Detectable Duration of Viable SARS-CoV-2, Total and Subgenomic SARS-CoV-2 RNA in Noncritically Ill COVID-19 Patients: a Prospective Cohort Study중증이 아닌 COVID-19 환자에서 생존 가능한 SARS-CoV-2, 전체 및 서브게놈 SARS-CoV-2 RNA의 감지 가능한 기간: 전향적 코호트 연구Article Published on 2022-06-292022-09-11 Journal: Microbiology Spectrum [Category] COVID19(2023년), MERS, SARS, 바이오마커, 치료기술, [키워드] 95% confidence interval acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Analysis clearance collected coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 Culture Determination determine diagnosed Duration Evidence Factor feasibility gold gold standard hazard ratio Ill Immunocompromised Immunocompromised patients infection control Infectivity interquartile range investigated Isolation Kinetics marker median time N gene Nasopharyngeal swab nasopharyngeal swabs negativity Patient Prospective prospective cohort receipt Reinfection respiratory reverse transcription PCR RNA RNA RT-PCR RT-PCR SARS-CoV-2 SARS-CoV-2 RNA SARS-CoV-2 sgRNA SARS-CoV-2 sgRNAs severe acute respiratory syndrome Coronavirus sgRNA sgRNAs steroid subgenomic RNA subgenomic RNAs surrogate markers time Total total RNA transcript Viable Viral viral culture viral infectivity Viral RNA viral shedding [DOI] 10.1128/spectrum.00503-22 PMC 바로가기 [Article Type] Article
Receptor-Binding-Motif-Targeted Sanger Sequencing: a Quick and Cost-Effective Strategy for Molecular Surveillance of SARS-CoV-2 Variants수용체 결합 모티브 표적 생어 시퀀싱: SARS-CoV-2 변이체의 분자 감시를 위한 빠르고 비용 효율적인 전략Article Published on 2022-06-292022-09-11 Journal: Microbiology Spectrum [Category] Coronavirus, COVID19(2023년), SARS, 변종, 치료법, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 adopted aligned Alpha Alpha variant B.1.1.529 B.1.1.7 B.1.617.2 causative agent changes in CLC Clinical use coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 COVID-19 pandemic Critical death Delta delta variant develop disease spread Genome gold gold standard Infection involved Lineage lineages modifications molecular mutate Mutation mutations nasal nasal swab nasal swabs nucleocapsid omicron Omicron variant Omicron variants pandemic prevalent Prevent public health receptor-binding motif Receptor-binding motif (RBM) resource Saliva Sanger sequencing SARS-CoV-2 SARS-CoV-2 variant SARS-CoV-2 variants sequence sequences Sequencing severe acute respiratory syndrome Coronavirus Spike protein Spread Strategy Surveillance swab samples the disease variant variant surveillance. variants WGS whole-genome sequencing [DOI] 10.1128/spectrum.00665-22 PMC 바로가기 [Article Type] Article
A Qualitative Comparison of the Abbott SARS-CoV-2 IgG II Quant Assay against Commonly Used Canadian SARS-CoV-2 Enzyme Immunoassays in Blood Donor Retention Specimens, April 2020 to March 20212020년 4월부터 2021년 3월까지 헌혈자 보유 표본에서 일반적으로 사용되는 캐나다 SARS-CoV-2 효소 면역분석에 대한 Abbott SARS-CoV-2 IgG II 정량 분석의 정성적 비교Article Published on 2022-06-292022-09-11 Journal: Microbiology Spectrum [Category] COVID19(2023년), MERS, SARS, 변종, 진단, 치료기술, 치료법, [키워드] 95% CI 95% confidence interval Abbott Abbott SARS-CoV-2 IgG aggregate Analysis analyzed anti-S anti-SARS-CoV-2 anti-SARS-CoV-2 IgG Anti-spike antibody approach assays Blood donors can be used Canada Characteristics characterized Chicago collected detect ELISAs enzyme-linked immunosorbent assay enzyme-linked immunosorbent assays full-length spike glycoprotein glycoprotein gold gold standard house identify IgG IgG response IgG responses Laboratory methods comparisons nucleocapsid positive provide Qualitative qualitative test RBD Receptor binding Receptor binding domain recognizing reference standard Research retention SARS-CoV-2 SARS-CoV-2 antibody SARS-CoV-2 antigens SARS-CoV-2 variant scored sensitivity serology Seropositivity specificity specimen spike spike glycoprotein standard error the SARS-CoV-2 the strongest threshold viral antigens wild-type [DOI] 10.1128/spectrum.01134-22 PMC 바로가기 [Article Type] Article
The Detection of SARS-CoV2 Antigen in Wastewater Using an Automated Chemiluminescence Enzyme Immunoassay자동화된 화학발광 효소 면역분석법을 사용한 폐수 내 SARS-CoV2 항원 검출Article Published on 2022-06-242022-09-11 Journal: International Journal of Environmental Research an [Category] COVID19(2023년), SARS, 치료기술, [키워드] Antigen automated chemiluminescence CLEIA COVID-19 COVID-19 epidemic detect detection driving enzyme enzyme immunoassay evaluate gold gold standard immunoassay less management pandemic Pandemics qPCR reducing RT-qPCR SARS-CoV-2 SARS-CoV-2 antigen SARS-CoV-2 virus SARS-CoV2 sensitivity specificity Surveillance the SARS-CoV-2 wastewater [DOI] 10.3390/ijerph19137783 PMC 바로가기 [Article Type] Article
Evaluation of a commercial SARS-CoV-2 multiplex PCR genotyping assay for variant identification in resource-scarce settings자원이 부족한 환경에서 변이체 식별을 위한 상용 SARS-CoV-2 다중 PCR 유전자형 분석 평가Article Published on 2022-06-242022-09-11 Journal: PLoS ONE [Category] COVID19(2023년), SARS, 변종, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 African Analysis approaches Beta Beta variant beta variants characterization circulating circulating SARS-CoV-2 variant circulating variant circulating variants complement Concordance coronavirus COVID-19 Delta delta variant Delta variants demonstrated evaluated exhibited genomic sequencing genotyped genotyping globe gold gold standard multiplex Multiplex PCR offer omicron Omicron variant pathogenic variant PCR assay Real-time PCR real-time PCR assay reproducibility SARS-CoV-2 SARS-CoV-2 surveillance SARS-CoV-2 variant SARS-CoV-2 variants sensitivity South South Africa South Korea specificity specimen Spread Surveillance variant variants WGS whole genome Whole genome sequencing whole-genome sequencing [DOI] 10.1371/journal.pone.0269071 PMC 바로가기 [Article Type] Article
Clinical evaluation of the Diagnostic Analyzer for Selective Hybridization (DASH): A point-of-care PCR test for rapid detection of SARS-CoV-2 infectionDASH(Diagnostic Analyzer for Selective Hybridization) 임상 평가: SARS-CoV-2 감염의 신속한 검출을 위한 현장 진료 PCR 검사Article Published on 2022-06-162022-09-11 Journal: PLoS ONE [Category] COVID19(2023년), SARS, 치료기술, [키워드] analyzed Analyzer Antigen calculated Cepheid clinical clinical setting collected Concordance conducted correlated COVID-19 COVID-19 symptoms COVID-19 vaccine cross-sectional cross-sectional study DASH dose doses enrolled evaluated female gold gold standard hybridization information Laboratory median age mRNA nasal swab negative and positive Negative predictive value participant PCR PCR test performed POC point-of-care positive Positive predictive value Predictive Predictive value provided SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 PCR sensitivity specificity speed Swab symptomatic symptoms of COVID-19 vaccination was tested without symptom [DOI] 10.1371/journal.pone.0270060 PMC 바로가기 [Article Type] Article
Performance of the TaqMan COVID-19 Pooling Kit for detection of SARS-CoV-2 in asymptomatic and symptomatic populations무증상 및 유증상 집단에서 SARS-CoV-2 검출을 위한 TaqMan COVID-19 풀링 키트의 성능Article Published on 2022-06-102022-09-11 Journal: PLoS ONE [Category] COVID19(2023년), SARS, 치료기술, 치료법, 치료제, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Algorithm analytical sensitivity applicability Asymptomatic Asymptomatic case Asymptomatic cases Asymptomatic COVID-19 Chain Reaction China clinical clinical evidence Cohort cohorts coronavirus coronavirus disease COVID-19 curated demonstrated detect effective Evidence expected FIVE Frame gold gold standard higher education independent t-test labor lack nare nucleocapsid Open reading frame ORF ORF1ab P-value p-values pandemic PCR testing performance performed polymerase chain polymerase chain reaction pool pooling Population positive sample Prevalence Qualitative reported resource reverse transcriptase RNA RT-PCR RT-qPCR SARS-CoV-2 severe acute respiratory syndrome Coronavirus strain supported surveillance testing Swab symptomatic symptomatic and asymptomatic t-test TaqMan transcriptase Volume Wuhan [DOI] 10.1371/journal.pone.0269798 PMC 바로가기 [Article Type] Article
Emergency SARS-CoV-2 variants of concern: rapidly direct RT-qPCR detection without RNA extraction, clinical comparison, cost-effective, and high-throughput우려되는 긴급 SARS-CoV-2 변종: RNA 추출 없이 신속하게 직접 RT-qPCR 검출, 임상 비교, 비용 효율적 및 높은 처리량Article Published on 2022-06-022022-09-11 Journal: Aging (Albany NY) [Category] COVID19(2023년), SARS, 변종, 치료기술, [키워드] Accuracy acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 alternative method analytical sensitivity Chain Reaction characterized complex coronavirus coronavirus disease Coronavirus disease 2019 cost-effective diagnosis COVID-19 Diagnosis direct RT-qPCR dominant E gene Emergency Evolution faster gold gold standard High-throughput labor limit of detection mutations nasopharyngeal swabs Negative predictive value pandemic polymerase chain polymerase chain reaction positive Predictive value protocol real-time reverse transcriptase Real-time reverse transcriptase polymerase chain reaction reverse transcriptase Reverse transcriptase polymerase chain reaction RNA extraction RT-PCR RT-qPCR SARS-CoV-2 SARS-CoV-2 variant SARS-CoV-2 VoC sensitivity severe acute respiratory syndrome Coronavirus significantly Spike protein thermocyclers variant variants variants of concern [DOI] 10.18632/aging.204095 PMC 바로가기 [Article Type] Article
Validation of Serological Methods for COVID-19 and Retrospective Screening of Health Employees and Visitors to the São Paulo University Hospital, BrazilCOVID-19에 대한 혈청학적 방법의 검증 및 브라질 상파울루 대학 병원을 방문하는 의료진 및 방문객에 대한 후향적 검사Article Published on 2022-06-022022-09-11 Journal: Frontiers in Cellular and Infection Microbiology [Category] COVID19(2023년), MERS, SARS, 진단, 치료기술, [키워드] antigens approach Brazil Clinical studies collected country COVID-19 Critical diagnostic diagnostic test diagnostic tests ECLIA Elecsy Elecsys electrochemiluminescence immunoassay ELISA employee enzyme enzyme-linked immunosorbent assays epidemiological gold gold standard Health health employees house immunoassay in-house ELISA in-house ELISAs infection rates neutralization neutralization test nucleoprotein portion Receptor binding domain Recombinant protein recombinant proteins Roche Diagnostics S1 protein SARS-CoV-2 SARS-CoV-2 antibodies SARS-CoV-2 antibody SARS-CoV-2 antigens SARS-COV-2 infection SARS-CoV-2 N protein screened Screening screenings sensitivity Sensitivity and specificity serological assays serological test Serological tests serology serum sample serum samples specificity Surveillance the S1 protein the SARS-CoV-2 university university hospital vaccinated individual vaccinated individuals validation virus neutralization test Visitor was used [DOI] 10.3389/fcimb.2022.787411 PMC 바로가기 [Article Type] Article
A Single-Round Infection Fluorescent SARS-CoV-2 Neutralization Test for COVID-19 Serological Testing at a Biosafety Level-2 LaboratoryBiosafety Level-2 실험실에서 COVID-19 혈청학적 테스트를 위한 단일 라운드 감염 형광 SARS-CoV-2 중화 테스트Article Published on 2022-06-022022-09-12 Journal: Viruses [Category] COVID19(2023년), MERS, SARS, 진단, [키워드] Accuracy Antibody Response Antiviral biosafety BSL-2 BSL-2 laboratory BSL-3 facilities correlated COVID-19 COVID-19 patient evaluated fluorescent gold standard Infection lab Laboratory Large-scale testing linearity live viruses natural infection neutralization Neutralization assay neutralizing antibody Neutralizing titer plaque reduction neutralization plasma PRNT R 2 reproducibility robust SARS-CoV-2 SARS-CoV-2 virus sensitivity Sensitivity and specificity serological test serum single-round infection fluorescent SARS-CoV-2 virus specificity specimens Test Testing vaccination [DOI] 10.3390/v14061211 PMC 바로가기 [Article Type] Article