COVID-19 lung CT image segmentation using deep learning methods: U-Net versus SegNet딥 러닝 방법을 사용한 코로나19 폐 CT 영상 분할: U-Net 대 SegNetTechnical Advance Published on 2021-02-092022-09-01 Journal: BMC Medical Imaging [Category] Fulltext, MERS, 진단, [키워드] Accuracy assist calculated characterized Computerized tomography COVID-19 COVID-19 patient COVID-19 patients CT scan deep learning diagnosis of COVID-19 Disease diagnosis feasible healthy lung tissue help Image segmentation Infection investigated labeling lung Lung CT Lung CT scans pandemic patients Pneumonia Region Result SegNet Semantic segmentation severity statistical tested tissue Treatment U-NET [DOI] 10.1186/s12880-020-00529-5 PMC 바로가기 [Article Type] Technical Advance
A Ligand Selection Strategy Identifies Chemical Probes Targeting the Proteases of SARS‐CoV‐2Research Article Published on 2021-01-282022-10-28 Journal: Angewandte Chemie (International ed. in English) [Category] COVID-19, SARS, [키워드] 3CL pro ABPP approach Cell Chemical chemical probes complementary cysteine protease derivative develop Efficacy electrophilic enzyme Enzyme inhibitors inhibitor inhibitors labeling Ligand probe protease Proteases proteome proteomic resulting SARS‐CoV‐2 Selection specificity Strategy target protein targeting tuning virus [DOI] 10.1002/anie.202016113 PMC 바로가기 [Article Type] Research Article
Design, Expression, Purification, and Characterization of a YFP-Tagged 2019-nCoV Spike Receptor-Binding Domain ConstructBioengineering and Biotechnology Published on 2020-12-212022-10-31 Journal: Frontiers in Bioengineering and Biotechnology [Category] COVID-19, [키워드] 2019-nCoV 2019-nCoV spike protein 2019-nCoV spike-RBD production using standard cell culture equipment and techniques ACE2 adherent cell Analysis Antigen assays binding C-terminal Cell cells characterization cleavage site coronavirus disease COVID-19 disulfide bond domain experiment expression FLAG-tag fluorescent fluorescent spike RBD fusion protein fusion protein human Angiotensin-converting enzyme Human rhinovirus IFNα2 immunization in vitro include Interferon alpha labeling mammalian protein secretion Microscopy N-linked glycosylation N-terminal Ni-NTA pandemic produced protease Protein protein reagent for COVID-19 research proteolytic cleavage protocol purification RBD receptor required Research SDS-PAGE SEC secreted secreted glycosylated YFP-labeled protein secreted protein secretion serum-free medium signal peptide spike staining the RBD Vaccine virus YFP-spike_RBD [DOI] 10.3389/fbioe.2020.618615 PMC 바로가기 [Article Type] Bioengineering and Biotechnology
Foundation and Clinical Evaluation of a New Method for Detecting SARS-CoV-2 Antigen by Fluorescent Microsphere Immunochromatography형광 미소구체 면역크로마토그래피에 의한 SARS-CoV-2 항원 검출을 위한 새로운 방법의 기초 및 임상 평가Cellular and Infection Microbiology Published on 2020-11-302022-09-11 Journal: Frontiers in Cellular and Infection Microbiology [Category] MERS, SARS, 진단, 치료기술, 치료법, [키워드] activated Analytical specificity antibody Antigen antigen detection auxiliary clinical clinical evaluation coronary pneumonia COVID-19 COVID-19 case cured cut-off value develop Diagnosis evaluated fluorescent foundation healthy control healthy controls immunochromatographic immunochromatographic test labeling limit of detection methodological microsphere N protein N-protein New Patient Pneumonia positive principle reagent respiratory Respiratory disease Result RNA SARS-CoV-2 SARS-CoV-2 antigen sensitivity specificity spot TCID test strip virus viruses [DOI] 10.3389/fcimb.2020.553837 PMC 바로가기 [Article Type] Cellular and Infection Microbiology
Field-Effect Sensors for Virus Detection: From Ebola to SARS-CoV-2 and Plant Viral EnhancersPlant Science Published on 2020-11-242022-10-31 Journal: Frontiers in Plant Science [Category] COVID-19, [키워드] acute respiratory syndrome adapter antibody applied biosensor charged biomolecules clinical sample coronavirus Coronavirus disease 2019 COVID-19 demonstrated diagnostic test drug early diagnosis Ebola electrical element enzyme explained faster field effect functional highest human infectious disease humans intrinsic Label-free detection labeling outbreak Perspective plant plant virus nanoparticle plant VLP tool platform point-of-care Protein subunit receptor repeated SARS-CoV-2 sensor the disease time-consuming Treatment Vaccine Viral diseases Viral particles virus virus detection virus spread viruses [DOI] 10.3389/fpls.2020.598103 PMC 바로가기 [Article Type] Plant Science
Toward Preparing a Knowledge Base to Explore Potential Drugs and Biomedical Entities Related to COVID-19: Automated Computational ApproachOriginal Paper Published on 2020-11-102022-10-31 Journal: JMIR Medical Informatics [Category] COVID-19, [키워드] 2019-nCoV Analysis applicability applied approach association automated base clinical status Community computational method confidence score coronavirus coronavirus disease COVID-19 deep learning Dexamethasone disease Diseases drug drug side effects drugs effective effective drug Effectiveness effort entity Evidence Genes help highlight human genes human miRNAs Hydroxychloroquine IMPROVE information Interaction Ivermectin labeling lower mortality novel objective patients hospitalized pharmacological treatment platform Potential Preparing public health Remdesivir Research resources Result resulting SARS SARS-CoV-2 Scientific community similarity statin statins Therapeutic treatment Treatment Vaccine with COVID-19 [DOI] 10.2196/21648 PMC 바로가기 [Article Type] Original Paper
Comprehensive assessment of side effects in COVID-19 drug pipeline from a network perspectiveResearch article Published on 2020-11-012022-10-05 Journal: Food and chemical toxicology : an international jo [Category] SARS, 신약개발, 치료제, [키워드] adverse event Analysis antiviral therapeutics approach approaches association clinical evidence Clinical practice clinical trials CMap Comprehensive coronavirus disease Course COVID-19 COVID-19 drug drug drugs FDA food gene-set enrichment analysis global public health GSEA human tissue identify labeling lung Network proximity occurred OpenVigil patients with COVID-19 performed Perspective pharmacovigilance provide renal Safe safety profile SARS-CoV-2 Side effect therapeutic agent tissues Toxicity Treatment [DOI] 10.1016/j.fct.2020.111767 [Article Type] Research article
High-throughput viral microneutralization method for feline coronavirus using image cytometryArticle Published on 2020-09-232022-11-01 Journal: Journal of virological methods [Category] Coronavirus, MERS, [키워드] absence accelerate addition antibodies antigenicity Antiviral compounds antiviral drug approved assays Asymptomatic automatically buffer Celigo Cell characterized clinical conditions Concentration coronavirus Coronavirus infection cytolytic cytometer Cytometry death Effects faster fatal disease Feline coronavirus (FCoV) fluorescent generate high-throughput screening (HTS) Image Image cytometry immune correlate immune response Infection labeling majority microneutralization mutate performed plaque reduction neutralization Plaque-Reduction neutralization tests (PRNT) plaques plasma populations Prevent PRNT Protein provide quantification reducing robust sensitivity serotype therapeutic time-consuming treat type I Vaccine vaccine candidate Vaccines Variation viral burden viral infectious disease viral neutralization viral titer virus virus-infected cell was used wash buffer [DOI] 10.1016/j.jviromet.2020.113979 PMC 바로가기 [Article Type] Article
Constructing and Communicating COVID-19 Stigma on Twitter: A Content Analysis of Tweets during the Early Stage of the COVID-19 OutbreakArticle Published on 2020-09-192022-10-29 Journal: International Journal of Environmental Research an [Category] COVID-19, SARS, [키워드] coding Communicating content content analysis Coronavirus 2019 COVID-19 early stage engage include information labeling lack less model of stigma communication outbreak public health Public Health Agency Randomly Result selected Stage Stigma Twitter unique urgency [DOI] 10.3390/ijerph17186847 PMC 바로가기 [Article Type] Article
Signal amplification by reversible exchange for COVID-19 antiviral drug candidatesArticle Published on 2020-08-312022-10-04 Journal: Scientific Reports [Category] MERS, 치료법, 치료제, [키워드] amplification Analytical chemistry antiviral drug candidate carried Clinical treatment Coronavirus pneumonia COVID-19 dependent on Diagnosis disease drug drug candidate Favipiravir feature Hydroxychloroquine labeling Medical and clinical diagnostics molecular NMR spectroscopy nuclear nuclei Perspective Research signal structures tested therapeutic transfer Treatment Trial [DOI] 10.1038/s41598-020-71282-6 PMC 바로가기 [Article Type] Article