Hetero-bivalent nanobodies provide broad-spectrum protection against SARS-CoV-2 variants of concern including OmicronArticle Published on 2022-07-292022-10-05 Journal: Cell Research [Category] COVID19(2023년), SARS, 변종, 유전자 메커니즘, 진단, 치료제, [키워드] adaptive antibody benefit Beta binding binding affinity broad-spectrum antiviral caused complex Contact COVID-19 crystal structure Delta ENhance epitope fused fusing Gamma hamster Infection Lambda mice mouse-adapted SARS-CoV-2 Mutation nanobody neutralization activity neutralized omicron Prophylactic prophylactically provide provided Pseudotyped viruses RBD RBD residue resolved SARS-CoV-2 SARS-CoV-2 variant tested the RBD the receptor-binding domain the spike protein therapeutic Treatment variant variants variants of concern wild-type Wuhan [DOI] 10.1038/s41422-022-00700-3 [Article Type] Article
Urtica dioica Agglutinin: A plant protein candidate for inhibition of SARS-COV-2 receptor-binding domain for control of Covid19 InfectionUrtica dioica Agglutinin: Covid19 감염 통제를 위한 SARS-COV-2 수용체 결합 도메인 억제를 위한 식물성 단백질 후보Article Published on 2022-07-282022-09-11 Journal: PLoS ONE [Category] COVID19(2023년), MERS, SARS, 유전자 메커니즘, 진단, [키워드] ACE2 addition angiotensin Angiotensin-converting enzyme angiotensin-converting enzyme-2 Antiviral effect average bind binding binding affinity binding energy binding free energy binding site calculated Cell complex convalescent serum coronavirus docked docking docking result docking results domain Donor donors dynamic effective effective drug effective drugs ELISA ELISA assay enzyme exhibited free energy Gromacs host cell human cells IgG Infection inhibit inhibitory activity Interaction limitation MD simulation mechanism Medicine Medicines MTT Mutation peptide Prevent Previous studies Protein RBD RBD binding RBD complex RBD protein Receptor-binding domain Result S-protein Safe SARS-CoV SARS-CoV-2 Simulation spike Spike protein spike protein of SARS-CoV-2 stability the cell the RBD Toxicity Urtica Vaccine virus was performed was used [DOI] 10.1371/journal.pone.0268156 PMC 바로가기 [Article Type] Article
All-Atom Simulations of Human ACE2-Spike Protein RBD Complexes for SARS-CoV-2 and Some of its Variants: Nature of Interactions and Free Energy Diagrams for Dissociation of the Protein ComplexesSARS-CoV-2 및 일부 변이체에 대한 인간 ACE2-스파이크 단백질 RBD 복합체의 전체 원자 시뮬레이션: 단백질 복합체의 해리를 위한 상호작용 및 자유 에너지 다이어그램의 특성Article Published on 2022-07-282022-09-11 Journal: The journal of physical chemistry. B [Category] COVID19(2023년), SARS, 변종, [키워드] ACE2 Analysis atomic level average Comparative analysis Diagram dissociation electrostatic electrostatic interaction energy FIVE Free free energy Free energy calculation Free energy calculations Human human Ace2 protein hydrogen Hydrogen bond increase increase in Interaction investigated L452R molecular molecular dynamics mutant N501Y Nature nonspecific pathway positive Protein RBD RBD-ACE2 RBDs Receptor binding domain SARS-CoV-1 SARS-CoV-2 Simulation Spike protein the RBD the spike protein trajectory variant variants variants of SARS-CoV-2 wild type [DOI] 10.1021/acs.jpcb.2c00833 PMC 바로가기 [Article Type] Article
Short-Term Effect of SARS-CoV-2 Spike Protein Receptor-Binding Domain-Specific Antibody Induction on Neutrophil-Mediated Immune Response in Mice쥐의 호중구 매개 면역 반응에 대한 SARS-CoV-2 스파이크 단백질 수용체 결합 도메인 특이적 항체 유도의 단기 효과Article Published on 2022-07-262022-09-12 Journal: International Journal of Molecular Sciences [Category] COVID19(2023년), MERS, SARS, 진단, [키워드] Activation Affect airway alteration antibodies antibody antibody formation Antibody Response Blood bone marrow cell CD101 Control COVID-19 CXCR2 domain Effect elevate excessive inflammation immunized induction injection Innate immunity investigated mice mimicking mouse model NETs neutrophil Neutrophil extracellular trap neutrophil recruitment Neutrophils Particle phenotype phosphate buffer PROTECT RBD RBD-specific antibodies RBD-specific antibody Receptor-binding domain respiratory inflammation response SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 virus spike stimulate the RBD the spike protein triggering [DOI] 10.3390/ijms23158234 PMC 바로가기 [Article Type] Article
Characterization of SARS-CoV-2 Escape Mutants to a Pair of Neutralizing Antibodies Targeting the RBD and the NTDRBD 및 NTD를 표적으로 하는 한 쌍의 중화 항체에 대한 SARS-CoV-2 탈출 돌연변이의 특성화Article Published on 2022-07-252022-09-11 Journal: International Journal of Molecular Sciences [Category] COVID19(2023년), MERS, SARS, 변종, 진단, [키워드] active immunization Affect antibodies antibody binding Cell culture characterization Clinical use Cluster Combination Efficacy escape escape mutant escape mutants escape mutation gene segments Genetic human immunoglobulin identify immunization immunization strategies Immunoglobulin indicate lead mice monoclonal monoclonal antibodies monoclonal antibody more difficult Mutation mutations neutralization Neutralization assay Neutralizing Neutralizing antibodies neutralizing antibody neutralizing monoclonal antibodies neutralizing monoclonal antibody nine not affected NTD pair passage pseudotype RBD RBD mutations reduced SARS-CoV-2 SARS-CoV-2 escape mutations SARS-CoV-2 variant SARS-CoV-2 variants selected Spike protein targeting the RBD the spike protein variable region variant variants variants of concern VoC [DOI] 10.3390/ijms23158177 PMC 바로가기 [Article Type] Article
DNA aptamers inhibit SARS-CoV-2 spike-protein binding to hACE2 by an RBD- independent or dependent approachDNA 앱타머는 RBD-독립적 또는 의존적 접근에 의해 hACE2에 대한 SARS-CoV-2 스파이크-단백질 결합을 억제합니다Article Published on 2022-07-182022-09-11 Journal: Theranostics [Category] COVID19(2023년), MERS, SARS, 변종, 진단, [키워드] affinity anti-RBD anti-S2 Anti-spike approach aptamer best binding binding affinities binding affinity block can be used Candidates cells conserved coronavirus COVID-19 pandemic Delta develop diagnostic DNA DNA aptamer Efficacy ELISA fusion aptamer hACE2 IMPROVE independent Infection inhibit inhibit SARS-CoV-2 inhibitor Interaction Ligand ligands linker molecular New coronavirus omicron optimization outbreak Prevent RBD recognize reported S2-protein SARS-CoV-2 selection process sequence spike-protein the RBD therapeutic variant variants were measured WT spike [DOI] 10.7150/thno.74428 PMC 바로가기 [Article Type] Article
Peptide-Antibody Fusions Engineered by Phage Display Exhibit an Ultrapotent and Broad Neutralization of SARS-CoV-2 Variants파지 디스플레이가 조작한 펩티드-항체 융합은 SARS-CoV-2 변이체의 초강력 및 광범위한 중화를 나타냅니다Article Published on 2022-07-152022-09-11 Journal: ACS chemical biology [Category] COVID19(2023년), SARS, 변종, 진단, [키워드] affinities affinity approach approved B.1.1.529 Bivalent bivalent IgG breadth clinically Coverage COVID-19 demonstrated display domain dominant ENhance exacerbated fused fusion fusions generate implicated IMPROVE Infection infection assay low potency Mutation N-terminal domain N-terminus NAb NAbs naïve neutralization neutralization potency neutralize neutralizing antibody NTD omicron peptide peptides phage RBD RBD mutation RBD mutations S-protein SARS-CoV-2 SARS-CoV-2 variant SARS-CoV-2 variants Spike protein spread of COVID-19 tetravalent the RBD the spike protein therapeutic Therapeutics trimer variant variants variants of concern vulnerability [DOI] 10.1021/acschembio.2c00411 PMC 바로가기 [Article Type] Article
The Cellular Characterization of SARS-CoV-2 Spike Protein in Virus-Infected Cells Using the Receptor Binding Domain Binding Specific Human Monoclonal Antibodies수용체 결합 도메인 결합 특이적 인간 단일클론 항체를 사용하여 바이러스에 감염된 세포에서 SARS-CoV-2 스파이크 단백질의 세포 특성화Article Published on 2022-07-132022-09-11 Journal: Journal of Virology [Category] COVID19(2023년), MERS, SARS, 변종, 진단, [키워드] adopted antibody appearance appears attachment B cell B cells biological properties biological property can be used Cell cell-to-cell transmission characterization conformation conformational change convalescent COVID-19 patient convalescent COVID-19 patients convalescent patient convalescent patient serum Convalescent patients cytoplasmic demonstrated early stage early stages electron microscopy epitope Epitopes Evidence exhibited FIVE focus furin future therapeutic Golgi complex host cell Human human monoclonal antibodies Human monoclonal antibody immunofluorescence microscopy immunological indicated infected cells infected with SARS-CoV-2 Infection Interaction isolate isolates Microscopy mitigate MONITOR monoclonal new virus occur offer Protein provided RBD reagent Receptor binding domain receptor binding domain (RBD) recognize required S protein S protein cleavage S protein RBD SARS- CoV-2 SARS-CoV-2 SARS-CoV-2 clinical isolates SARS-CoV-2 spike SARS-CoV-2-infected cell SARS-CoV-2-infected cells Singapore Specific spike staining the RBD the S protein therapeutic These data Transmission Vaccine variant virus infection virus particles virus-infected cell virus-infected cells was used were used while [DOI] 10.1128/jvi.00455-22 PMC 바로가기 [Article Type] Article
Pre-Omicron Vaccine Breakthrough Infection Induces Superior Cross-Neutralization against SARS-CoV-2 Omicron BA.1 Compared to Infection AlonePre-Omicron 백신의 획기적인 감염은 단독 감염에 비해 SARS-CoV-2 Omicron BA.1에 대해 우수한 교차 중화를 유도합니다Article Published on 2022-07-122022-09-11 Journal: International Journal of Molecular Sciences [Category] COVID19(2023년), MERS, SARS, 변종, 진단, 치료법, [키워드] antibody antibody levels Antibody Response Antibody responses B.1 boost Breakthrough infection comparable convalescent convalescent sera convalescent serum correlated COVID-19 patient cross-neutralization cross-neutralizing antibodies cross-neutralizing antibody Delta disease elicited evade form Gamma had no higher affinity highlight Immunity individuals induce infecting Infection Live virus Mild moderate disease N-terminal domain neutralization neutralize Neutralizing Neutralizing activity Neutralizing antibodies neutralizing antibody neutralizing capacity NTD nucleocapsid omicron Omicron BA.1 Patient patients with moderate Protein Proteins raise RBD Receptor-binding domain remained retained SARS-CoV-2 SARS-CoV-2 variants sera spike Spike protein Spike proteins strain Strains Superior the disease the RBD the receptor-binding domain the Spike the spike protein Transmissibility vaccination Vaccine Variability variant variants variants of concern variants of concern (VOCs) virus-induced antibodies VoC VOCs [DOI] 10.3390/ijms23147675 PMC 바로가기 [Article Type] Article
Impact of the temperature on the interactions between common variants of the SARS-CoV-2 receptor binding domain and the human ACE2SARS-CoV-2 수용체 결합 도메인의 일반적인 변이체와 인간 ACE2 간의 상호작용에 대한 온도의 영향Article Published on 2022-07-072022-09-11 Journal: Scientific Reports [Category] COVID19(2023년), SARS, 변종, [키워드] 1:1 ACE2 ACE2 binding acquisition Alpha angiotensin approach B.1.1.529 B.1.1.7 B.1.351 B.1.617.1 B.1.617.2 Beta binding Delta dissimilarity experiment high accuracy human ACE2 human Angiotensin-converting enzyme Human angiotensin-converting enzyme 2 Impact Interaction kinetic L452R mutant Mutation offer omicron parameter parameters powerful tool Protein RBD RBD mutation RBD variants Receptor binding domain reproducibility Spike protein Spike protein receptor binding domain surface plasmon surface plasmon resonance temperature the RBD the SARS-CoV-2 the Spike variant wild type Wuhan [DOI] 10.1038/s41598-022-15215-5 PMC 바로가기 [Article Type] Article