Detection of respiratory pathogens by application of multiplex PCR panel during early period of COVID-19 pandemic in a tertiary hospital in Central TaiwanResearch article Published on 2021-10-012022-10-05 Journal: Journal of microbiology, immunology, and infection [Category] 치료기술, [키워드] Adults age analyzed Atypical pathogen children China clinical presentation Cohort collected conducted COVID-19 pandemic detect detection detection rate Epidemiology group hospital Human IMPROVE incidence Infection less multiplex Multiplex PCR Mycoplasma pneumoniae nucleic acid pathogen Pathogens polymerase chain positive rate predominant real time respiratory respiratory infections respiratory pathogen Respiratory pathogens respiratory sample respiratory tract infection Result retrospective cohort study Taiwan tested university virus [DOI] 10.1016/j.jmii.2021.09.011 [Article Type] Research article
Aerosol SARS-CoV-2 in hospitals and long-term care homes during the COVID-19 pandemicCovid-19 Pandemic 동안 병원 및 장기 치료 가정에서 에어로졸 SARS-COV-2Research Article Published on 2021-09-302022-09-10 Journal: PLoS ONE [Category] MERS, SARS, 치료기술, 치료제, [키워드] aerosol Care close contact collected Concentration COVID-19 pandemic examined experiment gelatin hospital hospital ward ICU in viral indicated information Long-term care micrometer Multiplex PCR N protein no significant difference not recovered outbreak Outbreaks Particle positive positive patient positive patients quantified Result risk RNA RNA sample RNA samples Sample SARS-CoV-2 SARS-CoV-2 virus stomatitis TCID the patient Transmission Ultrasonic vesicular stomatitis vesicular stomatitis virus viability Viral viral genome Viral RNA virus VSV with COVID-19 [DOI] 10.1371/journal.pone.0258151 PMC 바로가기 [Article Type] Research Article
A Novel Real-Time RT-PCR-Based Methodology for the Preliminary Typing of SARS-CoV-2 Variants, Employing Non-Extendable LNA Oligonucleotides and Three Signature Mutations at the Spike Protein Receptor-Binding DomainArticle Published on 2021-09-272022-10-29 Journal: International Review of Cell and Molecular Biology [Category] COVID-19, [키워드] ACE2 amino-acid amplification Analysis approach Beta blocker Delta delta variant domain Efficiency examined expand Gamma Human identify Immune escape linear LNA oligonucleotide blockers log Multiplex PCR mutated Mutation negative sample non-specific novel nucleic acid oligonucleotide other variant probe Public health interventions Rapid RBD RBM real-time RT-PCR Receptor-binding domain receptor-binding motif reduce residue resulting SARS-CoV-2 SARS-CoV-2 variant signature Spike protein Substitution TaqMan targets the SARS-CoV-2 the Spike the spike protein Transmissibility typing validation variant variant of concern variants variants of concern [DOI] 10.3390/life11101015 PMC 바로가기 [Article Type] Article
In-house reverse transcriptase polymerase chain reaction for detection of SARS-CoV-2 with increased sensitivity증가 된 감도와 SARS-COV-2의 검출을위한 사내 역전사 효소 중합 효소 연쇄 반응Article Published on 2021-09-092022-09-01 Journal: Scientific Reports [Category] SARS, 변종, 진단, 치료기술, 치료제, [키워드] added advent amplification amplify amplifying applicability approach concentrations Concordance COVID-19 disease COVID-19 infection COVID-19 test demonstrated Dilution Epidemiology help management multiplex Multiplex PCR Multiplexing offer pandemic PCR condition polymerase chain positive sample primer Primers RdRP real time Real-time PCR relief resource RT-PCR SARS-CoV-2 SARS-CoV-2 genes sensitivity sets of primers shown significantly SYBR Green the SARS-CoV-2 transcriptase variable [DOI] 10.1038/s41598-021-97502-1 PMC 바로가기 [Article Type] Article
Co-infection of ST2 IP carbapenem-resistant Acinetobacter baumannii with SARS-CoV-2 in the patients admitted to a Tehran tertiary referral hospitalResearch Published on 2021-09-082022-10-28 Journal: BMC Infectious Diseases [Category] COVID-19, [키워드] oxa23 oxa24 oxa58 Acinetobacter age antibiotic Antibiotic resistance carbapenem Carbapenem-resistant Acinetobacter baumannii (CRAB) carried caused clinical sample clone Co-infection concerning COVID-19 COVID-19 case COVID-19 pandemic COVID-19 patient COVID-19 patients demonstrated dissemination Genetic Gram-negative bacteria HAI high mortality hospital ICU identify Infection infection control isolate isolates Multiplex PCR outbreak outcome PCR performed recognize resistance gene Result SARS-CoV-2 screened sequence Sequence type ST2 susceptibility Tehran TEM tertiary referral hospital the mean the patient underlying disease was determined was recorded [DOI] 10.1186/s12879-021-06642-2 PMC 바로가기 [Article Type] Research
SARS-CoV-2 N gene dropout and N gene Ct value shift as indicator for the presence of B.1.1.7 lineage in a commercial multiplex PCR assay상용 멀티플렉스 PCR 분석에서 B.1.1.7 계통의 존재에 대한 지표로서의 SARS-CoV-2 N 유전자 탈락 및 N 유전자 Ct 값 이동Article Published on 2021-09-012022-09-11 Journal: Clinical microbiology and infection : the official [Category] SARS, 변종, 진단, 치료기술, 치료법, [키워드] analysed Analysis approach B.1.1.7 B.1.1.7 lineage can be used characteristic cloned coding sequence Confirmed Ct value Ct values detection Diagnostic quantitative RT-PCR diagnostics evaluated experiments Genes Genetic identify Lineage Multiplex PCR Mutation N gene N genes Patient PCR PCR assay Plasmid plasmids polymorphism positive sample qRT-PCR Quantitative quantitative real-time PCR RdRP RdRp gene receiver operating characteristic SARS-CoV-2 SARS-CoV-2 B.1.1.7 SARS-CoV-2 N gene SARS-CoV-2 S shown significantly higher Surveillance variants variants of concern vector VoC VOC. VOCs whole-genome sequencing [DOI] 10.1016/j.cmi.2021.05.025 PMC 바로가기 [Article Type] Article
Combined use of a broad-panel respiratory multiplex PCR and procalcitonin to reduce duration of antibiotics exposure in patients with severe community-acquired pneumonia (MULTI-CAP): a multicentre, parallel-group, open-label, individual randomised trial conducted in French intensive care units중증 지역사회획득폐렴(MULTI-CAP) 환자의 항생제 노출 기간을 줄이기 위해 광범위 호흡 다중 PCR과 프로칼시토닌을 함께 사용: 프랑스어 집중적으로 수행된 다기관, 병렬 그룹, 공개 라벨, 개별 무작위 시험 케어 유닛Randomized Controlled Trial Published on 2021-08-182022-09-12 Journal: BMJ Open [Category] MERS, 임상, 진단, [키워드] 1:1 adult intensive & adult thoracic medicine Algorithm antibiotic Antibiotics approach approved Bacterial Biomarker clinical clinical criteria clinician Combined combined use Committee community-acquired pneumonia conducted control arm Critical Critical care days alive Declaration of Helsinki Diagnosis diagnostic Diagnostic microbiology drug Ethics excluded French Hypothesis Intensive intensive care intensive care unit intervention arm molecular molecular diagnostics molecular test multicentre Multiplex PCR Open-label parallel-group parenchymal Patient peer-reviewed performed Person Pneumonia primary endpoint procalcitonin protection public health randomisation randomised Randomised trial reduce reducing Registered respiratory infections Safety selection pressure Spread submitted the patient Thoracic Treatment Trial usual care written Informed Consent [DOI] 10.1136/bmjopen-2020-048187 PMC 바로가기 [Article Type] Randomized Controlled Trial
Catching SARS-CoV-2 by Sequence Hybridization: a Comparative AnalysisResearch Article Published on 2021-08-032022-10-29 Journal: mSystems [Category] COVID-19, [키워드] acute respiratory syndrome adaptive mutations approach approaches Bioscience circulating Community Comparative computation condition controlling coronavirus ddPCR determine digital PCR Efficiency efficient enrichment evaluate Evolution FIVE flow cell full-length genome Genome geographical distribution Illumina improvement Laboratory MONITOR Multiplex PCR Mutation mutations Next-generation sequencing NGS pandemic question resources SARS-CoV-2 SARS-CoV-2 sequencing SARS-CoV-2 strain sensitivity sequence sequence capture Sequencing the patient the SARS-CoV-2 virus Twist viral spread virus [DOI] 10.1128/mSystems.00392-21 PMC 바로가기 [Article Type] Research Article
Evaluation of a fully automated high-throughput SARS-CoV-2 multiplex qPCR assay with built-in screening functionality for del-HV69/70- and N501Y variants such as B.1.1.7B.1.1.7과 같은 del-HV69/70- 및 N501Y 변이체에 대한 스크리닝 기능이 내장된 완전 자동화된 고처리량 SARS-CoV-2 다중 qPCR 분석 평가Article Published on 2021-08-012022-09-11 Journal: Journal of Clinical Virology [Category] SARS, 변종, 치료기술, [키워드] analytical sensitivity automated B.1.1.7 B.1.1.7 lineage B.1.351 caused clinical sample clinical samples Del-HV69/70 diagnostic England gold gold standard hand High-throughput Lineage lineages multiplex Multiplex assay Multiplex PCR N501Y N501Y mutation New PCR assay platform qPCR qPCR assay RT-qPCR RT-qPCR assay RT-qPCR assays SARS-CoV-2 SARS-CoV-2 RNA SARS-CoV-2 variant sensitivity Sequencing serial dilution SNP South Africa Spread tested Transmissibility variant variants wild-type SARS-CoV-2 [DOI] 10.1016/j.jcv.2021.104894 PMC 바로가기 [Article Type] Article
Influenza co-infection associated with severity and mortality in COVID-19 patientsCOVID-19 환자의 중증도 및 사망률과 관련된 인플루엔자 공동 감염Research Published on 2021-06-142022-09-10 Journal: Virology Journal [Category] MERS, SARS, 신종인플루엔자, 치료기술, [키워드] Adenovirus bacterial co-infection Chlamydia pneumoniae clinical implication clinical outcomes co-infecting Co-infection Coverage COVID-19 COVID-19 pandemic COVID-19 patient COVID-19 patients Critically ill Critically ill patient critically ill patients death died disease severity Evidence evidence of flu vaccination H1N1 highlight Hospitalization ICU ICU admission implication Influenza influenza A Influenza A H1N1 Influenza virus investigated mitigate Mortality Multiplex PCR Nasopharyngeal samples non-ICU outcome pathogen Pathogens Prevalence reduce respiratory respiratory pathogen Respiratory pathogens Result risk SARS-CoV-2 screened severity Transmission treatment approach Viral virus viruses [DOI] 10.1186/s12985-021-01594-0 PMC 바로가기 [Article Type] Research