular biophysics Molecular modelling Other promote Protein Receptor binding domain residue S-RBD SARS-CoV-1 SARS-CoV-2 SARS-CoV-2 coronavirus SARS-CoV-2 spike protein Stage structural biology the SARS-CoV-2 van der Waal viral spike Virology X-ray crystallography [DOI] 10.1038/s41598-021-91877-x PMC 바로가기 [Article Type] Article
Expression of Human ACE2 N-terminal Domain, Part of the Receptor for SARS-CoV-2, in Fusion With Maltose-Binding Protein, E. coli Ribonuclease I and Human RNase AMicrobiology Published on 2021-06-112022-10-31 Journal: Frontiers in Microbiology [Category] COVID-19, [키워드] E. coli ribonuclease I (RNase I) ACE2 ACE2 protein Antiviral antiviral agent bacterial cell Cartoon cleaving degrade dsRNA E. coli enzyme evaluated expressed expression fusion fusion protein Genome Human human ACE2 receptor human RNase A-ACE2NTD fusion in vitro Inclusion Infection Membrane proteins N-terminal N-terminal domain Nucleocapsid proteins Part Protein Proteins provide purified receptor ribonuclease RNA RNA degradation RNase RNase I-ACE2NTD fusion RNase III RNases SARS-CoV-2 SARS-CoV-2 RNA SARS-CoV-2 viral SARS-CoV-2 virus single-stranded soluble fraction Strains viral genome viral nucleic acids virions [DOI] 10.3389/fmicb.2021.660149 PMC 바로가기 [Article Type] Microbiology