Ritonavir may inhibit exoribonuclease activity of nsp14 from the SARS-CoV-2 virus and potentiate the activity of chain terminating drugsRitonavir는 SARS-CoV-2 바이러스로부터 nsp14의 엑소리보뉴클레아제 활성을 억제하고 연쇄 종결 약물의 활성을 강화할 수 있습니다Article Published on 2021-01-312022-09-11 Journal: International journal of biological macromolecules [Category] SARS, 신약개발, 치료제, [키워드] active site association attenuate binding binding site calculated causative agent complex Computational study Coronaviridae family COVID19 drug duplication effective inhibitor Exoribonuclease Favipiravir FDA approved drug Genome homology model identify in silico inhibit inhibitor inhibitory effect molecules nsp10 nsp14 nucleotide overlap pandemic premature termination Prevent Protein Remdesivir responsible ribavirin Ritonavir RNA SARS-CoV-2 suggested the Coronaviridae the SARS-CoV-2 virus therapeutic Viral viral genome replication virus was used [DOI] 10.1016/j.ijbiomac.2020.12.038 PMC 바로가기 [Article Type] Article
Molecular docking studies of some selected gallic acid derivatives against five non-structural proteins of novel coronavirusResearch Published on 2021-01-252022-10-30 Journal: Journal of Genetic Engineering & Biotechnology [Category] COVID-19, [키워드] 3D structure binding energy Cardiomyopathy Chloroquine clinical trial Compound COVID-19 COVID-19 infected patient crystal structure database derivative detrimental Dexamethasone disease docked drug Druglikeness drugs Favipiravir FIVE Gallic acid derivatives health emergency Hydroxychloroquine in silico in vitro study in vivo inhibitor Ivermectin liver lower binding energy molecular molecular docking Molecular docking study molecular interactions MPro New coronavirus non-structural protein non-structural proteins Novel coronavirus Nsp12 nsp13 nsp14 Nsp15 Nsp3 pandemic PDB phosphate Plasma Protein Binding Protein Proteins PubChem Remdesivir reported Result retrieved ribavirin SARS-CoV-2 selected Side effect small molecule small molecule inhibitor Support therapeutic compound Vaccine virus World Health Organization [DOI] 10.1186/s43141-021-00120-7 PMC 바로가기 [Article Type] Research
Molecular Docking Reveals Ivermectin and Remdesivir as Potential Repurposed Drugs Against SARS-CoV-2Microbiology Published on 2021-01-252022-10-31 Journal: Frontiers in Microbiology [Category] COVID-19, [키워드] ACE-2 ACE-2 receptor addition Against Antiviral antiviral agent bind cause caused Cell Chloroquine chloroquine/hydroxychloroquine clinical trial conducted Consequences coronavirus coronavirus disease COVID-19 docking drug drugs evaluated Favipiravir global pandemic Hydroxychloroquine inhibitors of SARS-CoV-2 Ivermectin M protein mechanism membrane fusion Membrane proteins molecular dynamics nsp14 Potential Proteases RdRP Remdesivir remdesivir (GS-5734) Replication reported Repurposed drugs Respiratory disease RNA RNA polymerase SARS-CoV-2 screened severity Stage surface receptor target target proteins the binding affinity therapeutic agents TMPRSS2 treatment protocols Trigger viral replication viral spike protein was performed Wuhan [DOI] 10.3389/fmicb.2020.592908 PMC 바로가기 [Article Type] Microbiology
The coronavirus proofreading exoribonuclease mediates extensive viral recombination코로나바이러스 교정 엑소리보뉴클레아제는 광범위한 바이러스 재조합을 매개합니다Research Article Published on 2021-01-192022-08-13 Journal: PLoS Pathogens [Category] Biochemistry, MERS, 유전자 메커니즘, 진단, 치료제, [키워드] attenuation conserved coronavirus coronavirus exoribonuclease coronavirus proofreading exoribonuclease Coronavirus replication CoV CoV polymerase CoV recombination CoV replication CoVs Critical Culture defective viral genome determinant determinants Diversity DVGs ExoN Exoribonuclease Frequency generate highlight immune evasion implicated inactivation Infection inhibitor inhibitors loci maintain mechanism MERS MERS-CoV MHV MHV nsp14-ExoN molecular mechanism molecular mechanisms murine hepatitis virus novel coronaviruses nsp14 nsp14-ExoN nucleoside Nucleoside analogues outbreak pandemic Population Prevent produced Recombination recombination frequency recombination junctions Replication required responsible RNA RNA proofreading exoribonuclease RNA recombination RNA synthesis SARS-CoV-2 sequence sequences similarity sub-genomic mRNA sub-genomic mRNAs These data Viral viral recombination virulence while zoonotic CoVs [DOI] 10.1371/journal.ppat.1009226 PMC 바로가기 [Article Type] Research Article
Global analysis of more than 50,000 SARS-CoV-2 genomes reveals epistasis between eight viral genes530 Published on 2020-12-082022-10-29 Journal: Proceedings of the National Academy of Sciences of [Category] COVID-19, MERS, SARS, [키워드] acute respiratory syndrome Analysis assumption caused Complete coronavirus COVID-19 pandemic Cutoff direct coupling analysis Effect eight epistasis Genome genome sequence GISAID in viral initiative Interaction interactions investigated loci locus microbial Mutation natural selection Nonsynonymous mutations Nsp12 nsp13 nsp14 NSP2 nsp4 ORF3a ORF8 Pathogenesis Pathogens powerful tool public health emergency Recombination Repository reveal SARS-CoV-2 SARS-CoV-2 genome sequence Significance starting point synonymous Vaccine development viral gene viral pathogens Whole-genome sequence [DOI] 10.1073/pnas.2012331117 PMC 바로가기 [Article Type] 530
1 H, 13 C, and 15 N backbone chemical shift assignments of coronavirus-2 non-structural protein Nsp10Article Published on 2020-11-072022-10-27 Journal: Biomolecular Nmr Assignments [Category] COVID-19, [키워드] backbone Coronavirus-2 COVID19-NMR drugs element ENCODE function genomic in viral innate immune system Interaction International involved Ligand methyltransferase molecular component NMR non-structural protein nsp14 Nsps PROTECT Protein Proteins Replication replication-transcription complex RNA RNA genome RNA-dependent RNA polymerase RTC SARS-CoV-2 SARS-CoV-2 genome SARS-CoV-2 RNA Solution NMR-spectroscopy structural proteins Structure subgenomic RNAs transcriptional variety Via viral envelope viral life cycle Viral RNA virus [DOI] 10.1007/s12104-020-09984-1 PMC 바로가기 [Article Type] Article
1 H, 13 C, and 15 N backbone chemical shift assignments of coronavirus-2 non-structural protein Nsp10Article Published on 2020-11-072022-10-27 Journal: Biomolecular Nmr Assignments [Category] Coronavirus, COVID-19, SARS, [키워드] backbone Coronavirus-2 COVID19-NMR drugs element ENCODE function genomic in viral innate immune system Interaction International involved Ligand methyltransferase molecular component NMR non-structural protein nsp14 Nsps PROTECT Protein Proteins Replication replication-transcription complex RNA RNA genome RNA-dependent RNA polymerase RTC SARS-CoV-2 SARS-CoV-2 genome SARS-CoV-2 RNA Solution NMR-spectroscopy structural proteins Structure subgenomic RNAs transcriptional variety Via viral envelope viral life cycle Viral RNA virus [DOI] 10.1007/s12104-020-09984-1 PMC 바로가기 [Article Type] Article
Mutations of SARS-CoV-2 nsp14 exhibit strong association with increased genome-wide mutation loadBioinformatics Published on 2020-10-122022-10-28 Journal: PeerJ [Category] COVID-19, [키워드] 3′-5′ exonuclease affecting analyzed association Betacoronavirus combating complex COVID-19 exonuclease Genome genomic human host infection rate isolates mutability Mutation mutation rate Nsp12 nsp14 observation pandemic polymerase predictor Protein Proteins RdRP Replication Research responsible RNA genome RNA synthesis RNA-dependent RNA polymerase SARS-CoV-2 SARS-CoV-2 isolate selective shown significantly Spread Strains survival the disease viral genome viral isolate virus wildtype zoonotic origin [DOI] 10.7717/peerj.10181 PMC 바로가기 [Article Type] Bioinformatics
The Enzymatic Activity of the nsp14 Exoribonuclease Is Critical for Replication of MERS-CoV and SARS-CoV-2Genome Replication and Regulation of Viral Gene Expression Published on 2020-09-162022-10-30 Journal: Journal of Virology [Category] COVID-19, SARS, [키워드] activities activity acute respiratory syndrome affecting Alpha Amino acid antiviral drug Betacoronavirus betacoronaviruses boost can be used complex coronavirus CoV CoVs Critical decrease displaying domains enzymatic activity enzyme evaluated example Exoribonuclease Frequency Genetic genetics genome replication guanine-N7-methyltransferase In vitro assay MERS-CoV MHV Middle East MONITOR mouse hepatitis virus mRNA mutant mutants Mutation nidovirus nonstructural protein nsp14 Nsps observation occurred phenotype Phenotypes progeny promote Proofreading purified replicase replicate Replication replication fidelity replicative reported respiratory syndrome coronavirus reverse genetics RNA genome RNA synthesis SARS-CoV SARS-CoV-2 sequence identity single mutant subunit Support thought viral RNA synthesis viruses while [DOI] 10.1128/JVI.01246-20 PMC 바로가기 [Article Type] Genome Replication and Regulation of Viral Gene Expression
Coronavirus RNA Proofreading: Molecular Basis and Therapeutic TargetingReview Published on 2020-08-042023-06-14 Journal: Molecular Cell [Category] MERS, SARS, [키워드] anti-coronavirus drugs Antisense oligonucleotide ASO coronavirus CoV ExoN exonuclease NA non-structural protein 14 nsp14 nucleoside analog [DOI] 10.1016/j.molcel.2020.07.027 PMC 바로가기 [Article Type] Review