Plant Molecular Farming: A Viable Platform for Recombinant Biopharmaceutical ProductionReview Published on 2020-07-042022-10-28 Journal: Plants [Category] COVID-19, [키워드] Nicotiana antigens Bacteria biopharmaceutical biopharmaceuticals cultures expression Factor Genetic mammalian cell molecular molecular farming monoclonal antibodies plant plant production system plant-derived protein plants platform produced Production Protein recombinant Recombinant protein reduced significantly transient expression Vaccine Viable [DOI] 10.3390/plants9070842 PMC 바로가기 [Article Type] Review
Development of a Portable, Ultra-Rapid and Ultra-Sensitive Cell-Based Biosensor for the Direct Detection of the SARS-CoV-2 S1 Spike Protein AntigenLetter Published on 2020-05-312022-10-29 Journal: Sensors (Basel, Switzerland) [Category] COVID-19, MERS, SARS, [키워드] addition antibody Antigen applied Asymptomatic Bioelectric Recognition Assay (BERA) biosensor cellular chimeric coronavirus COVID-19 pandemic cross-reactivity detect detection Detection limit development Direct expressed individuals mammalian cell membrane engineering membrane-bound nucleocapsid protein number of infected pandemic patients platform Point-of-Care (POC) Protein provided Recognition S1 spike protein SARS-CoV-2 selective Serological assay severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) spike Spike protein the SARS-CoV-2 virus [DOI] 10.3390/s20113121 PMC 바로가기 [Article Type] Letter
Controlled branched-chain amino acids auxotrophy in Listeria monocytogenes allows isoleucine to serve as a host signal and virulence effectorListeria monocytogenes의 조절된 분지쇄 아미노산 영양요구성은 이소류신이 숙주 신호 및 독성 효과기 역할을 할 수 있도록 합니다.Research Article Published on 2018-03-122022-09-06 Journal: PLoS Genetics [Category] Communicable Disease, [키워드] activate adaptation amino acids Bacteria Bacterial pathogens BCAA BCAA biosynthesis BCAA biosynthesis genes branched-chain amino acid branched-chain amino acids CodY conditions Control Controlled deficiency dependent on downstream gene downstream genes Evidence expression function Gene regulation highlight Host Infection intracellular pathogen isoleucine L . monocytogene Listeria Listeria monocytogene Listeria monocytogenes mammalian cell mechanism metabolic pathway metabolic pathways metabolism metabolites occur pathogen pathogenic Pathogens regulate Regulation represse respond restrict Rli60 saprophyte switching transcribed transition Trigger triggers upregulated upstream virulence virulence gene virulence genes virulent while [DOI] 10.1371/journal.pgen.1007283 PMC 바로가기 [Article Type] Research Article
Infectious Bronchitis Virus Infection Induces Apoptosis during Replication in Chicken Macrophage HD11 CellsArticle Published on 2017-07-262022-10-28 Journal: Viruses [Category] Coronavirus, [키워드] Activation Ammonium Analysis Apoptosis apoptosis induction apoptotic blocked bronchitis caspase Caspases caused Cell cells cellular chicken chicken macrophage chloride CPE Cytopathic effect Efficacy expression extrinsic pathway flow cytometry IBV IBV infection Immune cell immune cells Infection Infectious Bronchitis Virus inhibited inhibitory intrinsic Macrophage macrophage cell line mammalian cell mechanism morphological changes offer previous study primary cells Replication reported Research suggested viability virus replication was increased [DOI] 10.3390/v9080198 PMC 바로가기 [Article Type] Article
Secretion of Rhoptry and Dense Granule Effector Proteins by Nonreplicating Toxoplasma gondii Uracil Auxotrophs Controls the Development of Antitumor Immunity비복제 Toxoplasma gondii Uracil Auxotrophs에 의한 Rhoptry 및 Dense Granule Effector 단백질의 분비는 항종양 면역의 발달을 제어합니다Research Article Published on 2016-07-222022-09-06 Journal: PLoS Genetics [Category] Communicable Disease, [키워드] abrogated activate activated acute infection Auxotroph auxotrophic mutants axis CD4 CD8 Cell Nucleus Cell signaling cellular signaling pathway cellular signaling pathways conjunction Control deleted Deletion dendritic cells Dense development effective effective therapy effector effector molecule effector molecules effector protein effector proteins function Granule GTPase GTPases Host host cell host cell nucleus host cell response Host immune response host immune responses host innate immunity host response host responses identify IFN-γ immune responses Immune suppression Immunity independent Infection interferon Invasion latent infection mammalian cell mechanism membrane mice mutant not affect nucleus ovarian cancer parasite pathway Pathways Protein required responses rhoptry Safe secreted secretion shown Signaling solid tumor solid tumors survival T cell T cells target therapeutic These data Toxoplasma gondii transcriptional Trigger Tumor tumor microenvironment tumors type I uracil vaccination vaccine strain virulence [DOI] 10.1371/journal.pgen.1006189 PMC 바로가기 [Article Type] Research Article
S1 domain of the porcine epidemic diarrhea virus spike protein as a vaccine antigenResearch Published on 2016-04-012022-10-28 Journal: Virology Journal [Category] Coronavirus, MERS, [키워드] affinity age analysed binding Cell cellular receptor clinical parameter colostrum Complete contagious diarrhea disease effective ELISA epidemic diarrhea Epitopes eukaryotic exhibited expressed expression highest identify IgA IgG immune response immunization immunized improvement infecting Infection intramuscularly Lactogenic immunity less mammalian mammalian cell morbidity and mortality Mortality N-terminal neutralizing antibody Newborn no significant difference PEDV pregnant produced PROTECT Protein Recombinant S1 protein responsible Result S1 S1 domain S1 protein S1 subunit SDS-PAGE sera serum significantly lower Spike protein subunit Subunit vaccine tested titre transferred vaccination Vaccine vaccine antigen virus Virus neutralization virus neutralization assays virus spike protein yeast [DOI] 10.1186/s12985-016-0512-8 PMC 바로가기 [Article Type] Research
Infectious Bronchitis Virus Generates Spherules from Zippered Endoplasmic Reticulum MembranesResearch Article Published on 2013-10-222022-10-29 Journal: mBio [Category] Coronavirus, MERS, [키워드] acute respiratory syndrome alphaviruses Betacoronavirus candidate Cell cell line cell types cellular cellular membranes characterized cisternae CM complex coronavirus Coronaviruses Culture cultures cytoplasm electron Endoplasmic reticulum epithelial cell Ex vivo generate IBV IBV infection IBV-infected cell in viral induce infected cells Infection Infectious Bronchitis Virus interior invagination lack mammalian cell mean membrane mouse hepatitis virus organ outer membrane pathogen platform positive-sense positive-sense single-stranded RNA related replication complexes Reticulum RNA RNA synthesis RNA virus RNA viruses SARS-CoV sealed spherule spherules Structure thought tissue tomography tracheal Vesicle viral replication Viral RNA virus [DOI] 10.1128/mBio.00801-13 PMC 바로가기 [Article Type] Research Article
Structure and Inhibition of the SARS Coronavirus Envelope Protein Ion ChannelResearch Article Published on 2009-07-102022-10-29 Journal: PLoS Pathogens [Category] Coronavirus, [키워드] 2003 acute respiratory syndrome affecting alteration Anti-viral drugs antiviral strategy Author binding site C-terminal Cell channel characterized common cold coronavirus coronavirus protein described determined distribution drug design E protein embryonic kidney envelope envelope protein event expressed expressing feature form found full length HEK-293 Human humans in vitro inactivation infect inhibited inhibition Ion lethal disease mammalian cell mammals membrane Mild morphology N-terminal NMR pandemic Pathogenesis pharmacological target platform produced Protein Proteins provide reduced reported responsible SARS-CoV SARS-CoV E shown significantly Structure supported transmembrane domain Validity variety viral envelope viral pathogen viral replication virion virus viruses [DOI] 10.1371/journal.ppat.1000511 PMC 바로가기 [Article Type] Research Article