An evaluation of a FluoroSpot assay as a diagnostic tool to determine SARS-CoV-2 specific T cell responsesSARS-COV-2 특이 적 T 세포 반응을 결정하기위한 진단 도구로서 플루오 스팟 분석의 평가Research Article Published on 2021-09-302022-09-10 Journal: PLoS ONE [Category] SARS, 진단, 치료법, [키워드] addition Affect aligning Amino acid appear assays blood sample Blood samples calculated cellular Cellular immune response cellular immune responses cellular response Chain Reaction Cohort COVID-19 patients cross-reactive determine diagnostic diagnostic value dual endemic HCoVs evaluated HCoVs HCW healthcare worker Healthcare workers hospitalized COVID-19 patient house human coronavirus human coronaviruses IFN-γ IgG immune response immune responses individuals less nucleocapsid Numerous peptide peptide pool peptide pools polymerase chain polymerase chain reaction post infection reference standard Release repeated Research SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 spike sensitivity Seroconversion serological serological assays serology seronegative Specificities specificity T cell T cell response [DOI] 10.1371/journal.pone.0258041 PMC 바로가기 [Article Type] Research Article
Serological Assays for Assessing Postvaccination SARS-CoV-2 Antibody Response백신 접종 후 SARS-CoV-2 항체 반응을 평가하기 위한 혈청학적 분석Research Article Published on 2021-09-292022-09-10 Journal: Microbiology Spectrum [Category] MERS, SARS, 진단, 치료기술, 치료법, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 antibody Assessing balance best characteristic CLIA confidence coronavirus Cost effectiveness country COVID-19 COVID-19 pandemic Cutoff cutoff value DiaSorin Elecsy Elecsys ELISA enzyme evaluated False negative false negatives gold gold standard highest highlight IgG immune response Immune status immunosorbent knowledge limitation mass neutralization neutralization test optimization pandemic parameter plaque reduction neutralization Population positive postvaccination Predictive predictive values PRNT receiver receiver operating characteristic required respiratory response ROC Roche S1/S2 SARS-CoV-2 SARS-CoV-2 antibodies SARS-CoV-2 IgG sensitivity Serological assay serological assays severe acute respiratory syndrome Coronavirus significantly increased specificity surrogate virus neutralization test Surveillance understanding vaccinate vaccination virus neutralization test [DOI] 10.1128/Spectrum.00733-21 PMC 바로가기 [Article Type] Research Article
EpiSurf: metadata-driven search server for analyzing amino acid changes within epitopes of SARS-CoV-2 and other viral speciesOriginal Article Published on 2021-09-292022-11-01 Journal: Database: The Journal of Biological Databases and [Category] COVID-19, SARS, [키워드] alterations amino acid change amino acid changes change COG-UK Complete COVID-19 curated database distribution drug endpoints epitope Epitopes GenBank Genomics GISAID sequences Immune Epitope Database Influenza Lineage Metadata mutated offer overlapping Population Region SARS-CoV-2 searched selected sequence serological assays Support Vaccines Viral protein Web web application [DOI] 10.1093/database/baab059 PMC 바로가기 [Article Type] Original Article
Impact of Specific N -Glycan Modifications on the Use of Plant-Produced SARS-CoV-2 Antigens in Serological AssaysPlant Science Published on 2021-09-272022-10-31 Journal: Frontiers in Plant Science [Category] COVID-19, [키워드] allergen allergic antibodies antibody Antigen average carbohydrate Cell characterized comparable contribute COVID-19 cross-reactive cross-reactive carbohydrate determinant detect determinant epitope event expressed expression False positive False positives glycosylated glycosylation human cell human sera humans immune response Impact individual Interaction Modification Neutralizing antibodies Nicotiana benthamiana pandemic plant populations posttranslational modification produced RBD reactivity Receptor binding domain reduced required SARS-CoV-2 sera serological assays serological test Specific Spike protein the SARS-CoV-2 Transient variant viral antigens viral infection virus was used while wild-type [DOI] 10.3389/fpls.2021.747500 PMC 바로가기 [Article Type] Plant Science
Immunological assessment of SARS-CoV-2 infection in pregnancy from diagnosis to delivery: A multicentre prospective study진단에서 전달까지 임신 중 SARS-COV-2 감염의 면역 학적 평가 : 다기관 전향 연구Observational Study Published on 2021-09-202022-09-10 Journal: PLoS ONE [Category] MERS, SARS, 임상, 진단, 치료기술, [키워드] 95% CI Abbott Abbott SARS-CoV-2 IgG All participants Antenatal anti-SARS-CoV-2 antibodies anti-SARS-CoV-2 antibody anti-SARS-CoV-2 IgG anti-SARS-CoV-2 IgG antibodies anti-SARS-CoV-2 IgG antibody antibody Antibody Response Antibody responses Architect Asymptomatic baseline Blood Cohort Combination conducted Cord blood demonstrated detectable Diagnosis Elecsys Europe evidence of fetal FIVE gestation hospital IgG IgG antibodies IgG SARS-CoV-2 antibodies IgG SARS-CoV-2 antibody IgM IgM antibodies immunoassay independent Informed Maternal maternity hospitals Migration multicentre Naso-pharyngeal swab naso-pharyngeal swabs Nasopharyngeal swab nasopharyngeal swabs Neonate observational study Participants passive immunity platform positive Pregnancy pregnant women Prevalence prospective observational study Prospective Study provided raising remained responses Result RNA Roche RT-PCR SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 RNA SARS-CoV2 serological assays serological assessment serological evidence serological response serological test seropositive serum serum sample Seven symptomatic the antibody response Transplacental umbilical Umbilical cord Viral Viral RNA was measured was obtained was performed women written consent [DOI] 10.1371/journal.pone.0253090 PMC 바로가기 [Article Type] Observational Study
Sera Neutralizing Activities Against Severe Acute Respiratory Syndrome Coronavirus 2 and Multiple Variants 6 Months After Hospitalization for Coronavirus Disease 2019중증 급성 호흡기 증후군 코로나바이러스 2 및 다중 변이체에 대한 혈청 중화 활성 코로나바이러스 질병 2019로 입원 후 6개월Article Published on 2021-09-152022-09-11 Journal: Clinical Infectious Diseases: An Official Publicat [Category] SARS, 변종, 진단, 치료법, [키워드] activity acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 addition Admission Against anti-S Anti-spike antibodies antibody B.1.1.7 B.1.351 B.1.351 variant characterized collected coronavirus coronavirus 2 coronavirus disease Coronavirus disease 2019 correlated correlation COVID-19 D614G effective Efficiency Evolution experiment Hospitalized humoral Humoral response IgG IgG level IgG levels Immunoglobulin Immunoglobulin G initial intensive care unit intensive care units invasive ventilation Level marker Month Multiple neutralization Neutralizing activity neutralizing capacity neutralizing effect occur P.1 variant P.1 variants Patient patients hospitalized performed Postinfection Prospective Study quantified Quantitative respiratory SARS-CoV-2 SARS-CoV-2 variant SARS-CoV-2 variants sera serological Serological assay serological assays severe acute respiratory syndrome Coronavirus severity significantly sustained tested the patient variant with COVID-19 [DOI] 10.1093/cid/ciab308 PMC 바로가기 [Article Type] Article
Serological assessment of SARS-CoV-2 infection during the first wave of the pandemic in Louisville Kentucky루이빌 켄터키에서 유행병의 첫 번째 물결 동안 SARS-COV-2 감염의 혈청 학적 평가Article Published on 2021-09-142022-09-01 Journal: Scientific Reports [Category] SARS, 진단, 치료법, [키워드] Adaptive immunity Analysis Antibody responses Antibody titer Biotechnology Characteristics defined Diagnosis Diseases ELISA enzyme enzyme-linked immunosorbent assay First wave healthcare worker Healthcare workers Humoral immunity IgA IgA antibodies IgA antibody IgG IgM response IgM responses immune response immunology individual Infection Infectious diseases lack Local microneutralization neutralization neutralization assays neutralize nucleocapsid pandemic performance plaque positive correlation Protective Protective antibody Proteins RBD Receptor binding domain SARS-CoV-2 SARS-CoV-2 antibody SARS-CoV-2 antigen SARS-CoV-2 antigens SARS-COV-2 infection SARS-CoV-2 pandemic Sensitivity and specificity serological assays seropositive spike the antibody response the SARS-CoV-2 vaccination was used [DOI] 10.1038/s41598-021-97423-z PMC 바로가기 [Article Type] Article
Development and Evaluation of Quantitative Immunoglobulin G Enzyme-Linked Immunosorbent Assay for the Diagnosis of Coronavirus Disease 2019 Using Truncated Recombinant Nucleocapsid Protein as Assay Antigen분석 항원으로 절단된 재조합 뉴클레오캡시드 단백질을 사용한 코로나바이러스 질병 2019 진단을 위한 정량적 면역글로불린 G 효소 연결 면역흡착 분석법의 개발 및 평가Article Published on 2021-09-132022-09-10 Journal: International Journal of Environmental Research an [Category] SARS, 진단, 치료법, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus antibodies Antigen applied can be used causative agent collected complementary conserved conserved region coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 COVID-19 diagnosis cross-reactive cross-reactive antibodies cross-reactive antibody deleted demonstrated development Diagnosis E. coli ELISA evaluate full-length gold IgG IgG ELISA IgG indirect ELISA Immunoglobulin Immunoglobulin G less N protein negative control neutralization test nucleocapsid protein plaque reduction neutralization Plaque reduction neutralization test prevalent PRNT Protein protein expression real-time RT-PCR recombinant respiratory RT-PCR SARS-CoV-2 SARS-CoV-2 antibodies SARS-CoV-2 antibody SARS-CoV-2 N SARS-CoV-2 N protein SARS-CoV-2 spike protein sensitivity sera Serological assay serological assays severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 specificity spike IgG Spike protein Surveillance [DOI] 10.3390/ijerph18189630 PMC 바로가기 [Article Type] Article
Health care worker seromonitoring reveals complex relationships between common coronavirus antibodies and COVID-19 symptom duration의료 종사자 혈청 모니터링은 일반적인 코로나 바이러스 항체와 COVID-19 증상 기간 사이의 복잡한 관계를 보여줍니다Research Article Published on 2021-08-232022-09-10 Journal: JCI Insight [Category] MERS, SARS, 유전자 메커니즘, 진단, [키워드] Adaptive immunity antibody Antibody titer Antibody titers Betacoronavirus CCV Cellular immune response Cohort collected complex coronavirus COVID-19 COVID-19 severity COVID-19 symptom cross-reactive antibodies cross-reactive antibody duration of symptom elicited Health care Health care worker health care workers Hypothesis identify immune responses immunology individual Infection infections limit mechanism reduce reduced reveal SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 infections Serological assay serological assays significantly symptom duration [DOI] 10.1172/jci.insight.150449 PMC 바로가기 [Article Type] Research Article
SARS-CoV-2 IgG response in symptomatic and asymptomatic COVID-19-infected healthcare workers코로나19에 감염된 유증상 및 무증상 의료 종사자의 SARS-CoV-2 IgG 반응Article Published on 2021-08-202022-09-11 Journal: Occupational medicine (Oxford, England) [Category] MERS, SARS, 진단, 치료기술, [키워드] accounted affected age analysed anti-SARS-CoV-2 IgG anti-SARS-CoV-2 IgG antibodies antibody Asymptomatic COVID-19 COVID-19 COVID-19 infection COVID-19 infections COVID-19 symptom COVID-19 symptoms detectable develop evaluate Factor HCW HCWs healthcare healthcare worker Healthcare workers humoral Humoral response identify IgG IgG antibodies independent Infection infection with SARS-CoV-2 Interpretation Logistic regression occupational medicine Older positive proportion recruited RT-PCR SARS-CoV-2 SARS-CoV-2 IgG SARS-CoV-2 IgG antibodies serological assays serology serology test Seropositivity seroprevalence survey serum significantly more symptomatic symptomatic and asymptomatic the SARS-CoV-2 was performed [DOI] 10.1093/occmed/kqab061 PMC 바로가기 [Article Type] Article